Restriction map for
pF1KB8796
(Circular) (Six-base) MAPSORT of: pF1KB8796.seq Check: 7747 from: 1 to: 4112
pF1KB8796 4112 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 668 1150 668
Size: 482 3630
Acc65I G'GTAC_C
Cuts at: 653 1128 653
Size: 475 3637
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1937 2369 3116 3384 142
Size: 1795 432 747 268 870
Fragments arranged by size:
1795 870 747 432 268
AflIII A'CryG_T
Cuts at: 1005 1464 2568 3545 1005
Size: 459 1104 977 1572
Fragments arranged by size:
1572 1104 977 459
AgeI A'CCGG_T
Cuts at: 2500 2500
Size: 4112
AlwNI CAG_nnn'CTG
Cuts at: 855 1417 2984 855
Size: 562 1567 1983
Fragments arranged by size:
1983 1567 562
ApaLI G'TGCA_C
Cuts at: 2882 2882
Size: 4112
ApoI r'AATT_y
Cuts at: 1116 3465 3721 1116
Size: 2349 256 1507
Fragments arranged by size:
2349 1507 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 1132 1132
Size: 4112
BamHI G'GATC_C
Cuts at: 1137 1137
Size: 4112
BanI G'GyrC_C
Cuts at: 653 764 1041 1075 1093 1128 1542 1790 1825
Size: 111 277 34 18 35 414 248 35
Cuts at: 1825 653
Size: 2940
Fragments arranged by size:
2940 414 277 248 111 35 35 34
18
BanII G_rGCy'C
Cuts at: 508 1126 2156 508
Size: 618 1030 2464
Fragments arranged by size:
2464 1030 618
BbeI G_GCGC'C
Cuts at: 768 1097 1546 1794 768
Size: 329 449 248 3086
Fragments arranged by size:
3086 449 329 248
BbsI GAAGACnn'nnnn_
Cuts at: 963 963
Size: 4112
BbvCI CC'TCA_GC
Cuts at: 238 814 238
Size: 576 3536
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 1632 1632
Size: 4112
BglI GCCn_nnn'nGGC
Cuts at: 248 248
Size: 4112
BglII A'GATC_T
Cuts at: 1627 3664 1627
Size: 2037 2075
BlpI GC'TnA_GC
Cuts at: 1224 1224
Size: 4112
Bme1580I G_kGCm'C
Cuts at: 1737 1830 2886 1737
Size: 93 1056 2963
Fragments arranged by size:
2963 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 393 1324 1405 1740 3884 393
Size: 931 81 335 2144 621
Fragments arranged by size:
2144 931 621 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 424 1113 2479 424
Size: 689 1366 2057
Fragments arranged by size:
2057 1366 689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 313
Size: 4112
Bpu10I CC'TnA_GC
Cuts at: 238 814 238
Size: 576 3536
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 2167 2659 2957 1288
Size: 879 492 298 2443
Fragments arranged by size:
2443 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3816 3816
Size: 4112
BsaAI yAC'GTr
Cuts at: 650 723 1465 650
Size: 73 742 3297
Fragments arranged by size:
3297 742 73
BsaBI GATnn'nnATC
Cuts at: 6 1626 3663 6
Size: 1620 2037 455
Fragments arranged by size:
2037 1620 455
BsaHI Gr'CG_yC
Cuts at: 765 1094 1543 1791 2493 3989 765
Size: 329 449 248 702 1496 888
Fragments arranged by size:
1496 888 702 449 329 248
BsaWI w'CCGG_w
Cuts at: 875 1301 1822 2500 2774 2921 3332 875
Size: 426 521 678 274 147 411 1655
Fragments arranged by size:
1655 678 521 426 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 646 676 995 1025 646
Size: 30 319 30 3733
Fragments arranged by size:
3733 319 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 409 226
Size: 183 3929
BseYI C'CCAG_C
Cuts at: 389 396 818 1045 1050 1537 2295 2872 3368
Size: 7 422 227 5 487 758 577 496
Cuts at: 3368 389
Size: 1133
Fragments arranged by size:
1133 758 577 496 487 422 227 7
5
BsiEI CG_ry'CG
Cuts at: 104 1654 1700 2908 104
Size: 1550 46 1208 1308
Fragments arranged by size:
1550 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 136 965 1126 1904 2094 2886 136
Size: 829 161 778 190 792 1362
Fragments arranged by size:
1362 829 792 778 190 161
Bsp1286I G_dGCh'C
Cuts at: 136 508 965 1126 1737 1830 1904 2094 2156
Size: 372 457 161 611 93 74 190 62
Cuts at: 2156 2886 136
Size: 730 1362
Fragments arranged by size:
1362 730 611 457 372 190 161 93
74 62
BspEI T'CCGG_A
Cuts at: 875 3332 875
Size: 2457 1655
BspHI T'CATG_A
Cuts at: 473 3288 473
Size: 2815 1297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1162 1376 1678 2059 1162
Size: 214 302 381 3215
Fragments arranged by size:
3215 381 302 214
BsrBI CCG'CTC
Cuts at: 50 749 2404 2458 3942 4107 50
Size: 699 1655 54 1484 165 55
Fragments arranged by size:
1655 1484 699 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2024 2024
Size: 4112
BsrFI r'CCGG_y
Cuts at: 1031 2110 2291 2500 1031
Size: 1079 181 209 2643
Fragments arranged by size:
2643 1079 209 181
BssSI C'ACGA_G
Cuts at: 2383 2741 2383
Size: 358 3754
BstAPI GCAn_nnn'nTGC
Cuts at: 1489 1489
Size: 4112
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstEII G'GTnAC_C
Cuts at: 552 552
Size: 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 383 801 1072 383
Size: 418 271 3423
Fragments arranged by size:
3423 418 271
BstYI r'GATC_y
Cuts at: 1 368 728 1137 1604 1627 1962 2208 3220
Size: 367 360 409 467 23 335 246 1012
Cuts at: 3220 3306 3318 3664 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 409 367 360 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 117 733 117
Size: 616 3496
BtgI C'CryG_G
Cuts at: 107 557 107
Size: 450 3662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2062 2243 2406 2062
Size: 181 163 3768
Fragments arranged by size:
3768 181 163
BtsI GCAGTG_nn'
Cuts at: 942 1355 942
Size: 413 3699
DraI TTT'AAA
Cuts at: 1111 1111
Size: 4112
DraIII CAC_nnn'GTG
Cuts at: 615 615
Size: 4112
DrdI GACnn_nn'nnGTC
Cuts at: 1818 2676 1818
Size: 858 3254
EaeI y'GGCC_r
Cuts at: 1697 1871 2262 2289 3634 1697
Size: 174 391 27 1345 2175
Fragments arranged by size:
2175 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1697 1697
Size: 4112
EarI CTCTTCn'nnn_
Cuts at: 688 2135 2345 688
Size: 1447 210 2455
Fragments arranged by size:
2455 1447 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2320 2630 2776 3922 2320
Size: 310 146 1146 2510
Fragments arranged by size:
2510 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1124 1124
Size: 4112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 313 1937 2369 3116 3384 142
Size: 171 1624 432 747 268 870
Fragments arranged by size:
1624 870 747 432 268 171
EcoO109I rG'GnC_Cy
Cuts at: 270 737 823 1251 270
Size: 467 86 428 3131
Fragments arranged by size:
3131 467 428 86
EcoRI G'AATT_C
Cuts at: 1116 1116
Size: 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 804 2131 804
Size: 1327 2785
FspI TGC'GCA
Cuts at: 1893 1893
Size: 4112
HaeII r_GCGC'y
Cuts at: 768 1097 1546 1794 2816 3795 768
Size: 329 449 248 1022 979 1085
Fragments arranged by size:
1085 1022 979 449 329 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1954 1984 1986 2016 1954
Size: 30 2 30 4050
Fragments arranged by size:
4050 30 30 2
HincII GTy'rAC
Cuts at: 1151 1151
Size: 4112
KasI G'GCGC_C
Cuts at: 764 1093 1542 1790 764
Size: 329 449 248 3086
Fragments arranged by size:
3086 449 329 248
KpnI G_GTAC'C
Cuts at: 657 1132 657
Size: 475 3637
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2783 2967 2783
Size: 184 3928
MscI TGG'CCA
Cuts at: 1873 1873
Size: 4112
MslI CAynn'nnrTG
Cuts at: 562 799 2101 2228 562
Size: 237 1302 127 2446
Fragments arranged by size:
2446 1302 237 127
MspA1I CmG'CkG
Cuts at: 539 818 1223 1537 1897 2910 3155 539
Size: 279 405 314 360 1013 245 1496
Fragments arranged by size:
1496 1013 405 360 314 279 245
NaeI GCC'GGC
Cuts at: 1033 2293 1033
Size: 1260 2852
NarI GG'CG_CC
Cuts at: 765 1094 1543 1791 765
Size: 329 449 248 3086
Fragments arranged by size:
3086 449 329 248
NcoI C'CATG_G
Cuts at: 107 557 107
Size: 450 3662
NgoMIV G'CCGG_C
Cuts at: 1031 2291 1031
Size: 1260 2852
NspI r_CATG'y
Cuts at: 1009 1165 1665 2572 1009
Size: 156 500 907 2549
Fragments arranged by size:
2549 907 500 156
PasI CC'CwG_GG
Cuts at: 699 699
Size: 4112
PciI A'CATG_T
Cuts at: 1005 2568 1005
Size: 1563 2549
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PmlI CAC'GTG
Cuts at: 650 650
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 4080
PpuMI rG'GwC_Cy
Cuts at: 270 737 823 270
Size: 467 86 3559
Fragments arranged by size:
3559 467 86
PstI C_TGCA'G
Cuts at: 851 1040 1159 1844 851
Size: 189 119 685 3119
Fragments arranged by size:
3119 685 189 119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 818 1537 1897 818
Size: 719 360 3033
Fragments arranged by size:
3033 719 360
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 1126 1126
Size: 4112
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SanDI GG'GwC_CC
Cuts at: 823 823
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 3629 3629
Size: 4112
SexAI A'CCwGG_T
Cuts at: 338 338
Size: 4112
SfcI C'TryA_G
Cuts at: 33 447 847 1036 1155 1840 2833 3024 33
Size: 414 400 189 119 685 993 191 1121
Fragments arranged by size:
1121 993 685 414 400 191 189 119
SfoI GGC'GCC
Cuts at: 766 1095 1544 1792 766
Size: 329 449 248 3086
Fragments arranged by size:
3086 449 329 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 1134 1134
Size: 4112
SmlI C'TyrA_G
Cuts at: 1267 2182 2674 2936 1267
Size: 915 492 262 2443
Fragments arranged by size:
2443 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 1165 1665 1165
Size: 500 3612
StuI AGG'CCT
Cuts at: 184 184
Size: 4112
StyI C'CwwG_G
Cuts at: 107 557 1246 1600 107
Size: 450 689 354 2619
Fragments arranged by size:
2619 689 450 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 381 1059 1695 1828 2287 2496 381
Size: 678 636 133 459 209 1997
Fragments arranged by size:
1997 678 636 459 209 133
TatI w'GTAC_w
Cuts at: 3627 3627
Size: 4112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3629 3661 3629
Size: 32 4080
Tth111I GACn'n_nGTC
Cuts at: 1909 1909
Size: 4112
XbaI T'CTAG_A
Cuts at: 1143 1143
Size: 4112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1607 1607
Size: 4112
XmaI C'CCGG_G
Cuts at: 1132 1132
Size: 4112
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PshAI PsiI PspOMI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI