Restriction map for
pF1KB8783
(Circular) (Six-base) MAPSORT of: pF1KB8783.seq Check: 9298 from: 1 to: 3944
pF1KB8783 3944 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 501 501
Size: 3944
AccI GT'mk_AC
Cuts at: 386 982 386
Size: 596 3348
Acc65I G'GTAC_C
Cuts at: 960 960
Size: 3944
AclI AA'CG_TT
Cuts at: 3784 3784
Size: 3944
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1769 2201 2948 3216 322
Size: 1447 432 747 268 1050
Fragments arranged by size:
1447 1050 747 432 268
AflII C'TTAA_G
Cuts at: 759 759
Size: 3944
AflIII A'CryG_T
Cuts at: 1296 2400 3377 1296
Size: 1104 977 1863
Fragments arranged by size:
1863 1104 977
AgeI A'CCGG_T
Cuts at: 2332 2332
Size: 3944
AleI CACnn'nnGTG
Cuts at: 706 721 706
Size: 15 3929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 233 739 233
Size: 506 3438
AlwNI CAG_nnn'CTG
Cuts at: 866 1249 2816 866
Size: 383 1567 1994
Fragments arranged by size:
1994 1567 383
ApaLI G'TGCA_C
Cuts at: 365 2714 365
Size: 2349 1595
ApoI r'AATT_y
Cuts at: 932 948 3297 3553 932
Size: 16 2349 256 1323
Fragments arranged by size:
2349 1323 256 16
AseI AT'TA_AT
Cuts at: 20 20
Size: 3944
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3944
AvaI C'yCGr_G
Cuts at: 117 244 655 964 117
Size: 127 411 309 3097
Fragments arranged by size:
3097 411 309 127
BamHI G'GATC_C
Cuts at: 969 969
Size: 3944
BanI G'GyrC_C
Cuts at: 513 960 1374 1622 1657 513
Size: 447 414 248 35 2800
Fragments arranged by size:
2800 447 414 248 35
BanII G_rGCy'C
Cuts at: 901 958 1988 901
Size: 57 1030 2857
Fragments arranged by size:
2857 1030 57
BbeI G_GCGC'C
Cuts at: 1378 1626 1378
Size: 248 3696
BciVI GTATCCnnnnn_n'
Cuts at: 306 1842 2603 3944 306
Size: 1536 761 1341 306
Fragments arranged by size:
1536 1341 761 306
BclI T'GATC_A
Cuts at: 1464 1464
Size: 3944
BglII A'GATC_T
Cuts at: 1459 3496 1459
Size: 2037 1907
BlpI GC'TnA_GC
Cuts at: 1056 1056
Size: 3944
Bme1580I G_kGCm'C
Cuts at: 369 518 1569 1662 2718 369
Size: 149 1051 93 1056 1595
Fragments arranged by size:
1595 1056 1051 149 93
BmrI ACTGGGnnnn_n'
Cuts at: 746 1156 1237 1572 3716 746
Size: 410 81 335 2144 974
Fragments arranged by size:
2144 974 410 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 3944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 220
Size: 3944
Bpu10I CC'TnA_GC
Cuts at: 791 791
Size: 3944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 757 1120 1999 2491 2789 136
Size: 621 363 879 492 298 1291
Fragments arranged by size:
1291 879 621 492 363 298
BsaI GGTCTCn'nnnn_
Cuts at: 3648 3648
Size: 3944
BsaAI yAC'GTr
Cuts at: 1297 1297
Size: 3944
BsaBI GATnn'nnATC
Cuts at: 6 1458 3495 6
Size: 1452 2037 455
Fragments arranged by size:
2037 1452 455
BsaHI Gr'CG_yC
Cuts at: 1375 1623 2325 3821 1375
Size: 248 702 1496 1498
Fragments arranged by size:
1498 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1133 1654 2332 2606 2753 3164 1133
Size: 521 678 274 147 411 1913
Fragments arranged by size:
1913 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1369 2127 2704 3200 1369
Size: 758 577 496 2113
Fragments arranged by size:
2113 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 699 897 239
Size: 460 198 3286
Fragments arranged by size:
3286 460 198
BsiEI CG_ry'CG
Cuts at: 104 1486 1532 2740 104
Size: 1382 46 1208 1308
Fragments arranged by size:
1382 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 369 958 1736 1926 2718 369
Size: 589 778 190 792 1595
Fragments arranged by size:
1595 792 778 589 190
BsmI GAATG_Cn'
Cuts at: 499 807 499
Size: 308 3636
Bsp1286I G_dGCh'C
Cuts at: 369 518 901 958 1569 1662 1736 1926 1988
Size: 149 383 57 611 93 74 190 62
Cuts at: 1988 2718 369
Size: 730 1595
Fragments arranged by size:
1595 730 611 383 190 149 93 74
62 57
BspEI T'CCGG_A
Cuts at: 3164 3164
Size: 3944
BspHI T'CATG_A
Cuts at: 3120 3120
Size: 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 501 994 1208 1510 1891 501
Size: 493 214 302 381 2554
Fragments arranged by size:
2554 493 381 302 214
BsrBI CCG'CTC
Cuts at: 50 350 747 2236 2290 3774 3939 50
Size: 300 397 1489 54 1484 165 55
Fragments arranged by size:
1489 1484 397 300 165 55 54
BsrDI GCAATG_nn'
Cuts at: 392 1856 392
Size: 1464 2480
BsrFI r'CCGG_y
Cuts at: 1942 2123 2332 1942
Size: 181 209 3554
Fragments arranged by size:
3554 209 181
BssSI C'ACGA_G
Cuts at: 359 2215 2573 359
Size: 1856 358 1730
Fragments arranged by size:
1856 1730 358
BstAPI GCAn_nnn'nTGC
Cuts at: 866 1321 866
Size: 455 3489
BstBI TT'CG_AA
Cuts at: 2305 2305
Size: 3944
BstXI CCAn_nnnn'nTGG
Cuts at: 279 279
Size: 3944
BstYI r'GATC_y
Cuts at: 1 194 969 1436 1459 1794 2040 3052 3138
Size: 193 775 467 23 335 246 1012 86
Cuts at: 3138 3150 3496 1
Size: 12 346 449
Fragments arranged by size:
1012 775 467 449 346 335 246 193
86 23 12
BtgI C'CryG_G
Cuts at: 785 785
Size: 3944
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1894 2075 2238 1894
Size: 181 163 3600
Fragments arranged by size:
3600 181 163
BtsI GCAGTG_nn'
Cuts at: 1187 1187
Size: 3944
ClaI AT'CG_AT
Cuts at: 426 426
Size: 3944
DraI TTT'AAA
Cuts at: 943 943
Size: 3944
DrdI GACnn_nn'nnGTC
Cuts at: 1650 2508 1650
Size: 858 3086
EaeI y'GGCC_r
Cuts at: 285 617 1529 1703 2094 2121 3466 285
Size: 332 912 174 391 27 1345 763
Fragments arranged by size:
1345 912 763 391 332 174 27
EagI C'GGCC_G
Cuts at: 1529 1529
Size: 3944
EarI CTCTTCn'nnn_
Cuts at: 1967 2177 1967
Size: 210 3734
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2152 2462 2608 3754 2152
Size: 310 146 1146 2342
Fragments arranged by size:
2342 1146 310 146
EcoICRI GAG'CTC
Cuts at: 956 956
Size: 3944
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 322 1769 2201 2948 3216 220
Size: 102 1447 432 747 268 948
Fragments arranged by size:
1447 948 747 432 268 102
EcoO109I rG'GnC_Cy
Cuts at: 795 1083 795
Size: 288 3656
EcoRI G'AATT_C
Cuts at: 948 948
Size: 3944
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1963 1963
Size: 3944
FspI TGC'GCA
Cuts at: 1725 1725
Size: 3944
HaeII r_GCGC'y
Cuts at: 116 549 1378 1626 2648 3627 116
Size: 433 829 248 1022 979 433
Fragments arranged by size:
1022 979 829 433 433 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1786 1816 1818 1848 1786
Size: 30 2 30 3882
Fragments arranged by size:
3882 30 30 2
HincII GTy'rAC
Cuts at: 983 983
Size: 3944
KasI G'GCGC_C
Cuts at: 1374 1622 1374
Size: 248 3696
KpnI G_GTAC'C
Cuts at: 964 964
Size: 3944
MluI A'CGCG_T
Cuts at: 3377 3377
Size: 3944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2615 2799 2615
Size: 184 3760
MscI TGG'CCA
Cuts at: 619 1705 619
Size: 1086 2858
MslI CAynn'nnrTG
Cuts at: 706 721 1933 2060 706
Size: 15 1212 127 2590
Fragments arranged by size:
2590 1212 127 15
MspA1I CmG'CkG
Cuts at: 223 291 404 926 1055 1369 1729 2742 2987
Size: 68 113 522 129 314 360 1013 245
Cuts at: 2987 223
Size: 1180
Fragments arranged by size:
1180 1013 522 360 314 245 129 113
68
NaeI GCC'GGC
Cuts at: 2125 2125
Size: 3944
NarI GG'CG_CC
Cuts at: 1375 1623 1375
Size: 248 3696
NcoI C'CATG_G
Cuts at: 785 785
Size: 3944
NgoMIV G'CCGG_C
Cuts at: 2123 2123
Size: 3944
NspI r_CATG'y
Cuts at: 454 997 1497 2404 454
Size: 543 500 907 1994
Fragments arranged by size:
1994 907 543 500
PasI CC'CwG_GG
Cuts at: 518 518
Size: 3944
PciI A'CATG_T
Cuts at: 2400 2400
Size: 3944
PfoI T'CCnGG_A
Cuts at: 197 197
Size: 3944
PmeI GTTT'AAAC
Cuts at: 943 943
Size: 3944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3083 3115 3083
Size: 32 3912
PstI C_TGCA'G
Cuts at: 212 991 1676 212
Size: 779 685 2480
Fragments arranged by size:
2480 779 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3944
PvuII CAG'CTG
Cuts at: 223 926 1369 1729 223
Size: 703 443 360 2438
Fragments arranged by size:
2438 703 443 360
RsrII CG'GwC_CG
Cuts at: 2139 2139
Size: 3944
SacI G_AGCT'C
Cuts at: 958 958
Size: 3944
SalI G'TCGA_C
Cuts at: 981 981
Size: 3944
SapI GCTCTTCn'nnn_
Cuts at: 1967 2177 1967
Size: 210 3734
SbfI CC_TGCA'GG
Cuts at: 212 991 212
Size: 779 3165
ScaI AGT'ACT
Cuts at: 3461 3461
Size: 3944
SfcI C'TryA_G
Cuts at: 33 208 987 1672 2665 2856 33
Size: 175 779 685 993 191 1121
Fragments arranged by size:
1121 993 779 685 191 175
SfoI GGC'GCC
Cuts at: 1376 1624 1376
Size: 248 3696
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3944
SmaI CCC'GGG
Cuts at: 119 966 119
Size: 847 3097
SmlI C'TyrA_G
Cuts at: 151 759 772 1099 2014 2506 2768 151
Size: 608 13 327 915 492 262 1327
Fragments arranged by size:
1327 915 608 492 327 262 13
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3944
SphI G_CATG'C
Cuts at: 997 1497 997
Size: 500 3444
StuI AGG'CCT
Cuts at: 136 535 821 136
Size: 399 286 3259
Fragments arranged by size:
3259 399 286
StyI C'CwwG_G
Cuts at: 785 1078 1432 785
Size: 293 354 3297
Fragments arranged by size:
3297 354 293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 541 918 1527 1660 2119 2328 541
Size: 377 609 133 459 209 2157
Fragments arranged by size:
2157 609 459 377 209 133
TatI w'GTAC_w
Cuts at: 3459 3459
Size: 3944
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 760 792 3461 3493 760
Size: 32 2669 32 1211
Fragments arranged by size:
2669 1211 32 32
Tth111I GACn'n_nGTC
Cuts at: 1741 1741
Size: 3944
XbaI T'CTAG_A
Cuts at: 975 975
Size: 3944
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 276 1439 276
Size: 1163 2781
XmaI C'CCGG_G
Cuts at: 117 964 117
Size: 847 3097
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AhdI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI
BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI