Restriction map for
pF1KB8779
(Circular) (Six-base) MAPSORT of: pF1KB8779.seq Check: 7133 from: 1 to: 3890
pF1KB8779 3890 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 119 928 119
Size: 809 3081
Acc65I G'GTAC_C
Cuts at: 906 906
Size: 3890
AclI AA'CG_TT
Cuts at: 3730 3730
Size: 3890
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 1715 2147 2894 3162 535
Size: 1180 432 747 268 1263
Fragments arranged by size:
1263 1180 747 432 268
AflIII A'CryG_T
Cuts at: 1242 2346 3323 1242
Size: 1104 977 1809
Fragments arranged by size:
1809 1104 977
AgeI A'CCGG_T
Cuts at: 2278 2278
Size: 3890
AleI CACnn'nnGTG
Cuts at: 754 754
Size: 3890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 515 515
Size: 3890
AlwNI CAG_nnn'CTG
Cuts at: 459 1195 2762 459
Size: 736 1567 1587
Fragments arranged by size:
1587 1567 736
ApaLI G'TGCA_C
Cuts at: 490 2660 490
Size: 2170 1720
ApoI r'AATT_y
Cuts at: 353 894 3243 3499 353
Size: 541 2349 256 744
Fragments arranged by size:
2349 744 541 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3890
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3890
AvaI C'yCGr_G
Cuts at: 910 910
Size: 3890
BamHI G'GATC_C
Cuts at: 915 915
Size: 3890
BanI G'GyrC_C
Cuts at: 906 1320 1568 1603 906
Size: 414 248 35 3193
Fragments arranged by size:
3193 414 248 35
BanII G_rGCy'C
Cuts at: 904 1934 904
Size: 1030 2860
BbeI G_GCGC'C
Cuts at: 1324 1572 1324
Size: 248 3642
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 406 440 406
Size: 34 3856
BciVI GTATCCnnnnn_n'
Cuts at: 1788 2549 3890 1788
Size: 761 1341 1788
Fragments arranged by size:
1788 1341 761
BclI T'GATC_A
Cuts at: 1410 1410
Size: 3890
BglI GCCn_nnn'nGGC
Cuts at: 840 840
Size: 3890
BglII A'GATC_T
Cuts at: 288 1405 3442 288
Size: 1117 2037 736
Fragments arranged by size:
2037 1117 736
BlpI GC'TnA_GC
Cuts at: 1002 1002
Size: 3890
Bme1580I G_kGCm'C
Cuts at: 494 1515 1608 2664 494
Size: 1021 93 1056 1720
Fragments arranged by size:
1720 1056 1021 93
BmrI ACTGGGnnnn_n'
Cuts at: 1102 1183 1518 3662 1102
Size: 81 335 2144 1330
Fragments arranged by size:
2144 1330 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 3890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 1066 1945 2437 2735 256
Size: 810 879 492 298 1411
Fragments arranged by size:
1411 879 810 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3594 3594
Size: 3890
BsaAI yAC'GTr
Cuts at: 1243 1243
Size: 3890
BsaBI GATnn'nnATC
Cuts at: 6 1404 3441 6
Size: 1398 2037 455
Fragments arranged by size:
2037 1398 455
BsaHI Gr'CG_yC
Cuts at: 589 1321 1569 2271 3767 589
Size: 732 248 702 1496 712
Fragments arranged by size:
1496 732 712 702 248
BsaWI w'CCGG_w
Cuts at: 1079 1600 2278 2552 2699 3110 1079
Size: 521 678 274 147 411 1859
Fragments arranged by size:
1859 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 594 145
Size: 449 3441
BseYI C'CCAG_C
Cuts at: 1315 2073 2650 3146 1315
Size: 758 577 496 2059
Fragments arranged by size:
2059 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1432 1478 2686 104
Size: 1328 46 1208 1308
Fragments arranged by size:
1328 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 494 904 1682 1872 2664 494
Size: 410 778 190 792 1720
Fragments arranged by size:
1720 792 778 410 190
Bsp1286I G_dGCh'C
Cuts at: 494 904 1515 1608 1682 1872 1934 2664 494
Size: 410 611 93 74 190 62 730 1720
Fragments arranged by size:
1720 730 611 410 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3110 3110
Size: 3890
BspHI T'CATG_A
Cuts at: 597 3066 597
Size: 2469 1421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 940 1154 1456 1837 940
Size: 214 302 381 2993
Fragments arranged by size:
2993 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2182 2236 3720 3885 50
Size: 2132 54 1484 165 55
Fragments arranged by size:
2132 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 267 1802 267
Size: 1535 2355
BsrFI r'CCGG_y
Cuts at: 1888 2069 2278 1888
Size: 181 209 3500
Fragments arranged by size:
3500 209 181
BssSI C'ACGA_G
Cuts at: 2161 2519 2161
Size: 358 3532
BstAPI GCAn_nnn'nTGC
Cuts at: 1267 1267
Size: 3890
BstBI TT'CG_AA
Cuts at: 2251 2251
Size: 3890
BstEII G'GTnAC_C
Cuts at: 729 729
Size: 3890
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3890
BstYI r'GATC_y
Cuts at: 1 288 915 1382 1405 1740 1986 2998 3084
Size: 287 627 467 23 335 246 1012 86
Cuts at: 3084 3096 3442 1
Size: 12 346 449
Fragments arranged by size:
1012 627 467 449 346 335 287 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1840 2021 2184 1840
Size: 181 163 3546
Fragments arranged by size:
3546 181 163
BtsI GCAGTG_nn'
Cuts at: 549 1133 549
Size: 584 3306
ClaI AT'CG_AT
Cuts at: 416 416
Size: 3890
DraI TTT'AAA
Cuts at: 245 889 245
Size: 644 3246
DraIII CAC_nnn'GTG
Cuts at: 615 615
Size: 3890
DrdI GACnn_nn'nnGTC
Cuts at: 1596 2454 1596
Size: 858 3032
EaeI y'GGCC_r
Cuts at: 1475 1649 2040 2067 3412 1475
Size: 174 391 27 1345 1953
Fragments arranged by size:
1953 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1475 1475
Size: 3890
EarI CTCTTCn'nnn_
Cuts at: 1913 2123 1913
Size: 210 3680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2098 2408 2554 3700 2098
Size: 310 146 1146 2288
Fragments arranged by size:
2288 1146 310 146
EcoICRI GAG'CTC
Cuts at: 902 902
Size: 3890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 1715 2147 2894 3162 535
Size: 1180 432 747 268 1263
Fragments arranged by size:
1263 1180 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 363 1029 363
Size: 666 3224
EcoRI G'AATT_C
Cuts at: 894 894
Size: 3890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1909 1909
Size: 3890
FspI TGC'GCA
Cuts at: 1671 1671
Size: 3890
HaeII r_GCGC'y
Cuts at: 1324 1572 2594 3573 1324
Size: 248 1022 979 1641
Fragments arranged by size:
1641 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 647 679 774 806 1732 1762 1764 1794 647
Size: 32 95 32 926 30 2 30 2743
Fragments arranged by size:
2743 926 95 32 32 30 30 2
HincII GTy'rAC
Cuts at: 131 929 131
Size: 798 3092
HpaI GTT'AAC
Cuts at: 131 131
Size: 3890
KasI G'GCGC_C
Cuts at: 1320 1568 1320
Size: 248 3642
KpnI G_GTAC'C
Cuts at: 910 910
Size: 3890
MluI A'CGCG_T
Cuts at: 3323 3323
Size: 3890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 494 617 2561 2745 494
Size: 123 1944 184 1639
Fragments arranged by size:
1944 1639 184 123
MscI TGG'CCA
Cuts at: 1651 1651
Size: 3890
MslI CAynn'nnrTG
Cuts at: 497 754 1879 2006 497
Size: 257 1125 127 2381
Fragments arranged by size:
2381 1125 257 127
MspA1I CmG'CkG
Cuts at: 1001 1315 1675 2688 2933 1001
Size: 314 360 1013 245 1958
Fragments arranged by size:
1958 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2071 2071
Size: 3890
NarI GG'CG_CC
Cuts at: 1321 1569 1321
Size: 248 3642
NdeI CA'TA_TG
Cuts at: 765 765
Size: 3890
NgoMIV G'CCGG_C
Cuts at: 2069 2069
Size: 3890
NspI r_CATG'y
Cuts at: 427 649 943 1443 2350 427
Size: 222 294 500 907 1967
Fragments arranged by size:
1967 907 500 294 222
PciI A'CATG_T
Cuts at: 2346 2346
Size: 3890
PmeI GTTT'AAAC
Cuts at: 889 889
Size: 3890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 3858
PpuMI rG'GwC_Cy
Cuts at: 363 363
Size: 3890
PstI C_TGCA'G
Cuts at: 937 1622 937
Size: 685 3205
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3890
PvuII CAG'CTG
Cuts at: 1315 1675 1315
Size: 360 3530
RsrII CG'GwC_CG
Cuts at: 2085 2085
Size: 3890
SacI G_AGCT'C
Cuts at: 904 904
Size: 3890
SalI G'TCGA_C
Cuts at: 927 927
Size: 3890
SapI GCTCTTCn'nnn_
Cuts at: 1913 2123 1913
Size: 210 3680
SbfI CC_TGCA'GG
Cuts at: 937 937
Size: 3890
ScaI AGT'ACT
Cuts at: 3407 3407
Size: 3890
SfcI C'TryA_G
Cuts at: 33 255 565 933 1618 2611 2802 33
Size: 222 310 368 685 993 191 1121
Fragments arranged by size:
1121 993 685 368 310 222 191
SfoI GGC'GCC
Cuts at: 1322 1570 1322
Size: 248 3642
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3890
SmaI CCC'GGG
Cuts at: 912 912
Size: 3890
SmlI C'TyrA_G
Cuts at: 271 1045 1960 2452 2714 271
Size: 774 915 492 262 1447
Fragments arranged by size:
1447 915 774 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3890
SphI G_CATG'C
Cuts at: 427 943 1443 427
Size: 516 500 2874
Fragments arranged by size:
2874 516 500
StyI C'CwwG_G
Cuts at: 212 1024 1378 212
Size: 812 354 2724
Fragments arranged by size:
2724 812 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1473 1606 2065 2274 1473
Size: 133 459 209 3089
Fragments arranged by size:
3089 459 209 133
TatI w'GTAC_w
Cuts at: 559 3405 559
Size: 2846 1044
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 327 359 471 503 3407 3439 327
Size: 32 112 32 2904 32 778
Fragments arranged by size:
2904 778 112 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1687 1687
Size: 3890
XbaI T'CTAG_A
Cuts at: 921 921
Size: 3890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1385 1385
Size: 3890
XmaI C'CCGG_G
Cuts at: 910 910
Size: 3890
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I Bsu36I BtgI CspCI EcoNI EcoRV FseI FspAI
HindIII MfeI NcoI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI