Restriction map for
pF1KB8768
(Circular) (Six-base) MAPSORT of: pF1KB8768.seq Check: 3878 from: 1 to: 3800
pF1KB8768 3800 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 452 838 452
Size: 386 3414
Acc65I G'GTAC_C
Cuts at: 816 816
Size: 3800
AclI AA'CG_TT
Cuts at: 3640 3640
Size: 3800
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 508 1625 2057 2804 3072 169
Size: 339 1117 432 747 268 897
Fragments arranged by size:
1117 897 747 432 339 268
AflIII A'CryG_T
Cuts at: 1152 2256 3233 1152
Size: 1104 977 1719
Fragments arranged by size:
1719 1104 977
AgeI A'CCGG_T
Cuts at: 2188 2188
Size: 3800
AlwNI CAG_nnn'CTG
Cuts at: 144 339 678 1105 2672 144
Size: 195 339 427 1567 1272
Fragments arranged by size:
1567 1272 427 339 195
ApaLI G'TGCA_C
Cuts at: 2570 2570
Size: 3800
ApoI r'AATT_y
Cuts at: 804 3153 3409 804
Size: 2349 256 1195
Fragments arranged by size:
2349 1195 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3800
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
AvaI C'yCGr_G
Cuts at: 428 733 820 428
Size: 305 87 3408
Fragments arranged by size:
3408 305 87
BamHI G'GATC_C
Cuts at: 825 825
Size: 3800
BanI G'GyrC_C
Cuts at: 238 604 816 1230 1478 1513 238
Size: 366 212 414 248 35 2525
Fragments arranged by size:
2525 414 366 248 212 35
BanII G_rGCy'C
Cuts at: 734 814 1844 734
Size: 80 1030 2690
Fragments arranged by size:
2690 1030 80
BbeI G_GCGC'C
Cuts at: 1234 1482 1234
Size: 248 3552
BciVI GTATCCnnnnn_n'
Cuts at: 1698 2459 3800 1698
Size: 761 1341 1698
Fragments arranged by size:
1698 1341 761
BclI T'GATC_A
Cuts at: 1320 1320
Size: 3800
BglII A'GATC_T
Cuts at: 1315 3352 1315
Size: 2037 1763
BlpI GC'TnA_GC
Cuts at: 912 912
Size: 3800
Bme1580I G_kGCm'C
Cuts at: 1425 1518 2574 1425
Size: 93 1056 2651
Fragments arranged by size:
2651 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1012 1093 1428 3572 1012
Size: 81 335 2144 1240
Fragments arranged by size:
2144 1240 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 3800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 709
Size: 3800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 976 1855 2347 2645 976
Size: 879 492 298 2131
Fragments arranged by size:
2131 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3504 3504
Size: 3800
BsaAI yAC'GTr
Cuts at: 1153 1153
Size: 3800
BsaBI GATnn'nnATC
Cuts at: 6 1314 3351 6
Size: 1308 2037 455
Fragments arranged by size:
2037 1308 455
BsaHI Gr'CG_yC
Cuts at: 1231 1479 2181 3677 1231
Size: 248 702 1496 1354
Fragments arranged by size:
1496 1354 702 248
BsaWI w'CCGG_w
Cuts at: 989 1510 2188 2462 2609 3020 989
Size: 521 678 274 147 411 1769
Fragments arranged by size:
1769 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 339 1225 1983 2560 3056 339
Size: 886 758 577 496 1083
Fragments arranged by size:
1083 886 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 352 691 253
Size: 99 339 3362
Fragments arranged by size:
3362 339 99
BsiEI CG_ry'CG
Cuts at: 104 1342 1388 2596 104
Size: 1238 46 1208 1308
Fragments arranged by size:
1308 1238 1208 46
BsiHKAI G_wGCw'C
Cuts at: 814 1592 1782 2574 814
Size: 778 190 792 2040
Fragments arranged by size:
2040 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 734 814 1425 1518 1592 1782 1844 2574 734
Size: 80 611 93 74 190 62 730 1960
Fragments arranged by size:
1960 730 611 190 93 80 74 62
BspEI T'CCGG_A
Cuts at: 3020 3020
Size: 3800
BspHI T'CATG_A
Cuts at: 297 2976 297
Size: 2679 1121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 314 539 850 1064 1366 1747 314
Size: 225 311 214 302 381 2367
Fragments arranged by size:
2367 381 311 302 225 214
BsrBI CCG'CTC
Cuts at: 50 284 2092 2146 3630 3795 50
Size: 234 1808 54 1484 165 55
Fragments arranged by size:
1808 1484 234 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1712 1712
Size: 3800
BsrFI r'CCGG_y
Cuts at: 718 1798 1979 2188 718
Size: 1080 181 209 2330
Fragments arranged by size:
2330 1080 209 181
BssSI C'ACGA_G
Cuts at: 2071 2429 2071
Size: 358 3442
BstAPI GCAn_nnn'nTGC
Cuts at: 1177 1177
Size: 3800
BstBI TT'CG_AA
Cuts at: 2161 2161
Size: 3800
BstXI CCAn_nnnn'nTGG
Cuts at: 552 552
Size: 3800
BstYI r'GATC_y
Cuts at: 1 825 1292 1315 1650 1896 2908 2994 3006
Size: 824 467 23 335 246 1012 86 12
Cuts at: 3006 3352 1
Size: 346 449
Fragments arranged by size:
1012 824 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 351 107
Size: 244 3556
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 631 1750 1931 2094 631
Size: 1119 181 163 2337
Fragments arranged by size:
2337 1119 181 163
BtsI GCAGTG_nn'
Cuts at: 1043 1043
Size: 3800
DraI TTT'AAA
Cuts at: 799 799
Size: 3800
DraIII CAC_nnn'GTG
Cuts at: 357 357
Size: 3800
DrdI GACnn_nn'nnGTC
Cuts at: 1506 2364 1506
Size: 858 2942
EaeI y'GGCC_r
Cuts at: 1385 1559 1950 1977 3322 1385
Size: 174 391 27 1345 1863
Fragments arranged by size:
1863 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1385 1385
Size: 3800
EarI CTCTTCn'nnn_
Cuts at: 476 1823 2033 476
Size: 1347 210 2243
Fragments arranged by size:
2243 1347 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2008 2318 2464 3610 2008
Size: 310 146 1146 2198
Fragments arranged by size:
2198 1146 310 146
EcoICRI GAG'CTC
Cuts at: 812 812
Size: 3800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 508 709 1625 2057 2804 3072 169
Size: 339 201 916 432 747 268 897
Fragments arranged by size:
916 897 747 432 339 268 201
EcoO109I rG'GnC_Cy
Cuts at: 939 939
Size: 3800
EcoRI G'AATT_C
Cuts at: 804 804
Size: 3800
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1819 1819
Size: 3800
FspI TGC'GCA
Cuts at: 1581 1581
Size: 3800
HaeII r_GCGC'y
Cuts at: 417 1234 1482 2504 3483 417
Size: 817 248 1022 979 734
Fragments arranged by size:
1022 979 817 734 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 727 759 1642 1672 1674 1704 727
Size: 32 883 30 2 30 2823
Fragments arranged by size:
2823 883 32 30 30 2
HincII GTy'rAC
Cuts at: 839 839
Size: 3800
KasI G'GCGC_C
Cuts at: 1230 1478 1230
Size: 248 3552
KpnI G_GTAC'C
Cuts at: 820 820
Size: 3800
MluI A'CGCG_T
Cuts at: 3233 3233
Size: 3800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2471 2655 2471
Size: 184 3616
MscI TGG'CCA
Cuts at: 1561 1561
Size: 3800
MslI CAynn'nnrTG
Cuts at: 790 1789 1916 790
Size: 999 127 2674
Fragments arranged by size:
2674 999 127
MspA1I CmG'CkG
Cuts at: 244 911 1225 1585 2598 2843 244
Size: 667 314 360 1013 245 1201
Fragments arranged by size:
1201 1013 667 360 314 245
NaeI GCC'GGC
Cuts at: 1981 1981
Size: 3800
NarI GG'CG_CC
Cuts at: 1231 1479 1231
Size: 248 3552
NcoI C'CATG_G
Cuts at: 107 107
Size: 3800
NgoMIV G'CCGG_C
Cuts at: 1979 1979
Size: 3800
NspI r_CATG'y
Cuts at: 690 853 1353 2260 690
Size: 163 500 907 2230
Fragments arranged by size:
2230 907 500 163
PciI A'CATG_T
Cuts at: 2256 2256
Size: 3800
PflMI CCAn_nnn'nTGG
Cuts at: 339 339
Size: 3800
PfoI T'CCnGG_A
Cuts at: 725 725
Size: 3800
PmeI GTTT'AAAC
Cuts at: 799 799
Size: 3800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2939 2971 2939
Size: 32 3768
PstI C_TGCA'G
Cuts at: 349 413 847 1532 349
Size: 64 434 685 2617
Fragments arranged by size:
2617 685 434 64
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3800
PvuII CAG'CTG
Cuts at: 1225 1585 1225
Size: 360 3440
RsrII CG'GwC_CG
Cuts at: 1995 1995
Size: 3800
SacI G_AGCT'C
Cuts at: 814 814
Size: 3800
SalI G'TCGA_C
Cuts at: 837 837
Size: 3800
SapI GCTCTTCn'nnn_
Cuts at: 476 1823 2033 476
Size: 1347 210 2243
Fragments arranged by size:
2243 1347 210
SbfI CC_TGCA'GG
Cuts at: 847 847
Size: 3800
ScaI AGT'ACT
Cuts at: 3317 3317
Size: 3800
SfcI C'TryA_G
Cuts at: 33 345 409 843 1528 2521 2712 33
Size: 312 64 434 685 993 191 1121
Fragments arranged by size:
1121 993 685 434 312 191 64
SfoI GGC'GCC
Cuts at: 1232 1480 1232
Size: 248 3552
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
SmaI CCC'GGG
Cuts at: 430 822 430
Size: 392 3408
SmlI C'TyrA_G
Cuts at: 955 1870 2362 2624 955
Size: 915 492 262 2131
Fragments arranged by size:
2131 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3800
SphI G_CATG'C
Cuts at: 853 1353 853
Size: 500 3300
StuI AGG'CCT
Cuts at: 493 493
Size: 3800
StyI C'CwwG_G
Cuts at: 107 131 934 1288 107
Size: 24 803 354 2619
Fragments arranged by size:
2619 803 354 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 592 673 1383 1516 1975 2184 592
Size: 81 710 133 459 209 2208
Fragments arranged by size:
2208 710 459 209 133 81
TatI w'GTAC_w
Cuts at: 3315 3315
Size: 3800
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 3768
Tth111I GACn'n_nGTC
Cuts at: 1597 1597
Size: 3800
XbaI T'CTAG_A
Cuts at: 831 831
Size: 3800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 552 615 1295 552
Size: 63 680 3057
Fragments arranged by size:
3057 680 63
XmaI C'CCGG_G
Cuts at: 428 820 428
Size: 392 3408
XmnI GAAnn'nnTTC
Cuts at: 493 493
Size: 3800
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI