Restriction map for
pF1KB8770
(Circular) (Six-base) MAPSORT of: pF1KB8770.seq Check: 2604 from: 1 to: 3815
pF1KB8770 3815 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 853 853
Size: 3815
Acc65I G'GTAC_C
Cuts at: 831 831
Size: 3815
AclI AA'CG_TT
Cuts at: 3655 3655
Size: 3815
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1640 2072 2819 3087 1640
Size: 432 747 268 2368
Fragments arranged by size:
2368 747 432 268
AflII C'TTAA_G
Cuts at: 550 550
Size: 3815
AflIII A'CryG_T
Cuts at: 800 1167 2271 3248 800
Size: 367 1104 977 1367
Fragments arranged by size:
1367 1104 977 367
AgeI A'CCGG_T
Cuts at: 2203 2203
Size: 3815
AlwNI CAG_nnn'CTG
Cuts at: 1120 2687 1120
Size: 1567 2248
ApaLI G'TGCA_C
Cuts at: 360 2585 360
Size: 2225 1590
ApoI r'AATT_y
Cuts at: 379 819 3168 3424 379
Size: 440 2349 256 770
Fragments arranged by size:
2349 770 440 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3815
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3815
AvaI C'yCGr_G
Cuts at: 268 691 835 268
Size: 423 144 3248
Fragments arranged by size:
3248 423 144
BamHI G'GATC_C
Cuts at: 840 840
Size: 3815
BanI G'GyrC_C
Cuts at: 831 1245 1493 1528 831
Size: 414 248 35 3118
Fragments arranged by size:
3118 414 248 35
BanII G_rGCy'C
Cuts at: 766 829 1859 766
Size: 63 1030 2722
Fragments arranged by size:
2722 1030 63
BbeI G_GCGC'C
Cuts at: 1249 1497 1249
Size: 248 3567
BbsI GAAGACnn'nnnn_
Cuts at: 736 736
Size: 3815
BbvCI CC'TCA_GC
Cuts at: 784 784
Size: 3815
BciVI GTATCCnnnnn_n'
Cuts at: 1713 2474 3815 1713
Size: 761 1341 1713
Fragments arranged by size:
1713 1341 761
BclI T'GATC_A
Cuts at: 1335 1335
Size: 3815
BglII A'GATC_T
Cuts at: 113 1330 3367 113
Size: 1217 2037 561
Fragments arranged by size:
2037 1217 561
BlpI GC'TnA_GC
Cuts at: 927 927
Size: 3815
Bme1580I G_kGCm'C
Cuts at: 364 1440 1533 2589 364
Size: 1076 93 1056 1590
Fragments arranged by size:
1590 1076 1056 93
BmgBI CAC'GTC
Cuts at: 803 803
Size: 3815
BmrI ACTGGGnnnn_n'
Cuts at: 1027 1108 1443 3587 1027
Size: 81 335 2144 1255
Fragments arranged by size:
2144 1255 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 360 2182 360
Size: 1822 1993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 760
Size: 3815
Bpu10I CC'TnA_GC
Cuts at: 155 784 155
Size: 629 3186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 409 991 1870 2362 2660 334
Size: 75 582 879 492 298 1489
Fragments arranged by size:
1489 879 582 492 298 75
BsaI GGTCTCn'nnnn_
Cuts at: 219 634 3519 219
Size: 415 2885 515
Fragments arranged by size:
2885 515 415
BsaAI yAC'GTr
Cuts at: 1168 1168
Size: 3815
BsaBI GATnn'nnATC
Cuts at: 6 1329 3366 6
Size: 1323 2037 455
Fragments arranged by size:
2037 1323 455
BsaHI Gr'CG_yC
Cuts at: 1246 1494 2196 3692 1246
Size: 248 702 1496 1369
Fragments arranged by size:
1496 1369 702 248
BsaWI w'CCGG_w
Cuts at: 1004 1525 2203 2477 2624 3035 1004
Size: 521 678 274 147 411 1784
Fragments arranged by size:
1784 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 672 702 672
Size: 30 3785
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 349 349
Size: 3815
BseYI C'CCAG_C
Cuts at: 1240 1998 2575 3071 1240
Size: 758 577 496 1984
Fragments arranged by size:
1984 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 543 756 814 543
Size: 213 58 3544
Fragments arranged by size:
3544 213 58
BsiEI CG_ry'CG
Cuts at: 104 1357 1403 2611 104
Size: 1253 46 1208 1308
Fragments arranged by size:
1308 1253 1208 46
BsiHKAI G_wGCw'C
Cuts at: 164 364 626 766 829 1607 1797 2589 164
Size: 200 262 140 63 778 190 792 1390
Fragments arranged by size:
1390 792 778 262 200 190 140 63
Bsp1286I G_dGCh'C
Cuts at: 164 364 626 766 829 1440 1533 1607 1797
Size: 200 262 140 63 611 93 74 190
Cuts at: 1797 1859 2589 164
Size: 62 730 1390
Fragments arranged by size:
1390 730 611 262 200 190 140 93
74 63 62
BspEI T'CCGG_A
Cuts at: 3035 3035
Size: 3815
BspHI T'CATG_A
Cuts at: 2991 2991
Size: 3815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 865 1079 1381 1762 865
Size: 214 302 381 2918
Fragments arranged by size:
2918 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2107 2161 3645 3810 50
Size: 2057 54 1484 165 55
Fragments arranged by size:
2057 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1727 1727
Size: 3815
BsrFI r'CCGG_y
Cuts at: 397 1813 1994 2203 397
Size: 1416 181 209 2009
Fragments arranged by size:
2009 1416 209 181
BssSI C'ACGA_G
Cuts at: 2086 2444 2086
Size: 358 3457
BstAPI GCAn_nnn'nTGC
Cuts at: 1192 1192
Size: 3815
BstBI TT'CG_AA
Cuts at: 2176 2176
Size: 3815
BstYI r'GATC_y
Cuts at: 1 113 840 1307 1330 1665 1911 2923 3009
Size: 112 727 467 23 335 246 1012 86
Cuts at: 3009 3021 3367 1
Size: 12 346 449
Fragments arranged by size:
1012 727 467 449 346 335 246 112
86 23 12
BtgI C'CryG_G
Cuts at: 674 674
Size: 3815
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1765 1946 2109 1765
Size: 181 163 3471
Fragments arranged by size:
3471 181 163
BtsI GCAGTG_nn'
Cuts at: 668 1058 668
Size: 390 3425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 555 590 555
Size: 35 3780
DraI TTT'AAA
Cuts at: 814 814
Size: 3815
DrdI GACnn_nn'nnGTC
Cuts at: 1521 2379 1521
Size: 858 2957
EaeI y'GGCC_r
Cuts at: 677 1400 1574 1965 1992 3337 677
Size: 723 174 391 27 1345 1155
Fragments arranged by size:
1345 1155 723 391 174 27
EagI C'GGCC_G
Cuts at: 1400 1400
Size: 3815
EarI CTCTTCn'nnn_
Cuts at: 1838 2048 1838
Size: 210 3605
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2023 2333 2479 3625 2023
Size: 310 146 1146 2213
Fragments arranged by size:
2213 1146 310 146
EcoICRI GAG'CTC
Cuts at: 764 827 764
Size: 63 3752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 1640 2072 2819 3087 760
Size: 880 432 747 268 1488
Fragments arranged by size:
1488 880 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 169 169
Size: 3815
EcoO109I rG'GnC_Cy
Cuts at: 705 954 705
Size: 249 3566
EcoRI G'AATT_C
Cuts at: 819 819
Size: 3815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1834 1834
Size: 3815
FspI TGC'GCA
Cuts at: 1596 1596
Size: 3815
HaeII r_GCGC'y
Cuts at: 1249 1497 2519 3498 1249
Size: 248 1022 979 1566
Fragments arranged by size:
1566 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1657 1687 1689 1719 1657
Size: 30 2 30 3753
Fragments arranged by size:
3753 30 30 2
HincII GTy'rAC
Cuts at: 854 854
Size: 3815
KasI G'GCGC_C
Cuts at: 1245 1493 1245
Size: 248 3567
KpnI G_GTAC'C
Cuts at: 835 835
Size: 3815
MfeI C'AATT_G
Cuts at: 367 367
Size: 3815
MluI A'CGCG_T
Cuts at: 3248 3248
Size: 3815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 430 2486 2670 430
Size: 2056 184 1575
Fragments arranged by size:
2056 1575 184
MscI TGG'CCA
Cuts at: 679 1576 679
Size: 897 2918
MslI CAynn'nnrTG
Cuts at: 1804 1931 1804
Size: 127 3688
MspA1I CmG'CkG
Cuts at: 505 926 1240 1600 2613 2858 505
Size: 421 314 360 1013 245 1462
Fragments arranged by size:
1462 1013 421 360 314 245
NaeI GCC'GGC
Cuts at: 399 1996 399
Size: 1597 2218
NarI GG'CG_CC
Cuts at: 1246 1494 1246
Size: 248 3567
NcoI C'CATG_G
Cuts at: 674 674
Size: 3815
NgoMIV G'CCGG_C
Cuts at: 397 1994 397
Size: 1597 2218
NspI r_CATG'y
Cuts at: 868 1368 2275 868
Size: 500 907 2408
Fragments arranged by size:
2408 907 500
PasI CC'CwG_GG
Cuts at: 617 617
Size: 3815
PciI A'CATG_T
Cuts at: 2271 2271
Size: 3815
PflMI CCAn_nnn'nTGG
Cuts at: 675 675
Size: 3815
PmeI GTTT'AAAC
Cuts at: 814 814
Size: 3815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2954 2986 2954
Size: 32 3783
PpuMI rG'GwC_Cy
Cuts at: 705 705
Size: 3815
PstI C_TGCA'G
Cuts at: 313 862 1547 313
Size: 549 685 2581
Fragments arranged by size:
2581 685 549
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3815
PvuII CAG'CTG
Cuts at: 505 1240 1600 505
Size: 735 360 2720
Fragments arranged by size:
2720 735 360
RsrII CG'GwC_CG
Cuts at: 2010 2010
Size: 3815
SacI G_AGCT'C
Cuts at: 766 829 766
Size: 63 3752
SalI G'TCGA_C
Cuts at: 852 852
Size: 3815
SapI GCTCTTCn'nnn_
Cuts at: 1838 2048 1838
Size: 210 3605
SbfI CC_TGCA'GG
Cuts at: 862 862
Size: 3815
ScaI AGT'ACT
Cuts at: 3332 3332
Size: 3815
SfcI C'TryA_G
Cuts at: 33 117 309 486 858 1543 2536 2727 33
Size: 84 192 177 372 685 993 191 1121
Fragments arranged by size:
1121 993 685 372 192 191 177 84
SfoI GGC'GCC
Cuts at: 1247 1495 1247
Size: 248 3567
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3815
SmaI CCC'GGG
Cuts at: 270 837 270
Size: 567 3248
SmlI C'TyrA_G
Cuts at: 349 424 550 970 1885 2377 2639 349
Size: 75 126 420 915 492 262 1525
Fragments arranged by size:
1525 915 492 420 262 126 75
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3815
SphI G_CATG'C
Cuts at: 868 1368 868
Size: 500 3315
StyI C'CwwG_G
Cuts at: 674 949 1303 674
Size: 275 354 3186
Fragments arranged by size:
3186 354 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 259 1398 1531 1990 2199 259
Size: 1139 133 459 209 1875
Fragments arranged by size:
1875 1139 459 209 133
TatI w'GTAC_w
Cuts at: 3330 3330
Size: 3815
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 672 704 3332 3364 672
Size: 32 2628 32 1123
Fragments arranged by size:
2628 1123 32 32
Tth111I GACn'n_nGTC
Cuts at: 1612 1612
Size: 3815
XbaI T'CTAG_A
Cuts at: 846 846
Size: 3815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1310 1310
Size: 3815
XmaI C'CCGG_G
Cuts at: 268 835 268
Size: 567 3248
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI