Restriction map for
pF1KB8781
(Circular) (Six-base) MAPSORT of: pF1KB8781.seq Check: 4705 from: 1 to: 3899
pF1KB8781 3899 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 937 937
Size: 3899
Acc65I G'GTAC_C
Cuts at: 915 915
Size: 3899
AclI AA'CG_TT
Cuts at: 3739 3739
Size: 3899
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 492 1724 2156 2903 3171 492
Size: 1232 432 747 268 1220
Fragments arranged by size:
1232 1220 747 432 268
AflIII A'CryG_T
Cuts at: 1251 2355 3332 1251
Size: 1104 977 1818
Fragments arranged by size:
1818 1104 977
AgeI A'CCGG_T
Cuts at: 2287 2287
Size: 3899
AleI CACnn'nnGTG
Cuts at: 779 779
Size: 3899
AlwNI CAG_nnn'CTG
Cuts at: 369 1204 2771 369
Size: 835 1567 1497
Fragments arranged by size:
1567 1497 835
ApaLI G'TGCA_C
Cuts at: 2669 2669
Size: 3899
ApoI r'AATT_y
Cuts at: 290 374 458 626 794 903 3252 3508 290
Size: 84 84 168 168 109 2349 256 681
Fragments arranged by size:
2349 681 256 168 168 109 84 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 3899
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3899
AvaI C'yCGr_G
Cuts at: 919 919
Size: 3899
BamHI G'GATC_C
Cuts at: 924 924
Size: 3899
BanI G'GyrC_C
Cuts at: 915 1329 1577 1612 915
Size: 414 248 35 3202
Fragments arranged by size:
3202 414 248 35
BanII G_rGCy'C
Cuts at: 436 913 1943 436
Size: 477 1030 2392
Fragments arranged by size:
2392 1030 477
BbeI G_GCGC'C
Cuts at: 1333 1581 1333
Size: 248 3651
BciVI GTATCCnnnnn_n'
Cuts at: 1797 2558 3899 1797
Size: 761 1341 1797
Fragments arranged by size:
1797 1341 761
BclI T'GATC_A
Cuts at: 1419 1419
Size: 3899
BglII A'GATC_T
Cuts at: 1414 3451 1414
Size: 2037 1862
BlpI GC'TnA_GC
Cuts at: 1011 1011
Size: 3899
Bme1580I G_kGCm'C
Cuts at: 1524 1617 2673 1524
Size: 93 1056 2750
Fragments arranged by size:
2750 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1111 1192 1527 3671 1111
Size: 81 335 2144 1339
Fragments arranged by size:
2144 1339 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 203 2266 203
Size: 2063 1836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 488 572 740 824 908 404
Size: 84 84 168 84 84 3395
Fragments arranged by size:
3395 168 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1954 2446 2744 1075
Size: 879 492 298 2230
Fragments arranged by size:
2230 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3603 3603
Size: 3899
BsaAI yAC'GTr
Cuts at: 1252 1252
Size: 3899
BsaBI GATnn'nnATC
Cuts at: 6 1413 3450 6
Size: 1407 2037 455
Fragments arranged by size:
2037 1407 455
BsaHI Gr'CG_yC
Cuts at: 1330 1578 2280 3776 1330
Size: 248 702 1496 1453
Fragments arranged by size:
1496 1453 702 248
BsaWI w'CCGG_w
Cuts at: 298 1088 1609 2287 2561 2708 3119 298
Size: 790 521 678 274 147 411 1078
Fragments arranged by size:
1078 790 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 230 230
Size: 3899
BseYI C'CCAG_C
Cuts at: 194 1324 2082 2659 3155 194
Size: 1130 758 577 496 938
Fragments arranged by size:
1130 938 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 255 151
Size: 104 3795
BsiEI CG_ry'CG
Cuts at: 104 1441 1487 2695 104
Size: 1337 46 1208 1308
Fragments arranged by size:
1337 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 913 1691 1881 2673 913
Size: 778 190 792 2139
Fragments arranged by size:
2139 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 436 913 1524 1617 1691 1881 1943 2673 436
Size: 477 611 93 74 190 62 730 1662
Fragments arranged by size:
1662 730 611 477 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3119 3119
Size: 3899
BspHI T'CATG_A
Cuts at: 3075 3075
Size: 3899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 949 1163 1465 1846 949
Size: 214 302 381 3002
Fragments arranged by size:
3002 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2191 2245 3729 3894 50
Size: 2141 54 1484 165 55
Fragments arranged by size:
2141 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 411 1811 411
Size: 1400 2499
BsrFI r'CCGG_y
Cuts at: 1897 2078 2287 1897
Size: 181 209 3509
Fragments arranged by size:
3509 209 181
BsrGI T'GTAC_A
Cuts at: 487 487
Size: 3899
BssSI C'ACGA_G
Cuts at: 2170 2528 2170
Size: 358 3541
BstAPI GCAn_nnn'nTGC
Cuts at: 1276 1276
Size: 3899
BstBI TT'CG_AA
Cuts at: 2260 2260
Size: 3899
BstYI r'GATC_y
Cuts at: 1 924 1391 1414 1749 1995 3007 3093 3105
Size: 923 467 23 335 246 1012 86 12
Cuts at: 3105 3451 1
Size: 346 449
Fragments arranged by size:
1012 923 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1849 2030 2193 1849
Size: 181 163 3555
Fragments arranged by size:
3555 181 163
BtsI GCAGTG_nn'
Cuts at: 243 1142 243
Size: 899 3000
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 393 428 393
Size: 35 3864
DraI TTT'AAA
Cuts at: 898 898
Size: 3899
DrdI GACnn_nn'nnGTC
Cuts at: 1605 2463 1605
Size: 858 3041
EaeI y'GGCC_r
Cuts at: 1484 1658 2049 2076 3421 1484
Size: 174 391 27 1345 1962
Fragments arranged by size:
1962 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1484 1484
Size: 3899
EarI CTCTTCn'nnn_
Cuts at: 693 1922 2132 693
Size: 1229 210 2460
Fragments arranged by size:
2460 1229 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2107 2417 2563 3709 2107
Size: 310 146 1146 2297
Fragments arranged by size:
2297 1146 310 146
EcoICRI GAG'CTC
Cuts at: 911 911
Size: 3899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 488 492 572 740 824 908 1724 2156
Size: 84 4 80 168 84 84 816 432
Cuts at: 2156 2903 3171 404
Size: 747 268 1132
Fragments arranged by size:
1132 816 747 432 268 168 84 84
84 80 4
EcoO109I rG'GnC_Cy
Cuts at: 1038 1038
Size: 3899
EcoRI G'AATT_C
Cuts at: 290 374 458 626 794 903 290
Size: 84 84 168 168 109 3286
Fragments arranged by size:
3286 168 168 109 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 373 793 1918 373
Size: 420 1125 2354
Fragments arranged by size:
2354 1125 420
FspI TGC'GCA
Cuts at: 1680 1680
Size: 3899
HaeII r_GCGC'y
Cuts at: 1333 1581 2603 3582 1333
Size: 248 1022 979 1650
Fragments arranged by size:
1650 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 591 623 1741 1771 1773 1803 591
Size: 32 1118 30 2 30 2687
Fragments arranged by size:
2687 1118 32 30 30 2
HincII GTy'rAC
Cuts at: 126 938 126
Size: 812 3087
HpaI GTT'AAC
Cuts at: 126 126
Size: 3899
KasI G'GCGC_C
Cuts at: 1329 1577 1329
Size: 248 3651
KpnI G_GTAC'C
Cuts at: 919 919
Size: 3899
MluI A'CGCG_T
Cuts at: 3332 3332
Size: 3899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 807 2570 2754 807
Size: 1763 184 1952
Fragments arranged by size:
1952 1763 184
MscI TGG'CCA
Cuts at: 1660 1660
Size: 3899
MslI CAynn'nnrTG
Cuts at: 483 779 1888 2015 483
Size: 296 1109 127 2367
Fragments arranged by size:
2367 1109 296 127
MspA1I CmG'CkG
Cuts at: 1010 1324 1684 2697 2942 1010
Size: 314 360 1013 245 1967
Fragments arranged by size:
1967 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2080 2080
Size: 3899
NarI GG'CG_CC
Cuts at: 1330 1578 1330
Size: 248 3651
NdeI CA'TA_TG
Cuts at: 520 520
Size: 3899
NgoMIV G'CCGG_C
Cuts at: 2078 2078
Size: 3899
NspI r_CATG'y
Cuts at: 608 656 952 1452 2359 608
Size: 48 296 500 907 2148
Fragments arranged by size:
2148 907 500 296 48
PciI A'CATG_T
Cuts at: 2355 2355
Size: 3899
PmeI GTTT'AAAC
Cuts at: 898 898
Size: 3899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3038 3070 3038
Size: 32 3867
PsiI TTA'TAA
Cuts at: 314 734 314
Size: 420 3479
PstI C_TGCA'G
Cuts at: 946 1631 946
Size: 685 3214
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3899
PvuII CAG'CTG
Cuts at: 1324 1684 1324
Size: 360 3539
RsrII CG'GwC_CG
Cuts at: 2094 2094
Size: 3899
SacI G_AGCT'C
Cuts at: 913 913
Size: 3899
SalI G'TCGA_C
Cuts at: 936 936
Size: 3899
SapI GCTCTTCn'nnn_
Cuts at: 1922 2132 1922
Size: 210 3689
SbfI CC_TGCA'GG
Cuts at: 946 946
Size: 3899
ScaI AGT'ACT
Cuts at: 3416 3416
Size: 3899
SfcI C'TryA_G
Cuts at: 33 942 1627 2620 2811 33
Size: 909 685 993 191 1121
Fragments arranged by size:
1121 993 909 685 191
SfoI GGC'GCC
Cuts at: 1331 1579 1331
Size: 248 3651
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3899
SmaI CCC'GGG
Cuts at: 921 921
Size: 3899
SmlI C'TyrA_G
Cuts at: 1054 1969 2461 2723 1054
Size: 915 492 262 2230
Fragments arranged by size:
2230 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3899
SphI G_CATG'C
Cuts at: 952 1452 952
Size: 500 3399
StyI C'CwwG_G
Cuts at: 1033 1387 1033
Size: 354 3545
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1482 1615 2074 2283 1482
Size: 133 459 209 3098
Fragments arranged by size:
3098 459 209 133
TatI w'GTAC_w
Cuts at: 487 3414 487
Size: 2927 972
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 3867
Tth111I GACn'n_nGTC
Cuts at: 1696 1696
Size: 3899
XbaI T'CTAG_A
Cuts at: 930 930
Size: 3899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1394 1394
Size: 3899
XmaI C'CCGG_G
Cuts at: 919 919
Size: 3899
XmnI GAAnn'nnTTC
Cuts at: 546 630 546
Size: 84 3815
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII MfeI NcoI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI