Restriction map for
pF1KB8795
(Circular) (Six-base) MAPSORT of: pF1KB8795.seq Check: 6498 from: 1 to: 4106
pF1KB8795 4106 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 1144 1144
Size: 4106
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4106
AclI AA'CG_TT
Cuts at: 3946 3946
Size: 4106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 939 1931 2363 3110 3378 436
Size: 503 992 432 747 268 1164
Fragments arranged by size:
1164 992 747 503 432 268
AflII C'TTAA_G
Cuts at: 792 792
Size: 4106
AflIII A'CryG_T
Cuts at: 700 1458 2562 3539 700
Size: 758 1104 977 1267
Fragments arranged by size:
1267 1104 977 758
AgeI A'CCGG_T
Cuts at: 2494 2494
Size: 4106
AlwNI CAG_nnn'CTG
Cuts at: 1411 2978 1411
Size: 1567 2539
ApaLI G'TGCA_C
Cuts at: 2876 2876
Size: 4106
ApoI r'AATT_y
Cuts at: 126 737 1110 3459 3715 126
Size: 611 373 2349 256 517
Fragments arranged by size:
2349 611 517 373 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 1126 1126
Size: 4106
BamHI G'GATC_C
Cuts at: 1131 1131
Size: 4106
BanI G'GyrC_C
Cuts at: 476 1122 1536 1784 1819 476
Size: 646 414 248 35 2763
Fragments arranged by size:
2763 646 414 248 35
BanII G_rGCy'C
Cuts at: 1120 2150 1120
Size: 1030 3076
BbeI G_GCGC'C
Cuts at: 1540 1788 1540
Size: 248 3858
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2765 4106 2004
Size: 761 1341 2004
Fragments arranged by size:
2004 1341 761
BclI T'GATC_A
Cuts at: 894 1626 894
Size: 732 3374
BglII A'GATC_T
Cuts at: 1621 3658 1621
Size: 2037 2069
BlpI GC'TnA_GC
Cuts at: 1218 1218
Size: 4106
Bme1580I G_kGCm'C
Cuts at: 1731 1824 2880 1731
Size: 93 1056 2957
Fragments arranged by size:
2957 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1318 1399 1734 3878 1318
Size: 81 335 2144 1546
Fragments arranged by size:
2144 1546 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 767 935 767
Size: 168 3938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 953 1005 1282 2161 2653 2951 953
Size: 52 277 879 492 298 2108
Fragments arranged by size:
2108 879 492 298 277 52
BsaI GGTCTCn'nnnn_
Cuts at: 3810 3810
Size: 4106
BsaAI yAC'GTr
Cuts at: 1459 1459
Size: 4106
BsaBI GATnn'nnATC
Cuts at: 6 1620 3657 6
Size: 1614 2037 455
Fragments arranged by size:
2037 1614 455
BsaHI Gr'CG_yC
Cuts at: 1537 1785 2487 3983 1537
Size: 248 702 1496 1660
Fragments arranged by size:
1660 1496 702 248
BsaWI w'CCGG_w
Cuts at: 577 1295 1816 2494 2768 2915 3326 577
Size: 718 521 678 274 147 411 1357
Fragments arranged by size:
1357 718 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 1099 126
Size: 973 3133
BseYI C'CCAG_C
Cuts at: 1531 2289 2866 3362 1531
Size: 758 577 496 2275
Fragments arranged by size:
2275 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1648 1694 2902 104
Size: 1544 46 1208 1308
Fragments arranged by size:
1544 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1120 1898 2088 2880 1120
Size: 778 190 792 2346
Fragments arranged by size:
2346 792 778 190
BsmI GAATG_Cn'
Cuts at: 659 659
Size: 4106
Bsp1286I G_dGCh'C
Cuts at: 1120 1731 1824 1898 2088 2150 2880 1120
Size: 611 93 74 190 62 730 2346
Fragments arranged by size:
2346 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3326 3326
Size: 4106
BspHI T'CATG_A
Cuts at: 3282 3282
Size: 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1156 1370 1672 2053 1156
Size: 214 302 381 3209
Fragments arranged by size:
3209 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2398 2452 3936 4101 50
Size: 2348 54 1484 165 55
Fragments arranged by size:
2348 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2018 2018
Size: 4106
BsrFI r'CCGG_y
Cuts at: 2104 2285 2494 2104
Size: 181 209 3716
Fragments arranged by size:
3716 209 181
BssSI C'ACGA_G
Cuts at: 2377 2735 2377
Size: 358 3748
BstAPI GCAn_nnn'nTGC
Cuts at: 1483 1483
Size: 4106
BstBI TT'CG_AA
Cuts at: 2467 2467
Size: 4106
BstYI r'GATC_y
Cuts at: 1 1131 1598 1621 1956 2202 3214 3300 3312
Size: 1130 467 23 335 246 1012 86 12
Cuts at: 3312 3658 1
Size: 346 449
Fragments arranged by size:
1130 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2056 2237 2400 2056
Size: 181 163 3762
Fragments arranged by size:
3762 181 163
BtsI GCAGTG_nn'
Cuts at: 438 1349 438
Size: 911 3195
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4106
DrdI GACnn_nn'nnGTC
Cuts at: 1812 2670 1812
Size: 858 3248
EaeI y'GGCC_r
Cuts at: 1691 1865 2256 2283 3628 1691
Size: 174 391 27 1345 2169
Fragments arranged by size:
2169 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1691 1691
Size: 4106
EarI CTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2314 2624 2770 3916 2314
Size: 310 146 1146 2504
Fragments arranged by size:
2504 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1118 1118
Size: 4106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 767 935 939 1931 2363 3110 3378 436
Size: 331 168 4 992 432 747 268 1164
Fragments arranged by size:
1164 992 747 432 331 268 168 4
EcoO109I rG'GnC_Cy
Cuts at: 1245 1245
Size: 4106
EcoRI G'AATT_C
Cuts at: 737 1110 737
Size: 373 3733
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 4106
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4106
HaeII r_GCGC'y
Cuts at: 1540 1788 2810 3789 1540
Size: 248 1022 979 1857
Fragments arranged by size:
1857 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 169 201 810 842 1948 1978 1980 2010 169
Size: 32 609 32 1106 30 2 30 2265
Fragments arranged by size:
2265 1106 609 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1145 1145
Size: 4106
HindIII A'AGCT_T
Cuts at: 362 362
Size: 4106
KasI G'GCGC_C
Cuts at: 1536 1784 1536
Size: 248 3858
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4106
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 520 2777 2961 520
Size: 2257 184 1665
Fragments arranged by size:
2257 1665 184
MscI TGG'CCA
Cuts at: 1867 1867
Size: 4106
MslI CAynn'nnrTG
Cuts at: 2095 2222 2095
Size: 127 3979
MspA1I CmG'CkG
Cuts at: 1217 1531 1891 2904 3149 1217
Size: 314 360 1013 245 2174
Fragments arranged by size:
2174 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2287 2287
Size: 4106
NarI GG'CG_CC
Cuts at: 1537 1785 1537
Size: 248 3858
NgoMIV G'CCGG_C
Cuts at: 2285 2285
Size: 4106
NspI r_CATG'y
Cuts at: 431 704 1159 1659 2566 431
Size: 273 455 500 907 1971
Fragments arranged by size:
1971 907 500 455 273
PciI A'CATG_T
Cuts at: 700 2562 700
Size: 1862 2244
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 4074
PshAI GACnn'nnGTC
Cuts at: 200 200
Size: 4106
PstI C_TGCA'G
Cuts at: 1153 1838 1153
Size: 685 3421
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 1531 1891 1531
Size: 360 3746
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 1120 1120
Size: 4106
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SapI GCTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4106
ScaI AGT'ACT
Cuts at: 161 3623 161
Size: 3462 644
SfcI C'TryA_G
Cuts at: 33 1149 1834 2827 3018 33
Size: 1116 685 993 191 1121
Fragments arranged by size:
1121 1116 993 685 191
SfoI GGC'GCC
Cuts at: 1538 1786 1538
Size: 248 3858
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SmaI CCC'GGG
Cuts at: 1128 1128
Size: 4106
SmlI C'TyrA_G
Cuts at: 792 968 984 1261 2176 2668 2930 792
Size: 176 16 277 915 492 262 1968
Fragments arranged by size:
1968 915 492 277 262 176 16
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 1159 1659 1159
Size: 500 3606
StyI C'CwwG_G
Cuts at: 1240 1594 1240
Size: 354 3752
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1689 1822 2281 2490 1689
Size: 133 459 209 3305
Fragments arranged by size:
3305 459 209 133
TatI w'GTAC_w
Cuts at: 159 262 306 3621 159
Size: 103 44 3315 644
Fragments arranged by size:
3315 644 103 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 687 719 3623 3655 687
Size: 32 2904 32 1138
Fragments arranged by size:
2904 1138 32 32
Tth111I GACn'n_nGTC
Cuts at: 1903 1903
Size: 4106
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1601 1601
Size: 4106
XmaI C'CCGG_G
Cuts at: 1126 1126
Size: 4106
XmnI GAAnn'nnTTC
Cuts at: 448 952 448
Size: 504 3602
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI