Restriction map for
pF1KB8797
(Circular) (Six-base) MAPSORT of: pF1KB8797.seq Check: 1655 from: 1 to: 4112
pF1KB8797 4112 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 365 1086 365
Size: 721 3391
AccI GT'mk_AC
Cuts at: 1150 1150
Size: 4112
Acc65I G'GTAC_C
Cuts at: 1128 1128
Size: 4112
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1937 2369 3116 3384 1937
Size: 432 747 268 2665
Fragments arranged by size:
2665 747 432 268
AflIII A'CryG_T
Cuts at: 296 545 1464 2568 3545 296
Size: 249 919 1104 977 863
Fragments arranged by size:
1104 977 919 863 249
AgeI A'CCGG_T
Cuts at: 2500 2500
Size: 4112
AleI CACnn'nnGTG
Cuts at: 679 679
Size: 4112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 304 304
Size: 4112
AlwNI CAG_nnn'CTG
Cuts at: 171 664 1101 1417 2984 171
Size: 493 437 316 1567 1299
Fragments arranged by size:
1567 1299 493 437 316
ApaLI G'TGCA_C
Cuts at: 352 500 825 2882 352
Size: 148 325 2057 1582
Fragments arranged by size:
2057 1582 325 148
ApoI r'AATT_y
Cuts at: 1116 3465 3721 1116
Size: 2349 256 1507
Fragments arranged by size:
2349 1507 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 369 1132 369
Size: 763 3349
BamHI G'GATC_C
Cuts at: 1137 1137
Size: 4112
BanI G'GyrC_C
Cuts at: 699 1128 1542 1790 1825 699
Size: 429 414 248 35 2986
Fragments arranged by size:
2986 429 414 248 35
BanII G_rGCy'C
Cuts at: 376 794 842 1126 2156 376
Size: 418 48 284 1030 2332
Fragments arranged by size:
2332 1030 418 284 48
BbeI G_GCGC'C
Cuts at: 1546 1794 1546
Size: 248 3864
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 1632 1632
Size: 4112
BglII A'GATC_T
Cuts at: 1627 3664 1627
Size: 2037 2075
BlpI GC'TnA_GC
Cuts at: 334 475 1224 334
Size: 141 749 3222
Fragments arranged by size:
3222 749 141
Bme1580I G_kGCm'C
Cuts at: 356 504 829 875 1737 1830 2886 356
Size: 148 325 46 862 93 1056 1582
Fragments arranged by size:
1582 1056 862 325 148 93 46
BmgBI CAC'GTC
Cuts at: 297 297
Size: 4112
BmrI ACTGGGnnnn_n'
Cuts at: 1324 1405 1740 3884 1324
Size: 81 335 2144 1552
Fragments arranged by size:
2144 1552 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 951 808
Size: 143 3969
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 2167 2659 2957 1288
Size: 879 492 298 2443
Fragments arranged by size:
2443 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3816 3816
Size: 4112
BsaAI yAC'GTr
Cuts at: 414 546 696 1465 414
Size: 132 150 769 3061
Fragments arranged by size:
3061 769 150 132
BsaBI GATnn'nnATC
Cuts at: 6 1626 3663 6
Size: 1620 2037 455
Fragments arranged by size:
2037 1620 455
BsaHI Gr'CG_yC
Cuts at: 671 847 1543 1791 2493 3989 671
Size: 176 696 248 702 1496 794
Fragments arranged by size:
1496 794 702 696 248 176
BsaWI w'CCGG_w
Cuts at: 1301 1822 2500 2774 2921 3332 1301
Size: 521 678 274 147 411 2081
Fragments arranged by size:
2081 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 579 163
Size: 416 3696
BseYI C'CCAG_C
Cuts at: 177 201 867 1018 1537 2295 2872 3368 177
Size: 24 666 151 519 758 577 496 921
Fragments arranged by size:
921 758 666 577 519 496 151 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 1111 913
Size: 198 3914
BsiEI CG_ry'CG
Cuts at: 104 1654 1700 2908 104
Size: 1550 46 1208 1308
Fragments arranged by size:
1550 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 335 356 376 504 829 1126 1904 2094 2886
Size: 21 20 128 325 297 778 190 792
Cuts at: 2886 335
Size: 1561
Fragments arranged by size:
1561 792 778 325 297 190 128 21
20
Bsp1286I G_dGCh'C
Cuts at: 335 356 376 504 794 829 842 875 1126
Size: 21 20 128 290 35 13 33 251
Cuts at: 1126 1737 1830 1904 2094 2156 2886 335
Size: 611 93 74 190 62 730 1561
Fragments arranged by size:
1561 730 611 290 251 190 128 93
74 62 35 33 21 20 13
BspEI T'CCGG_A
Cuts at: 3332 3332
Size: 4112
BspHI T'CATG_A
Cuts at: 3288 3288
Size: 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 179 365 1086 1162 1376 1678 2059 179
Size: 186 721 76 214 302 381 2232
Fragments arranged by size:
2232 721 381 302 214 186 76
BsrBI CCG'CTC
Cuts at: 50 2404 2458 3942 4107 50
Size: 2354 54 1484 165 55
Fragments arranged by size:
2354 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2024 2024
Size: 4112
BsrFI r'CCGG_y
Cuts at: 782 1061 2110 2291 2500 782
Size: 279 1049 181 209 2394
Fragments arranged by size:
2394 1049 279 209 181
BsrGI T'GTAC_A
Cuts at: 530 530
Size: 4112
BssHII G'CGCG_C
Cuts at: 569 629 569
Size: 60 4052
BssSI C'ACGA_G
Cuts at: 2383 2741 2383
Size: 358 3754
BstAPI GCAn_nnn'nTGC
Cuts at: 664 1098 1489 664
Size: 434 391 3287
Fragments arranged by size:
3287 434 391
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 957 957
Size: 4112
BstYI r'GATC_y
Cuts at: 1 425 1137 1604 1627 1962 2208 3220 3306
Size: 424 712 467 23 335 246 1012 86
Cuts at: 3306 3318 3664 1
Size: 12 346 449
Fragments arranged by size:
1012 712 467 449 424 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 508 902 508
Size: 394 3718
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2062 2243 2406 2062
Size: 181 163 3768
Fragments arranged by size:
3768 181 163
BtsI GCAGTG_nn'
Cuts at: 323 418 502 1065 1355 323
Size: 95 84 563 290 3080
Fragments arranged by size:
3080 563 290 95 84
DraI TTT'AAA
Cuts at: 1111 1111
Size: 4112
DraIII CAC_nnn'GTG
Cuts at: 824 936 824
Size: 112 4000
DrdI GACnn_nn'nnGTC
Cuts at: 1818 2676 1818
Size: 858 3254
EaeI y'GGCC_r
Cuts at: 257 452 521 710 815 1697 1871 2262 2289
Size: 195 69 189 105 882 174 391 27
Cuts at: 2289 3634 257
Size: 1345 735
Fragments arranged by size:
1345 882 735 391 195 189 174 105
69 27
EagI C'GGCC_G
Cuts at: 1697 1697
Size: 4112
EarI CTCTTCn'nnn_
Cuts at: 604 2135 2345 604
Size: 1531 210 2371
Fragments arranged by size:
2371 1531 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2320 2630 2776 3922 2320
Size: 310 146 1146 2510
Fragments arranged by size:
2510 1146 310 146
EcoICRI GAG'CTC
Cuts at: 374 1124 374
Size: 750 3362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 951 1937 2369 3116 3384 808
Size: 143 986 432 747 268 1536
Fragments arranged by size:
1536 986 747 432 268 143
EcoO109I rG'GnC_Cy
Cuts at: 168 1251 168
Size: 1083 3029
EcoRI G'AATT_C
Cuts at: 1116 1116
Size: 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 4112
FspI TGC'GCA
Cuts at: 1893 1893
Size: 4112
HaeII r_GCGC'y
Cuts at: 341 388 1546 1794 2816 3795 341
Size: 47 1158 248 1022 979 658
Fragments arranged by size:
1158 1022 979 658 248 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1954 1984 1986 2016 1954
Size: 30 2 30 4050
Fragments arranged by size:
4050 30 30 2
HincII GTy'rAC
Cuts at: 309 1151 309
Size: 842 3270
KasI G'GCGC_C
Cuts at: 1542 1790 1542
Size: 248 3864
KpnI G_GTAC'C
Cuts at: 1132 1132
Size: 4112
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1053 2783 2967 1053
Size: 1730 184 2198
Fragments arranged by size:
2198 1730 184
MscI TGG'CCA
Cuts at: 259 454 523 712 817 1873 259
Size: 195 69 189 105 1056 2498
Fragments arranged by size:
2498 1056 195 189 105 69
MslI CAynn'nnrTG
Cuts at: 286 316 679 1087 2101 2228 286
Size: 30 363 408 1014 127 2170
Fragments arranged by size:
2170 1014 408 363 127 30
MspA1I CmG'CkG
Cuts at: 122 177 510 1223 1537 1897 2910 3155 122
Size: 55 333 713 314 360 1013 245 1079
Fragments arranged by size:
1079 1013 713 360 333 314 245 55
NaeI GCC'GGC
Cuts at: 784 2293 784
Size: 1509 2603
NarI GG'CG_CC
Cuts at: 1543 1791 1543
Size: 248 3864
NgoMIV G'CCGG_C
Cuts at: 782 2291 782
Size: 1509 2603
NruI TCG'CGA
Cuts at: 891 891
Size: 4112
NspI r_CATG'y
Cuts at: 1165 1665 2572 1165
Size: 500 907 2705
Fragments arranged by size:
2705 907 500
PciI A'CATG_T
Cuts at: 2568 2568
Size: 4112
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PmlI CAC'GTG
Cuts at: 414 546 414
Size: 132 3980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 4080
PpuMI rG'GwC_Cy
Cuts at: 168 168
Size: 4112
PstI C_TGCA'G
Cuts at: 121 176 425 613 1159 1844 121
Size: 55 249 188 546 685 2389
Fragments arranged by size:
2389 685 546 249 188 55
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 122 177 1537 1897 122
Size: 55 1360 360 2337
Fragments arranged by size:
2337 1360 360 55
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 376 1126 376
Size: 750 3362
SacII CC_GC'GG
Cuts at: 511 511
Size: 4112
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 604 2135 2345 604
Size: 1531 210 2371
Fragments arranged by size:
2371 1531 210
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 3629 3629
Size: 4112
SfcI C'TryA_G
Cuts at: 33 117 172 421 609 1155 1840 2833 3024
Size: 84 55 249 188 546 685 993 191
Cuts at: 3024 33
Size: 1121
Fragments arranged by size:
1121 993 685 546 249 191 188 84
55
SfoI GGC'GCC
Cuts at: 1544 1792 1544
Size: 248 3864
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 1134 1134
Size: 4112
SmlI C'TyrA_G
Cuts at: 1267 2182 2674 2936 1267
Size: 915 492 262 2443
Fragments arranged by size:
2443 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 1165 1665 1165
Size: 500 3612
StuI AGG'CCT
Cuts at: 326 326
Size: 4112
StyI C'CwwG_G
Cuts at: 1246 1600 1246
Size: 354 3758
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1695 1828 2287 2496 1695
Size: 133 459 209 3311
Fragments arranged by size:
3311 459 209 133
TatI w'GTAC_w
Cuts at: 299 458 530 3627 299
Size: 159 72 3097 784
Fragments arranged by size:
3097 784 159 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 410 442 3629 3661 410
Size: 32 3187 32 861
Fragments arranged by size:
3187 861 32 32
Tth111I GACn'n_nGTC
Cuts at: 1909 1909
Size: 4112
XbaI T'CTAG_A
Cuts at: 1143 1143
Size: 4112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1607 1607
Size: 4112
XmaI C'CCGG_G
Cuts at: 1132 1132
Size: 4112
XmnI GAAnn'nnTTC
Cuts at: 943 943
Size: 4112
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NruI NspI PciI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BstEII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NsiI PacI PasI PflMI
PfoI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI