Restriction map for
pF1KB8791
(Circular) (Six-base) MAPSORT of: pF1KB8791.seq Check: 8108 from: 1 to: 4070
pF1KB8791 4070 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1108 1108
Size: 4070
Acc65I G'GTAC_C
Cuts at: 1086 1086
Size: 4070
AclI AA'CG_TT
Cuts at: 3910 3910
Size: 4070
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 1895 2327 3074 3342 225
Size: 1670 432 747 268 953
Fragments arranged by size:
1670 953 747 432 268
AflII C'TTAA_G
Cuts at: 253 253
Size: 4070
AflIII A'CryG_T
Cuts at: 605 620 1422 2526 3503 605
Size: 15 802 1104 977 1172
Fragments arranged by size:
1172 1104 977 802 15
AgeI A'CCGG_T
Cuts at: 461 2458 461
Size: 1997 2073
AleI CACnn'nnGTG
Cuts at: 394 394
Size: 4070
AlwNI CAG_nnn'CTG
Cuts at: 858 1375 2942 858
Size: 517 1567 1986
Fragments arranged by size:
1986 1567 517
ApaI G_GGCC'C
Cuts at: 1011 1011
Size: 4070
ApaLI G'TGCA_C
Cuts at: 387 2840 387
Size: 2453 1617
ApoI r'AATT_y
Cuts at: 988 1074 3423 3679 988
Size: 86 2349 256 1379
Fragments arranged by size:
2349 1379 256 86
AseI AT'TA_AT
Cuts at: 20 20
Size: 4070
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4070
AvaI C'yCGr_G
Cuts at: 1058 1090 1058
Size: 32 4038
BamHI G'GATC_C
Cuts at: 1095 1095
Size: 4070
BanI G'GyrC_C
Cuts at: 1086 1500 1748 1783 1086
Size: 414 248 35 3373
Fragments arranged by size:
3373 414 248 35
BanII G_rGCy'C
Cuts at: 1011 1084 2114 1011
Size: 73 1030 2967
Fragments arranged by size:
2967 1030 73
BbeI G_GCGC'C
Cuts at: 1504 1752 1504
Size: 248 3822
BbsI GAAGACnn'nnnn_
Cuts at: 426 642 801 426
Size: 216 159 3695
Fragments arranged by size:
3695 216 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 259 293 259
Size: 34 4036
BciVI GTATCCnnnnn_n'
Cuts at: 1968 2729 4070 1968
Size: 761 1341 1968
Fragments arranged by size:
1968 1341 761
BclI T'GATC_A
Cuts at: 296 1590 296
Size: 1294 2776
BglI GCCn_nnn'nGGC
Cuts at: 802 802
Size: 4070
BglII A'GATC_T
Cuts at: 518 1585 3622 518
Size: 1067 2037 966
Fragments arranged by size:
2037 1067 966
BlpI GC'TnA_GC
Cuts at: 1182 1182
Size: 4070
Bme1580I G_kGCm'C
Cuts at: 391 1011 1695 1788 2844 391
Size: 620 684 93 1056 1617
Fragments arranged by size:
1617 1056 684 620 93
BmrI ACTGGGnnnn_n'
Cuts at: 768 823 1282 1363 1698 3842 768
Size: 55 459 81 335 2144 996
Fragments arranged by size:
2144 996 459 335 81 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 4070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 657 682 696 657
Size: 25 14 4031
Fragments arranged by size:
4031 25 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 2125 2617 2915 1246
Size: 879 492 298 2401
Fragments arranged by size:
2401 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 737 822 3774 737
Size: 85 2952 1033
Fragments arranged by size:
2952 1033 85
BsaAI yAC'GTr
Cuts at: 1423 1423
Size: 4070
BsaBI GATnn'nnATC
Cuts at: 6 1584 3621 6
Size: 1578 2037 455
Fragments arranged by size:
2037 1578 455
BsaHI Gr'CG_yC
Cuts at: 1501 1749 2451 3947 1501
Size: 248 702 1496 1624
Fragments arranged by size:
1624 1496 702 248
BsaWI w'CCGG_w
Cuts at: 461 503 559 1259 1780 2458 2732 2879 3290
Size: 42 56 700 521 678 274 147 411
Cuts at: 3290 461
Size: 1241
Fragments arranged by size:
1241 700 678 521 411 274 147 56
42
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 205
Size: 4070
BseYI C'CCAG_C
Cuts at: 732 1495 2253 2830 3326 732
Size: 763 758 577 496 1476
Fragments arranged by size:
1476 763 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 784 906 519
Size: 265 122 3683
Fragments arranged by size:
3683 265 122
BsiEI CG_ry'CG
Cuts at: 104 1612 1658 2866 104
Size: 1508 46 1208 1308
Fragments arranged by size:
1508 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 332 391 1084 1862 2052 2844 332
Size: 59 693 778 190 792 1558
Fragments arranged by size:
1558 792 778 693 190 59
Bsp1286I G_dGCh'C
Cuts at: 332 391 1011 1084 1695 1788 1862 2052 2114
Size: 59 620 73 611 93 74 190 62
Cuts at: 2114 2844 332
Size: 730 1558
Fragments arranged by size:
1558 730 620 611 190 93 74 73
62 59
BspEI T'CCGG_A
Cuts at: 3290 3290
Size: 4070
BspHI T'CATG_A
Cuts at: 3246 3246
Size: 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 230 326 1120 1334 1636 2017 230
Size: 96 794 214 302 381 2283
Fragments arranged by size:
2283 794 381 302 214 96
BsrBI CCG'CTC
Cuts at: 50 486 2362 2416 3900 4065 50
Size: 436 1876 54 1484 165 55
Fragments arranged by size:
1876 1484 436 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1982 1982
Size: 4070
BsrFI r'CCGG_y
Cuts at: 461 1013 2068 2249 2458 461
Size: 552 1055 181 209 2073
Fragments arranged by size:
2073 1055 552 209 181
BssSI C'ACGA_G
Cuts at: 2341 2699 2341
Size: 358 3712
BstAPI GCAn_nnn'nTGC
Cuts at: 1447 1447
Size: 4070
BstBI TT'CG_AA
Cuts at: 2431 2431
Size: 4070
BstYI r'GATC_y
Cuts at: 1 518 527 1095 1562 1585 1920 2166 3178
Size: 517 9 568 467 23 335 246 1012
Cuts at: 3178 3264 3276 3622 1
Size: 86 12 346 449
Fragments arranged by size:
1012 568 517 467 449 346 335 246
86 23 12 9
BtgI C'CryG_G
Cuts at: 234 585 686 234
Size: 351 101 3618
Fragments arranged by size:
3618 351 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 774 2020 2201 2364 774
Size: 1246 181 163 2480
Fragments arranged by size:
2480 1246 181 163
BtsI GCAGTG_nn'
Cuts at: 1313 1313
Size: 4070
DraI TTT'AAA
Cuts at: 1069 1069
Size: 4070
DrdI GACnn_nn'nnGTC
Cuts at: 1776 2634 1776
Size: 858 3212
EaeI y'GGCC_r
Cuts at: 227 470 1655 1829 2220 2247 3592 227
Size: 243 1185 174 391 27 1345 705
Fragments arranged by size:
1345 1185 705 391 243 174 27
EagI C'GGCC_G
Cuts at: 1655 1655
Size: 4070
EarI CTCTTCn'nnn_
Cuts at: 2093 2303 2093
Size: 210 3860
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2278 2588 2734 3880 2278
Size: 310 146 1146 2468
Fragments arranged by size:
2468 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1082 1082
Size: 4070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 657 682 696 1895 2327 3074 3342 225
Size: 432 25 14 1199 432 747 268 953
Fragments arranged by size:
1199 953 747 432 432 268 25 14
EcoNI CCTnn'n_nnAGG
Cuts at: 358 358
Size: 4070
EcoO109I rG'GnC_Cy
Cuts at: 546 1007 1209 546
Size: 461 202 3407
Fragments arranged by size:
3407 461 202
EcoRI G'AATT_C
Cuts at: 988 1074 988
Size: 86 3984
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 669 2089 669
Size: 1420 2650
FspI TGC'GCA
Cuts at: 1851 1851
Size: 4070
HaeII r_GCGC'y
Cuts at: 644 1504 1752 2774 3753 644
Size: 860 248 1022 979 961
Fragments arranged by size:
1022 979 961 860 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1912 1942 1944 1974 1912
Size: 30 2 30 4008
Fragments arranged by size:
4008 30 30 2
HincII GTy'rAC
Cuts at: 153 945 1109 153
Size: 792 164 3114
Fragments arranged by size:
3114 792 164
KasI G'GCGC_C
Cuts at: 1500 1748 1500
Size: 248 3822
KpnI G_GTAC'C
Cuts at: 1090 1090
Size: 4070
MfeI C'AATT_G
Cuts at: 919 919
Size: 4070
MluI A'CGCG_T
Cuts at: 3503 3503
Size: 4070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2741 2925 2741
Size: 184 3886
MscI TGG'CCA
Cuts at: 229 472 1831 229
Size: 243 1359 2468
Fragments arranged by size:
2468 1359 243
MslI CAynn'nnrTG
Cuts at: 292 394 454 2059 2186 292
Size: 102 60 1605 127 2176
Fragments arranged by size:
2176 1605 127 102 60
MspA1I CmG'CkG
Cuts at: 660 1032 1181 1495 1855 2868 3113 660
Size: 372 149 314 360 1013 245 1617
Fragments arranged by size:
1617 1013 372 360 314 245 149
NaeI GCC'GGC
Cuts at: 2251 2251
Size: 4070
NarI GG'CG_CC
Cuts at: 1501 1749 1501
Size: 248 3822
NcoI C'CATG_G
Cuts at: 234 585 686 234
Size: 351 101 3618
Fragments arranged by size:
3618 351 101
NgoMIV G'CCGG_C
Cuts at: 2249 2249
Size: 4070
NspI r_CATG'y
Cuts at: 423 609 624 1123 1623 2530 423
Size: 186 15 499 500 907 1963
Fragments arranged by size:
1963 907 500 499 186 15
PciI A'CATG_T
Cuts at: 605 620 2526 605
Size: 15 1906 2149
Fragments arranged by size:
2149 1906 15
PfoI T'CCnGG_A
Cuts at: 712 712
Size: 4070
PmeI GTTT'AAAC
Cuts at: 1069 1069
Size: 4070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 4038
PpuMI rG'GwC_Cy
Cuts at: 546 546
Size: 4070
PspOMI G'GGCC_C
Cuts at: 1007 1007
Size: 4070
PstI C_TGCA'G
Cuts at: 854 1043 1117 1802 854
Size: 189 74 685 3122
Fragments arranged by size:
3122 685 189 74
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4070
PvuII CAG'CTG
Cuts at: 1032 1495 1855 1032
Size: 463 360 3247
Fragments arranged by size:
3247 463 360
RsrII CG'GwC_CG
Cuts at: 2265 2265
Size: 4070
SacI G_AGCT'C
Cuts at: 1084 1084
Size: 4070
SalI G'TCGA_C
Cuts at: 1107 1107
Size: 4070
SapI GCTCTTCn'nnn_
Cuts at: 2093 2303 2093
Size: 210 3860
SbfI CC_TGCA'GG
Cuts at: 1043 1117 1043
Size: 74 3996
ScaI AGT'ACT
Cuts at: 3587 3587
Size: 4070
SfcI C'TryA_G
Cuts at: 33 172 576 783 850 1039 1113 1798 2791
Size: 139 404 207 67 189 74 685 993
Cuts at: 2791 2982 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 404 207 191 189 139
74 67
SfoI GGC'GCC
Cuts at: 1502 1750 1502
Size: 248 3822
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4070
SmaI CCC'GGG
Cuts at: 1060 1092 1060
Size: 32 4038
SmlI C'TyrA_G
Cuts at: 253 1225 2140 2632 2894 253
Size: 972 915 492 262 1429
Fragments arranged by size:
1429 972 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4070
SphI G_CATG'C
Cuts at: 1123 1623 1123
Size: 500 3570
StyI C'CwwG_G
Cuts at: 234 585 644 686 1204 1558 234
Size: 351 59 42 518 354 2746
Fragments arranged by size:
2746 518 354 351 59 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 750 766 1653 1786 2245 2454 750
Size: 16 887 133 459 209 2366
Fragments arranged by size:
2366 887 459 209 133 16
TatI w'GTAC_w
Cuts at: 3585 3585
Size: 4070
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 490 522 1003 1035 3587 3619 490
Size: 32 481 32 2552 32 941
Fragments arranged by size:
2552 941 481 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 148 1867 148
Size: 1719 2351
XbaI T'CTAG_A
Cuts at: 1101 1101
Size: 4070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 201 546 693 1029 1565 201
Size: 345 147 336 536 2706
Fragments arranged by size:
2706 536 345 336 147
XmaI C'CCGG_G
Cuts at: 1058 1090 1058
Size: 32 4038
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI