Restriction map for
pF1KB8769
(Circular) (Six-base) MAPSORT of: pF1KB8769.seq Check: 4738 from: 1 to: 3812
pF1KB8769 3812 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 850 850
Size: 3812
Acc65I G'GTAC_C
Cuts at: 828 828
Size: 3812
AclI AA'CG_TT
Cuts at: 3652 3652
Size: 3812
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 541 1637 2069 2816 3084 285
Size: 256 1096 432 747 268 1013
Fragments arranged by size:
1096 1013 747 432 268 256
AfeI AGC'GCT
Cuts at: 778 778
Size: 3812
AflIII A'CryG_T
Cuts at: 1164 2268 3245 1164
Size: 1104 977 1731
Fragments arranged by size:
1731 1104 977
AgeI A'CCGG_T
Cuts at: 2200 2200
Size: 3812
AlwNI CAG_nnn'CTG
Cuts at: 357 1117 2684 357
Size: 760 1567 1485
Fragments arranged by size:
1567 1485 760
ApaI G_GGCC'C
Cuts at: 755 755
Size: 3812
ApaLI G'TGCA_C
Cuts at: 2582 2582
Size: 3812
ApoI r'AATT_y
Cuts at: 275 816 3165 3421 275
Size: 541 2349 256 666
Fragments arranged by size:
2349 666 541 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3812
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
AvaI C'yCGr_G
Cuts at: 794 832 794
Size: 38 3774
BamHI G'GATC_C
Cuts at: 837 837
Size: 3812
BanI G'GyrC_C
Cuts at: 828 1242 1490 1525 828
Size: 414 248 35 3115
Fragments arranged by size:
3115 414 248 35
BanII G_rGCy'C
Cuts at: 615 755 826 1856 615
Size: 140 71 1030 2571
Fragments arranged by size:
2571 1030 140 71
BbeI G_GCGC'C
Cuts at: 1246 1494 1246
Size: 248 3564
BciVI GTATCCnnnnn_n'
Cuts at: 1710 2471 3812 1710
Size: 761 1341 1710
Fragments arranged by size:
1710 1341 761
BclI T'GATC_A
Cuts at: 1332 1332
Size: 3812
BglI GCCn_nnn'nGGC
Cuts at: 126 126
Size: 3812
BglII A'GATC_T
Cuts at: 1327 3364 1327
Size: 2037 1775
BlpI GC'TnA_GC
Cuts at: 924 924
Size: 3812
Bme1580I G_kGCm'C
Cuts at: 755 1437 1530 2586 755
Size: 682 93 1056 1981
Fragments arranged by size:
1981 1056 682 93
BmrI ACTGGGnnnn_n'
Cuts at: 203 1024 1105 1440 3584 203
Size: 821 81 335 2144 431
Fragments arranged by size:
2144 821 431 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 3812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 988 1867 2359 2657 640
Size: 348 879 492 298 1795
Fragments arranged by size:
1795 879 492 348 298
BsaI GGTCTCn'nnnn_
Cuts at: 372 536 3516 372
Size: 164 2980 668
Fragments arranged by size:
2980 668 164
BsaAI yAC'GTr
Cuts at: 1165 1165
Size: 3812
BsaBI GATnn'nnATC
Cuts at: 6 1326 3363 6
Size: 1320 2037 455
Fragments arranged by size:
2037 1320 455
BsaHI Gr'CG_yC
Cuts at: 1243 1491 2193 3689 1243
Size: 248 702 1496 1366
Fragments arranged by size:
1496 1366 702 248
BsaWI w'CCGG_w
Cuts at: 185 545 1001 1522 2200 2474 2621 3032 185
Size: 360 456 521 678 274 147 411 965
Fragments arranged by size:
965 678 521 456 411 360 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 388 409 388
Size: 21 3791
BseYI C'CCAG_C
Cuts at: 1237 1995 2572 3068 1237
Size: 758 577 496 1981
Fragments arranged by size:
1981 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1354 1400 2608 104
Size: 1250 46 1208 1308
Fragments arranged by size:
1308 1250 1208 46
BsiHKAI G_wGCw'C
Cuts at: 826 1604 1794 2586 826
Size: 778 190 792 2052
Fragments arranged by size:
2052 792 778 190
BsmI GAATG_Cn'
Cuts at: 678 678
Size: 3812
Bsp1286I G_dGCh'C
Cuts at: 615 755 826 1437 1530 1604 1794 1856 2586
Size: 140 71 611 93 74 190 62 730
Cuts at: 2586 615
Size: 1841
Fragments arranged by size:
1841 730 611 190 140 93 74 71
62
BspEI T'CCGG_A
Cuts at: 3032 3032
Size: 3812
BspHI T'CATG_A
Cuts at: 2988 2988
Size: 3812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 862 1076 1378 1759 862
Size: 214 302 381 2915
Fragments arranged by size:
2915 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2104 2158 3642 3807 50
Size: 2054 54 1484 165 55
Fragments arranged by size:
2054 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 722 1724 722
Size: 1002 2810
BsrFI r'CCGG_y
Cuts at: 1810 1991 2200 1810
Size: 181 209 3422
Fragments arranged by size:
3422 209 181
BssSI C'ACGA_G
Cuts at: 2083 2441 2083
Size: 358 3454
BstAPI GCAn_nnn'nTGC
Cuts at: 240 780 1189 240
Size: 540 409 2863
Fragments arranged by size:
2863 540 409
BstBI TT'CG_AA
Cuts at: 2173 2173
Size: 3812
BstYI r'GATC_y
Cuts at: 1 332 353 837 1304 1327 1662 1908 2920
Size: 331 21 484 467 23 335 246 1012
Cuts at: 2920 3006 3018 3364 1
Size: 86 12 346 449
Fragments arranged by size:
1012 484 467 449 346 335 331 246
86 23 21 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3812
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1762 1943 2106 1762
Size: 181 163 3468
Fragments arranged by size:
3468 181 163
BtsI GCAGTG_nn'
Cuts at: 1055 1055
Size: 3812
DraI TTT'AAA
Cuts at: 811 811
Size: 3812
DrdI GACnn_nn'nnGTC
Cuts at: 1518 2376 1518
Size: 858 2954
EaeI y'GGCC_r
Cuts at: 110 717 1397 1571 1962 1989 3334 110
Size: 607 680 174 391 27 1345 588
Fragments arranged by size:
1345 680 607 588 391 174 27
EagI C'GGCC_G
Cuts at: 1397 1397
Size: 3812
EarI CTCTTCn'nnn_
Cuts at: 260 302 365 386 452 662 683 1835 2045
Size: 42 63 21 66 210 21 1152 210
Cuts at: 2045 260
Size: 2027
Fragments arranged by size:
2027 1152 210 210 66 63 42 21
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2020 2330 2476 3622 2020
Size: 310 146 1146 2210
Fragments arranged by size:
2210 1146 310 146
EcoICRI GAG'CTC
Cuts at: 824 824
Size: 3812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 541 1637 2069 2816 3084 285
Size: 256 1096 432 747 268 1013
Fragments arranged by size:
1096 1013 747 432 268 256
EcoNI CCTnn'n_nnAGG
Cuts at: 556 577 619 661 682 556
Size: 21 42 42 21 3686
Fragments arranged by size:
3686 42 42 21 21
EcoO109I rG'GnC_Cy
Cuts at: 570 591 695 752 951 570
Size: 21 104 57 199 3431
Fragments arranged by size:
3431 199 104 57 21
EcoRI G'AATT_C
Cuts at: 816 816
Size: 3812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1831 1831
Size: 3812
FspI TGC'GCA
Cuts at: 1593 1593
Size: 3812
HaeII r_GCGC'y
Cuts at: 780 1246 1494 2516 3495 780
Size: 466 248 1022 979 1097
Fragments arranged by size:
1097 1022 979 466 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 317 349 406 438 637 669 1654 1684 1686
Size: 32 57 32 199 32 985 30 2
Cuts at: 1686 1716 317
Size: 30 2413
Fragments arranged by size:
2413 985 199 57 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 851 851
Size: 3812
KasI G'GCGC_C
Cuts at: 1242 1490 1242
Size: 248 3564
KpnI G_GTAC'C
Cuts at: 832 832
Size: 3812
MluI A'CGCG_T
Cuts at: 3245 3245
Size: 3812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2483 2667 2483
Size: 184 3628
MscI TGG'CCA
Cuts at: 112 719 1573 112
Size: 607 854 2351
Fragments arranged by size:
2351 854 607
MslI CAynn'nnrTG
Cuts at: 1801 1928 1801
Size: 127 3685
MspA1I CmG'CkG
Cuts at: 923 1237 1597 2610 2855 923
Size: 314 360 1013 245 1880
Fragments arranged by size:
1880 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1993 1993
Size: 3812
NarI GG'CG_CC
Cuts at: 1243 1491 1243
Size: 248 3564
NcoI C'CATG_G
Cuts at: 107 107
Size: 3812
NgoMIV G'CCGG_C
Cuts at: 1991 1991
Size: 3812
NruI TCG'CGA
Cuts at: 263 263
Size: 3812
NspI r_CATG'y
Cuts at: 865 1365 2272 865
Size: 500 907 2405
Fragments arranged by size:
2405 907 500
PciI A'CATG_T
Cuts at: 2268 2268
Size: 3812
PflMI CCAn_nnn'nTGG
Cuts at: 357 357
Size: 3812
PmeI GTTT'AAAC
Cuts at: 811 811
Size: 3812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2951 2983 2951
Size: 32 3780
PspOMI G'GGCC_C
Cuts at: 751 751
Size: 3812
PstI C_TGCA'G
Cuts at: 245 859 1544 245
Size: 614 685 2513
Fragments arranged by size:
2513 685 614
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3812
PvuII CAG'CTG
Cuts at: 1237 1597 1237
Size: 360 3452
RsrII CG'GwC_CG
Cuts at: 2007 2007
Size: 3812
SacI G_AGCT'C
Cuts at: 826 826
Size: 3812
SalI G'TCGA_C
Cuts at: 849 849
Size: 3812
SapI GCTCTTCn'nnn_
Cuts at: 1835 2045 1835
Size: 210 3602
SbfI CC_TGCA'GG
Cuts at: 859 859
Size: 3812
ScaI AGT'ACT
Cuts at: 3329 3329
Size: 3812
SfcI C'TryA_G
Cuts at: 33 241 855 1540 2533 2724 33
Size: 208 614 685 993 191 1121
Fragments arranged by size:
1121 993 685 614 208 191
SfoI GGC'GCC
Cuts at: 1244 1492 1244
Size: 248 3564
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
SmaI CCC'GGG
Cuts at: 834 834
Size: 3812
SmlI C'TyrA_G
Cuts at: 619 967 1882 2374 2636 619
Size: 348 915 492 262 1795
Fragments arranged by size:
1795 915 492 348 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3812
SphI G_CATG'C
Cuts at: 865 1365 865
Size: 500 3312
StuI AGG'CCT
Cuts at: 508 508
Size: 3812
StyI C'CwwG_G
Cuts at: 107 946 1300 107
Size: 839 354 2619
Fragments arranged by size:
2619 839 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1395 1528 1987 2196 1395
Size: 133 459 209 3011
Fragments arranged by size:
3011 459 209 133
TatI w'GTAC_w
Cuts at: 3327 3327
Size: 3812
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 298 330 3329 3361 298
Size: 32 2999 32 749
Fragments arranged by size:
2999 749 32 32
Tth111I GACn'n_nGTC
Cuts at: 1609 1609
Size: 3812
XbaI T'CTAG_A
Cuts at: 843 843
Size: 3812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1307 1307
Size: 3812
XmaI C'CCGG_G
Cuts at: 832 832
Size: 3812
XmnI GAAnn'nnTTC
Cuts at: 403 466 403
Size: 63 3749
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI