Restriction map for
pF1KB8786
(Circular) (Six-base) MAPSORT of: pF1KB8786.seq Check: 5737 from: 1 to: 4004
pF1KB8786 4004 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1042 1042
Size: 4004
Acc65I G'GTAC_C
Cuts at: 518 1020 518
Size: 502 3502
AclI AA'CG_TT
Cuts at: 3844 3844
Size: 4004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 447 1829 2261 3008 3276 447
Size: 1382 432 747 268 1175
Fragments arranged by size:
1382 1175 747 432 268
AflIII A'CryG_T
Cuts at: 692 869 1356 2460 3437 692
Size: 177 487 1104 977 1259
Fragments arranged by size:
1259 1104 977 487 177
AgeI A'CCGG_T
Cuts at: 2392 2392
Size: 4004
AleI CACnn'nnGTG
Cuts at: 550 601 550
Size: 51 3953
AlwNI CAG_nnn'CTG
Cuts at: 995 1309 2876 995
Size: 314 1567 2123
Fragments arranged by size:
2123 1567 314
ApaI G_GGCC'C
Cuts at: 807 807
Size: 4004
ApaLI G'TGCA_C
Cuts at: 688 2774 688
Size: 2086 1918
ApoI r'AATT_y
Cuts at: 1008 3357 3613 1008
Size: 2349 256 1399
Fragments arranged by size:
2349 1399 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4004
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
AvaI C'yCGr_G
Cuts at: 781 1024 781
Size: 243 3761
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 502 535 502
Size: 33 3971
BamHI G'GATC_C
Cuts at: 1029 1029
Size: 4004
BanI G'GyrC_C
Cuts at: 276 518 1020 1434 1682 1717 276
Size: 242 502 414 248 35 2563
Fragments arranged by size:
2563 502 414 248 242 35
BanII G_rGCy'C
Cuts at: 807 949 956 1018 2048 807
Size: 142 7 62 1030 2763
Fragments arranged by size:
2763 1030 142 62 7
BbeI G_GCGC'C
Cuts at: 1438 1686 1438
Size: 248 3756
BbsI GAAGACnn'nnnn_
Cuts at: 660 780 660
Size: 120 3884
BciVI GTATCCnnnnn_n'
Cuts at: 1902 2663 4004 1902
Size: 761 1341 1902
Fragments arranged by size:
1902 1341 761
BclI T'GATC_A
Cuts at: 356 632 899 1524 356
Size: 276 267 625 2836
Fragments arranged by size:
2836 625 276 267
BglII A'GATC_T
Cuts at: 338 725 1519 3556 338
Size: 387 794 2037 786
Fragments arranged by size:
2037 794 786 387
BlpI GC'TnA_GC
Cuts at: 1116 1116
Size: 4004
Bme1580I G_kGCm'C
Cuts at: 626 692 807 1629 1722 2778 626
Size: 66 115 822 93 1056 1852
Fragments arranged by size:
1852 1056 822 115 93 66
BmgBI CAC'GTC
Cuts at: 644 644
Size: 4004
BmrI ACTGGGnnnn_n'
Cuts at: 1005 1216 1297 1632 3776 1005
Size: 211 81 335 2144 1233
Fragments arranged by size:
2144 1233 335 211 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 4004
Bpu10I CC'TnA_GC
Cuts at: 955 955
Size: 4004
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 1180 2059 2551 2849 367
Size: 813 879 492 298 1522
Fragments arranged by size:
1522 879 813 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3708 3708
Size: 4004
BsaAI yAC'GTr
Cuts at: 391 1357 391
Size: 966 3038
BsaBI GATnn'nnATC
Cuts at: 6 1518 3555 6
Size: 1512 2037 455
Fragments arranged by size:
2037 1512 455
BsaHI Gr'CG_yC
Cuts at: 1435 1683 2385 3881 1435
Size: 248 702 1496 1558
Fragments arranged by size:
1558 1496 702 248
BsaWI w'CCGG_w
Cuts at: 941 1193 1714 2392 2666 2813 3224 941
Size: 252 521 678 274 147 411 1721
Fragments arranged by size:
1721 678 521 411 274 252 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 205
Size: 4004
BseYI C'CCAG_C
Cuts at: 321 800 1429 2187 2764 3260 321
Size: 479 629 758 577 496 1065
Fragments arranged by size:
1065 758 629 577 496 479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 564 564
Size: 4004
BsiEI CG_ry'CG
Cuts at: 104 1546 1592 2800 104
Size: 1442 46 1208 1308
Fragments arranged by size:
1442 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 692 956 1018 1796 1986 2778 692
Size: 264 62 778 190 792 1918
Fragments arranged by size:
1918 792 778 264 190 62
BsmI GAATG_Cn'
Cuts at: 964 964
Size: 4004
Bsp1286I G_dGCh'C
Cuts at: 626 692 807 949 956 1018 1629 1722 1796
Size: 66 115 142 7 62 611 93 74
Cuts at: 1796 1986 2048 2778 626
Size: 190 62 730 1852
Fragments arranged by size:
1852 730 611 190 142 115 93 74
66 62 62 7
BspEI T'CCGG_A
Cuts at: 941 3224 941
Size: 2283 1721
BspHI T'CATG_A
Cuts at: 3180 3180
Size: 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 980 1054 1268 1570 1951 980
Size: 74 214 302 381 3033
Fragments arranged by size:
3033 381 302 214 74
BsrBI CCG'CTC
Cuts at: 50 2296 2350 3834 3999 50
Size: 2246 54 1484 165 55
Fragments arranged by size:
2246 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 915 1916 915
Size: 1001 3003
BsrFI r'CCGG_y
Cuts at: 2002 2183 2392 2002
Size: 181 209 3614
Fragments arranged by size:
3614 209 181
BssSI C'ACGA_G
Cuts at: 173 2275 2633 173
Size: 2102 358 1544
Fragments arranged by size:
2102 1544 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1381 1381
Size: 4004
BstBI TT'CG_AA
Cuts at: 2365 2365
Size: 4004
BstYI r'GATC_y
Cuts at: 1 338 725 1029 1496 1519 1854 2100 3112
Size: 337 387 304 467 23 335 246 1012
Cuts at: 3112 3198 3210 3556 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 387 346 337 335 304
246 86 23 12
BtgI C'CryG_G
Cuts at: 599 599
Size: 4004
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1954 2135 2298 1954
Size: 181 163 3660
Fragments arranged by size:
3660 181 163
BtsI GCAGTG_nn'
Cuts at: 511 1247 511
Size: 736 3268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 372 407 372
Size: 35 3969
DraI TTT'AAA
Cuts at: 1003 1003
Size: 4004
DrdI GACnn_nn'nnGTC
Cuts at: 1710 2568 1710
Size: 858 3146
EaeI y'GGCC_r
Cuts at: 305 1589 1763 2154 2181 3526 305
Size: 1284 174 391 27 1345 783
Fragments arranged by size:
1345 1284 783 391 174 27
EagI C'GGCC_G
Cuts at: 1589 1589
Size: 4004
EarI CTCTTCn'nnn_
Cuts at: 883 2027 2237 883
Size: 1144 210 2650
Fragments arranged by size:
2650 1144 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2212 2522 2668 3814 2212
Size: 310 146 1146 2402
Fragments arranged by size:
2402 1146 310 146
EcoICRI GAG'CTC
Cuts at: 954 1016 954
Size: 62 3942
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 447 1829 2261 3008 3276 256
Size: 191 1382 432 747 268 984
Fragments arranged by size:
1382 984 747 432 268 191
EcoO109I rG'GnC_Cy
Cuts at: 804 1143 804
Size: 339 3665
EcoRI G'AATT_C
Cuts at: 1008 1008
Size: 4004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 996 2023 996
Size: 1027 2977
FspI TGC'GCA
Cuts at: 1785 1785
Size: 4004
HaeII r_GCGC'y
Cuts at: 1438 1686 2708 3687 1438
Size: 248 1022 979 1755
Fragments arranged by size:
1755 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1846 1876 1878 1908 1846
Size: 30 2 30 3942
Fragments arranged by size:
3942 30 30 2
HincII GTy'rAC
Cuts at: 1043 1043
Size: 4004
KasI G'GCGC_C
Cuts at: 1434 1682 1434
Size: 248 3756
KpnI G_GTAC'C
Cuts at: 522 1024 522
Size: 502 3502
MluI A'CGCG_T
Cuts at: 3437 3437
Size: 4004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 188 198 2675 2859 188
Size: 10 2477 184 1333
Fragments arranged by size:
2477 1333 184 10
MscI TGG'CCA
Cuts at: 307 1765 307
Size: 1458 2546
MslI CAynn'nnrTG
Cuts at: 550 598 601 1993 2120 550
Size: 48 3 1392 127 2434
Fragments arranged by size:
2434 1392 127 48 3
MspA1I CmG'CkG
Cuts at: 657 1115 1429 1789 2802 3047 657
Size: 458 314 360 1013 245 1614
Fragments arranged by size:
1614 1013 458 360 314 245
NaeI GCC'GGC
Cuts at: 2185 2185
Size: 4004
NarI GG'CG_CC
Cuts at: 1435 1683 1435
Size: 248 3756
NcoI C'CATG_G
Cuts at: 599 599
Size: 4004
NgoMIV G'CCGG_C
Cuts at: 2183 2183
Size: 4004
NspI r_CATG'y
Cuts at: 696 873 897 1057 1557 2464 696
Size: 177 24 160 500 907 2236
Fragments arranged by size:
2236 907 500 177 160 24
PciI A'CATG_T
Cuts at: 692 869 2460 692
Size: 177 1591 2236
Fragments arranged by size:
2236 1591 177
PmeI GTTT'AAAC
Cuts at: 1003 1003
Size: 4004
PmlI CAC'GTG
Cuts at: 391 391
Size: 4004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 3972
PspOMI G'GGCC_C
Cuts at: 803 803
Size: 4004
PstI C_TGCA'G
Cuts at: 988 1051 1736 988
Size: 63 685 3256
Fragments arranged by size:
3256 685 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4004
PvuII CAG'CTG
Cuts at: 1429 1789 1429
Size: 360 3644
RsrII CG'GwC_CG
Cuts at: 486 2199 486
Size: 1713 2291
SacI G_AGCT'C
Cuts at: 956 1018 956
Size: 62 3942
SalI G'TCGA_C
Cuts at: 1041 1041
Size: 4004
SapI GCTCTTCn'nnn_
Cuts at: 2027 2237 2027
Size: 210 3794
SbfI CC_TGCA'GG
Cuts at: 1051 1051
Size: 4004
ScaI AGT'ACT
Cuts at: 3521 3521
Size: 4004
SfcI C'TryA_G
Cuts at: 33 297 984 1047 1732 2725 2916 33
Size: 264 687 63 685 993 191 1121
Fragments arranged by size:
1121 993 687 685 264 191 63
SfoI GGC'GCC
Cuts at: 1436 1684 1436
Size: 248 3756
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
SmaI CCC'GGG
Cuts at: 1026 1026
Size: 4004
SmlI C'TyrA_G
Cuts at: 382 1159 2074 2566 2828 382
Size: 777 915 492 262 1558
Fragments arranged by size:
1558 915 777 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4004
SphI G_CATG'C
Cuts at: 1057 1557 1057
Size: 500 3504
StyI C'CwwG_G
Cuts at: 599 1138 1492 599
Size: 539 354 3111
Fragments arranged by size:
3111 539 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 294 1587 1720 2179 2388 294
Size: 1293 133 459 209 1910
Fragments arranged by size:
1910 1293 459 209 133
TatI w'GTAC_w
Cuts at: 284 3519 284
Size: 3235 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 758 790 3521 3553 758
Size: 32 2731 32 1209
Fragments arranged by size:
2731 1209 32 32
Tth111I GACn'n_nGTC
Cuts at: 1801 1801
Size: 4004
XbaI T'CTAG_A
Cuts at: 1035 1035
Size: 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1499 1499
Size: 4004
XmaI C'CCGG_G
Cuts at: 1024 1024
Size: 4004
XmnI GAAnn'nnTTC
Cuts at: 159 159
Size: 4004
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PmlI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BbvCI BcgI BfrBI BglI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI