Restriction map for
pF1KB8776
(Circular) (Six-base) MAPSORT of: pF1KB8776.seq Check: 1036 from: 1 to: 4736
pF1KB8776 4736 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1774 1774
Size: 4736
Acc65I G'GTAC_C
Cuts at: 1752 1752
Size: 4736
AclI AA'CG_TT
Cuts at: 4576 4576
Size: 4736
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1482 2561 2993 3740 4008 1482
Size: 1079 432 747 268 2210
Fragments arranged by size:
2210 1079 747 432 268
AflIII A'CryG_T
Cuts at: 2088 3192 4169 2088
Size: 1104 977 2655
Fragments arranged by size:
2655 1104 977
AgeI A'CCGG_T
Cuts at: 1120 3124 1120
Size: 2004 2732
AleI CACnn'nnGTG
Cuts at: 1121 1121
Size: 4736
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 344 344
Size: 4736
AlwNI CAG_nnn'CTG
Cuts at: 1511 2041 3608 1511
Size: 530 1567 2639
Fragments arranged by size:
2639 1567 530
ApaLI G'TGCA_C
Cuts at: 3506 3506
Size: 4736
ApoI r'AATT_y
Cuts at: 132 642 944 1002 1532 1740 4089 4345 132
Size: 510 302 58 530 208 2349 256 523
Fragments arranged by size:
2349 530 523 510 302 256 208 58
AseI AT'TA_AT
Cuts at: 20 20
Size: 4736
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4736
AvaI C'yCGr_G
Cuts at: 1756 1756
Size: 4736
BamHI G'GATC_C
Cuts at: 1761 1761
Size: 4736
BanI G'GyrC_C
Cuts at: 1439 1752 2166 2414 2449 1439
Size: 313 414 248 35 3726
Fragments arranged by size:
3726 414 313 248 35
BanII G_rGCy'C
Cuts at: 670 1678 1750 2780 670
Size: 1008 72 1030 2626
Fragments arranged by size:
2626 1030 1008 72
BbeI G_GCGC'C
Cuts at: 2170 2418 2170
Size: 248 4488
BciVI GTATCCnnnnn_n'
Cuts at: 2634 3395 4736 2634
Size: 761 1341 2634
Fragments arranged by size:
2634 1341 761
BclI T'GATC_A
Cuts at: 2256 2256
Size: 4736
BglII A'GATC_T
Cuts at: 2251 4288 2251
Size: 2037 2699
BlpI GC'TnA_GC
Cuts at: 921 1848 921
Size: 927 3809
Bme1580I G_kGCm'C
Cuts at: 2361 2454 3510 2361
Size: 93 1056 3587
Fragments arranged by size:
3587 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1948 2029 2364 4508 1948
Size: 81 335 2144 2176
Fragments arranged by size:
2176 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3103 3103
Size: 4736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 722 1226 289
Size: 433 504 3799
Fragments arranged by size:
3799 504 433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 1912 2791 3283 3581 267
Size: 1645 879 492 298 1422
Fragments arranged by size:
1645 1422 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1540 4440 1540
Size: 2900 1836
BsaAI yAC'GTr
Cuts at: 2089 2089
Size: 4736
BsaBI GATnn'nnATC
Cuts at: 6 2250 4287 6
Size: 2244 2037 455
Fragments arranged by size:
2244 2037 455
BsaHI Gr'CG_yC
Cuts at: 2167 2415 3117 4613 2167
Size: 248 702 1496 2290
Fragments arranged by size:
2290 1496 702 248
BsaWI w'CCGG_w
Cuts at: 784 1120 1925 2446 3124 3398 3545 3956 784
Size: 336 805 521 678 274 147 411 1564
Fragments arranged by size:
1564 805 678 521 411 336 274 147
BseYI C'CCAG_C
Cuts at: 430 1184 1636 2161 2919 3496 3992 430
Size: 754 452 525 758 577 496 1174
Fragments arranged by size:
1174 758 754 577 525 496 452
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1247 1247
Size: 4736
BsiEI CG_ry'CG
Cuts at: 104 2278 2324 3532 104
Size: 2174 46 1208 1308
Fragments arranged by size:
2174 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 670 1678 1704 1750 2528 2718 3510 670
Size: 1008 26 46 778 190 792 1896
Fragments arranged by size:
1896 1008 792 778 190 46 26
BsmI GAATG_Cn'
Cuts at: 892 976 892
Size: 84 4652
BsmBI CGTCTCn'nnnn_
Cuts at: 356 356
Size: 4736
Bsp1286I G_dGCh'C
Cuts at: 670 1678 1704 1750 2361 2454 2528 2718 2780
Size: 1008 26 46 611 93 74 190 62
Cuts at: 2780 3510 670
Size: 730 1896
Fragments arranged by size:
1896 1008 730 611 190 93 74 62
46 26
BspEI T'CCGG_A
Cuts at: 3956 3956
Size: 4736
BspHI T'CATG_A
Cuts at: 3912 3912
Size: 4736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1786 2000 2302 2683 1786
Size: 214 302 381 3839
Fragments arranged by size:
3839 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3028 3082 4566 4731 50
Size: 2978 54 1484 165 55
Fragments arranged by size:
2978 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2648 2648
Size: 4736
BsrFI r'CCGG_y
Cuts at: 1120 1640 2734 2915 3124 1120
Size: 520 1094 181 209 2732
Fragments arranged by size:
2732 1094 520 209 181
BssSI C'ACGA_G
Cuts at: 3007 3365 3007
Size: 358 4378
BstAPI GCAn_nnn'nTGC
Cuts at: 2113 2113
Size: 4736
BstBI TT'CG_AA
Cuts at: 3097 3097
Size: 4736
BstYI r'GATC_y
Cuts at: 1 1280 1761 2228 2251 2586 2832 3844 3930
Size: 1279 481 467 23 335 246 1012 86
Cuts at: 3930 3942 4288 1
Size: 12 346 449
Fragments arranged by size:
1279 1012 481 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 503 1721 503
Size: 1218 3518
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 656 2686 2867 3030 656
Size: 2030 181 163 2362
Fragments arranged by size:
2362 2030 181 163
BtsI GCAGTG_nn'
Cuts at: 1979 1979
Size: 4736
DraI TTT'AAA
Cuts at: 1735 1735
Size: 4736
DrdI GACnn_nn'nnGTC
Cuts at: 2442 3300 2442
Size: 858 3878
EaeI y'GGCC_r
Cuts at: 2321 2495 2886 2913 4258 2321
Size: 174 391 27 1345 2799
Fragments arranged by size:
2799 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2321 2321
Size: 4736
EarI CTCTTCn'nnn_
Cuts at: 759 2759 2969 759
Size: 2000 210 2526
Fragments arranged by size:
2526 2000 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2944 3254 3400 4546 2944
Size: 310 146 1146 3134
Fragments arranged by size:
3134 1146 310 146
EcoICRI GAG'CTC
Cuts at: 668 1676 1748 668
Size: 1008 72 3656
Fragments arranged by size:
3656 1008 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 722 1226 1482 2561 2993 3740 4008 289
Size: 433 504 256 1079 432 747 268 1017
Fragments arranged by size:
1079 1017 747 504 433 432 268 256
EcoO109I rG'GnC_Cy
Cuts at: 1633 1875 1633
Size: 242 4494
EcoRI G'AATT_C
Cuts at: 944 1532 1740 944
Size: 588 208 3940
Fragments arranged by size:
3940 588 208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 4736
FspI TGC'GCA
Cuts at: 2517 2517
Size: 4736
HaeII r_GCGC'y
Cuts at: 2170 2418 3440 4419 2170
Size: 248 1022 979 2487
Fragments arranged by size:
2487 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 837 869 1539 1571 2578 2608 2610 2640 837
Size: 32 670 32 1007 30 2 30 2933
Fragments arranged by size:
2933 1007 670 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1775 1775
Size: 4736
HindIII A'AGCT_T
Cuts at: 905 905
Size: 4736
KasI G'GCGC_C
Cuts at: 2166 2414 2166
Size: 248 4488
KpnI G_GTAC'C
Cuts at: 1756 1756
Size: 4736
MluI A'CGCG_T
Cuts at: 4169 4169
Size: 4736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3407 3591 3407
Size: 184 4552
MscI TGG'CCA
Cuts at: 2497 2497
Size: 4736
MslI CAynn'nnrTG
Cuts at: 1053 1121 1457 1557 2725 2852 1053
Size: 68 336 100 1168 127 2937
Fragments arranged by size:
2937 1168 336 127 100 68
MspA1I CmG'CkG
Cuts at: 1847 2161 2521 3534 3779 1847
Size: 314 360 1013 245 2804
Fragments arranged by size:
2804 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2917 2917
Size: 4736
NarI GG'CG_CC
Cuts at: 2167 2415 2167
Size: 248 4488
NcoI C'CATG_G
Cuts at: 503 1721 503
Size: 1218 3518
NgoMIV G'CCGG_C
Cuts at: 2915 2915
Size: 4736
NspI r_CATG'y
Cuts at: 1789 2289 3196 1789
Size: 500 907 3329
Fragments arranged by size:
3329 907 500
PciI A'CATG_T
Cuts at: 3192 3192
Size: 4736
PflMI CCAn_nnn'nTGG
Cuts at: 504 1570 504
Size: 1066 3670
PfoI T'CCnGG_A
Cuts at: 1163 1163
Size: 4736
PmeI GTTT'AAAC
Cuts at: 1735 1735
Size: 4736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 3875
Size: 32 4704
PpuMI rG'GwC_Cy
Cuts at: 1633 1633
Size: 4736
PstI C_TGCA'G
Cuts at: 1783 2468 1783
Size: 685 4051
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4736
PvuII CAG'CTG
Cuts at: 2161 2521 2161
Size: 360 4376
RsrII CG'GwC_CG
Cuts at: 2931 2931
Size: 4736
SacI G_AGCT'C
Cuts at: 670 1678 1750 670
Size: 1008 72 3656
Fragments arranged by size:
3656 1008 72
SalI G'TCGA_C
Cuts at: 1773 1773
Size: 4736
SanDI GG'GwC_CC
Cuts at: 1633 1633
Size: 4736
SapI GCTCTTCn'nnn_
Cuts at: 2759 2969 2759
Size: 210 4526
SbfI CC_TGCA'GG
Cuts at: 1783 1783
Size: 4736
ScaI AGT'ACT
Cuts at: 4253 4253
Size: 4736
SfcI C'TryA_G
Cuts at: 33 1779 2464 3457 3648 33
Size: 1746 685 993 191 1121
Fragments arranged by size:
1746 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2168 2416 2168
Size: 248 4488
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4736
SgrAI Cr'CCGG_yG
Cuts at: 1120 1120
Size: 4736
SmaI CCC'GGG
Cuts at: 1758 1758
Size: 4736
SmlI C'TyrA_G
Cuts at: 246 1891 2806 3298 3560 246
Size: 1645 915 492 262 1422
Fragments arranged by size:
1645 1422 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4736
SphI G_CATG'C
Cuts at: 1789 2289 1789
Size: 500 4236
StyI C'CwwG_G
Cuts at: 503 1721 1870 2224 503
Size: 1218 149 354 3015
Fragments arranged by size:
3015 1218 354 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1198 2319 2452 2911 3120 1198
Size: 1121 133 459 209 2814
Fragments arranged by size:
2814 1121 459 209 133
TatI w'GTAC_w
Cuts at: 721 1605 4251 721
Size: 884 2646 1206
Fragments arranged by size:
2646 1206 884
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4253 4285 4253
Size: 32 4704
Tth111I GACn'n_nGTC
Cuts at: 2533 2533
Size: 4736
XbaI T'CTAG_A
Cuts at: 1767 1767
Size: 4736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2231 2231
Size: 4736
XmaI C'CCGG_G
Cuts at: 1756 1756
Size: 4736
XmnI GAAnn'nnTTC
Cuts at: 512 907 1075 1243 1411 1579 512
Size: 395 168 168 168 168 3669
Fragments arranged by size:
3669 395 168 168 168 168
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SnaBI SrfI SspI StuI
SwaI XhoI ZraI