Restriction map for
pF1KB8771
(Circular) (Six-base) MAPSORT of: pF1KB8771.seq Check: 8639 from: 1 to: 3815
pF1KB8771 3815 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 319 321 447 319
Size: 2 126 3687
Fragments arranged by size:
3687 126 2
AccI GT'mk_AC
Cuts at: 853 853
Size: 3815
Acc65I G'GTAC_C
Cuts at: 831 831
Size: 3815
AclI AA'CG_TT
Cuts at: 3655 3655
Size: 3815
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1640 2072 2819 3087 1640
Size: 432 747 268 2368
Fragments arranged by size:
2368 747 432 268
AflIII A'CryG_T
Cuts at: 1167 2271 3248 1167
Size: 1104 977 1734
Fragments arranged by size:
1734 1104 977
AgeI A'CCGG_T
Cuts at: 2203 2203
Size: 3815
AlwNI CAG_nnn'CTG
Cuts at: 1120 2687 1120
Size: 1567 2248
ApaI G_GGCC'C
Cuts at: 735 735
Size: 3815
ApaLI G'TGCA_C
Cuts at: 2585 2585
Size: 3815
ApoI r'AATT_y
Cuts at: 819 3168 3424 819
Size: 2349 256 1210
Fragments arranged by size:
2349 1210 256
AscI GG'CGCG_CC
Cuts at: 496 649 496
Size: 153 3662
AseI AT'TA_AT
Cuts at: 20 20
Size: 3815
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3815
AvaI C'yCGr_G
Cuts at: 283 345 481 766 835 283
Size: 62 136 285 69 3263
Fragments arranged by size:
3263 285 136 69 62
BamHI G'GATC_C
Cuts at: 840 840
Size: 3815
BanI G'GyrC_C
Cuts at: 201 234 270 278 380 421 718 831 1245
Size: 33 36 8 102 41 297 113 414
Cuts at: 1245 1493 1528 201
Size: 248 35 2488
Fragments arranged by size:
2488 414 297 248 113 102 41 36
35 33 8
BanII G_rGCy'C
Cuts at: 433 735 829 1859 433
Size: 302 94 1030 2389
Fragments arranged by size:
2389 1030 302 94
BbeI G_GCGC'C
Cuts at: 205 238 274 282 425 722 1249 1497 205
Size: 33 36 8 143 297 527 248 2523
Fragments arranged by size:
2523 527 297 248 143 36 33 8
BciVI GTATCCnnnnn_n'
Cuts at: 1713 2474 3815 1713
Size: 761 1341 1713
Fragments arranged by size:
1713 1341 761
BclI T'GATC_A
Cuts at: 1335 1335
Size: 3815
BglI GCCn_nnn'nGGC
Cuts at: 716 716
Size: 3815
BglII A'GATC_T
Cuts at: 1330 3367 1330
Size: 2037 1778
BlpI GC'TnA_GC
Cuts at: 927 927
Size: 3815
Bme1580I G_kGCm'C
Cuts at: 735 1440 1533 2589 735
Size: 705 93 1056 1961
Fragments arranged by size:
1961 1056 705 93
BmrI ACTGGGnnnn_n'
Cuts at: 202 312 501 1027 1108 1443 3587 202
Size: 110 189 526 81 335 2144 430
Fragments arranged by size:
2144 526 430 335 189 110 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 3815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 577 619 430
Size: 147 42 3626
Fragments arranged by size:
3626 147 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 991 1870 2362 2660 739
Size: 252 879 492 298 1894
Fragments arranged by size:
1894 879 492 298 252
BsaI GGTCTCn'nnnn_
Cuts at: 3519 3519
Size: 3815
BsaAI yAC'GTr
Cuts at: 1168 1168
Size: 3815
BsaBI GATnn'nnATC
Cuts at: 6 1329 3366 6
Size: 1323 2037 455
Fragments arranged by size:
2037 1323 455
BsaHI Gr'CG_yC
Cuts at: 202 208 214 235 271 279 422 719 1246
Size: 6 6 21 36 8 143 297 527
Cuts at: 1246 1494 2196 3692 202
Size: 248 702 1496 325
Fragments arranged by size:
1496 702 527 325 297 248 143 36
21 8 6 6
BsaWI w'CCGG_w
Cuts at: 1004 1525 2203 2477 2624 3035 1004
Size: 521 678 274 147 411 1784
Fragments arranged by size:
1784 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 402 126
Size: 276 3539
BseYI C'CCAG_C
Cuts at: 728 1240 1998 2575 3071 728
Size: 512 758 577 496 1472
Fragments arranged by size:
1472 758 577 512 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 114
Size: 3815
BsiEI CG_ry'CG
Cuts at: 104 225 1357 1403 2611 104
Size: 121 1132 46 1208 1308
Fragments arranged by size:
1308 1208 1132 121 46
BsiHKAI G_wGCw'C
Cuts at: 433 829 1607 1797 2589 433
Size: 396 778 190 792 1659
Fragments arranged by size:
1659 792 778 396 190
Bsp1286I G_dGCh'C
Cuts at: 433 735 829 1440 1533 1607 1797 1859 2589
Size: 302 94 611 93 74 190 62 730
Cuts at: 2589 433
Size: 1659
Fragments arranged by size:
1659 730 611 302 190 94 93 74
62
BspEI T'CCGG_A
Cuts at: 3035 3035
Size: 3815
BspHI T'CATG_A
Cuts at: 2991 2991
Size: 3815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 319 321 447 865 1079 1381 1762 319
Size: 2 126 418 214 302 381 2372
Fragments arranged by size:
2372 418 381 302 214 126 2
BsrBI CCG'CTC
Cuts at: 50 549 561 603 672 753 2107 2161 3645
Size: 499 12 42 69 81 1354 54 1484
Cuts at: 3645 3810 50
Size: 165 55
Fragments arranged by size:
1484 1354 499 165 81 69 55 54
42 12
BsrDI GCAATG_nn'
Cuts at: 1727 1727
Size: 3815
BsrFI r'CCGG_y
Cuts at: 123 198 398 539 1813 1994 2203 123
Size: 75 200 141 1274 181 209 1735
Fragments arranged by size:
1735 1274 209 200 181 141 75
BssHII G'CGCG_C
Cuts at: 392 496 518 591 633 649 392
Size: 104 22 73 42 16 3558
Fragments arranged by size:
3558 104 73 42 22 16
BssSI C'ACGA_G
Cuts at: 2086 2444 2086
Size: 358 3457
BstAPI GCAn_nnn'nTGC
Cuts at: 1192 1192
Size: 3815
BstBI TT'CG_AA
Cuts at: 2176 2176
Size: 3815
BstYI r'GATC_y
Cuts at: 1 840 1307 1330 1665 1911 2923 3009 3021
Size: 839 467 23 335 246 1012 86 12
Cuts at: 3021 3367 1
Size: 346 449
Fragments arranged by size:
1012 839 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 385 385
Size: 3815
BtgI C'CryG_G
Cuts at: 134 147 339 575 638 134
Size: 13 192 236 63 3311
Fragments arranged by size:
3311 236 192 63 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1765 1946 2109 1765
Size: 181 163 3471
Fragments arranged by size:
3471 181 163
BtsI GCAGTG_nn'
Cuts at: 1058 1058
Size: 3815
DraI TTT'AAA
Cuts at: 814 814
Size: 3815
DrdI GACnn_nn'nnGTC
Cuts at: 787 1521 2379 787
Size: 734 858 2223
Fragments arranged by size:
2223 858 734
EaeI y'GGCC_r
Cuts at: 222 524 572 1400 1574 1965 1992 3337 222
Size: 302 48 828 174 391 27 1345 700
Fragments arranged by size:
1345 828 700 391 302 174 48 27
EagI C'GGCC_G
Cuts at: 222 1400 222
Size: 1178 2637
EarI CTCTTCn'nnn_
Cuts at: 623 1838 2048 623
Size: 1215 210 2390
Fragments arranged by size:
2390 1215 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 624 2023 2333 2479 3625 624
Size: 1399 310 146 1146 814
Fragments arranged by size:
1399 1146 814 310 146
EcoICRI GAG'CTC
Cuts at: 431 827 431
Size: 396 3419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 577 619 1640 2072 2819 3087 430
Size: 147 42 1021 432 747 268 1158
Fragments arranged by size:
1158 1021 747 432 268 147 42
EcoO109I rG'GnC_Cy
Cuts at: 362 732 801 954 362
Size: 370 69 153 3223
Fragments arranged by size:
3223 370 153 69
EcoRI G'AATT_C
Cuts at: 819 819
Size: 3815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1834 1834
Size: 3815
FspI TGC'GCA
Cuts at: 1596 1596
Size: 3815
HaeII r_GCGC'y
Cuts at: 205 238 274 282 425 619 682 722 1249
Size: 33 36 8 143 194 63 40 527
Cuts at: 1249 1497 2519 3498 205
Size: 248 1022 979 522
Fragments arranged by size:
1022 979 527 522 248 194 143 63
40 36 33 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1657 1687 1689 1719 1657
Size: 30 2 30 3753
Fragments arranged by size:
3753 30 30 2
HincII GTy'rAC
Cuts at: 854 854
Size: 3815
KasI G'GCGC_C
Cuts at: 201 234 270 278 421 718 1245 1493 201
Size: 33 36 8 143 297 527 248 2523
Fragments arranged by size:
2523 527 297 248 143 36 33 8
KpnI G_GTAC'C
Cuts at: 835 835
Size: 3815
MluI A'CGCG_T
Cuts at: 3248 3248
Size: 3815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2486 2670 2486
Size: 184 3631
MscI TGG'CCA
Cuts at: 1576 1576
Size: 3815
MslI CAynn'nnrTG
Cuts at: 1804 1931 1804
Size: 127 3688
MspA1I CmG'CkG
Cuts at: 149 341 419 472 640 739 926 1240 1600
Size: 192 78 53 168 99 187 314 360
Cuts at: 1600 2613 2858 149
Size: 1013 245 1106
Fragments arranged by size:
1106 1013 360 314 245 192 187 168
99 78 53
NaeI GCC'GGC
Cuts at: 125 200 541 1996 125
Size: 75 341 1455 1944
Fragments arranged by size:
1944 1455 341 75
NarI GG'CG_CC
Cuts at: 202 235 271 279 422 719 1246 1494 202
Size: 33 36 8 143 297 527 248 2523
Fragments arranged by size:
2523 527 297 248 143 36 33 8
NcoI C'CATG_G
Cuts at: 134 134
Size: 3815
NgoMIV G'CCGG_C
Cuts at: 123 198 539 1994 123
Size: 75 341 1455 1944
Fragments arranged by size:
1944 1455 341 75
NspI r_CATG'y
Cuts at: 868 1368 2275 868
Size: 500 907 2408
Fragments arranged by size:
2408 907 500
PasI CC'CwG_GG
Cuts at: 723 723
Size: 3815
PciI A'CATG_T
Cuts at: 2271 2271
Size: 3815
PfoI T'CCnGG_A
Cuts at: 186 186
Size: 3815
PmeI GTTT'AAAC
Cuts at: 814 814
Size: 3815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2954 2986 2954
Size: 32 3783
PpuMI rG'GwC_Cy
Cuts at: 362 801 362
Size: 439 3376
PspOMI G'GGCC_C
Cuts at: 731 731
Size: 3815
PstI C_TGCA'G
Cuts at: 623 665 686 862 1547 623
Size: 42 21 176 685 2891
Fragments arranged by size:
2891 685 176 42 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3815
PvuII CAG'CTG
Cuts at: 1240 1600 1240
Size: 360 3455
RsrII CG'GwC_CG
Cuts at: 2010 2010
Size: 3815
SacI G_AGCT'C
Cuts at: 433 829 433
Size: 396 3419
SacII CC_GC'GG
Cuts at: 150 342 641 150
Size: 192 299 3324
Fragments arranged by size:
3324 299 192
SalI G'TCGA_C
Cuts at: 852 852
Size: 3815
SapI GCTCTTCn'nnn_
Cuts at: 623 1838 2048 623
Size: 1215 210 2390
Fragments arranged by size:
2390 1215 210
SbfI CC_TGCA'GG
Cuts at: 862 862
Size: 3815
ScaI AGT'ACT
Cuts at: 3332 3332
Size: 3815
SfcI C'TryA_G
Cuts at: 33 619 661 682 858 1543 2536 2727 33
Size: 586 42 21 176 685 993 191 1121
Fragments arranged by size:
1121 993 685 586 191 176 42 21
SfoI GGC'GCC
Cuts at: 203 236 272 280 423 720 1247 1495 203
Size: 33 36 8 143 297 527 248 2523
Fragments arranged by size:
2523 527 297 248 143 36 33 8
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3815
SgrAI Cr'CCGG_yG
Cuts at: 398 398
Size: 3815
SmaI CCC'GGG
Cuts at: 285 347 837 285
Size: 62 490 3263
Fragments arranged by size:
3263 490 62
SmlI C'TyrA_G
Cuts at: 754 970 1885 2377 2639 754
Size: 216 915 492 262 1930
Fragments arranged by size:
1930 915 492 262 216
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3815
SphI G_CATG'C
Cuts at: 868 1368 868
Size: 500 3315
SrfI GCCC'GGGC
Cuts at: 347 347
Size: 3815
StuI AGG'CCT
Cuts at: 250 250
Size: 3815
StyI C'CwwG_G
Cuts at: 134 949 1303 134
Size: 815 354 2646
Fragments arranged by size:
2646 815 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1398 1531 1990 2199 1398
Size: 133 459 209 3014
Fragments arranged by size:
3014 459 209 133
TatI w'GTAC_w
Cuts at: 3330 3330
Size: 3815
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 3783
Tth111I GACn'n_nGTC
Cuts at: 1612 1612
Size: 3815
XbaI T'CTAG_A
Cuts at: 846 846
Size: 3815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1310 1310
Size: 3815
XmaI C'CCGG_G
Cuts at: 283 345 835 283
Size: 62 490 3263
Fragments arranged by size:
3263 490 62
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SnaBI SspI SwaI XhoI XmnI ZraI