Restriction map for
pF1KB8785
(Circular) (Six-base) MAPSORT of: pF1KB8785.seq Check: 4220 from: 1 to: 3992
pF1KB8785 3992 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 382 382
Size: 3992
AccI GT'mk_AC
Cuts at: 1030 1030
Size: 3992
Acc65I G'GTAC_C
Cuts at: 1008 1008
Size: 3992
AclI AA'CG_TT
Cuts at: 119 3832 119
Size: 3713 279
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 1817 2249 2996 3264 631
Size: 1186 432 747 268 1359
Fragments arranged by size:
1359 1186 747 432 268
AflIII A'CryG_T
Cuts at: 492 1344 2448 3425 492
Size: 852 1104 977 1059
Fragments arranged by size:
1104 1059 977 852
AgeI A'CCGG_T
Cuts at: 2380 2380
Size: 3992
AlwNI CAG_nnn'CTG
Cuts at: 1297 2864 1297
Size: 1567 2425
ApaLI G'TGCA_C
Cuts at: 2762 2762
Size: 3992
ApoI r'AATT_y
Cuts at: 996 3345 3601 996
Size: 2349 256 1387
Fragments arranged by size:
2349 1387 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3992
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
AvaI C'yCGr_G
Cuts at: 296 1012 296
Size: 716 3276
BamHI G'GATC_C
Cuts at: 135 1017 135
Size: 882 3110
BanI G'GyrC_C
Cuts at: 1008 1422 1670 1705 1008
Size: 414 248 35 3295
Fragments arranged by size:
3295 414 248 35
BanII G_rGCy'C
Cuts at: 1006 2036 1006
Size: 1030 2962
BbeI G_GCGC'C
Cuts at: 1426 1674 1426
Size: 248 3744
BbsI GAAGACnn'nnnn_
Cuts at: 848 848
Size: 3992
BbvCI CC'TCA_GC
Cuts at: 851 938 851
Size: 87 3905
BciVI GTATCCnnnnn_n'
Cuts at: 1890 2651 3992 1890
Size: 761 1341 1890
Fragments arranged by size:
1890 1341 761
BclI T'GATC_A
Cuts at: 914 1512 914
Size: 598 3394
BglII A'GATC_T
Cuts at: 1507 3544 1507
Size: 2037 1955
BlpI GC'TnA_GC
Cuts at: 1104 1104
Size: 3992
Bme1580I G_kGCm'C
Cuts at: 533 1617 1710 2766 533
Size: 1084 93 1056 1759
Fragments arranged by size:
1759 1084 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1204 1285 1620 3764 1204
Size: 81 335 2144 1432
Fragments arranged by size:
2144 1432 335 81
BmtI G_CTAG'C
Cuts at: 338 338
Size: 3992
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 3992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 928
Size: 3992
Bpu10I CC'TnA_GC
Cuts at: 851 938 851
Size: 87 3905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1168 2047 2539 2837 1168
Size: 879 492 298 2323
Fragments arranged by size:
2323 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3696 3696
Size: 3992
BsaAI yAC'GTr
Cuts at: 493 1345 493
Size: 852 3140
BsaBI GATnn'nnATC
Cuts at: 6 1506 3543 6
Size: 1500 2037 455
Fragments arranged by size:
2037 1500 455
BsaHI Gr'CG_yC
Cuts at: 1423 1671 2373 3869 1423
Size: 248 702 1496 1546
Fragments arranged by size:
1546 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1181 1702 2380 2654 2801 3212 1181
Size: 521 678 274 147 411 1961
Fragments arranged by size:
1961 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 417 491 494 497 787 868 417
Size: 74 3 3 290 81 3541
Fragments arranged by size:
3541 290 81 74 3 3
BseYI C'CCAG_C
Cuts at: 486 1417 2175 2752 3248 486
Size: 931 758 577 496 1230
Fragments arranged by size:
1230 931 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 857
Size: 3992
BsiEI CG_ry'CG
Cuts at: 104 1534 1580 2788 104
Size: 1430 46 1208 1308
Fragments arranged by size:
1430 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1006 1784 1974 2766 1006
Size: 778 190 792 2232
Fragments arranged by size:
2232 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 533 1006 1617 1710 1784 1974 2036 2766 533
Size: 473 611 93 74 190 62 730 1759
Fragments arranged by size:
1759 730 611 473 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3212 3212
Size: 3992
BspHI T'CATG_A
Cuts at: 3168 3168
Size: 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 382 1042 1256 1558 1939 382
Size: 660 214 302 381 2435
Fragments arranged by size:
2435 660 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2284 2338 3822 3987 50
Size: 2234 54 1484 165 55
Fragments arranged by size:
2234 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 512 1904 512
Size: 1392 2600
BsrFI r'CCGG_y
Cuts at: 1990 2171 2380 1990
Size: 181 209 3602
Fragments arranged by size:
3602 209 181
BsrGI T'GTAC_A
Cuts at: 476 476
Size: 3992
BssSI C'ACGA_G
Cuts at: 2263 2621 2263
Size: 358 3634
BstAPI GCAn_nnn'nTGC
Cuts at: 1369 1369
Size: 3992
BstBI TT'CG_AA
Cuts at: 2353 2353
Size: 3992
BstXI CCAn_nnnn'nTGG
Cuts at: 132 519 132
Size: 387 3605
BstYI r'GATC_y
Cuts at: 1 135 610 1017 1484 1507 1842 2088 3100
Size: 134 475 407 467 23 335 246 1012
Cuts at: 3100 3186 3198 3544 1
Size: 86 12 346 449
Fragments arranged by size:
1012 475 467 449 407 346 335 246
134 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3992
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1942 2123 2286 1942
Size: 181 163 3648
Fragments arranged by size:
3648 181 163
BtsI GCAGTG_nn'
Cuts at: 1235 1235
Size: 3992
DraI TTT'AAA
Cuts at: 991 991
Size: 3992
DrdI GACnn_nn'nnGTC
Cuts at: 1698 2556 1698
Size: 858 3134
EaeI y'GGCC_r
Cuts at: 110 972 1577 1751 2142 2169 3514 110
Size: 862 605 174 391 27 1345 588
Fragments arranged by size:
1345 862 605 588 391 174 27
EagI C'GGCC_G
Cuts at: 1577 1577
Size: 3992
EarI CTCTTCn'nnn_
Cuts at: 764 2015 2225 764
Size: 1251 210 2531
Fragments arranged by size:
2531 1251 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2200 2510 2656 3802 2200
Size: 310 146 1146 2390
Fragments arranged by size:
2390 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1004 1004
Size: 3992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 928 1817 2249 2996 3264 631
Size: 297 889 432 747 268 1359
Fragments arranged by size:
1359 889 747 432 297 268
EcoNI CCTnn'n_nnAGG
Cuts at: 567 567
Size: 3992
EcoO109I rG'GnC_Cy
Cuts at: 861 1131 861
Size: 270 3722
EcoRI G'AATT_C
Cuts at: 996 996
Size: 3992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2011 2011
Size: 3992
FspI TGC'GCA
Cuts at: 1773 1773
Size: 3992
HaeII r_GCGC'y
Cuts at: 1426 1674 2696 3675 1426
Size: 248 1022 979 1743
Fragments arranged by size:
1743 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 123 127 155 159 1834 1864 1866 1896 123
Size: 4 28 4 1675 30 2 30 2219
Fragments arranged by size:
2219 1675 30 30 28 4 4 2
HincII GTy'rAC
Cuts at: 544 668 1031 544
Size: 124 363 3505
Fragments arranged by size:
3505 363 124
KasI G'GCGC_C
Cuts at: 1422 1670 1422
Size: 248 3744
KpnI G_GTAC'C
Cuts at: 1012 1012
Size: 3992
MluI A'CGCG_T
Cuts at: 3425 3425
Size: 3992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2663 2847 2663
Size: 184 3808
MscI TGG'CCA
Cuts at: 112 974 1753 112
Size: 862 779 2351
Fragments arranged by size:
2351 862 779
MslI CAynn'nnrTG
Cuts at: 1981 2108 1981
Size: 127 3865
MspA1I CmG'CkG
Cuts at: 412 906 1103 1417 1777 2790 3035 412
Size: 494 197 314 360 1013 245 1369
Fragments arranged by size:
1369 1013 494 360 314 245 197
NaeI GCC'GGC
Cuts at: 2173 2173
Size: 3992
NarI GG'CG_CC
Cuts at: 1423 1671 1423
Size: 248 3744
NcoI C'CATG_G
Cuts at: 107 107
Size: 3992
NgoMIV G'CCGG_C
Cuts at: 2171 2171
Size: 3992
NheI G'CTAG_C
Cuts at: 334 334
Size: 3992
NspI r_CATG'y
Cuts at: 1045 1545 2452 1045
Size: 500 907 2585
Fragments arranged by size:
2585 907 500
PciI A'CATG_T
Cuts at: 2448 2448
Size: 3992
PfoI T'CCnGG_A
Cuts at: 698 698
Size: 3992
PmeI GTTT'AAAC
Cuts at: 991 991
Size: 3992
PmlI CAC'GTG
Cuts at: 493 493
Size: 3992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3131 3163 3131
Size: 32 3960
PpuMI rG'GwC_Cy
Cuts at: 861 861
Size: 3992
PstI C_TGCA'G
Cuts at: 1039 1724 1039
Size: 685 3307
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3992
PvuII CAG'CTG
Cuts at: 906 1417 1777 906
Size: 511 360 3121
Fragments arranged by size:
3121 511 360
RsrII CG'GwC_CG
Cuts at: 2187 2187
Size: 3992
SacI G_AGCT'C
Cuts at: 1006 1006
Size: 3992
SalI G'TCGA_C
Cuts at: 1029 1029
Size: 3992
SanDI GG'GwC_CC
Cuts at: 861 861
Size: 3992
SapI GCTCTTCn'nnn_
Cuts at: 2015 2225 2015
Size: 210 3782
SbfI CC_TGCA'GG
Cuts at: 1039 1039
Size: 3992
ScaI AGT'ACT
Cuts at: 3509 3509
Size: 3992
SfcI C'TryA_G
Cuts at: 33 305 524 1035 1720 2713 2904 33
Size: 272 219 511 685 993 191 1121
Fragments arranged by size:
1121 993 685 511 272 219 191
SfoI GGC'GCC
Cuts at: 1424 1672 1424
Size: 248 3744
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
SmaI CCC'GGG
Cuts at: 298 1014 298
Size: 716 3276
SmlI C'TyrA_G
Cuts at: 1147 2062 2554 2816 1147
Size: 915 492 262 2323
Fragments arranged by size:
2323 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3992
SphI G_CATG'C
Cuts at: 1045 1545 1045
Size: 500 3492
SrfI GCCC'GGGC
Cuts at: 298 298
Size: 3992
StyI C'CwwG_G
Cuts at: 107 1126 1480 107
Size: 1019 354 2619
Fragments arranged by size:
2619 1019 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1575 1708 2167 2376 1575
Size: 133 459 209 3191
Fragments arranged by size:
3191 459 209 133
TatI w'GTAC_w
Cuts at: 476 3507 476
Size: 3031 961
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 3960
Tth111I GACn'n_nGTC
Cuts at: 1789 1789
Size: 3992
XbaI T'CTAG_A
Cuts at: 1023 1023
Size: 3992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1487 1487
Size: 3992
XmaI C'CCGG_G
Cuts at: 296 1012 296
Size: 716 3276
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PciI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BsaXI
BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PasI PflMI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SspI StuI SwaI XhoI XmnI ZraI