Restriction map for
pF1KB8780
(Circular) (Six-base) MAPSORT of: pF1KB8780.seq Check: 1832 from: 1 to: 3890
pF1KB8780 3890 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 928 928
Size: 3890
Acc65I G'GTAC_C
Cuts at: 906 906
Size: 3890
AclI AA'CG_TT
Cuts at: 3730 3730
Size: 3890
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 235 1715 2147 2894 3162 190
Size: 45 1480 432 747 268 918
Fragments arranged by size:
1480 918 747 432 268 45
AflIII A'CryG_T
Cuts at: 1242 2346 3323 1242
Size: 1104 977 1809
Fragments arranged by size:
1809 1104 977
AgeI A'CCGG_T
Cuts at: 2278 2278
Size: 3890
AlwNI CAG_nnn'CTG
Cuts at: 168 459 1195 2762 168
Size: 291 736 1567 1296
Fragments arranged by size:
1567 1296 736 291
ApaI G_GGCC'C
Cuts at: 316 865 316
Size: 549 3341
ApaLI G'TGCA_C
Cuts at: 2660 2660
Size: 3890
ApoI r'AATT_y
Cuts at: 894 3243 3499 894
Size: 2349 256 1285
Fragments arranged by size:
2349 1285 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3890
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3890
AvaI C'yCGr_G
Cuts at: 441 910 441
Size: 469 3421
BamHI G'GATC_C
Cuts at: 915 915
Size: 3890
BanI G'GyrC_C
Cuts at: 906 1320 1568 1603 906
Size: 414 248 35 3193
Fragments arranged by size:
3193 414 248 35
BanII G_rGCy'C
Cuts at: 316 865 904 1934 316
Size: 549 39 1030 2272
Fragments arranged by size:
2272 1030 549 39
BbeI G_GCGC'C
Cuts at: 1324 1572 1324
Size: 248 3642
BbsI GAAGACnn'nnnn_
Cuts at: 873 873
Size: 3890
BciVI GTATCCnnnnn_n'
Cuts at: 1788 2549 3890 1788
Size: 761 1341 1788
Fragments arranged by size:
1788 1341 761
BclI T'GATC_A
Cuts at: 1410 1410
Size: 3890
BglI GCCn_nnn'nGGC
Cuts at: 691 691
Size: 3890
BglII A'GATC_T
Cuts at: 1405 3442 1405
Size: 2037 1853
BlpI GC'TnA_GC
Cuts at: 514 1002 514
Size: 488 3402
Bme1580I G_kGCm'C
Cuts at: 316 865 1515 1608 2664 316
Size: 549 650 93 1056 1542
Fragments arranged by size:
1542 1056 650 549 93
BmrI ACTGGGnnnn_n'
Cuts at: 555 729 1102 1183 1518 3662 555
Size: 174 373 81 335 2144 783
Fragments arranged by size:
2144 783 373 335 174 81
BmtI G_CTAG'C
Cuts at: 340 340
Size: 3890
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 3890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 217 292 397 499 651 775 101
Size: 116 75 105 102 152 124 3216
Fragments arranged by size:
3216 152 124 116 105 102 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 1945 2437 2735 1066
Size: 879 492 298 2221
Fragments arranged by size:
2221 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 491 632 3594 491
Size: 141 2962 787
Fragments arranged by size:
2962 787 141
BsaAI yAC'GTr
Cuts at: 1243 1243
Size: 3890
BsaBI GATnn'nnATC
Cuts at: 6 1404 3441 6
Size: 1398 2037 455
Fragments arranged by size:
2037 1398 455
BsaHI Gr'CG_yC
Cuts at: 1321 1569 2271 3767 1321
Size: 248 702 1496 1444
Fragments arranged by size:
1496 1444 702 248
BsaWI w'CCGG_w
Cuts at: 1079 1600 2278 2552 2699 3110 1079
Size: 521 678 274 147 411 1859
Fragments arranged by size:
1859 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 772 772
Size: 3890
BseYI C'CCAG_C
Cuts at: 161 463 661 695 733 797 1315 2073 2650
Size: 302 198 34 38 64 518 758 577
Cuts at: 2650 3146 161
Size: 496 905
Fragments arranged by size:
905 758 577 518 496 302 198 64
38 34
BsiEI CG_ry'CG
Cuts at: 104 1432 1478 2686 104
Size: 1328 46 1208 1308
Fragments arranged by size:
1328 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 347 904 1682 1872 2664 347
Size: 557 778 190 792 1573
Fragments arranged by size:
1573 792 778 557 190
Bsp1286I G_dGCh'C
Cuts at: 316 347 865 904 1515 1608 1682 1872 1934
Size: 31 518 39 611 93 74 190 62
Cuts at: 1934 2664 316
Size: 730 1542
Fragments arranged by size:
1542 730 611 518 190 93 74 62
39 31
BspEI T'CCGG_A
Cuts at: 3110 3110
Size: 3890
BspHI T'CATG_A
Cuts at: 3066 3066
Size: 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 494 834 940 1154 1456 1837 494
Size: 340 106 214 302 381 2547
Fragments arranged by size:
2547 381 340 302 214 106
BsrBI CCG'CTC
Cuts at: 50 525 2182 2236 3720 3885 50
Size: 475 1657 54 1484 165 55
Fragments arranged by size:
1657 1484 475 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1802 1802
Size: 3890
BsrFI r'CCGG_y
Cuts at: 767 1888 2069 2278 767
Size: 1121 181 209 2379
Fragments arranged by size:
2379 1121 209 181
BssSI C'ACGA_G
Cuts at: 2161 2519 2161
Size: 358 3532
BstAPI GCAn_nnn'nTGC
Cuts at: 1267 1267
Size: 3890
BstBI TT'CG_AA
Cuts at: 2251 2251
Size: 3890
BstXI CCAn_nnnn'nTGG
Cuts at: 719 719
Size: 3890
BstYI r'GATC_y
Cuts at: 1 915 1382 1405 1740 1986 2998 3084 3096
Size: 914 467 23 335 246 1012 86 12
Cuts at: 3096 3442 1
Size: 346 449
Fragments arranged by size:
1012 914 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3890
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1840 2021 2184 1840
Size: 181 163 3546
Fragments arranged by size:
3546 181 163
BtsI GCAGTG_nn'
Cuts at: 460 784 1133 460
Size: 324 349 3217
Fragments arranged by size:
3217 349 324
DraI TTT'AAA
Cuts at: 889 889
Size: 3890
DrdI GACnn_nn'nnGTC
Cuts at: 1596 2454 1596
Size: 858 3032
EaeI y'GGCC_r
Cuts at: 386 434 1475 1649 2040 2067 3412 386
Size: 48 1041 174 391 27 1345 864
Fragments arranged by size:
1345 1041 864 391 174 48 27
EagI C'GGCC_G
Cuts at: 1475 1475
Size: 3890
EarI CTCTTCn'nnn_
Cuts at: 876 1913 2123 876
Size: 1037 210 2643
Fragments arranged by size:
2643 1037 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 158 853 2098 2408 2554 3700 158
Size: 695 1245 310 146 1146 348
Fragments arranged by size:
1245 1146 695 348 310 146
EcoICRI GAG'CTC
Cuts at: 902 902
Size: 3890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 190 217 235 292 397 499 651 775
Size: 89 27 18 57 105 102 152 124
Cuts at: 775 1715 2147 2894 3162 101
Size: 940 432 747 268 829
Fragments arranged by size:
940 829 747 432 268 152 124 105
102 89 57 27 18
EcoNI CCTnn'n_nnAGG
Cuts at: 757 832 757
Size: 75 3815
EcoO109I rG'GnC_Cy
Cuts at: 676 801 862 1029 676
Size: 125 61 167 3537
Fragments arranged by size:
3537 167 125 61
EcoRI G'AATT_C
Cuts at: 894 894
Size: 3890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1909 1909
Size: 3890
FspI TGC'GCA
Cuts at: 1671 1671
Size: 3890
HaeII r_GCGC'y
Cuts at: 1324 1572 2594 3573 1324
Size: 248 1022 979 1641
Fragments arranged by size:
1641 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 137 169 1732 1762 1764 1794 137
Size: 32 1563 30 2 30 2233
Fragments arranged by size:
2233 1563 32 30 30 2
HincII GTy'rAC
Cuts at: 929 929
Size: 3890
KasI G'GCGC_C
Cuts at: 1320 1568 1320
Size: 248 3642
KpnI G_GTAC'C
Cuts at: 910 910
Size: 3890
MluI A'CGCG_T
Cuts at: 3323 3323
Size: 3890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 380 2561 2745 380
Size: 2181 184 1525
Fragments arranged by size:
2181 1525 184
MscI TGG'CCA
Cuts at: 388 436 1651 388
Size: 48 1215 2627
Fragments arranged by size:
2627 1215 48
MslI CAynn'nnrTG
Cuts at: 1879 2006 1879
Size: 127 3763
MspA1I CmG'CkG
Cuts at: 121 165 318 450 630 1001 1315 1675 2688
Size: 44 153 132 180 371 314 360 1013
Cuts at: 2688 2933 121
Size: 245 1078
Fragments arranged by size:
1078 1013 371 360 314 245 180 153
132 44
NaeI GCC'GGC
Cuts at: 769 2071 769
Size: 1302 2588
NarI GG'CG_CC
Cuts at: 1321 1569 1321
Size: 248 3642
NcoI C'CATG_G
Cuts at: 107 107
Size: 3890
NgoMIV G'CCGG_C
Cuts at: 767 2069 767
Size: 1302 2588
NheI G'CTAG_C
Cuts at: 336 336
Size: 3890
NspI r_CATG'y
Cuts at: 943 1443 2350 943
Size: 500 907 2483
Fragments arranged by size:
2483 907 500
PasI CC'CwG_GG
Cuts at: 701 701
Size: 3890
PciI A'CATG_T
Cuts at: 2346 2346
Size: 3890
PfoI T'CCnGG_A
Cuts at: 557 752 776 557
Size: 195 24 3671
Fragments arranged by size:
3671 195 24
PmeI GTTT'AAAC
Cuts at: 889 889
Size: 3890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 3858
PpuMI rG'GwC_Cy
Cuts at: 801 801
Size: 3890
PspOMI G'GGCC_C
Cuts at: 312 861 312
Size: 549 3341
PstI C_TGCA'G
Cuts at: 152 455 555 791 937 1622 152
Size: 303 100 236 146 685 2420
Fragments arranged by size:
2420 685 303 236 146 100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3890
PvuII CAG'CTG
Cuts at: 165 450 630 1315 1675 165
Size: 285 180 685 360 2380
Fragments arranged by size:
2380 685 360 285 180
RsrII CG'GwC_CG
Cuts at: 2085 2085
Size: 3890
SacI G_AGCT'C
Cuts at: 904 904
Size: 3890
SalI G'TCGA_C
Cuts at: 927 927
Size: 3890
SapI GCTCTTCn'nnn_
Cuts at: 1913 2123 1913
Size: 210 3680
SbfI CC_TGCA'GG
Cuts at: 937 937
Size: 3890
ScaI AGT'ACT
Cuts at: 3407 3407
Size: 3890
SfcI C'TryA_G
Cuts at: 33 148 451 551 787 933 1618 2611 2802
Size: 115 303 100 236 146 685 993 191
Cuts at: 2802 33
Size: 1121
Fragments arranged by size:
1121 993 685 303 236 191 146 115
100
SfoI GGC'GCC
Cuts at: 1322 1570 1322
Size: 248 3642
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3890
SmaI CCC'GGG
Cuts at: 443 912 443
Size: 469 3421
SmlI C'TyrA_G
Cuts at: 1045 1960 2452 2714 1045
Size: 915 492 262 2221
Fragments arranged by size:
2221 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3890
SphI G_CATG'C
Cuts at: 943 1443 943
Size: 500 3390
StuI AGG'CCT
Cuts at: 763 763
Size: 3890
StyI C'CwwG_G
Cuts at: 107 1024 1378 107
Size: 917 354 2619
Fragments arranged by size:
2619 917 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 319 693 1473 1606 2065 2274 319
Size: 374 780 133 459 209 1935
Fragments arranged by size:
1935 780 459 374 209 133
TatI w'GTAC_w
Cuts at: 3405 3405
Size: 3890
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3407 3439 3407
Size: 32 3858
Tth111I GACn'n_nGTC
Cuts at: 1687 1687
Size: 3890
XbaI T'CTAG_A
Cuts at: 921 921
Size: 3890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 825 1385 825
Size: 560 3330
XmaI C'CCGG_G
Cuts at: 441 910 441
Size: 469 3421
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PflMI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI