Restriction map for
pF1KB8778
(Circular) (Six-base) MAPSORT of: pF1KB8778.seq Check: 733 from: 1 to: 3887
pF1KB8778 3887 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 432 432
Size: 3887
AccI GT'mk_AC
Cuts at: 925 925
Size: 3887
Acc65I G'GTAC_C
Cuts at: 903 903
Size: 3887
AclI AA'CG_TT
Cuts at: 3727 3727
Size: 3887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1712 2144 2891 3159 1712
Size: 432 747 268 2440
Fragments arranged by size:
2440 747 432 268
AfeI AGC'GCT
Cuts at: 400 400
Size: 3887
AflIII A'CryG_T
Cuts at: 278 1239 2343 3320 278
Size: 961 1104 977 845
Fragments arranged by size:
1104 977 961 845
AgeI A'CCGG_T
Cuts at: 2275 2275
Size: 3887
AlwNI CAG_nnn'CTG
Cuts at: 297 447 661 1192 2759 297
Size: 150 214 531 1567 1425
Fragments arranged by size:
1567 1425 531 214 150
ApaI G_GGCC'C
Cuts at: 236 618 782 236
Size: 382 164 3341
Fragments arranged by size:
3341 382 164
ApaLI G'TGCA_C
Cuts at: 2657 2657
Size: 3887
ApoI r'AATT_y
Cuts at: 891 3240 3496 891
Size: 2349 256 1282
Fragments arranged by size:
2349 1282 256
AscI GG'CGCG_CC
Cuts at: 364 364
Size: 3887
AseI AT'TA_AT
Cuts at: 20 20
Size: 3887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
AvaI C'yCGr_G
Cuts at: 229 249 268 611 635 907 229
Size: 20 19 343 24 272 3209
Fragments arranged by size:
3209 343 272 24 20 19
BamHI G'GATC_C
Cuts at: 912 912
Size: 3887
BanI G'GyrC_C
Cuts at: 532 903 1317 1565 1600 532
Size: 371 414 248 35 2819
Fragments arranged by size:
2819 414 371 248 35
BanII G_rGCy'C
Cuts at: 236 355 618 782 815 901 1931 236
Size: 119 263 164 33 86 1030 2192
Fragments arranged by size:
2192 1030 263 164 119 86 33
BbeI G_GCGC'C
Cuts at: 536 1321 1569 536
Size: 785 248 2854
Fragments arranged by size:
2854 785 248
BbsI GAAGACnn'nnnn_
Cuts at: 721 721
Size: 3887
BbvCI CC'TCA_GC
Cuts at: 262 262
Size: 3887
BciVI GTATCCnnnnn_n'
Cuts at: 1785 2546 3887 1785
Size: 761 1341 1785
Fragments arranged by size:
1785 1341 761
BclI T'GATC_A
Cuts at: 1407 1407
Size: 3887
BglI GCCn_nnn'nGGC
Cuts at: 313 313
Size: 3887
BglII A'GATC_T
Cuts at: 1402 3439 1402
Size: 2037 1850
BlpI GC'TnA_GC
Cuts at: 999 999
Size: 3887
Bme1580I G_kGCm'C
Cuts at: 236 554 618 782 1512 1605 2661 236
Size: 318 64 164 730 93 1056 1462
Fragments arranged by size:
1462 1056 730 318 164 93 64
BmrI ACTGGGnnnn_n'
Cuts at: 1099 1180 1515 3659 1099
Size: 81 335 2144 1327
Fragments arranged by size:
2144 1327 335 81
BmtI G_CTAG'C
Cuts at: 209 209
Size: 3887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 3887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 481
Size: 3887
Bpu10I CC'TnA_GC
Cuts at: 262 262
Size: 3887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1942 2434 2732 1063
Size: 879 492 298 2218
Fragments arranged by size:
2218 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 814 3591 814
Size: 2777 1110
BsaAI yAC'GTr
Cuts at: 1240 1240
Size: 3887
BsaBI GATnn'nnATC
Cuts at: 6 1401 3438 6
Size: 1395 2037 455
Fragments arranged by size:
2037 1395 455
BsaHI Gr'CG_yC
Cuts at: 533 1318 1566 2268 3764 533
Size: 785 248 702 1496 656
Fragments arranged by size:
1496 785 702 656 248
BsaWI w'CCGG_w
Cuts at: 393 1076 1597 2275 2549 2696 3107 393
Size: 683 521 678 274 147 411 1173
Fragments arranged by size:
1173 683 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 462 492 631 657 661 687 462
Size: 30 139 26 4 26 3662
Fragments arranged by size:
3662 139 30 26 26 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 104 163 237 772 104
Size: 59 74 535 3219
Fragments arranged by size:
3219 535 74 59
BseYI C'CCAG_C
Cuts at: 447 654 661 1312 2070 2647 3143 447
Size: 207 7 651 758 577 496 1191
Fragments arranged by size:
1191 758 651 577 496 207 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 303
Size: 3887
BsiEI CG_ry'CG
Cuts at: 104 1429 1475 2683 104
Size: 1325 46 1208 1308
Fragments arranged by size:
1325 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 815 901 1679 1869 2661 815
Size: 86 778 190 792 2041
Fragments arranged by size:
2041 792 778 190 86
Bsp1286I G_dGCh'C
Cuts at: 236 355 554 618 782 815 901 1512 1605
Size: 119 199 64 164 33 86 611 93
Cuts at: 1605 1679 1869 1931 2661 236
Size: 74 190 62 730 1462
Fragments arranged by size:
1462 730 611 199 190 164 119 93
86 74 64 62 33
BspEI T'CCGG_A
Cuts at: 393 3107 393
Size: 2714 1173
BspHI T'CATG_A
Cuts at: 3063 3063
Size: 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 432 937 1151 1453 1834 432
Size: 505 214 302 381 2485
Fragments arranged by size:
2485 505 381 302 214
BsrBI CCG'CTC
Cuts at: 50 418 495 2179 2233 3717 3882 50
Size: 368 77 1684 54 1484 165 55
Fragments arranged by size:
1684 1484 368 165 77 55 54
BsrDI GCAATG_nn'
Cuts at: 1799 1799
Size: 3887
BsrFI r'CCGG_y
Cuts at: 222 1885 2066 2275 222
Size: 1663 181 209 1834
Fragments arranged by size:
1834 1663 209 181
BssHII G'CGCG_C
Cuts at: 364 364
Size: 3887
BssSI C'ACGA_G
Cuts at: 2158 2516 2158
Size: 358 3529
BstAPI GCAn_nnn'nTGC
Cuts at: 297 1264 297
Size: 967 2920
BstBI TT'CG_AA
Cuts at: 2248 2248
Size: 3887
BstYI r'GATC_y
Cuts at: 1 912 1379 1402 1737 1983 2995 3081 3093
Size: 911 467 23 335 246 1012 86 12
Cuts at: 3093 3439 1
Size: 346 449
Fragments arranged by size:
1012 911 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 384 528 767 384
Size: 144 239 3504
Fragments arranged by size:
3504 239 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1837 2018 2181 1837
Size: 181 163 3543
Fragments arranged by size:
3543 181 163
BtsI GCAGTG_nn'
Cuts at: 1130 1130
Size: 3887
DraI TTT'AAA
Cuts at: 886 886
Size: 3887
DrdI GACnn_nn'nnGTC
Cuts at: 1593 2451 1593
Size: 858 3029
EaeI y'GGCC_r
Cuts at: 1472 1646 2037 2064 3409 1472
Size: 174 391 27 1345 1950
Fragments arranged by size:
1950 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1472 1472
Size: 3887
EarI CTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 232 645 2095 2405 2551 3697 232
Size: 413 1450 310 146 1146 422
Fragments arranged by size:
1450 1146 422 413 310 146
EcoICRI GAG'CTC
Cuts at: 813 899 813
Size: 86 3801
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 1712 2144 2891 3159 481
Size: 1231 432 747 268 1209
Fragments arranged by size:
1231 1209 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 708 708
Size: 3887
EcoO109I rG'GnC_Cy
Cuts at: 473 665 1026 473
Size: 192 361 3334
Fragments arranged by size:
3334 361 192
EcoRI G'AATT_C
Cuts at: 891 891
Size: 3887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 759 1906 759
Size: 1147 2740
FspI TGC'GCA
Cuts at: 405 460 1668 405
Size: 55 1208 2624
Fragments arranged by size:
2624 1208 55
HaeII r_GCGC'y
Cuts at: 340 402 536 1321 1569 2591 3570 340
Size: 62 134 785 248 1022 979 657
Fragments arranged by size:
1022 979 785 657 248 134 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 462 494 631 657 663 689 1729 1759 1761
Size: 32 137 26 6 26 1040 30 2
Cuts at: 1761 1791 462
Size: 30 2558
Fragments arranged by size:
2558 1040 137 32 30 30 26 26
6 2
HincII GTy'rAC
Cuts at: 926 926
Size: 3887
KasI G'GCGC_C
Cuts at: 532 1317 1565 532
Size: 785 248 2854
Fragments arranged by size:
2854 785 248
KpnI G_GTAC'C
Cuts at: 907 907
Size: 3887
MluI A'CGCG_T
Cuts at: 278 3320 278
Size: 3042 845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2558 2742 2558
Size: 184 3703
MscI TGG'CCA
Cuts at: 1648 1648
Size: 3887
MslI CAynn'nnrTG
Cuts at: 1876 2003 1876
Size: 127 3760
MspA1I CmG'CkG
Cuts at: 287 322 386 661 998 1312 1672 2685 2930
Size: 35 64 275 337 314 360 1013 245
Cuts at: 2930 287
Size: 1244
Fragments arranged by size:
1244 1013 360 337 314 275 245 64
35
NaeI GCC'GGC
Cuts at: 2068 2068
Size: 3887
NarI GG'CG_CC
Cuts at: 533 1318 1566 533
Size: 785 248 2854
Fragments arranged by size:
2854 785 248
NcoI C'CATG_G
Cuts at: 767 767
Size: 3887
NgoMIV G'CCGG_C
Cuts at: 2066 2066
Size: 3887
NheI G'CTAG_C
Cuts at: 205 205
Size: 3887
NspI r_CATG'y
Cuts at: 940 1440 2347 940
Size: 500 907 2480
Fragments arranged by size:
2480 907 500
PciI A'CATG_T
Cuts at: 2343 2343
Size: 3887
PflMI CCAn_nnn'nTGG
Cuts at: 661 661
Size: 3887
PmeI GTTT'AAAC
Cuts at: 886 886
Size: 3887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 3855
PpuMI rG'GwC_Cy
Cuts at: 665 665
Size: 3887
PspOMI G'GGCC_C
Cuts at: 232 614 778 232
Size: 382 164 3341
Fragments arranged by size:
3341 382 164
PstI C_TGCA'G
Cuts at: 934 1619 934
Size: 685 3202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3887
PvuII CAG'CTG
Cuts at: 287 1312 1672 287
Size: 1025 360 2502
Fragments arranged by size:
2502 1025 360
RsrII CG'GwC_CG
Cuts at: 2082 2082
Size: 3887
SacI G_AGCT'C
Cuts at: 815 901 815
Size: 86 3801
SacII CC_GC'GG
Cuts at: 387 387
Size: 3887
SalI G'TCGA_C
Cuts at: 924 924
Size: 3887
SanDI GG'GwC_CC
Cuts at: 665 665
Size: 3887
SapI GCTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
SbfI CC_TGCA'GG
Cuts at: 934 934
Size: 3887
ScaI AGT'ACT
Cuts at: 3404 3404
Size: 3887
SfcI C'TryA_G
Cuts at: 33 930 1615 2608 2799 33
Size: 897 685 993 191 1121
Fragments arranged by size:
1121 993 897 685 191
SfoI GGC'GCC
Cuts at: 534 1319 1567 534
Size: 785 248 2854
Fragments arranged by size:
2854 785 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
SmaI CCC'GGG
Cuts at: 231 613 637 909 231
Size: 382 24 272 3209
Fragments arranged by size:
3209 382 272 24
SmlI C'TyrA_G
Cuts at: 1042 1957 2449 2711 1042
Size: 915 492 262 2218
Fragments arranged by size:
2218 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3887
SphI G_CATG'C
Cuts at: 940 1440 940
Size: 500 3387
StyI C'CwwG_G
Cuts at: 743 767 1021 1375 743
Size: 24 254 354 3255
Fragments arranged by size:
3255 354 254 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1470 1603 2062 2271 1470
Size: 133 459 209 3086
Fragments arranged by size:
3086 459 209 133
TatI w'GTAC_w
Cuts at: 3402 3402
Size: 3887
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 3855
Tth111I GACn'n_nGTC
Cuts at: 1684 1684
Size: 3887
XbaI T'CTAG_A
Cuts at: 918 918
Size: 3887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1382 1382
Size: 3887
XmaI C'CCGG_G
Cuts at: 229 611 635 907 229
Size: 382 24 272 3209
Fragments arranged by size:
3209 382 272 24
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AvrII BaeI
BcgI BfrBI BmgBI BsiWI BsmI BsmBI BsrGI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspXI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI