Restriction map for
pF1KB8793
(Circular) (Six-base) MAPSORT of: pF1KB8793.seq Check: 9777 from: 1 to: 4103
pF1KB8793 4103 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 305 1141 305
Size: 836 3267
Acc65I G'GTAC_C
Cuts at: 1119 1119
Size: 4103
AclI AA'CG_TT
Cuts at: 3943 3943
Size: 4103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1928 2360 3107 3375 1928
Size: 432 747 268 2656
Fragments arranged by size:
2656 747 432 268
AfeI AGC'GCT
Cuts at: 487 487
Size: 4103
AflIII A'CryG_T
Cuts at: 1455 2559 3536 1455
Size: 1104 977 2022
Fragments arranged by size:
2022 1104 977
AgeI A'CCGG_T
Cuts at: 2491 2491
Size: 4103
AleI CACnn'nnGTG
Cuts at: 427 427
Size: 4103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1043 1043
Size: 4103
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 589 621 589
Size: 32 4071
AlwNI CAG_nnn'CTG
Cuts at: 1408 2975 1408
Size: 1567 2536
ApaI G_GGCC'C
Cuts at: 573 573
Size: 4103
ApaLI G'TGCA_C
Cuts at: 2873 2873
Size: 4103
ApoI r'AATT_y
Cuts at: 1107 3456 3712 1107
Size: 2349 256 1498
Fragments arranged by size:
2349 1498 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4103
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
AvaI C'yCGr_G
Cuts at: 1123 1123
Size: 4103
BamHI G'GATC_C
Cuts at: 111 731 1128 111
Size: 620 397 3086
Fragments arranged by size:
3086 620 397
BanI G'GyrC_C
Cuts at: 1066 1119 1533 1781 1816 1066
Size: 53 414 248 35 3353
Fragments arranged by size:
3353 414 248 53 35
BanII G_rGCy'C
Cuts at: 573 958 1017 1117 2147 573
Size: 385 59 100 1030 2529
Fragments arranged by size:
2529 1030 385 100 59
BbeI G_GCGC'C
Cuts at: 1537 1785 1537
Size: 248 3855
BbsI GAAGACnn'nnnn_
Cuts at: 201 201
Size: 4103
BbvCI CC'TCA_GC
Cuts at: 313 1031 313
Size: 718 3385
BciVI GTATCCnnnnn_n'
Cuts at: 2001 2762 4103 2001
Size: 761 1341 2001
Fragments arranged by size:
2001 1341 761
BclI T'GATC_A
Cuts at: 1623 1623
Size: 4103
BglII A'GATC_T
Cuts at: 1618 3655 1618
Size: 2037 2066
BlpI GC'TnA_GC
Cuts at: 466 586 1215 466
Size: 120 629 3354
Fragments arranged by size:
3354 629 120
Bme1580I G_kGCm'C
Cuts at: 573 1069 1728 1821 2877 573
Size: 496 659 93 1056 1799
Fragments arranged by size:
1799 1056 659 496 93
BmrI ACTGGGnnnn_n'
Cuts at: 1315 1396 1731 3875 1315
Size: 81 335 2144 1543
Fragments arranged by size:
2144 1543 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 729 991 729
Size: 262 3841
Bpu10I CC'TnA_GC
Cuts at: 313 457 1031 313
Size: 144 574 3385
Fragments arranged by size:
3385 574 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 1279 2158 2650 2948 979
Size: 300 879 492 298 2134
Fragments arranged by size:
2134 879 492 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 187 3807 187
Size: 3620 483
BsaAI yAC'GTr
Cuts at: 1456 1456
Size: 4103
BsaBI GATnn'nnATC
Cuts at: 6 1617 3654 6
Size: 1611 2037 455
Fragments arranged by size:
2037 1611 455
BsaHI Gr'CG_yC
Cuts at: 1534 1782 2484 3980 1534
Size: 248 702 1496 1657
Fragments arranged by size:
1657 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1292 1813 2491 2765 2912 3323 1292
Size: 521 678 274 147 411 2072
Fragments arranged by size:
2072 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 796 796
Size: 4103
BseYI C'CCAG_C
Cuts at: 1528 2286 2863 3359 1528
Size: 758 577 496 2272
Fragments arranged by size:
2272 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 551 1645 1691 2899 104
Size: 447 1094 46 1208 1308
Fragments arranged by size:
1308 1208 1094 447 46
BsiHKAI G_wGCw'C
Cuts at: 263 472 1117 1895 2085 2877 263
Size: 209 645 778 190 792 1489
Fragments arranged by size:
1489 792 778 645 209 190
BsmI GAATG_Cn'
Cuts at: 827 827
Size: 4103
Bsp1286I G_dGCh'C
Cuts at: 263 472 573 958 1017 1069 1117 1728 1821
Size: 209 101 385 59 52 48 611 93
Cuts at: 1821 1895 2085 2147 2877 263
Size: 74 190 62 730 1489
Fragments arranged by size:
1489 730 611 385 209 190 101 93
74 62 59 52 48
BspEI T'CCGG_A
Cuts at: 3323 3323
Size: 4103
BspHI T'CATG_A
Cuts at: 3279 3279
Size: 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1153 1367 1669 2050 1153
Size: 214 302 381 3206
Fragments arranged by size:
3206 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2395 2449 3933 4098 50
Size: 2345 54 1484 165 55
Fragments arranged by size:
2345 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 224 1025 2015 224
Size: 801 990 2312
Fragments arranged by size:
2312 990 801
BsrFI r'CCGG_y
Cuts at: 2101 2282 2491 2101
Size: 181 209 3713
Fragments arranged by size:
3713 209 181
BssSI C'ACGA_G
Cuts at: 2374 2732 2374
Size: 358 3745
BstAPI GCAn_nnn'nTGC
Cuts at: 1480 1480
Size: 4103
BstBI TT'CG_AA
Cuts at: 2464 2464
Size: 4103
BstYI r'GATC_y
Cuts at: 1 111 731 851 1128 1595 1618 1953 2199
Size: 110 620 120 277 467 23 335 246
Cuts at: 2199 3211 3297 3309 3655 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 620 467 449 346 335 277 246
120 110 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2053 2234 2397 2053
Size: 181 163 3759
Fragments arranged by size:
3759 181 163
BtsI GCAGTG_nn'
Cuts at: 665 930 976 1346 665
Size: 265 46 370 3422
Fragments arranged by size:
3422 370 265 46
DraI TTT'AAA
Cuts at: 1102 1102
Size: 4103
DrdI GACnn_nn'nnGTC
Cuts at: 1809 2667 1809
Size: 858 3245
EaeI y'GGCC_r
Cuts at: 320 1688 1862 2253 2280 3625 320
Size: 1368 174 391 27 1345 798
Fragments arranged by size:
1368 1345 798 391 174 27
EagI C'GGCC_G
Cuts at: 1688 1688
Size: 4103
EarI CTCTTCn'nnn_
Cuts at: 343 2126 2336 343
Size: 1783 210 2110
Fragments arranged by size:
2110 1783 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2311 2621 2767 3913 2311
Size: 310 146 1146 2501
Fragments arranged by size:
2501 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1115 1115
Size: 4103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 729 991 1928 2360 3107 3375 729
Size: 262 937 432 747 268 1457
Fragments arranged by size:
1457 937 747 432 268 262
EcoO109I rG'GnC_Cy
Cuts at: 570 1242 570
Size: 672 3431
EcoRI G'AATT_C
Cuts at: 1107 1107
Size: 4103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 190 2122 190
Size: 1932 2171
FspI TGC'GCA
Cuts at: 286 1884 286
Size: 1598 2505
HaeII r_GCGC'y
Cuts at: 489 1537 1785 2807 3786 489
Size: 1048 248 1022 979 806
Fragments arranged by size:
1048 1022 979 806 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 619 651 1074 1106 1945 1975 1977 2007 619
Size: 32 423 32 839 30 2 30 2715
Fragments arranged by size:
2715 839 423 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1142 1142
Size: 4103
KasI G'GCGC_C
Cuts at: 1533 1781 1533
Size: 248 3855
KpnI G_GTAC'C
Cuts at: 1123 1123
Size: 4103
MluI A'CGCG_T
Cuts at: 3536 3536
Size: 4103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 139 2774 2958 139
Size: 2635 184 1284
Fragments arranged by size:
2635 1284 184
MscI TGG'CCA
Cuts at: 1864 1864
Size: 4103
MslI CAynn'nnrTG
Cuts at: 427 430 2092 2219 427
Size: 3 1662 127 2311
Fragments arranged by size:
2311 1662 127 3
MspA1I CmG'CkG
Cuts at: 547 1214 1528 1888 2901 3146 547
Size: 667 314 360 1013 245 1504
Fragments arranged by size:
1504 1013 667 360 314 245
NaeI GCC'GGC
Cuts at: 2284 2284
Size: 4103
NarI GG'CG_CC
Cuts at: 1534 1782 1534
Size: 248 3855
NcoI C'CATG_G
Cuts at: 107 107
Size: 4103
NgoMIV G'CCGG_C
Cuts at: 2282 2282
Size: 4103
NspI r_CATG'y
Cuts at: 285 465 1156 1656 2563 285
Size: 180 691 500 907 1825
Fragments arranged by size:
1825 907 691 500 180
PciI A'CATG_T
Cuts at: 2559 2559
Size: 4103
PmeI GTTT'AAAC
Cuts at: 1102 1102
Size: 4103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 283 315 3242 3274 283
Size: 32 2927 32 1112
Fragments arranged by size:
2927 1112 32 32
PshAI GACnn'nnGTC
Cuts at: 540 540
Size: 4103
PsiI TTA'TAA
Cuts at: 358 358
Size: 4103
PspOMI G'GGCC_C
Cuts at: 569 569
Size: 4103
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 126 158 126
Size: 32 4071
PstI C_TGCA'G
Cuts at: 672 983 1022 1150 1835 672
Size: 311 39 128 685 2940
Fragments arranged by size:
2940 685 311 128 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4103
PvuII CAG'CTG
Cuts at: 1528 1888 1528
Size: 360 3743
RsrII CG'GwC_CG
Cuts at: 2298 2298
Size: 4103
SacI G_AGCT'C
Cuts at: 1117 1117
Size: 4103
SalI G'TCGA_C
Cuts at: 1140 1140
Size: 4103
SapI GCTCTTCn'nnn_
Cuts at: 2126 2336 2126
Size: 210 3893
SbfI CC_TGCA'GG
Cuts at: 1150 1150
Size: 4103
ScaI AGT'ACT
Cuts at: 3620 3620
Size: 4103
SfcI C'TryA_G
Cuts at: 33 668 979 1018 1146 1831 2824 3015 33
Size: 635 311 39 128 685 993 191 1121
Fragments arranged by size:
1121 993 685 635 311 191 128 39
SfoI GGC'GCC
Cuts at: 1535 1783 1535
Size: 248 3855
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
SmaI CCC'GGG
Cuts at: 1125 1125
Size: 4103
SmlI C'TyrA_G
Cuts at: 994 1258 2173 2665 2927 994
Size: 264 915 492 262 2170
Fragments arranged by size:
2170 915 492 264 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4103
SphI G_CATG'C
Cuts at: 285 465 1156 1656 285
Size: 180 691 500 2732
Fragments arranged by size:
2732 691 500 180
SspI AAT'ATT
Cuts at: 845 845
Size: 4103
StuI AGG'CCT
Cuts at: 455 455
Size: 4103
StyI C'CwwG_G
Cuts at: 107 1237 1591 107
Size: 1130 354 2619
Fragments arranged by size:
2619 1130 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1686 1819 2278 2487 1686
Size: 133 459 209 3302
Fragments arranged by size:
3302 459 209 133
TatI w'GTAC_w
Cuts at: 142 3618 142
Size: 3476 627
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 594 626 723 755 3620 3652 594
Size: 32 97 32 2865 32 1045
Fragments arranged by size:
2865 1045 97 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1900 1900
Size: 4103
XbaI T'CTAG_A
Cuts at: 1134 1134
Size: 4103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1598 1598
Size: 4103
XmaI C'CCGG_G
Cuts at: 1123 1123
Size: 4103
XmnI GAAnn'nnTTC
Cuts at: 295 295
Size: 4103
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AscI AvrII BaeI BcgI
BfrBI BglI BmgBI BmtI BsaXI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI