Restriction map for
pF1KB8775
(Circular) (Six-base) MAPSORT of: pF1KB8775.seq Check: 5049 from: 1 to: 3854
pF1KB8775 3854 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 892 892
Size: 3854
Acc65I G'GTAC_C
Cuts at: 870 870
Size: 3854
AclI AA'CG_TT
Cuts at: 3694 3694
Size: 3854
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 672 1679 2111 2858 3126 146
Size: 526 1007 432 747 268 874
Fragments arranged by size:
1007 874 747 526 432 268
AflIII A'CryG_T
Cuts at: 476 1206 2310 3287 476
Size: 730 1104 977 1043
Fragments arranged by size:
1104 1043 977 730
AgeI A'CCGG_T
Cuts at: 2242 2242
Size: 3854
AleI CACnn'nnGTG
Cuts at: 550 595 550
Size: 45 3809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 649 649
Size: 3854
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 613 645 613
Size: 32 3822
AlwNI CAG_nnn'CTG
Cuts at: 1159 2726 1159
Size: 1567 2287
ApaLI G'TGCA_C
Cuts at: 2624 2624
Size: 3854
ApoI r'AATT_y
Cuts at: 559 820 858 3207 3463 559
Size: 261 38 2349 256 950
Fragments arranged by size:
2349 950 261 256 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 3854
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3854
AvaI C'yCGr_G
Cuts at: 874 874
Size: 3854
BamHI G'GATC_C
Cuts at: 879 879
Size: 3854
BanI G'GyrC_C
Cuts at: 837 870 1284 1532 1567 837
Size: 33 414 248 35 3124
Fragments arranged by size:
3124 414 248 35 33
BanII G_rGCy'C
Cuts at: 529 544 868 1898 529
Size: 15 324 1030 2485
Fragments arranged by size:
2485 1030 324 15
BbeI G_GCGC'C
Cuts at: 1288 1536 1288
Size: 248 3606
BbsI GAAGACnn'nnnn_
Cuts at: 134 134
Size: 3854
BciVI GTATCCnnnnn_n'
Cuts at: 1752 2513 3854 1752
Size: 761 1341 1752
Fragments arranged by size:
1752 1341 761
BclI T'GATC_A
Cuts at: 1374 1374
Size: 3854
BglII A'GATC_T
Cuts at: 1369 3406 1369
Size: 2037 1817
BlpI GC'TnA_GC
Cuts at: 966 966
Size: 3854
Bme1580I G_kGCm'C
Cuts at: 1479 1572 2628 1479
Size: 93 1056 2705
Fragments arranged by size:
2705 1056 93
BmgBI CAC'GTC
Cuts at: 320 479 629 320
Size: 159 150 3545
Fragments arranged by size:
3545 159 150
BmrI ACTGGGnnnn_n'
Cuts at: 1066 1147 1482 3626 1066
Size: 81 335 2144 1294
Fragments arranged by size:
2144 1294 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 563 2221 563
Size: 1658 2196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 238 181
Size: 57 3797
Bpu10I CC'TnA_GC
Cuts at: 538 538
Size: 3854
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1909 2401 2699 1030
Size: 879 492 298 2185
Fragments arranged by size:
2185 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3558 3558
Size: 3854
BsaAI yAC'GTr
Cuts at: 1207 1207
Size: 3854
BsaBI GATnn'nnATC
Cuts at: 6 1368 3405 6
Size: 1362 2037 455
Fragments arranged by size:
2037 1362 455
BsaHI Gr'CG_yC
Cuts at: 1285 1533 2235 3731 1285
Size: 248 702 1496 1408
Fragments arranged by size:
1496 1408 702 248
BsaWI w'CCGG_w
Cuts at: 1043 1564 2242 2516 2663 3074 1043
Size: 521 678 274 147 411 1823
Fragments arranged by size:
1823 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 466 496 676 706 466
Size: 30 180 30 3614
Fragments arranged by size:
3614 180 30 30
BseYI C'CCAG_C
Cuts at: 1279 2037 2614 3110 1279
Size: 758 577 496 2023
Fragments arranged by size:
2023 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 609 535
Size: 74 3780
BsiEI CG_ry'CG
Cuts at: 104 1396 1442 2650 104
Size: 1292 46 1208 1308
Fragments arranged by size:
1308 1292 1208 46
BsiHKAI G_wGCw'C
Cuts at: 529 544 868 1646 1836 2628 529
Size: 15 324 778 190 792 1755
Fragments arranged by size:
1755 792 778 324 190 15
BsmBI CGTCTCn'nnnn_
Cuts at: 310 310
Size: 3854
Bsp1286I G_dGCh'C
Cuts at: 529 544 868 1479 1572 1646 1836 1898 2628
Size: 15 324 611 93 74 190 62 730
Cuts at: 2628 529
Size: 1755
Fragments arranged by size:
1755 730 611 324 190 93 74 62
15
BspEI T'CCGG_A
Cuts at: 3074 3074
Size: 3854
BspHI T'CATG_A
Cuts at: 816 3030 816
Size: 2214 1640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 299 904 1118 1420 1801 299
Size: 605 214 302 381 2352
Fragments arranged by size:
2352 605 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2146 2200 3684 3849 50
Size: 2096 54 1484 165 55
Fragments arranged by size:
2096 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1766 1766
Size: 3854
BsrFI r'CCGG_y
Cuts at: 1852 2033 2242 1852
Size: 181 209 3464
Fragments arranged by size:
3464 209 181
BssSI C'ACGA_G
Cuts at: 2125 2483 2125
Size: 358 3496
BstAPI GCAn_nnn'nTGC
Cuts at: 1231 1231
Size: 3854
BstBI TT'CG_AA
Cuts at: 2215 2215
Size: 3854
BstXI CCAn_nnnn'nTGG
Cuts at: 216 450 492 552 216
Size: 234 42 60 3518
Fragments arranged by size:
3518 234 60 42
BstYI r'GATC_y
Cuts at: 1 879 1346 1369 1704 1950 2962 3048 3060
Size: 878 467 23 335 246 1012 86 12
Cuts at: 3060 3406 1
Size: 346 449
Fragments arranged by size:
1012 878 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 702 702
Size: 3854
BtgI C'CryG_G
Cuts at: 107 107
Size: 3854
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1804 1985 2148 1804
Size: 181 163 3510
Fragments arranged by size:
3510 181 163
BtsI GCAGTG_nn'
Cuts at: 131 1097 131
Size: 966 2888
ClaI AT'CG_AT
Cuts at: 183 183
Size: 3854
DraI TTT'AAA
Cuts at: 853 853
Size: 3854
DraIII CAC_nnn'GTG
Cuts at: 286 286
Size: 3854
DrdI GACnn_nn'nnGTC
Cuts at: 1560 2418 1560
Size: 858 2996
EaeI y'GGCC_r
Cuts at: 110 1439 1613 2004 2031 3376 110
Size: 1329 174 391 27 1345 588
Fragments arranged by size:
1345 1329 588 391 174 27
EagI C'GGCC_G
Cuts at: 1439 1439
Size: 3854
EarI CTCTTCn'nnn_
Cuts at: 1877 2087 1877
Size: 210 3644
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2062 2372 2518 3664 2062
Size: 310 146 1146 2252
Fragments arranged by size:
2252 1146 310 146
EcoICRI GAG'CTC
Cuts at: 527 542 866 527
Size: 15 324 3515
Fragments arranged by size:
3515 324 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 181 238 672 1679 2111 2858 3126 146
Size: 35 57 434 1007 432 747 268 874
Fragments arranged by size:
1007 874 747 434 432 268 57 35
EcoO109I rG'GnC_Cy
Cuts at: 993 993
Size: 3854
EcoRI G'AATT_C
Cuts at: 858 858
Size: 3854
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 754 1873 754
Size: 1119 2735
FspI TGC'GCA
Cuts at: 1635 1635
Size: 3854
HaeII r_GCGC'y
Cuts at: 1288 1536 2558 3537 1288
Size: 248 1022 979 1605
Fragments arranged by size:
1605 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1696 1726 1728 1758 1696
Size: 30 2 30 3792
Fragments arranged by size:
3792 30 30 2
HincII GTy'rAC
Cuts at: 118 893 118
Size: 775 3079
KasI G'GCGC_C
Cuts at: 1284 1532 1284
Size: 248 3606
KpnI G_GTAC'C
Cuts at: 874 874
Size: 3854
MluI A'CGCG_T
Cuts at: 3287 3287
Size: 3854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 509 2525 2709 509
Size: 2016 184 1654
Fragments arranged by size:
2016 1654 184
MscI TGG'CCA
Cuts at: 112 1615 112
Size: 1503 2351
MslI CAynn'nnrTG
Cuts at: 511 550 595 655 1843 1970 511
Size: 39 45 60 1188 127 2395
Fragments arranged by size:
2395 1188 127 60 45 39
MspA1I CmG'CkG
Cuts at: 965 1279 1639 2652 2897 965
Size: 314 360 1013 245 1922
Fragments arranged by size:
1922 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2035 2035
Size: 3854
NarI GG'CG_CC
Cuts at: 1285 1533 1285
Size: 248 3606
NcoI C'CATG_G
Cuts at: 107 107
Size: 3854
NgoMIV G'CCGG_C
Cuts at: 2033 2033
Size: 3854
NspI r_CATG'y
Cuts at: 907 1407 2314 907
Size: 500 907 2447
Fragments arranged by size:
2447 907 500
PciI A'CATG_T
Cuts at: 2310 2310
Size: 3854
PmeI GTTT'AAAC
Cuts at: 853 853
Size: 3854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2993 3025 2993
Size: 32 3822
PstI C_TGCA'G
Cuts at: 310 901 1586 310
Size: 591 685 2578
Fragments arranged by size:
2578 685 591
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3854
PvuII CAG'CTG
Cuts at: 1279 1639 1279
Size: 360 3494
RsrII CG'GwC_CG
Cuts at: 2049 2049
Size: 3854
SacI G_AGCT'C
Cuts at: 529 544 868 529
Size: 15 324 3515
Fragments arranged by size:
3515 324 15
SalI G'TCGA_C
Cuts at: 891 891
Size: 3854
SapI GCTCTTCn'nnn_
Cuts at: 1877 2087 1877
Size: 210 3644
SbfI CC_TGCA'GG
Cuts at: 901 901
Size: 3854
ScaI AGT'ACT
Cuts at: 3371 3371
Size: 3854
SfcI C'TryA_G
Cuts at: 33 306 897 1582 2575 2766 33
Size: 273 591 685 993 191 1121
Fragments arranged by size:
1121 993 685 591 273 191
SfoI GGC'GCC
Cuts at: 1286 1534 1286
Size: 248 3606
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3854
SmaI CCC'GGG
Cuts at: 876 876
Size: 3854
SmlI C'TyrA_G
Cuts at: 1009 1924 2416 2678 1009
Size: 915 492 262 2185
Fragments arranged by size:
2185 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3854
SphI G_CATG'C
Cuts at: 907 1407 907
Size: 500 3354
StuI AGG'CCT
Cuts at: 148 570 148
Size: 422 3432
StyI C'CwwG_G
Cuts at: 107 738 988 1342 107
Size: 631 250 354 2619
Fragments arranged by size:
2619 631 354 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1437 1570 2029 2238 1437
Size: 133 459 209 3053
Fragments arranged by size:
3053 459 209 133
TatI w'GTAC_w
Cuts at: 3369 3369
Size: 3854
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 466 498 3371 3403 466
Size: 32 2873 32 917
Fragments arranged by size:
2873 917 32 32
Tth111I GACn'n_nGTC
Cuts at: 586 1651 586
Size: 1065 2789
XbaI T'CTAG_A
Cuts at: 825 885 825
Size: 60 3794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1349 1349
Size: 3854
XmaI C'CCGG_G
Cuts at: 874 874
Size: 3854
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI BseRI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI