Restriction map for
pF1KB8794
(Circular) (Six-base) MAPSORT of: pF1KB8794.seq Check: 4334 from: 1 to: 4103
pF1KB8794 4103 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 812 812
Size: 4103
AccI GT'mk_AC
Cuts at: 486 503 902 1141 486
Size: 17 399 239 3448
Fragments arranged by size:
3448 399 239 17
Acc65I G'GTAC_C
Cuts at: 1119 1119
Size: 4103
AclI AA'CG_TT
Cuts at: 3943 3943
Size: 4103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 1928 2360 3107 3375 778
Size: 1150 432 747 268 1506
Fragments arranged by size:
1506 1150 747 432 268
AflIII A'CryG_T
Cuts at: 655 1455 2559 3536 655
Size: 800 1104 977 1222
Fragments arranged by size:
1222 1104 977 800
AgeI A'CCGG_T
Cuts at: 2491 2491
Size: 4103
AleI CACnn'nnGTG
Cuts at: 265 265
Size: 4103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 416 751 416
Size: 335 3768
AlwNI CAG_nnn'CTG
Cuts at: 1408 2975 1408
Size: 1567 2536
ApaI G_GGCC'C
Cuts at: 184 197 184
Size: 13 4090
ApaLI G'TGCA_C
Cuts at: 2873 2873
Size: 4103
ApoI r'AATT_y
Cuts at: 1107 3456 3712 1107
Size: 2349 256 1498
Fragments arranged by size:
2349 1498 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4103
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
AvaI C'yCGr_G
Cuts at: 1123 1123
Size: 4103
BamHI G'GATC_C
Cuts at: 1128 1128
Size: 4103
BanI G'GyrC_C
Cuts at: 259 595 800 1119 1533 1781 1816 259
Size: 336 205 319 414 248 35 2546
Fragments arranged by size:
2546 414 336 319 248 205 35
BanII G_rGCy'C
Cuts at: 184 197 1117 2147 184
Size: 13 920 1030 2140
Fragments arranged by size:
2140 1030 920 13
BbeI G_GCGC'C
Cuts at: 1537 1785 1537
Size: 248 3855
BbsI GAAGACnn'nnnn_
Cuts at: 399 399
Size: 4103
BciVI GTATCCnnnnn_n'
Cuts at: 2001 2762 4103 2001
Size: 761 1341 2001
Fragments arranged by size:
2001 1341 761
BclI T'GATC_A
Cuts at: 980 1623 980
Size: 643 3460
BglI GCCn_nnn'nGGC
Cuts at: 644 853 644
Size: 209 3894
BglII A'GATC_T
Cuts at: 1618 3655 1618
Size: 2037 2066
BlpI GC'TnA_GC
Cuts at: 420 1215 420
Size: 795 3308
Bme1580I G_kGCm'C
Cuts at: 184 197 310 327 600 1728 1821 2877 184
Size: 13 113 17 273 1128 93 1056 1410
Fragments arranged by size:
1410 1128 1056 273 113 93 17 13
BmrI ACTGGGnnnn_n'
Cuts at: 1082 1315 1396 1731 3875 1082
Size: 233 81 335 2144 1310
Fragments arranged by size:
2144 1310 335 233 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4103
Bpu10I CC'TnA_GC
Cuts at: 153 153
Size: 4103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1279 2158 2650 2948 1279
Size: 879 492 298 2434
Fragments arranged by size:
2434 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3807 3807
Size: 4103
BsaAI yAC'GTr
Cuts at: 1456 1456
Size: 4103
BsaBI GATnn'nnATC
Cuts at: 6 1617 3654 6
Size: 1611 2037 455
Fragments arranged by size:
2037 1611 455
BsaHI Gr'CG_yC
Cuts at: 1534 1782 2484 3980 1534
Size: 248 702 1496 1657
Fragments arranged by size:
1657 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1292 1813 2491 2765 2912 3323 1292
Size: 521 678 274 147 411 2072
Fragments arranged by size:
2072 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 183 779 912 1528 2286 2863 3359 183
Size: 596 133 616 758 577 496 927
Fragments arranged by size:
927 758 616 596 577 496 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 376 1039 140
Size: 236 663 3204
Fragments arranged by size:
3204 663 236
BsiEI CG_ry'CG
Cuts at: 104 507 1645 1691 2899 104
Size: 403 1138 46 1208 1308
Fragments arranged by size:
1308 1208 1138 403 46
BsiHKAI G_wGCw'C
Cuts at: 248 1117 1895 2085 2877 248
Size: 869 778 190 792 1474
Fragments arranged by size:
1474 869 792 778 190
BsmI GAATG_Cn'
Cuts at: 285 285
Size: 4103
Bsp1286I G_dGCh'C
Cuts at: 184 197 248 310 327 600 1117 1728 1821
Size: 13 51 62 17 273 517 611 93
Cuts at: 1821 1895 2085 2147 2877 184
Size: 74 190 62 730 1410
Fragments arranged by size:
1410 730 611 517 273 190 93 74
62 62 51 17 13
BspEI T'CCGG_A
Cuts at: 3323 3323
Size: 4103
BspHI T'CATG_A
Cuts at: 3279 3279
Size: 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 374 812 1153 1367 1669 2050 374
Size: 438 341 214 302 381 2427
Fragments arranged by size:
2427 438 381 341 302 214
BsrBI CCG'CTC
Cuts at: 50 1082 2395 2449 3933 4098 50
Size: 1032 1313 54 1484 165 55
Fragments arranged by size:
1484 1313 1032 165 55 54
BsrDI GCAATG_nn'
Cuts at: 464 704 842 2015 464
Size: 240 138 1173 2552
Fragments arranged by size:
2552 1173 240 138
BsrFI r'CCGG_y
Cuts at: 2101 2282 2491 2101
Size: 181 209 3713
Fragments arranged by size:
3713 209 181
BsrGI T'GTAC_A
Cuts at: 521 521
Size: 4103
BssSI C'ACGA_G
Cuts at: 2374 2732 2374
Size: 358 3745
BstAPI GCAn_nnn'nTGC
Cuts at: 767 807 1026 1480 767
Size: 40 219 454 3390
Fragments arranged by size:
3390 454 219 40
BstBI TT'CG_AA
Cuts at: 2464 2464
Size: 4103
BstXI CCAn_nnnn'nTGG
Cuts at: 303 303
Size: 4103
BstYI r'GATC_y
Cuts at: 1 1064 1128 1595 1618 1953 2199 3211 3297
Size: 1063 64 467 23 335 246 1012 86
Cuts at: 3297 3309 3655 1
Size: 12 346 449
Fragments arranged by size:
1063 1012 467 449 346 335 246 86
64 23 12
Bsu36I CC'TnA_GG
Cuts at: 227 227
Size: 4103
BtgI C'CryG_G
Cuts at: 356 857 356
Size: 501 3602
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2053 2234 2397 2053
Size: 181 163 3759
Fragments arranged by size:
3759 181 163
BtsI GCAGTG_nn'
Cuts at: 214 1346 214
Size: 1132 2971
DraI TTT'AAA
Cuts at: 1102 1102
Size: 4103
DraIII CAC_nnn'GTG
Cuts at: 825 825
Size: 4103
DrdI GACnn_nn'nnGTC
Cuts at: 1809 2667 1809
Size: 858 3245
EaeI y'GGCC_r
Cuts at: 293 353 491 701 827 854 1688 1862 2253
Size: 60 138 210 126 27 834 174 391
Cuts at: 2253 2280 3625 293
Size: 27 1345 771
Fragments arranged by size:
1345 834 771 391 210 174 138 126
60 27 27
EagI C'GGCC_G
Cuts at: 1688 1688
Size: 4103
EarI CTCTTCn'nnn_
Cuts at: 1062 2126 2336 1062
Size: 1064 210 2829
Fragments arranged by size:
2829 1064 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2311 2621 2767 3913 2311
Size: 310 146 1146 2501
Fragments arranged by size:
2501 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1115 1115
Size: 4103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 1928 2360 3107 3375 778
Size: 1150 432 747 268 1506
Fragments arranged by size:
1506 1150 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 193 1242 193
Size: 1049 3054
EcoRI G'AATT_C
Cuts at: 1107 1107
Size: 4103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1086 2122 1086
Size: 1036 3067
FspI TGC'GCA
Cuts at: 1884 1884
Size: 4103
HaeII r_GCGC'y
Cuts at: 1537 1785 2807 3786 1537
Size: 248 1022 979 1854
Fragments arranged by size:
1854 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 910 942 1945 1975 1977 2007 910
Size: 32 1003 30 2 30 3006
Fragments arranged by size:
3006 1003 32 30 30 2
HincII GTy'rAC
Cuts at: 504 1142 504
Size: 638 3465
KasI G'GCGC_C
Cuts at: 1533 1781 1533
Size: 248 3855
KpnI G_GTAC'C
Cuts at: 1123 1123
Size: 4103
MluI A'CGCG_T
Cuts at: 3536 3536
Size: 4103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2774 2958 2774
Size: 184 3919
MscI TGG'CCA
Cuts at: 295 493 703 829 1864 295
Size: 198 210 126 1035 2534
Fragments arranged by size:
2534 1035 210 198 126
MslI CAynn'nnrTG
Cuts at: 265 301 2092 2219 265
Size: 36 1791 127 2149
Fragments arranged by size:
2149 1791 127 36
MspA1I CmG'CkG
Cuts at: 148 424 798 1214 1528 1888 2901 3146 148
Size: 276 374 416 314 360 1013 245 1105
Fragments arranged by size:
1105 1013 416 374 360 314 276 245
NaeI GCC'GGC
Cuts at: 2284 2284
Size: 4103
NarI GG'CG_CC
Cuts at: 1534 1782 1534
Size: 248 3855
NgoMIV G'CCGG_C
Cuts at: 2282 2282
Size: 4103
NspI r_CATG'y
Cuts at: 659 1156 1656 2563 659
Size: 497 500 907 2199
Fragments arranged by size:
2199 907 500 497
PasI CC'CwG_GG
Cuts at: 600 600
Size: 4103
PciI A'CATG_T
Cuts at: 655 2559 655
Size: 1904 2199
PflMI CCAn_nnn'nTGG
Cuts at: 113 825 113
Size: 712 3391
PmeI GTTT'AAAC
Cuts at: 1102 1102
Size: 4103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 4071
PspOMI G'GGCC_C
Cuts at: 180 193 180
Size: 13 4090
PstI C_TGCA'G
Cuts at: 740 1150 1835 740
Size: 410 685 3008
Fragments arranged by size:
3008 685 410
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4103
PvuII CAG'CTG
Cuts at: 148 1528 1888 148
Size: 1380 360 2363
Fragments arranged by size:
2363 1380 360
RsrII CG'GwC_CG
Cuts at: 2298 2298
Size: 4103
SacI G_AGCT'C
Cuts at: 1117 1117
Size: 4103
SalI G'TCGA_C
Cuts at: 502 1140 502
Size: 638 3465
SapI GCTCTTCn'nnn_
Cuts at: 2126 2336 2126
Size: 210 3893
SbfI CC_TGCA'GG
Cuts at: 1150 1150
Size: 4103
ScaI AGT'ACT
Cuts at: 3620 3620
Size: 4103
SfcI C'TryA_G
Cuts at: 33 736 1146 1831 2824 3015 33
Size: 703 410 685 993 191 1121
Fragments arranged by size:
1121 993 703 685 410 191
SfoI GGC'GCC
Cuts at: 1535 1783 1535
Size: 248 3855
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
SmaI CCC'GGG
Cuts at: 1125 1125
Size: 4103
SmlI C'TyrA_G
Cuts at: 1258 2173 2665 2927 1258
Size: 915 492 262 2434
Fragments arranged by size:
2434 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4103
SphI G_CATG'C
Cuts at: 1156 1656 1156
Size: 500 3603
StuI AGG'CCT
Cuts at: 232 377 232
Size: 145 3958
StyI C'CwwG_G
Cuts at: 1237 1591 1237
Size: 354 3749
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 904 1686 1819 2278 2487 904
Size: 782 133 459 209 2520
Fragments arranged by size:
2520 782 459 209 133
TatI w'GTAC_w
Cuts at: 521 3618 521
Size: 3097 1006
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 4071
Tth111I GACn'n_nGTC
Cuts at: 1900 1900
Size: 4103
XbaI T'CTAG_A
Cuts at: 1134 1134
Size: 4103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 336 660 687 855 1598 336
Size: 324 27 168 743 2841
Fragments arranged by size:
2841 743 324 168 27
XmaI C'CCGG_G
Cuts at: 1123 1123
Size: 4103
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BpmI BsaXI BseRI
BsiWI BsmBI BssHII BstEII BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI