Restriction map for
pF1KB8806
(Circular) (Six-base) MAPSORT of: pF1KB8806.seq Check: 2191 from: 1 to: 4178
pF1KB8806 4178 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 661 661
Size: 4178
AccI GT'mk_AC
Cuts at: 1216 1216
Size: 4178
Acc65I G'GTAC_C
Cuts at: 500 1194 500
Size: 694 3484
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2003 2435 3182 3450 2003
Size: 432 747 268 2731
Fragments arranged by size:
2731 747 432 268
AflIII A'CryG_T
Cuts at: 1530 2634 3611 1530
Size: 1104 977 2097
Fragments arranged by size:
2097 1104 977
AgeI A'CCGG_T
Cuts at: 2566 2566
Size: 4178
AleI CACnn'nnGTG
Cuts at: 989 1061 989
Size: 72 4106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 192 192
Size: 4178
AlwNI CAG_nnn'CTG
Cuts at: 708 1483 3050 708
Size: 775 1567 1836
Fragments arranged by size:
1836 1567 775
ApaI G_GGCC'C
Cuts at: 210 585 210
Size: 375 3803
ApaLI G'TGCA_C
Cuts at: 1072 2948 1072
Size: 1876 2302
ApoI r'AATT_y
Cuts at: 637 1182 3531 3787 637
Size: 545 2349 256 1028
Fragments arranged by size:
2349 1028 545 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 1198 1198
Size: 4178
BamHI G'GATC_C
Cuts at: 1203 1203
Size: 4178
BanI G'GyrC_C
Cuts at: 500 1194 1608 1856 1891 500
Size: 694 414 248 35 2787
Fragments arranged by size:
2787 694 414 248 35
BanII G_rGCy'C
Cuts at: 210 585 1192 2222 210
Size: 375 607 1030 2166
Fragments arranged by size:
2166 1030 607 375
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BciVI GTATCCnnnnn_n'
Cuts at: 2076 2837 4178 2076
Size: 761 1341 2076
Fragments arranged by size:
2076 1341 761
BclI T'GATC_A
Cuts at: 752 842 1698 752
Size: 90 856 3232
Fragments arranged by size:
3232 856 90
BglI GCCn_nnn'nGGC
Cuts at: 205 535 595 205
Size: 330 60 3788
Fragments arranged by size:
3788 330 60
BglII A'GATC_T
Cuts at: 467 1693 3730 467
Size: 1226 2037 915
Fragments arranged by size:
2037 1226 915
BlpI GC'TnA_GC
Cuts at: 1290 1290
Size: 4178
Bme1580I G_kGCm'C
Cuts at: 210 540 585 1076 1803 1896 2952 210
Size: 330 45 491 727 93 1056 1436
Fragments arranged by size:
1436 1056 727 491 330 93 45
BmrI ACTGGGnnnn_n'
Cuts at: 411 994 1390 1471 1806 3950 411
Size: 583 396 81 335 2144 639
Fragments arranged by size:
2144 639 583 396 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 4178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 1135
Size: 4178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1354 2233 2725 3023 1354
Size: 879 492 298 2509
Fragments arranged by size:
2509 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3882 3882
Size: 4178
BsaAI yAC'GTr
Cuts at: 1531 1531
Size: 4178
BsaBI GATnn'nnATC
Cuts at: 6 1692 3729 6
Size: 1686 2037 455
Fragments arranged by size:
2037 1686 455
BsaHI Gr'CG_yC
Cuts at: 1609 1857 2559 4055 1609
Size: 248 702 1496 1732
Fragments arranged by size:
1732 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1031 1367 1888 2566 2840 2987 3398 1031
Size: 336 521 678 274 147 411 1811
Fragments arranged by size:
1811 678 521 411 336 274 147
BseYI C'CCAG_C
Cuts at: 1165 1603 2361 2938 3434 1165
Size: 438 758 577 496 1909
Fragments arranged by size:
1909 758 577 496 438
BsiEI CG_ry'CG
Cuts at: 104 1720 1766 2974 104
Size: 1616 46 1208 1308
Fragments arranged by size:
1616 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1076 1192 1970 2160 2952 1076
Size: 116 778 190 792 2302
Fragments arranged by size:
2302 792 778 190 116
Bsp1286I G_dGCh'C
Cuts at: 210 540 585 1076 1192 1803 1896 1970 2160
Size: 330 45 491 116 611 93 74 190
Cuts at: 2160 2222 2952 210
Size: 62 730 1436
Fragments arranged by size:
1436 730 611 491 330 190 116 93
74 62 45
BspEI T'CCGG_A
Cuts at: 1031 3398 1031
Size: 2367 1811
BspHI T'CATG_A
Cuts at: 749 839 3354 749
Size: 90 2515 1573
Fragments arranged by size:
2515 1573 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 356 661 1049 1228 1442 1744 2125 356
Size: 305 388 179 214 302 381 2409
Fragments arranged by size:
2409 388 381 305 302 214 179
BsrBI CCG'CTC
Cuts at: 50 2470 2524 4008 4173 50
Size: 2420 54 1484 165 55
Fragments arranged by size:
2420 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2090 2090
Size: 4178
BsrFI r'CCGG_y
Cuts at: 2176 2357 2566 2176
Size: 181 209 3788
Fragments arranged by size:
3788 209 181
BsrGI T'GTAC_A
Cuts at: 458 458
Size: 4178
BssSI C'ACGA_G
Cuts at: 2449 2807 2449
Size: 358 3820
BstAPI GCAn_nnn'nTGC
Cuts at: 1555 1555
Size: 4178
BstBI TT'CG_AA
Cuts at: 2539 2539
Size: 4178
BstYI r'GATC_y
Cuts at: 1 428 467 1203 1670 1693 2028 2274 3286
Size: 427 39 736 467 23 335 246 1012
Cuts at: 3286 3372 3384 3730 1
Size: 86 12 346 449
Fragments arranged by size:
1012 736 467 449 427 346 335 246
86 39 23 12
BtgI C'CryG_G
Cuts at: 107 1103 107
Size: 996 3182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2128 2309 2472 2128
Size: 181 163 3834
Fragments arranged by size:
3834 181 163
BtsI GCAGTG_nn'
Cuts at: 984 1421 984
Size: 437 3741
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 135 170 135
Size: 35 4143
DraI TTT'AAA
Cuts at: 1177 1177
Size: 4178
DraIII CAC_nnn'GTG
Cuts at: 522 522
Size: 4178
DrdI GACnn_nn'nnGTC
Cuts at: 1884 2742 1884
Size: 858 3320
EaeI y'GGCC_r
Cuts at: 335 506 587 1763 1937 2328 2355 3700 335
Size: 171 81 1176 174 391 27 1345 813
Fragments arranged by size:
1345 1176 813 391 174 171 81 27
EagI C'GGCC_G
Cuts at: 1763 1763
Size: 4178
EarI CTCTTCn'nnn_
Cuts at: 358 2201 2411 358
Size: 1843 210 2125
Fragments arranged by size:
2125 1843 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2386 2696 2842 3988 2386
Size: 310 146 1146 2576
Fragments arranged by size:
2576 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1190 1190
Size: 4178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 2003 2435 3182 3450 1135
Size: 868 432 747 268 1863
Fragments arranged by size:
1863 868 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 197 206 582 1317 197
Size: 9 376 735 3058
Fragments arranged by size:
3058 735 376 9
EcoRI G'AATT_C
Cuts at: 637 1182 637
Size: 545 3633
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 4178
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 1612 1860 2882 3861 1612
Size: 248 1022 979 1929
Fragments arranged by size:
1929 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 793 825 2020 2050 2052 2082 793
Size: 32 1195 30 2 30 2889
Fragments arranged by size:
2889 1195 32 30 30 2
HincII GTy'rAC
Cuts at: 996 1217 996
Size: 221 3957
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 504 1198 504
Size: 694 3484
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 237 2849 3033 237
Size: 2612 184 1382
Fragments arranged by size:
2612 1382 184
MscI TGG'CCA
Cuts at: 337 508 589 1939 337
Size: 171 81 1350 2576
Fragments arranged by size:
2576 1350 171 81
MslI CAynn'nnrTG
Cuts at: 268 923 989 1061 2167 2294 268
Size: 655 66 72 1106 127 2152
Fragments arranged by size:
2152 1106 655 127 72 66
MspA1I CmG'CkG
Cuts at: 1289 1603 1963 2976 3221 1289
Size: 314 360 1013 245 2246
Fragments arranged by size:
2246 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2359 2359
Size: 4178
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NcoI C'CATG_G
Cuts at: 107 107
Size: 4178
NgoMIV G'CCGG_C
Cuts at: 2357 2357
Size: 4178
NspI r_CATG'y
Cuts at: 1231 1731 2638 1231
Size: 500 907 2771
Fragments arranged by size:
2771 907 500
PciI A'CATG_T
Cuts at: 2634 2634
Size: 4178
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PsiI TTA'TAA
Cuts at: 775 775
Size: 4178
PspOMI G'GGCC_C
Cuts at: 206 581 206
Size: 375 3803
PstI C_TGCA'G
Cuts at: 658 1225 1910 658
Size: 567 685 2926
Fragments arranged by size:
2926 685 567
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 1603 1963 1603
Size: 360 3818
RsrII CG'GwC_CG
Cuts at: 542 2373 542
Size: 1831 2347
SacI G_AGCT'C
Cuts at: 1192 1192
Size: 4178
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 358 2201 2411 358
Size: 1843 210 2125
Fragments arranged by size:
2125 1843 210
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 3695 3695
Size: 4178
SexAI A'CCwGG_T
Cuts at: 1076 1076
Size: 4178
SfcI C'TryA_G
Cuts at: 33 654 1221 1906 2899 3090 33
Size: 621 567 685 993 191 1121
Fragments arranged by size:
1121 993 685 621 567 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 205 205
Size: 4178
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 1200 1200
Size: 4178
SmlI C'TyrA_G
Cuts at: 1333 2248 2740 3002 1333
Size: 915 492 262 2509
Fragments arranged by size:
2509 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 1231 1731 1231
Size: 500 3678
StyI C'CwwG_G
Cuts at: 107 233 1312 1666 107
Size: 126 1079 354 2619
Fragments arranged by size:
2619 1079 354 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 931 1761 1894 2353 2562 931
Size: 830 133 459 209 2547
Fragments arranged by size:
2547 830 459 209 133
TatI w'GTAC_w
Cuts at: 227 458 3693 227
Size: 231 3235 712
Fragments arranged by size:
3235 712 231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4146
Tth111I GACn'n_nGTC
Cuts at: 1975 1975
Size: 4178
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1673 1673
Size: 4178
XmaI C'CCGG_G
Cuts at: 1198 1198
Size: 4178
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI
BseRI BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspXI PsrI
SacII SanDI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI