Restriction map for
pF1KB8809
(Circular) (Six-base) MAPSORT of: pF1KB8809.seq Check: 1633 from: 1 to: 4193
pF1KB8809 4193 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1231 1231
Size: 4193
Acc65I G'GTAC_C
Cuts at: 1209 1209
Size: 4193
AclI AA'CG_TT
Cuts at: 4033 4033
Size: 4193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 2018 2450 3197 3465 910
Size: 1108 432 747 268 1638
Fragments arranged by size:
1638 1108 747 432 268
AflIII A'CryG_T
Cuts at: 1545 2649 3626 1545
Size: 1104 977 2112
Fragments arranged by size:
2112 1104 977
AgeI A'CCGG_T
Cuts at: 2581 2581
Size: 4193
AlwNI CAG_nnn'CTG
Cuts at: 601 1498 3065 601
Size: 897 1567 1729
Fragments arranged by size:
1729 1567 897
ApaLI G'TGCA_C
Cuts at: 526 2963 526
Size: 2437 1756
ApoI r'AATT_y
Cuts at: 276 1036 1115 1197 3546 3802 276
Size: 760 79 82 2349 256 667
Fragments arranged by size:
2349 760 667 256 82 79
AseI AT'TA_AT
Cuts at: 20 20
Size: 4193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
AvaI C'yCGr_G
Cuts at: 1213 1213
Size: 4193
BamHI G'GATC_C
Cuts at: 1218 1218
Size: 4193
BanI G'GyrC_C
Cuts at: 1209 1623 1871 1906 1209
Size: 414 248 35 3496
Fragments arranged by size:
3496 414 248 35
BanII G_rGCy'C
Cuts at: 227 1109 1207 2237 227
Size: 882 98 1030 2183
Fragments arranged by size:
2183 1030 882 98
BbeI G_GCGC'C
Cuts at: 1627 1875 1627
Size: 248 3945
BciVI GTATCCnnnnn_n'
Cuts at: 190 2091 2852 4193 190
Size: 1901 761 1341 190
Fragments arranged by size:
1901 1341 761 190
BclI T'GATC_A
Cuts at: 779 1713 779
Size: 934 3259
BglI GCCn_nnn'nGGC
Cuts at: 967 967
Size: 4193
BglII A'GATC_T
Cuts at: 1708 3745 1708
Size: 2037 2156
BlpI GC'TnA_GC
Cuts at: 1305 1305
Size: 4193
Bme1580I G_kGCm'C
Cuts at: 530 1818 1911 2967 530
Size: 1288 93 1056 1756
Fragments arranged by size:
1756 1288 1056 93
BmgBI CAC'GTC
Cuts at: 698 698
Size: 4193
BmrI ACTGGGnnnn_n'
Cuts at: 1405 1486 1821 3965 1405
Size: 81 335 2144 1633
Fragments arranged by size:
2144 1633 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4193
Bpu10I CC'TnA_GC
Cuts at: 316 316
Size: 4193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 564 1369 2248 2740 3038 564
Size: 805 879 492 298 1719
Fragments arranged by size:
1719 879 805 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 895 3897 895
Size: 3002 1191
BsaAI yAC'GTr
Cuts at: 1546 1546
Size: 4193
BsaBI GATnn'nnATC
Cuts at: 6 1169 1707 3744 6
Size: 1163 538 2037 455
Fragments arranged by size:
2037 1163 538 455
BsaHI Gr'CG_yC
Cuts at: 1624 1872 2574 4070 1624
Size: 248 702 1496 1747
Fragments arranged by size:
1747 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1382 1903 2581 2855 3002 3413 1382
Size: 521 678 274 147 411 2162
Fragments arranged by size:
2162 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1099 1099
Size: 4193
BseYI C'CCAG_C
Cuts at: 1135 1618 2376 2953 3449 1135
Size: 483 758 577 496 1879
Fragments arranged by size:
1879 758 577 496 483
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 918 918
Size: 4193
BsiEI CG_ry'CG
Cuts at: 104 1735 1781 2989 104
Size: 1631 46 1208 1308
Fragments arranged by size:
1631 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 227 530 893 1091 1207 1985 2175 2967 227
Size: 303 363 198 116 778 190 792 1453
Fragments arranged by size:
1453 792 778 363 303 198 190 116
Bsp1286I G_dGCh'C
Cuts at: 227 530 893 1091 1109 1207 1818 1911 1985
Size: 303 363 198 18 98 611 93 74
Cuts at: 1985 2175 2237 2967 227
Size: 190 62 730 1453
Fragments arranged by size:
1453 730 611 363 303 198 190 98
93 74 62 18
BspEI T'CCGG_A
Cuts at: 3413 3413
Size: 4193
BspHI T'CATG_A
Cuts at: 497 1167 3369 497
Size: 670 2202 1321
Fragments arranged by size:
2202 1321 670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 347 1078 1243 1457 1759 2140 347
Size: 731 165 214 302 381 2400
Fragments arranged by size:
2400 731 381 302 214 165
BsrBI CCG'CTC
Cuts at: 50 2485 2539 4023 4188 50
Size: 2435 54 1484 165 55
Fragments arranged by size:
2435 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2105 2105
Size: 4193
BsrFI r'CCGG_y
Cuts at: 2191 2372 2581 2191
Size: 181 209 3803
Fragments arranged by size:
3803 209 181
BssSI C'ACGA_G
Cuts at: 2464 2822 2464
Size: 358 3835
BstAPI GCAn_nnn'nTGC
Cuts at: 1570 1570
Size: 4193
BstBI TT'CG_AA
Cuts at: 2554 2554
Size: 4193
BstXI CCAn_nnnn'nTGG
Cuts at: 1102 1102
Size: 4193
BstYI r'GATC_y
Cuts at: 1 360 1218 1685 1708 2043 2289 3301 3387
Size: 359 858 467 23 335 246 1012 86
Cuts at: 3387 3399 3745 1
Size: 12 346 449
Fragments arranged by size:
1012 858 467 449 359 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 750 750
Size: 4193
BtgI C'CryG_G
Cuts at: 116 845 1160 116
Size: 729 315 3149
Fragments arranged by size:
3149 729 315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2143 2324 2487 2143
Size: 181 163 3849
Fragments arranged by size:
3849 181 163
BtsI GCAGTG_nn'
Cuts at: 993 1436 993
Size: 443 3750
DraI TTT'AAA
Cuts at: 1192 1192
Size: 4193
DraIII CAC_nnn'GTG
Cuts at: 534 698 534
Size: 164 4029
DrdI GACnn_nn'nnGTC
Cuts at: 1899 2757 1899
Size: 858 3335
EaeI y'GGCC_r
Cuts at: 1778 1952 2343 2370 3715 1778
Size: 174 391 27 1345 2256
Fragments arranged by size:
2256 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1778 1778
Size: 4193
EarI CTCTTCn'nnn_
Cuts at: 232 373 862 898 1066 2216 2426 232
Size: 141 489 36 168 1150 210 1999
Fragments arranged by size:
1999 1150 489 210 168 141 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2401 2711 2857 4003 2401
Size: 310 146 1146 2591
Fragments arranged by size:
2591 1146 310 146
EcoICRI GAG'CTC
Cuts at: 225 1205 225
Size: 980 3213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 2018 2450 3197 3465 910
Size: 1108 432 747 268 1638
Fragments arranged by size:
1638 1108 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 554 788 1077 1332 554
Size: 234 289 255 3415
Fragments arranged by size:
3415 289 255 234
EcoRI G'AATT_C
Cuts at: 1197 1197
Size: 4193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 855 2212 855
Size: 1357 2836
FspI TGC'GCA
Cuts at: 1974 1974
Size: 4193
HaeII r_GCGC'y
Cuts at: 1627 1875 2897 3876 1627
Size: 248 1022 979 1944
Fragments arranged by size:
1944 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 728 760 2035 2065 2067 2097 728
Size: 32 1275 30 2 30 2824
Fragments arranged by size:
2824 1275 32 30 30 2
HincII GTy'rAC
Cuts at: 1232 1232
Size: 4193
KasI G'GCGC_C
Cuts at: 1623 1871 1623
Size: 248 3945
KpnI G_GTAC'C
Cuts at: 1213 1213
Size: 4193
MfeI C'AATT_G
Cuts at: 521 521
Size: 4193
MluI A'CGCG_T
Cuts at: 3626 3626
Size: 4193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2864 3048 2864
Size: 184 4009
MscI TGG'CCA
Cuts at: 1954 1954
Size: 4193
MslI CAynn'nnrTG
Cuts at: 496 2182 2309 496
Size: 1686 127 2380
Fragments arranged by size:
2380 1686 127
MspA1I CmG'CkG
Cuts at: 1304 1618 1978 2991 3236 1304
Size: 314 360 1013 245 2261
Fragments arranged by size:
2261 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2374 2374
Size: 4193
NarI GG'CG_CC
Cuts at: 1624 1872 1624
Size: 248 3945
NcoI C'CATG_G
Cuts at: 1160 1160
Size: 4193
NgoMIV G'CCGG_C
Cuts at: 2372 2372
Size: 4193
NspI r_CATG'y
Cuts at: 1246 1746 2653 1246
Size: 500 907 2786
Fragments arranged by size:
2786 907 500
PciI A'CATG_T
Cuts at: 2649 2649
Size: 4193
PflMI CCAn_nnn'nTGG
Cuts at: 698 698
Size: 4193
PfoI T'CCnGG_A
Cuts at: 448 448
Size: 4193
PmeI GTTT'AAAC
Cuts at: 1192 1192
Size: 4193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 4161
PpuMI rG'GwC_Cy
Cuts at: 554 788 554
Size: 234 3959
PstI C_TGCA'G
Cuts at: 1075 1240 1925 1075
Size: 165 685 3343
Fragments arranged by size:
3343 685 165
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4193
PvuII CAG'CTG
Cuts at: 1618 1978 1618
Size: 360 3833
RsrII CG'GwC_CG
Cuts at: 2388 2388
Size: 4193
SacI G_AGCT'C
Cuts at: 227 1207 227
Size: 980 3213
SalI G'TCGA_C
Cuts at: 1230 1230
Size: 4193
SapI GCTCTTCn'nnn_
Cuts at: 232 373 898 1066 2216 2426 232
Size: 141 525 168 1150 210 1999
Fragments arranged by size:
1999 1150 525 210 168 141
SbfI CC_TGCA'GG
Cuts at: 1075 1240 1075
Size: 165 4028
ScaI AGT'ACT
Cuts at: 679 3710 679
Size: 3031 1162
SfcI C'TryA_G
Cuts at: 33 1054 1071 1236 1921 2914 3105 33
Size: 1021 17 165 685 993 191 1121
Fragments arranged by size:
1121 1021 993 685 191 165 17
SfoI GGC'GCC
Cuts at: 1625 1873 1625
Size: 248 3945
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
SmaI CCC'GGG
Cuts at: 1215 1215
Size: 4193
SmlI C'TyrA_G
Cuts at: 543 1348 2263 2755 3017 543
Size: 805 915 492 262 1719
Fragments arranged by size:
1719 915 805 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4193
SphI G_CATG'C
Cuts at: 1246 1746 1246
Size: 500 3693
StyI C'CwwG_G
Cuts at: 248 861 1160 1327 1681 248
Size: 613 299 167 354 2760
Fragments arranged by size:
2760 613 354 299 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1776 1909 2368 2577 1776
Size: 133 459 209 3392
Fragments arranged by size:
3392 459 209 133
TatI w'GTAC_w
Cuts at: 455 677 1046 3708 455
Size: 222 369 2662 940
Fragments arranged by size:
2662 940 369 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 266 298 3710 3742 266
Size: 32 3412 32 717
Fragments arranged by size:
3412 717 32 32
Tth111I GACn'n_nGTC
Cuts at: 1990 1990
Size: 4193
XbaI T'CTAG_A
Cuts at: 1224 1224
Size: 4193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 418 978 1688 418
Size: 560 710 2923
Fragments arranged by size:
2923 710 560
XmaI C'CCGG_G
Cuts at: 1213 1213
Size: 4193
XmnI GAAnn'nnTTC
Cuts at: 923 923
Size: 4193
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmtI BpmI BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI