Restriction map for
pF1KB8777
(Circular) (Six-base) MAPSORT of: pF1KB8777.seq Check: 5721 from: 1 to: 3884
pF1KB8777 3884 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 374 374
Size: 3884
AccI GT'mk_AC
Cuts at: 922 922
Size: 3884
Acc65I G'GTAC_C
Cuts at: 900 900
Size: 3884
AclI AA'CG_TT
Cuts at: 3724 3724
Size: 3884
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 334 607 847 1709 2141 2888 3156 256
Size: 78 273 240 862 432 747 268 984
Fragments arranged by size:
984 862 747 432 273 268 240 78
AflIII A'CryG_T
Cuts at: 1236 2340 3317 1236
Size: 1104 977 1803
Fragments arranged by size:
1803 1104 977
AgeI A'CCGG_T
Cuts at: 2272 2272
Size: 3884
AleI CACnn'nnGTG
Cuts at: 631 718 631
Size: 87 3797
AlwNI CAG_nnn'CTG
Cuts at: 241 366 672 692 1189 2756 241
Size: 125 306 20 497 1567 1369
Fragments arranged by size:
1567 1369 497 306 125 20
ApaI G_GGCC'C
Cuts at: 878 878
Size: 3884
ApaLI G'TGCA_C
Cuts at: 2654 2654
Size: 3884
ApoI r'AATT_y
Cuts at: 888 3237 3493 888
Size: 2349 256 1279
Fragments arranged by size:
2349 1279 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3884
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
AvaI C'yCGr_G
Cuts at: 155 283 870 904 155
Size: 128 587 34 3135
Fragments arranged by size:
3135 587 128 34
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 517 550 517
Size: 33 3851
BamHI G'GATC_C
Cuts at: 488 909 488
Size: 421 3463
BanI G'GyrC_C
Cuts at: 112 196 221 682 812 900 1314 1562 1597
Size: 84 25 461 130 88 414 248 35
Cuts at: 1597 112
Size: 2399
Fragments arranged by size:
2399 461 414 248 130 88 84 35
25
BanII G_rGCy'C
Cuts at: 878 898 1928 878
Size: 20 1030 2834
Fragments arranged by size:
2834 1030 20
BbeI G_GCGC'C
Cuts at: 200 1318 1566 200
Size: 1118 248 2518
Fragments arranged by size:
2518 1118 248
BbsI GAAGACnn'nnnn_
Cuts at: 243 243
Size: 3884
BciVI GTATCCnnnnn_n'
Cuts at: 1782 2543 3884 1782
Size: 761 1341 1782
Fragments arranged by size:
1782 1341 761
BclI T'GATC_A
Cuts at: 1404 1404
Size: 3884
BglI GCCn_nnn'nGGC
Cuts at: 409 409
Size: 3884
BglII A'GATC_T
Cuts at: 332 1399 3436 332
Size: 1067 2037 780
Fragments arranged by size:
2037 1067 780
BlpI GC'TnA_GC
Cuts at: 996 996
Size: 3884
Bme1580I G_kGCm'C
Cuts at: 115 226 878 1509 1602 2658 115
Size: 111 652 631 93 1056 1341
Fragments arranged by size:
1341 1056 652 631 111 93
BmgBI CAC'GTC
Cuts at: 720 720
Size: 3884
BmrI ACTGGGnnnn_n'
Cuts at: 1096 1177 1512 3656 1096
Size: 81 335 2144 1324
Fragments arranged by size:
2144 1324 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 3884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 838
Size: 3884
Bpu10I CC'TnA_GC
Cuts at: 376 376
Size: 3884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1939 2431 2729 1060
Size: 879 492 298 2215
Fragments arranged by size:
2215 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 153 3588 153
Size: 3435 449
BsaAI yAC'GTr
Cuts at: 1237 1237
Size: 3884
BsaBI GATnn'nnATC
Cuts at: 6 1398 3435 6
Size: 1392 2037 455
Fragments arranged by size:
2037 1392 455
BsaHI Gr'CG_yC
Cuts at: 197 500 1315 1563 2265 3761 197
Size: 303 815 248 702 1496 320
Fragments arranged by size:
1496 815 702 320 303 248
BsaWI w'CCGG_w
Cuts at: 1073 1594 2272 2546 2693 3104 1073
Size: 521 678 274 147 411 1853
Fragments arranged by size:
1853 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 319 349 562 592 319
Size: 30 213 30 3611
Fragments arranged by size:
3611 213 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 655 166
Size: 489 3395
BseYI C'CCAG_C
Cuts at: 838 1309 2067 2644 3140 838
Size: 471 758 577 496 1582
Fragments arranged by size:
1582 758 577 496 471
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 352 532 655 172
Size: 180 180 123 3401
Fragments arranged by size:
3401 180 180 123
BsiEI CG_ry'CG
Cuts at: 104 1426 1472 2680 104
Size: 1322 46 1208 1308
Fragments arranged by size:
1322 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 311 898 1676 1866 2658 311
Size: 587 778 190 792 1537
Fragments arranged by size:
1537 792 778 587 190
Bsp1286I G_dGCh'C
Cuts at: 115 226 311 878 898 1509 1602 1676 1866
Size: 111 85 567 20 611 93 74 190
Cuts at: 1866 1928 2658 115
Size: 62 730 1341
Fragments arranged by size:
1341 730 611 567 190 111 93 85
74 62 20
BspEI T'CCGG_A
Cuts at: 3104 3104
Size: 3884
BspHI T'CATG_A
Cuts at: 3060 3060
Size: 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 374 934 1148 1450 1831 374
Size: 560 214 302 381 2427
Fragments arranged by size:
2427 560 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2176 2230 3714 3879 50
Size: 2126 54 1484 165 55
Fragments arranged by size:
2126 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1796 1796
Size: 3884
BsrFI r'CCGG_y
Cuts at: 218 371 1882 2063 2272 218
Size: 153 1511 181 209 1830
Fragments arranged by size:
1830 1511 209 181 153
BssSI C'ACGA_G
Cuts at: 2155 2513 2155
Size: 358 3526
BstAPI GCAn_nnn'nTGC
Cuts at: 1261 1261
Size: 3884
BstBI TT'CG_AA
Cuts at: 2245 2245
Size: 3884
BstEII G'GTnAC_C
Cuts at: 732 732
Size: 3884
BstXI CCAn_nnnn'nTGG
Cuts at: 720 720
Size: 3884
BstYI r'GATC_y
Cuts at: 1 332 387 488 909 1376 1399 1734 1980
Size: 331 55 101 421 467 23 335 246
Cuts at: 1980 2992 3078 3090 3436 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 421 346 335 331 246
101 86 55 23 12
BtgI C'CryG_G
Cuts at: 107 755 107
Size: 648 3236
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1834 2015 2178 1834
Size: 181 163 3540
Fragments arranged by size:
3540 181 163
BtsI GCAGTG_nn'
Cuts at: 549 1127 549
Size: 578 3306
DraI TTT'AAA
Cuts at: 883 883
Size: 3884
DrdI GACnn_nn'nnGTC
Cuts at: 1590 2448 1590
Size: 858 3026
EaeI y'GGCC_r
Cuts at: 296 440 722 1469 1643 2034 2061 3406 296
Size: 144 282 747 174 391 27 1345 774
Fragments arranged by size:
1345 774 747 391 282 174 144 27
EagI C'GGCC_G
Cuts at: 1469 1469
Size: 3884
EarI CTCTTCn'nnn_
Cuts at: 175 184 1907 2117 175
Size: 9 1723 210 1942
Fragments arranged by size:
1942 1723 210 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 411 2092 2402 2548 3694 411
Size: 1681 310 146 1146 601
Fragments arranged by size:
1681 1146 601 310 146
EcoICRI GAG'CTC
Cuts at: 896 896
Size: 3884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 334 607 838 847 1709 2141 2888 3156
Size: 78 273 231 9 862 432 747 268
Cuts at: 3156 256
Size: 984
Fragments arranged by size:
984 862 747 432 273 268 231 78
9
EcoNI CCTnn'n_nnAGG
Cuts at: 274 568 274
Size: 294 3590
EcoO109I rG'GnC_Cy
Cuts at: 267 279 353 447 573 858 874 1023 267
Size: 12 74 94 126 285 16 149 3128
Fragments arranged by size:
3128 285 149 126 94 74 16 12
EcoRI G'AATT_C
Cuts at: 888 888
Size: 3884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1903 1903
Size: 3884
FspI TGC'GCA
Cuts at: 1665 1665
Size: 3884
HaeII r_GCGC'y
Cuts at: 200 521 845 1318 1566 2588 3567 200
Size: 321 324 473 248 1022 979 517
Fragments arranged by size:
1022 979 517 473 324 321 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 586 618 1726 1756 1758 1788 586
Size: 32 1108 30 2 30 2682
Fragments arranged by size:
2682 1108 32 30 30 2
HincII GTy'rAC
Cuts at: 923 923
Size: 3884
KasI G'GCGC_C
Cuts at: 196 1314 1562 196
Size: 1118 248 2518
Fragments arranged by size:
2518 1118 248
KpnI G_GTAC'C
Cuts at: 904 904
Size: 3884
MluI A'CGCG_T
Cuts at: 3317 3317
Size: 3884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2555 2739 2555
Size: 184 3700
MscI TGG'CCA
Cuts at: 724 1645 724
Size: 921 2963
MslI CAynn'nnrTG
Cuts at: 631 718 1873 2000 631
Size: 87 1155 127 2515
Fragments arranged by size:
2515 1155 127 87
MspA1I CmG'CkG
Cuts at: 995 1309 1669 2682 2927 995
Size: 314 360 1013 245 1952
Fragments arranged by size:
1952 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2065 2065
Size: 3884
NarI GG'CG_CC
Cuts at: 197 1315 1563 197
Size: 1118 248 2518
Fragments arranged by size:
2518 1118 248
NcoI C'CATG_G
Cuts at: 107 755 107
Size: 648 3236
NgoMIV G'CCGG_C
Cuts at: 2063 2063
Size: 3884
NspI r_CATG'y
Cuts at: 937 1437 2344 937
Size: 500 907 2477
Fragments arranged by size:
2477 907 500
PasI CC'CwG_GG
Cuts at: 504 504
Size: 3884
PciI A'CATG_T
Cuts at: 2340 2340
Size: 3884
PfoI T'CCnGG_A
Cuts at: 344 344
Size: 3884
PmeI GTTT'AAAC
Cuts at: 883 883
Size: 3884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3023 3055 3023
Size: 32 3852
PpuMI rG'GwC_Cy
Cuts at: 279 858 279
Size: 579 3305
PspOMI G'GGCC_C
Cuts at: 874 874
Size: 3884
PstI C_TGCA'G
Cuts at: 259 569 697 931 1616 259
Size: 310 128 234 685 2527
Fragments arranged by size:
2527 685 310 234 128
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3884
PvuII CAG'CTG
Cuts at: 1309 1669 1309
Size: 360 3524
RsrII CG'GwC_CG
Cuts at: 2079 2079
Size: 3884
SacI G_AGCT'C
Cuts at: 898 898
Size: 3884
SalI G'TCGA_C
Cuts at: 921 921
Size: 3884
SapI GCTCTTCn'nnn_
Cuts at: 1907 2117 1907
Size: 210 3674
SbfI CC_TGCA'GG
Cuts at: 569 931 569
Size: 362 3522
ScaI AGT'ACT
Cuts at: 3401 3401
Size: 3884
SfcI C'TryA_G
Cuts at: 33 255 565 693 927 1612 2605 2796 33
Size: 222 310 128 234 685 993 191 1121
Fragments arranged by size:
1121 993 685 310 234 222 191 128
SfoI GGC'GCC
Cuts at: 198 1316 1564 198
Size: 1118 248 2518
Fragments arranged by size:
2518 1118 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
SmaI CCC'GGG
Cuts at: 157 872 906 157
Size: 715 34 3135
Fragments arranged by size:
3135 715 34
SmlI C'TyrA_G
Cuts at: 1039 1954 2446 2708 1039
Size: 915 492 262 2215
Fragments arranged by size:
2215 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3884
SphI G_CATG'C
Cuts at: 937 1437 937
Size: 500 3384
StyI C'CwwG_G
Cuts at: 107 494 755 854 1018 1372 107
Size: 387 261 99 164 354 2619
Fragments arranged by size:
2619 387 354 261 164 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1467 1600 2059 2268 1467
Size: 133 459 209 3083
Fragments arranged by size:
3083 459 209 133
TatI w'GTAC_w
Cuts at: 776 3399 776
Size: 2623 1261
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 562 594 3401 3433 562
Size: 32 2807 32 1013
Fragments arranged by size:
2807 1013 32 32
Tth111I GACn'n_nGTC
Cuts at: 1681 1681
Size: 3884
XbaI T'CTAG_A
Cuts at: 915 915
Size: 3884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 501 1379 501
Size: 878 3006
XmaI C'CCGG_G
Cuts at: 155 870 904 155
Size: 715 34 3135
Fragments arranged by size:
3135 715 34
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BbvCI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI