Restriction map for
pF1KB8792
(Circular) (Six-base) MAPSORT of: pF1KB8792.seq Check: 668 from: 1 to: 4094
pF1KB8792 4094 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1132 1132
Size: 4094
Acc65I G'GTAC_C
Cuts at: 1110 1110
Size: 4094
AclI AA'CG_TT
Cuts at: 3934 3934
Size: 4094
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1919 2351 3098 3366 1919
Size: 432 747 268 2647
Fragments arranged by size:
2647 747 432 268
AflIII A'CryG_T
Cuts at: 1446 2550 3527 1446
Size: 1104 977 2013
Fragments arranged by size:
2013 1104 977
AgeI A'CCGG_T
Cuts at: 2482 2482
Size: 4094
AleI CACnn'nnGTG
Cuts at: 275 359 610 862 946 275
Size: 84 251 252 84 3423
Fragments arranged by size:
3423 252 251 84 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 134 134
Size: 4094
AlwNI CAG_nnn'CTG
Cuts at: 148 1399 2966 148
Size: 1251 1567 1276
Fragments arranged by size:
1567 1276 1251
ApaLI G'TGCA_C
Cuts at: 410 745 2864 410
Size: 335 2119 1640
Fragments arranged by size:
2119 1640 335
ApoI r'AATT_y
Cuts at: 266 434 517 601 769 853 937 1098 3447
Size: 168 83 84 168 84 84 161 2349
Cuts at: 3447 3703 266
Size: 256 657
Fragments arranged by size:
2349 657 256 168 168 161 84 84
84 83
AseI AT'TA_AT
Cuts at: 20 20
Size: 4094
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
AvaI C'yCGr_G
Cuts at: 1114 1114
Size: 4094
BamHI G'GATC_C
Cuts at: 1119 1119
Size: 4094
BanI G'GyrC_C
Cuts at: 1110 1524 1772 1807 1110
Size: 414 248 35 3397
Fragments arranged by size:
3397 414 248 35
BanII G_rGCy'C
Cuts at: 1108 2138 1108
Size: 1030 3064
BbeI G_GCGC'C
Cuts at: 1528 1776 1528
Size: 248 3846
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4060
BciVI GTATCCnnnnn_n'
Cuts at: 1992 2753 4094 1992
Size: 761 1341 1992
Fragments arranged by size:
1992 1341 761
BclI T'GATC_A
Cuts at: 1614 1614
Size: 4094
BglII A'GATC_T
Cuts at: 1609 3646 1609
Size: 2037 2057
BlpI GC'TnA_GC
Cuts at: 1206 1206
Size: 4094
Bme1580I G_kGCm'C
Cuts at: 246 414 749 1719 1812 2868 246
Size: 168 335 970 93 1056 1472
Fragments arranged by size:
1472 1056 970 335 168 93
BmrI ACTGGGnnnn_n'
Cuts at: 1306 1387 1722 3866 1306
Size: 81 335 2144 1534
Fragments arranged by size:
2144 1534 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 4094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 2149 2641 2939 1270
Size: 879 492 298 2425
Fragments arranged by size:
2425 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3798 3798
Size: 4094
BsaAI yAC'GTr
Cuts at: 1447 1447
Size: 4094
BsaBI GATnn'nnATC
Cuts at: 6 1608 3645 6
Size: 1602 2037 455
Fragments arranged by size:
2037 1602 455
BsaHI Gr'CG_yC
Cuts at: 1525 1773 2475 3971 1525
Size: 248 702 1496 1648
Fragments arranged by size:
1648 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1283 1804 2482 2756 2903 3314 1283
Size: 521 678 274 147 411 2063
Fragments arranged by size:
2063 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1519 2277 2854 3350 1519
Size: 758 577 496 2263
Fragments arranged by size:
2263 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1636 1682 2890 104
Size: 1532 46 1208 1308
Fragments arranged by size:
1532 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 414 749 1108 1886 2076 2868 414
Size: 335 359 778 190 792 1640
Fragments arranged by size:
1640 792 778 359 335 190
BsmI GAATG_Cn'
Cuts at: 298 648 298
Size: 350 3744
Bsp1286I G_dGCh'C
Cuts at: 246 414 749 1108 1719 1812 1886 2076 2138
Size: 168 335 359 611 93 74 190 62
Cuts at: 2138 2868 246
Size: 730 1472
Fragments arranged by size:
1472 730 611 359 335 190 168 93
74 62
BspEI T'CCGG_A
Cuts at: 3314 3314
Size: 4094
BspHI T'CATG_A
Cuts at: 3270 3270
Size: 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1144 1358 1660 2041 1144
Size: 214 302 381 3197
Fragments arranged by size:
3197 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2386 2440 3924 4089 50
Size: 2336 54 1484 165 55
Fragments arranged by size:
2336 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 128 475 2006 128
Size: 347 1531 2216
Fragments arranged by size:
2216 1531 347
BsrFI r'CCGG_y
Cuts at: 2092 2273 2482 2092
Size: 181 209 3704
Fragments arranged by size:
3704 209 181
BsrGI T'GTAC_A
Cuts at: 546 546
Size: 4094
BssSI C'ACGA_G
Cuts at: 2365 2723 2365
Size: 358 3736
BstAPI GCAn_nnn'nTGC
Cuts at: 148 1471 148
Size: 1323 2771
BstBI TT'CG_AA
Cuts at: 2455 2455
Size: 4094
BstYI r'GATC_y
Cuts at: 1 1119 1586 1609 1944 2190 3202 3288 3300
Size: 1118 467 23 335 246 1012 86 12
Cuts at: 3300 3646 1
Size: 346 449
Fragments arranged by size:
1118 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2044 2225 2388 2044
Size: 181 163 3750
Fragments arranged by size:
3750 181 163
BtsI GCAGTG_nn'
Cuts at: 978 1337 978
Size: 359 3735
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 285 320 285
Size: 35 4059
DraI TTT'AAA
Cuts at: 131 1093 131
Size: 962 3132
DrdI GACnn_nn'nnGTC
Cuts at: 1800 2658 1800
Size: 858 3236
EaeI y'GGCC_r
Cuts at: 1679 1853 2244 2271 3616 1679
Size: 174 391 27 1345 2157
Fragments arranged by size:
2157 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1679 1679
Size: 4094
EarI CTCTTCn'nnn_
Cuts at: 2117 2327 2117
Size: 210 3884
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 420 2302 2612 2758 3904 420
Size: 1882 310 146 1146 610
Fragments arranged by size:
1882 1146 610 310 146
EcoICRI GAG'CTC
Cuts at: 1106 1106
Size: 4094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1919 2351 3098 3366 1919
Size: 432 747 268 2647
Fragments arranged by size:
2647 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1233 1233
Size: 4094
EcoRI G'AATT_C
Cuts at: 266 434 601 769 853 937 1098 266
Size: 168 167 168 84 84 161 3262
Fragments arranged by size:
3262 168 168 167 161 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1085 2113 1085
Size: 1028 3066
FspI TGC'GCA
Cuts at: 1875 1875
Size: 4094
HaeII r_GCGC'y
Cuts at: 1528 1776 2798 3777 1528
Size: 248 1022 979 1845
Fragments arranged by size:
1845 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 399 431 1936 1966 1968 1998 399
Size: 32 1505 30 2 30 2495
Fragments arranged by size:
2495 1505 32 30 30 2
HincII GTy'rAC
Cuts at: 1133 1133
Size: 4094
KasI G'GCGC_C
Cuts at: 1524 1772 1524
Size: 248 3846
KpnI G_GTAC'C
Cuts at: 1114 1114
Size: 4094
MfeI C'AATT_G
Cuts at: 908 908
Size: 4094
MluI A'CGCG_T
Cuts at: 3527 3527
Size: 4094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 454 1023 2765 2949 454
Size: 569 1742 184 1599
Fragments arranged by size:
1742 1599 569 184
MscI TGG'CCA
Cuts at: 1855 1855
Size: 4094
MslI CAynn'nnrTG
Cuts at: 275 291 359 443 526 610 694 778 794
Size: 16 68 84 83 84 84 84 16
Cuts at: 794 862 946 1030 2083 2210 275
Size: 68 84 84 1053 127 2159
Fragments arranged by size:
2159 1053 127 84 84 84 84 84
84 83 68 68 16 16
MspA1I CmG'CkG
Cuts at: 1205 1519 1879 2892 3137 1205
Size: 314 360 1013 245 2162
Fragments arranged by size:
2162 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2275 2275
Size: 4094
NarI GG'CG_CC
Cuts at: 1525 1773 1525
Size: 248 3846
NdeI CA'TA_TG
Cuts at: 288 791 288
Size: 503 3591
NgoMIV G'CCGG_C
Cuts at: 2273 2273
Size: 4094
NspI r_CATG'y
Cuts at: 1147 1647 2554 1147
Size: 500 907 2687
Fragments arranged by size:
2687 907 500
PciI A'CATG_T
Cuts at: 2550 2550
Size: 4094
PmeI GTTT'AAAC
Cuts at: 1093 1093
Size: 4094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 4062
PsiI TTA'TAA
Cuts at: 458 458
Size: 4094
PstI C_TGCA'G
Cuts at: 1141 1826 1141
Size: 685 3409
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4094
PvuII CAG'CTG
Cuts at: 1519 1879 1519
Size: 360 3734
RsrII CG'GwC_CG
Cuts at: 2289 2289
Size: 4094
SacI G_AGCT'C
Cuts at: 1108 1108
Size: 4094
SalI G'TCGA_C
Cuts at: 1131 1131
Size: 4094
SapI GCTCTTCn'nnn_
Cuts at: 2117 2327 2117
Size: 210 3884
SbfI CC_TGCA'GG
Cuts at: 1141 1141
Size: 4094
ScaI AGT'ACT
Cuts at: 3611 3611
Size: 4094
SfcI C'TryA_G
Cuts at: 33 1137 1822 2815 3006 33
Size: 1104 685 993 191 1121
Fragments arranged by size:
1121 1104 993 685 191
SfoI GGC'GCC
Cuts at: 1526 1774 1526
Size: 248 3846
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
SmaI CCC'GGG
Cuts at: 1116 1116
Size: 4094
SmlI C'TyrA_G
Cuts at: 1249 2164 2656 2918 1249
Size: 915 492 262 2425
Fragments arranged by size:
2425 915 492 262
SpeI A'CTAG_T
Cuts at: 67 358 67
Size: 291 3803
SphI G_CATG'C
Cuts at: 1147 1647 1147
Size: 500 3594
StuI AGG'CCT
Cuts at: 481 1068 481
Size: 587 3507
StyI C'CwwG_G
Cuts at: 1228 1582 1228
Size: 354 3740
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1677 1810 2269 2478 1677
Size: 133 459 209 3293
Fragments arranged by size:
3293 459 209 133
TatI w'GTAC_w
Cuts at: 546 3609 546
Size: 3063 1031
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3611 3643 3611
Size: 32 4062
Tth111I GACn'n_nGTC
Cuts at: 1891 1891
Size: 4094
XbaI T'CTAG_A
Cuts at: 1125 1125
Size: 4094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1589 1589
Size: 4094
XmaI C'CCGG_G
Cuts at: 1114 1114
Size: 4094
XmnI GAAnn'nnTTC
Cuts at: 816 1068 816
Size: 252 3842
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NcoI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI