Restriction map for
pF1KB8782
(Circular) (Six-base) MAPSORT of: pF1KB8782.seq Check: 7103 from: 1 to: 3932
pF1KB8782 3932 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 970 970
Size: 3932
Acc65I G'GTAC_C
Cuts at: 214 948 214
Size: 734 3198
AclI AA'CG_TT
Cuts at: 3772 3772
Size: 3932
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1757 2189 2936 3204 1757
Size: 432 747 268 2485
Fragments arranged by size:
2485 747 432 268
AfeI AGC'GCT
Cuts at: 823 823
Size: 3932
AflIII A'CryG_T
Cuts at: 638 1284 2388 3365 638
Size: 646 1104 977 1205
Fragments arranged by size:
1205 1104 977 646
AgeI A'CCGG_T
Cuts at: 2320 2320
Size: 3932
AlwNI CAG_nnn'CTG
Cuts at: 1237 2804 1237
Size: 1567 2365
ApaLI G'TGCA_C
Cuts at: 325 502 2702 325
Size: 177 2200 1555
Fragments arranged by size:
2200 1555 177
ApoI r'AATT_y
Cuts at: 936 3285 3541 936
Size: 2349 256 1327
Fragments arranged by size:
2349 1327 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3932
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
AvaI C'yCGr_G
Cuts at: 646 808 952 646
Size: 162 144 3626
Fragments arranged by size:
3626 162 144
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 198 231 198
Size: 33 3899
BamHI G'GATC_C
Cuts at: 957 957
Size: 3932
BanI G'GyrC_C
Cuts at: 214 469 773 948 1362 1610 1645 214
Size: 255 304 175 414 248 35 2501
Fragments arranged by size:
2501 414 304 255 248 175 35
BanII G_rGCy'C
Cuts at: 541 946 1976 541
Size: 405 1030 2497
Fragments arranged by size:
2497 1030 405
BbeI G_GCGC'C
Cuts at: 473 777 1366 1614 473
Size: 304 589 248 2791
Fragments arranged by size:
2791 589 304 248
BbsI GAAGACnn'nnnn_
Cuts at: 324 324
Size: 3932
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 436 470 687 721 723 757 436
Size: 34 217 34 2 34 3611
Fragments arranged by size:
3611 217 34 34 34 2
BciVI GTATCCnnnnn_n'
Cuts at: 1830 2591 3932 1830
Size: 761 1341 1830
Fragments arranged by size:
1830 1341 761
BclI T'GATC_A
Cuts at: 1452 1452
Size: 3932
BglII A'GATC_T
Cuts at: 1447 3484 1447
Size: 2037 1895
BlpI GC'TnA_GC
Cuts at: 535 627 1044 535
Size: 92 417 3423
Fragments arranged by size:
3423 417 92
Bme1580I G_kGCm'C
Cuts at: 329 506 1557 1650 2706 329
Size: 177 1051 93 1056 1555
Fragments arranged by size:
1555 1056 1051 177 93
BmgBI CAC'GTC
Cuts at: 330 330
Size: 3932
BmrI ACTGGGnnnn_n'
Cuts at: 1144 1225 1560 3704 1144
Size: 81 335 2144 1372
Fragments arranged by size:
2144 1372 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 3932
Bpu10I CC'TnA_GC
Cuts at: 607 607
Size: 3932
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 1108 1987 2479 2777 499
Size: 609 879 492 298 1654
Fragments arranged by size:
1654 879 609 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3636 3636
Size: 3932
BsaAI yAC'GTr
Cuts at: 1285 1285
Size: 3932
BsaBI GATnn'nnATC
Cuts at: 6 1446 3483 6
Size: 1440 2037 455
Fragments arranged by size:
2037 1440 455
BsaHI Gr'CG_yC
Cuts at: 470 704 774 1363 1611 2313 3809 470
Size: 234 70 589 248 702 1496 593
Fragments arranged by size:
1496 702 593 589 248 234 70
BsaWI w'CCGG_w
Cuts at: 1121 1642 2320 2594 2741 3152 1121
Size: 521 678 274 147 411 1901
Fragments arranged by size:
1901 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 147 1357 2115 2692 3188 147
Size: 1210 758 577 496 891
Fragments arranged by size:
1210 891 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 469 430
Size: 39 3893
BsiEI CG_ry'CG
Cuts at: 104 386 582 1474 1520 2728 104
Size: 282 196 892 46 1208 1308
Fragments arranged by size:
1308 1208 892 282 196 46
BsiHKAI G_wGCw'C
Cuts at: 329 506 613 656 713 794 946 1724 1914
Size: 177 107 43 57 81 152 778 190
Cuts at: 1914 2706 329
Size: 792 1555
Fragments arranged by size:
1555 792 778 190 177 152 107 81
57 43
Bsp1286I G_dGCh'C
Cuts at: 329 506 541 613 656 713 794 946 1557
Size: 177 35 72 43 57 81 152 611
Cuts at: 1557 1650 1724 1914 1976 2706 329
Size: 93 74 190 62 730 1555
Fragments arranged by size:
1555 730 611 190 177 152 93 81
74 72 62 57 43 35
BspEI T'CCGG_A
Cuts at: 3152 3152
Size: 3932
BspHI T'CATG_A
Cuts at: 3108 3108
Size: 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 404 491 982 1196 1498 1879 404
Size: 87 491 214 302 381 2457
Fragments arranged by size:
2457 491 381 302 214 87
BsrBI CCG'CTC
Cuts at: 50 2224 2278 3762 3927 50
Size: 2174 54 1484 165 55
Fragments arranged by size:
2174 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1844 1844
Size: 3932
BsrFI r'CCGG_y
Cuts at: 239 466 760 1930 2111 2320 239
Size: 227 294 1170 181 209 1851
Fragments arranged by size:
1851 1170 294 227 209 181
BsrGI T'GTAC_A
Cuts at: 365 365
Size: 3932
BssHII G'CGCG_C
Cuts at: 442 892 909 442
Size: 450 17 3465
Fragments arranged by size:
3465 450 17
BssSI C'ACGA_G
Cuts at: 2203 2561 2203
Size: 358 3574
BstAPI GCAn_nnn'nTGC
Cuts at: 324 408 1309 324
Size: 84 901 2947
Fragments arranged by size:
2947 901 84
BstBI TT'CG_AA
Cuts at: 2293 2293
Size: 3932
BstEII G'GTnAC_C
Cuts at: 723 723
Size: 3932
BstXI CCAn_nnnn'nTGG
Cuts at: 231 592 231
Size: 361 3571
BstYI r'GATC_y
Cuts at: 1 957 1424 1447 1782 2028 3040 3126 3138
Size: 956 467 23 335 246 1012 86 12
Cuts at: 3138 3484 1
Size: 346 449
Fragments arranged by size:
1012 956 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 218 591 878 218
Size: 373 287 3272
Fragments arranged by size:
3272 373 287
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 741 1882 2063 2226 741
Size: 1141 181 163 2447
Fragments arranged by size:
2447 1141 181 163
BtsI GCAGTG_nn'
Cuts at: 1175 1175
Size: 3932
DraI TTT'AAA
Cuts at: 931 931
Size: 3932
DraIII CAC_nnn'GTG
Cuts at: 324 501 731 324
Size: 177 230 3525
Fragments arranged by size:
3525 230 177
DrdI GACnn_nn'nnGTC
Cuts at: 1638 2496 1638
Size: 858 3074
EaeI y'GGCC_r
Cuts at: 191 221 383 579 1517 1691 2082 2109 3454
Size: 30 162 196 938 174 391 27 1345
Cuts at: 3454 191
Size: 669
Fragments arranged by size:
1345 938 669 391 196 174 162 30
27
EagI C'GGCC_G
Cuts at: 383 579 1517 383
Size: 196 938 2798
Fragments arranged by size:
2798 938 196
EarI CTCTTCn'nnn_
Cuts at: 1955 2165 1955
Size: 210 3722
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 855 2140 2450 2596 3742 855
Size: 1285 310 146 1146 1045
Fragments arranged by size:
1285 1146 1045 310 146
EcoICRI GAG'CTC
Cuts at: 944 944
Size: 3932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1757 2189 2936 3204 1757
Size: 432 747 268 2485
Fragments arranged by size:
2485 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 831 872 1071 831
Size: 41 199 3692
Fragments arranged by size:
3692 199 41
EcoRI G'AATT_C
Cuts at: 936 936
Size: 3932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1951 1951
Size: 3932
FspI TGC'GCA
Cuts at: 458 1713 458
Size: 1255 2677
HaeII r_GCGC'y
Cuts at: 253 473 552 777 825 1366 1614 2636 3615
Size: 220 79 225 48 541 248 1022 979
Cuts at: 3615 253
Size: 570
Fragments arranged by size:
1022 979 570 541 248 225 220 79
48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1774 1804 1806 1836 1774
Size: 30 2 30 3870
Fragments arranged by size:
3870 30 30 2
HincII GTy'rAC
Cuts at: 267 971 267
Size: 704 3228
KasI G'GCGC_C
Cuts at: 469 773 1362 1610 469
Size: 304 589 248 2791
Fragments arranged by size:
2791 589 304 248
KpnI G_GTAC'C
Cuts at: 218 952 218
Size: 734 3198
MfeI C'AATT_G
Cuts at: 158 158
Size: 3932
MluI A'CGCG_T
Cuts at: 638 3365 638
Size: 2727 1205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2603 2787 2603
Size: 184 3748
MscI TGG'CCA
Cuts at: 193 223 1693 193
Size: 30 1470 2432
Fragments arranged by size:
2432 1470 30
MslI CAynn'nnrTG
Cuts at: 229 346 373 1921 2048 229
Size: 117 27 1548 127 2113
Fragments arranged by size:
2113 1548 127 117 27
MspA1I CmG'CkG
Cuts at: 880 1043 1357 1717 2730 2975 880
Size: 163 314 360 1013 245 1837
Fragments arranged by size:
1837 1013 360 314 245 163
NaeI GCC'GGC
Cuts at: 468 762 2113 468
Size: 294 1351 2287
Fragments arranged by size:
2287 1351 294
NarI GG'CG_CC
Cuts at: 470 774 1363 1611 470
Size: 304 589 248 2791
Fragments arranged by size:
2791 589 304 248
NcoI C'CATG_G
Cuts at: 591 591
Size: 3932
NgoMIV G'CCGG_C
Cuts at: 466 760 2111 466
Size: 294 1351 2287
Fragments arranged by size:
2287 1351 294
NotI GC'GGCC_GC
Cuts at: 383 383
Size: 3932
NspI r_CATG'y
Cuts at: 738 985 1485 2392 738
Size: 247 500 907 2278
Fragments arranged by size:
2278 907 500 247
PciI A'CATG_T
Cuts at: 2388 2388
Size: 3932
PmeI GTTT'AAAC
Cuts at: 931 931
Size: 3932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3071 3103 3071
Size: 32 3900
PsiI TTA'TAA
Cuts at: 143 143
Size: 3932
PspXI vC'TCGA_Gb
Cuts at: 808 808
Size: 3932
PstI C_TGCA'G
Cuts at: 854 979 1664 854
Size: 125 685 3122
Fragments arranged by size:
3122 685 125
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3932
PvuII CAG'CTG
Cuts at: 1357 1717 1357
Size: 360 3572
RsrII CG'GwC_CG
Cuts at: 2127 2127
Size: 3932
SacI G_AGCT'C
Cuts at: 946 946
Size: 3932
SacII CC_GC'GG
Cuts at: 881 881
Size: 3932
SalI G'TCGA_C
Cuts at: 969 969
Size: 3932
SapI GCTCTTCn'nnn_
Cuts at: 1955 2165 1955
Size: 210 3722
SbfI CC_TGCA'GG
Cuts at: 979 979
Size: 3932
ScaI AGT'ACT
Cuts at: 3449 3449
Size: 3932
SexAI A'CCwGG_T
Cuts at: 497 497
Size: 3932
SfcI C'TryA_G
Cuts at: 33 850 975 1660 2653 2844 33
Size: 817 125 685 993 191 1121
Fragments arranged by size:
1121 993 817 685 191 125
SfoI GGC'GCC
Cuts at: 471 775 1364 1612 471
Size: 304 589 248 2791
Fragments arranged by size:
2791 589 304 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
SgrAI Cr'CCGG_yG
Cuts at: 466 466
Size: 3932
SmaI CCC'GGG
Cuts at: 954 954
Size: 3932
SmlI C'TyrA_G
Cuts at: 514 808 1087 2002 2494 2756 514
Size: 294 279 915 492 262 1690
Fragments arranged by size:
1690 915 492 294 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3932
SphI G_CATG'C
Cuts at: 738 985 1485 738
Size: 247 500 3185
Fragments arranged by size:
3185 500 247
StuI AGG'CCT
Cuts at: 806 806
Size: 3932
StyI C'CwwG_G
Cuts at: 179 591 1066 1420 179
Size: 412 475 354 2691
Fragments arranged by size:
2691 475 412 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1515 1648 2107 2316 1515
Size: 133 459 209 3131
Fragments arranged by size:
3131 459 209 133
TatI w'GTAC_w
Cuts at: 206 365 3447 206
Size: 159 3082 691
Fragments arranged by size:
3082 691 159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 3900
Tth111I GACn'n_nGTC
Cuts at: 262 1729 262
Size: 1467 2465
XbaI T'CTAG_A
Cuts at: 963 963
Size: 3932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 186 231 1427 186
Size: 45 1196 2691
Fragments arranged by size:
2691 1196 45
XhoI C'TCGA_G
Cuts at: 808 808
Size: 3932
XmaI C'CCGG_G
Cuts at: 952 952
Size: 3932
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI
PmeI PpiI PsiI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BfrBI BglI BmtI BpmI BsaXI
BseRI BsiWI BsmI BsmBI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI NdeI NheI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PsrI SanDI SfiI SnaBI SrfI SspI
SwaI XmnI ZraI