Restriction map for
pF1KB8800
(Circular) (Six-base) MAPSORT of: pF1KB8800.seq Check: 4008 from: 1 to: 4139
pF1KB8800 4139 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 478 478
Size: 4139
AccI GT'mk_AC
Cuts at: 1177 1177
Size: 4139
Acc65I G'GTAC_C
Cuts at: 1155 1155
Size: 4139
AclI AA'CG_TT
Cuts at: 3979 3979
Size: 4139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1964 2396 3143 3411 1006
Size: 958 432 747 268 1734
Fragments arranged by size:
1734 958 747 432 268
AflIII A'CryG_T
Cuts at: 449 1491 2595 3572 449
Size: 1042 1104 977 1016
Fragments arranged by size:
1104 1042 1016 977
AgeI A'CCGG_T
Cuts at: 2527 2527
Size: 4139
AlwNI CAG_nnn'CTG
Cuts at: 300 1444 3011 300
Size: 1144 1567 1428
Fragments arranged by size:
1567 1428 1144
ApaLI G'TGCA_C
Cuts at: 2909 2909
Size: 4139
ApoI r'AATT_y
Cuts at: 1143 3492 3748 1143
Size: 2349 256 1534
Fragments arranged by size:
2349 1534 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4139
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
AvaI C'yCGr_G
Cuts at: 1159 1159
Size: 4139
BamHI G'GATC_C
Cuts at: 1164 1164
Size: 4139
BanI G'GyrC_C
Cuts at: 1155 1569 1817 1852 1155
Size: 414 248 35 3442
Fragments arranged by size:
3442 414 248 35
BanII G_rGCy'C
Cuts at: 1153 2183 1153
Size: 1030 3109
BbeI G_GCGC'C
Cuts at: 1573 1821 1573
Size: 248 3891
BbsI GAAGACnn'nnnn_
Cuts at: 137 137
Size: 4139
BciVI GTATCCnnnnn_n'
Cuts at: 2037 2798 4139 2037
Size: 761 1341 2037
Fragments arranged by size:
2037 1341 761
BclI T'GATC_A
Cuts at: 1659 1659
Size: 4139
BglI GCCn_nnn'nGGC
Cuts at: 425 514 425
Size: 89 4050
BglII A'GATC_T
Cuts at: 790 1654 3691 790
Size: 864 2037 1238
Fragments arranged by size:
2037 1238 864
BlpI GC'TnA_GC
Cuts at: 1251 1251
Size: 4139
Bme1580I G_kGCm'C
Cuts at: 1764 1857 2913 1764
Size: 93 1056 2990
Fragments arranged by size:
2990 1056 93
BmgBI CAC'GTC
Cuts at: 452 452
Size: 4139
BmrI ACTGGGnnnn_n'
Cuts at: 433 709 888 1351 1432 1767 3911 433
Size: 276 179 463 81 335 2144 661
Fragments arranged by size:
2144 661 463 335 276 179 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 791
Size: 4139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1315 2194 2686 2984 1315
Size: 879 492 298 2470
Fragments arranged by size:
2470 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1053 3843 1053
Size: 2790 1349
BsaAI yAC'GTr
Cuts at: 1492 1492
Size: 4139
BsaBI GATnn'nnATC
Cuts at: 6 1653 3690 6
Size: 1647 2037 455
Fragments arranged by size:
2037 1647 455
BsaHI Gr'CG_yC
Cuts at: 1570 1818 2520 4016 1570
Size: 248 702 1496 1693
Fragments arranged by size:
1693 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1328 1849 2527 2801 2948 3359 1328
Size: 521 678 274 147 411 2108
Fragments arranged by size:
2108 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 184
Size: 4139
BseYI C'CCAG_C
Cuts at: 1564 2322 2899 3395 1564
Size: 758 577 496 2308
Fragments arranged by size:
2308 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 336 336
Size: 4139
BsiEI CG_ry'CG
Cuts at: 104 1681 1727 2935 104
Size: 1577 46 1208 1308
Fragments arranged by size:
1577 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 968 1153 1931 2121 2913 968
Size: 185 778 190 792 2194
Fragments arranged by size:
2194 792 778 190 185
Bsp1286I G_dGCh'C
Cuts at: 968 1153 1764 1857 1931 2121 2183 2913 968
Size: 185 611 93 74 190 62 730 2194
Fragments arranged by size:
2194 730 611 190 185 93 74 62
BspEI T'CCGG_A
Cuts at: 3359 3359
Size: 4139
BspHI T'CATG_A
Cuts at: 854 3315 854
Size: 2461 1678
BspMI ACCTGCnnnn'nnnn_
Cuts at: 478 1189 1403 1705 2086 478
Size: 711 214 302 381 2531
Fragments arranged by size:
2531 711 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2431 2485 3969 4134 50
Size: 2381 54 1484 165 55
Fragments arranged by size:
2381 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 624 2051 624
Size: 1427 2712
BsrFI r'CCGG_y
Cuts at: 2137 2318 2527 2137
Size: 181 209 3749
Fragments arranged by size:
3749 209 181
BssSI C'ACGA_G
Cuts at: 2410 2768 2410
Size: 358 3781
BstAPI GCAn_nnn'nTGC
Cuts at: 1516 1516
Size: 4139
BstBI TT'CG_AA
Cuts at: 993 2500 993
Size: 1507 2632
BstEII G'GTnAC_C
Cuts at: 555 649 555
Size: 94 4045
BstYI r'GATC_y
Cuts at: 1 790 1164 1631 1654 1989 2235 3247 3333
Size: 789 374 467 23 335 246 1012 86
Cuts at: 3333 3345 3691 1
Size: 12 346 449
Fragments arranged by size:
1012 789 467 449 374 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 943 943
Size: 4139
BtgI C'CryG_G
Cuts at: 107 107
Size: 4139
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 918 2089 2270 2433 918
Size: 1171 181 163 2624
Fragments arranged by size:
2624 1171 181 163
BtsI GCAGTG_nn'
Cuts at: 715 757 1382 715
Size: 42 625 3472
Fragments arranged by size:
3472 625 42
DraI TTT'AAA
Cuts at: 1138 1138
Size: 4139
DraIII CAC_nnn'GTG
Cuts at: 1107 1107
Size: 4139
DrdI GACnn_nn'nnGTC
Cuts at: 1845 2703 1845
Size: 858 3281
EaeI y'GGCC_r
Cuts at: 399 1724 1898 2289 2316 3661 399
Size: 1325 174 391 27 1345 877
Fragments arranged by size:
1345 1325 877 391 174 27
EagI C'GGCC_G
Cuts at: 1724 1724
Size: 4139
EarI CTCTTCn'nnn_
Cuts at: 2162 2372 2162
Size: 210 3929
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2347 2657 2803 3949 2347
Size: 310 146 1146 2537
Fragments arranged by size:
2537 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1151 1151
Size: 4139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1006 1964 2396 3143 3411 791
Size: 215 958 432 747 268 1519
Fragments arranged by size:
1519 958 747 432 268 215
EcoO109I rG'GnC_Cy
Cuts at: 947 1278 947
Size: 331 3808
EcoRI G'AATT_C
Cuts at: 1143 1143
Size: 4139
EcoRV GAT'ATC
Cuts at: 581 581
Size: 4139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 822 2158 822
Size: 1336 2803
FspI TGC'GCA
Cuts at: 1920 1920
Size: 4139
HaeII r_GCGC'y
Cuts at: 1573 1821 2843 3822 1573
Size: 248 1022 979 1890
Fragments arranged by size:
1890 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1981 2011 2013 2043 1981
Size: 30 2 30 4077
Fragments arranged by size:
4077 30 30 2
HincII GTy'rAC
Cuts at: 1178 1178
Size: 4139
KasI G'GCGC_C
Cuts at: 1569 1817 1569
Size: 248 3891
KpnI G_GTAC'C
Cuts at: 1159 1159
Size: 4139
MluI A'CGCG_T
Cuts at: 3572 3572
Size: 4139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2810 2994 2810
Size: 184 3955
MscI TGG'CCA
Cuts at: 401 1900 401
Size: 1499 2640
MslI CAynn'nnrTG
Cuts at: 454 478 496 574 691 916 919 1056 1120
Size: 24 18 78 117 225 3 137 64
Cuts at: 1120 2128 2255 454
Size: 1008 127 2338
Fragments arranged by size:
2338 1008 225 137 127 117 78 64
24 18 3
MspA1I CmG'CkG
Cuts at: 1250 1564 1924 2937 3182 1250
Size: 314 360 1013 245 2207
Fragments arranged by size:
2207 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2320 2320
Size: 4139
NarI GG'CG_CC
Cuts at: 1570 1818 1570
Size: 248 3891
NcoI C'CATG_G
Cuts at: 107 107
Size: 4139
NdeI CA'TA_TG
Cuts at: 643 643
Size: 4139
NgoMIV G'CCGG_C
Cuts at: 2318 2318
Size: 4139
NspI r_CATG'y
Cuts at: 219 1125 1192 1692 2599 219
Size: 906 67 500 907 1759
Fragments arranged by size:
1759 907 906 500 67
PciI A'CATG_T
Cuts at: 2595 2595
Size: 4139
PflMI CCAn_nnn'nTGG
Cuts at: 1107 1107
Size: 4139
PmeI GTTT'AAAC
Cuts at: 1138 1138
Size: 4139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 4107
PstI C_TGCA'G
Cuts at: 191 1186 1871 191
Size: 995 685 2459
Fragments arranged by size:
2459 995 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4139
PvuII CAG'CTG
Cuts at: 1564 1924 1564
Size: 360 3779
RsrII CG'GwC_CG
Cuts at: 2334 2334
Size: 4139
SacI G_AGCT'C
Cuts at: 1153 1153
Size: 4139
SalI G'TCGA_C
Cuts at: 1176 1176
Size: 4139
SapI GCTCTTCn'nnn_
Cuts at: 2162 2372 2162
Size: 210 3929
SbfI CC_TGCA'GG
Cuts at: 1186 1186
Size: 4139
ScaI AGT'ACT
Cuts at: 3656 3656
Size: 4139
SexAI A'CCwGG_T
Cuts at: 221 338 221
Size: 117 4022
SfcI C'TryA_G
Cuts at: 33 187 1182 1867 2860 3051 33
Size: 154 995 685 993 191 1121
Fragments arranged by size:
1121 995 993 685 191 154
SfiI GGCCn_nnn'nGGCC
Cuts at: 514 514
Size: 4139
SfoI GGC'GCC
Cuts at: 1571 1819 1571
Size: 248 3891
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
SmaI CCC'GGG
Cuts at: 1161 1161
Size: 4139
SmlI C'TyrA_G
Cuts at: 1294 2209 2701 2963 1294
Size: 915 492 262 2470
Fragments arranged by size:
2470 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4139
SphI G_CATG'C
Cuts at: 1192 1692 1192
Size: 500 3639
StuI AGG'CCT
Cuts at: 517 517
Size: 4139
StyI C'CwwG_G
Cuts at: 107 512 1041 1273 1627 107
Size: 405 529 232 354 2619
Fragments arranged by size:
2619 529 405 354 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 381 564 703 1722 1855 2314 2523 381
Size: 183 139 1019 133 459 209 1997
Fragments arranged by size:
1997 1019 459 209 183 139 133
TatI w'GTAC_w
Cuts at: 3654 3654
Size: 4139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3656 3688 3656
Size: 32 4107
Tth111I GACn'n_nGTC
Cuts at: 1936 1936
Size: 4139
XbaI T'CTAG_A
Cuts at: 1170 1170
Size: 4139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 133 610 1634 133
Size: 477 1024 2638
Fragments arranged by size:
2638 1024 477
XmaI C'CCGG_G
Cuts at: 1159 1159
Size: 4139
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I
ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI