Restriction map for
pF1KB8772
(Circular) (Six-base) MAPSORT of: pF1KB8772.seq Check: 9101 from: 1 to: 4787
pF1KB8772 4787 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1825 1825
Size: 4787
Acc65I G'GTAC_C
Cuts at: 1803 1803
Size: 4787
AclI AA'CG_TT
Cuts at: 4627 4627
Size: 4787
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 692 2612 3044 3791 4059 494
Size: 198 1920 432 747 268 1222
Fragments arranged by size:
1920 1222 747 432 268 198
AflII C'TTAA_G
Cuts at: 1063 1063
Size: 4787
AflIII A'CryG_T
Cuts at: 2139 3243 4220 2139
Size: 1104 977 2706
Fragments arranged by size:
2706 1104 977
AgeI A'CCGG_T
Cuts at: 3175 3175
Size: 4787
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 430 430
Size: 4787
AlwNI CAG_nnn'CTG
Cuts at: 226 2092 3659 226
Size: 1866 1567 1354
Fragments arranged by size:
1866 1567 1354
ApaLI G'TGCA_C
Cuts at: 3557 3557
Size: 4787
ApoI r'AATT_y
Cuts at: 1375 1611 1791 4140 4396 1375
Size: 236 180 2349 256 1766
Fragments arranged by size:
2349 1766 256 236 180
AseI AT'TA_AT
Cuts at: 20 1768 20
Size: 1748 3039
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
AvaI C'yCGr_G
Cuts at: 1807 1807
Size: 4787
BamHI G'GATC_C
Cuts at: 1077 1812 1077
Size: 735 4052
BanI G'GyrC_C
Cuts at: 1803 2217 2465 2500 1803
Size: 414 248 35 4090
Fragments arranged by size:
4090 414 248 35
BanII G_rGCy'C
Cuts at: 1801 2831 1801
Size: 1030 3757
BbeI G_GCGC'C
Cuts at: 2221 2469 2221
Size: 248 4539
BbsI GAAGACnn'nnnn_
Cuts at: 1244 1244
Size: 4787
BbvCI CC'TCA_GC
Cuts at: 149 227 149
Size: 78 4709
BciVI GTATCCnnnnn_n'
Cuts at: 2685 3446 4787 2685
Size: 761 1341 2685
Fragments arranged by size:
2685 1341 761
BclI T'GATC_A
Cuts at: 338 1514 2307 338
Size: 1176 793 2818
Fragments arranged by size:
2818 1176 793
BglII A'GATC_T
Cuts at: 2302 4339 2302
Size: 2037 2750
BlpI GC'TnA_GC
Cuts at: 1899 1899
Size: 4787
Bme1580I G_kGCm'C
Cuts at: 1364 2412 2505 3561 1364
Size: 1048 93 1056 2590
Fragments arranged by size:
2590 1056 1048 93
BmrI ACTGGGnnnn_n'
Cuts at: 554 1999 2080 2415 4559 554
Size: 1445 81 335 2144 782
Fragments arranged by size:
2144 1445 782 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3154 3154
Size: 4787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 656 821 1238 656
Size: 165 417 4205
Fragments arranged by size:
4205 417 165
Bpu10I CC'TnA_GC
Cuts at: 149 227 149
Size: 78 4709
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1124 1386 1963 2842 3334 3632 1124
Size: 262 577 879 492 298 2279
Fragments arranged by size:
2279 879 577 492 298 262
BsaI GGTCTCn'nnnn_
Cuts at: 565 673 856 4491 565
Size: 108 183 3635 861
Fragments arranged by size:
3635 861 183 108
BsaAI yAC'GTr
Cuts at: 1015 2140 1015
Size: 1125 3662
BsaBI GATnn'nnATC
Cuts at: 6 2301 4338 6
Size: 2295 2037 455
Fragments arranged by size:
2295 2037 455
BsaHI Gr'CG_yC
Cuts at: 2218 2466 3168 4664 2218
Size: 248 702 1496 2341
Fragments arranged by size:
2341 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1976 2497 3175 3449 3596 4007 1976
Size: 521 678 274 147 411 2756
Fragments arranged by size:
2756 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 869 869
Size: 4787
BseYI C'CCAG_C
Cuts at: 2212 2970 3547 4043 2212
Size: 758 577 496 2956
Fragments arranged by size:
2956 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1354 1354
Size: 4787
BsiEI CG_ry'CG
Cuts at: 104 2329 2375 3583 104
Size: 2225 46 1208 1308
Fragments arranged by size:
2225 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1733 1801 2579 2769 3561 1733
Size: 68 778 190 792 2959
Fragments arranged by size:
2959 792 778 190 68
Bsp1286I G_dGCh'C
Cuts at: 1364 1733 1801 2412 2505 2579 2769 2831 3561
Size: 369 68 611 93 74 190 62 730
Cuts at: 3561 1364
Size: 2590
Fragments arranged by size:
2590 730 611 369 190 93 74 68
62
BspEI T'CCGG_A
Cuts at: 4007 4007
Size: 4787
BspHI T'CATG_A
Cuts at: 1089 1152 3963 1089
Size: 63 2811 1913
Fragments arranged by size:
2811 1913 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1837 2051 2353 2734 1837
Size: 214 302 381 3890
Fragments arranged by size:
3890 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3079 3133 4617 4782 50
Size: 3029 54 1484 165 55
Fragments arranged by size:
3029 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 287 2699 287
Size: 2412 2375
BsrFI r'CCGG_y
Cuts at: 2785 2966 3175 2785
Size: 181 209 4397
Fragments arranged by size:
4397 209 181
BssSI C'ACGA_G
Cuts at: 3058 3416 3058
Size: 358 4429
BstAPI GCAn_nnn'nTGC
Cuts at: 831 2164 831
Size: 1333 3454
BstBI TT'CG_AA
Cuts at: 3148 3148
Size: 4787
BstYI r'GATC_y
Cuts at: 1 713 905 1077 1812 2279 2302 2637 2883
Size: 712 192 172 735 467 23 335 246
Cuts at: 2883 3895 3981 3993 4339 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 735 712 467 449 346 335 246
192 172 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4787
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2737 2918 3081 2737
Size: 181 163 4443
Fragments arranged by size:
4443 181 163
BtsI GCAGTG_nn'
Cuts at: 185 227 1002 2030 185
Size: 42 775 1028 2942
Fragments arranged by size:
2942 1028 775 42
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 996 1031 996
Size: 35 4752
DraI TTT'AAA
Cuts at: 1466 1786 1466
Size: 320 4467
DraIII CAC_nnn'GTG
Cuts at: 1392 1392
Size: 4787
DrdI GACnn_nn'nnGTC
Cuts at: 2493 3351 2493
Size: 858 3929
EaeI y'GGCC_r
Cuts at: 2372 2546 2937 2964 4309 2372
Size: 174 391 27 1345 2850
Fragments arranged by size:
2850 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2372 2372
Size: 4787
EarI CTCTTCn'nnn_
Cuts at: 563 635 671 692 2810 3020 563
Size: 72 36 21 2118 210 2330
Fragments arranged by size:
2330 2118 210 72 36 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2995 3305 3451 4597 2995
Size: 310 146 1146 3185
Fragments arranged by size:
3185 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1799 1799
Size: 4787
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 656 692 821 1238 2612 3044 3791 4059
Size: 162 36 129 417 1374 432 747 268
Cuts at: 4059 494
Size: 1222
Fragments arranged by size:
1374 1222 747 432 417 268 162 129
36
EcoNI CCTnn'n_nnAGG
Cuts at: 650 839 650
Size: 189 4598
EcoO109I rG'GnC_Cy
Cuts at: 512 656 1926 512
Size: 144 1270 3373
Fragments arranged by size:
3373 1270 144
EcoRI G'AATT_C
Cuts at: 1791 1791
Size: 4787
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2806 2806
Size: 4787
FspI TGC'GCA
Cuts at: 2568 2568
Size: 4787
HaeII r_GCGC'y
Cuts at: 2221 2469 3491 4470 2221
Size: 248 1022 979 2538
Fragments arranged by size:
2538 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 861 893 1228 1260 1499 1531 1742 1774 2629
Size: 32 335 32 239 32 211 32 855
Cuts at: 2629 2659 2661 2691 861
Size: 30 2 30 2957
Fragments arranged by size:
2957 855 335 239 211 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 114 1070 1826 114
Size: 956 756 3075
Fragments arranged by size:
3075 956 756
HpaI GTT'AAC
Cuts at: 1070 1070
Size: 4787
KasI G'GCGC_C
Cuts at: 2217 2465 2217
Size: 248 4539
KpnI G_GTAC'C
Cuts at: 1807 1807
Size: 4787
MluI A'CGCG_T
Cuts at: 4220 4220
Size: 4787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3458 3642 3458
Size: 184 4603
MscI TGG'CCA
Cuts at: 2548 2548
Size: 4787
MslI CAynn'nnrTG
Cuts at: 442 583 2776 2903 442
Size: 141 2193 127 2326
Fragments arranged by size:
2326 2193 141 127
MspA1I CmG'CkG
Cuts at: 1338 1898 2212 2572 3585 3830 1338
Size: 560 314 360 1013 245 2295
Fragments arranged by size:
2295 1013 560 360 314 245
NaeI GCC'GGC
Cuts at: 2968 2968
Size: 4787
NarI GG'CG_CC
Cuts at: 2218 2466 2218
Size: 248 4539
NcoI C'CATG_G
Cuts at: 107 107
Size: 4787
NgoMIV G'CCGG_C
Cuts at: 2966 2966
Size: 4787
NspI r_CATG'y
Cuts at: 1840 2340 3247 1840
Size: 500 907 3380
Fragments arranged by size:
3380 907 500
PciI A'CATG_T
Cuts at: 3243 3243
Size: 4787
PfoI T'CCnGG_A
Cuts at: 716 1685 716
Size: 969 3818
PmeI GTTT'AAAC
Cuts at: 1786 1786
Size: 4787
PmlI CAC'GTG
Cuts at: 1015 1015
Size: 4787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 3926
Size: 32 4755
PpuMI rG'GwC_Cy
Cuts at: 512 656 512
Size: 144 4643
PshAI GACnn'nnGTC
Cuts at: 367 367
Size: 4787
PsiI TTA'TAA
Cuts at: 698 698
Size: 4787
PstI C_TGCA'G
Cuts at: 1834 2519 1834
Size: 685 4102
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4787
PvuII CAG'CTG
Cuts at: 1338 2212 2572 1338
Size: 874 360 3553
Fragments arranged by size:
3553 874 360
RsrII CG'GwC_CG
Cuts at: 2982 2982
Size: 4787
SacI G_AGCT'C
Cuts at: 1801 1801
Size: 4787
SalI G'TCGA_C
Cuts at: 1824 1824
Size: 4787
SapI GCTCTTCn'nnn_
Cuts at: 635 2810 3020 635
Size: 2175 210 2402
Fragments arranged by size:
2402 2175 210
SbfI CC_TGCA'GG
Cuts at: 1834 1834
Size: 4787
ScaI AGT'ACT
Cuts at: 459 1529 4304 459
Size: 1070 2775 942
Fragments arranged by size:
2775 1070 942
SfcI C'TryA_G
Cuts at: 33 306 1830 2515 3508 3699 33
Size: 273 1524 685 993 191 1121
Fragments arranged by size:
1524 1121 993 685 273 191
SfoI GGC'GCC
Cuts at: 2219 2467 2219
Size: 248 4539
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
SmaI CCC'GGG
Cuts at: 1809 1809
Size: 4787
SmlI C'TyrA_G
Cuts at: 1063 1139 1365 1942 2857 3349 3611 1063
Size: 76 226 577 915 492 262 2239
Fragments arranged by size:
2239 915 577 492 262 226 76
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4787
SphI G_CATG'C
Cuts at: 1840 2340 1840
Size: 500 4287
SspI AAT'ATT
Cuts at: 507 1578 1587 1598 1682 507
Size: 1071 9 11 84 3612
Fragments arranged by size:
3612 1071 84 11 9
StuI AGG'CCT
Cuts at: 997 997
Size: 4787
StyI C'CwwG_G
Cuts at: 107 1646 1921 2275 107
Size: 1539 275 354 2619
Fragments arranged by size:
2619 1539 354 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2370 2503 2962 3171 2370
Size: 133 459 209 3986
Fragments arranged by size:
3986 459 209 133
TatI w'GTAC_w
Cuts at: 457 1527 4302 457
Size: 1070 2775 942
Fragments arranged by size:
2775 1070 942
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4304 4336 4304
Size: 32 4755
Tth111I GACn'n_nGTC
Cuts at: 2584 2584
Size: 4787
XbaI T'CTAG_A
Cuts at: 1818 1818
Size: 4787
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 265 845 2282 265
Size: 580 1437 2770
Fragments arranged by size:
2770 1437 580
XmaI C'CCGG_G
Cuts at: 1807 1807
Size: 4787
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI EcoRV FseI FspAI HindIII MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI