Restriction map for
pF1KB8811
(Circular) (Six-base) MAPSORT of: pF1KB8811.seq Check: 3094 from: 1 to: 4199
pF1KB8811 4199 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 269 1237 269
Size: 968 3231
Acc65I G'GTAC_C
Cuts at: 1215 1215
Size: 4199
AclI AA'CG_TT
Cuts at: 4039 4039
Size: 4199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1192 2024 2456 3203 3471 1192
Size: 832 432 747 268 1920
Fragments arranged by size:
1920 832 747 432 268
AflIII A'CryG_T
Cuts at: 119 1551 2655 3632 119
Size: 1432 1104 977 686
Fragments arranged by size:
1432 1104 977 686
AgeI A'CCGG_T
Cuts at: 2587 2587
Size: 4199
AlwNI CAG_nnn'CTG
Cuts at: 1504 3071 1504
Size: 1567 2632
ApaLI G'TGCA_C
Cuts at: 2969 2969
Size: 4199
ApoI r'AATT_y
Cuts at: 1203 3552 3808 1203
Size: 2349 256 1594
Fragments arranged by size:
2349 1594 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4199
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
AvaI C'yCGr_G
Cuts at: 367 1219 367
Size: 852 3347
BamHI G'GATC_C
Cuts at: 356 1224 356
Size: 868 3331
BanI G'GyrC_C
Cuts at: 547 558 982 1215 1629 1877 1912 547
Size: 11 424 233 414 248 35 2834
Fragments arranged by size:
2834 424 414 248 233 35 11
BanII G_rGCy'C
Cuts at: 523 581 1213 2243 523
Size: 58 632 1030 2479
Fragments arranged by size:
2479 1030 632 58
BbeI G_GCGC'C
Cuts at: 551 562 986 1633 1881 551
Size: 11 424 647 248 2869
Fragments arranged by size:
2869 647 424 248 11
BbvCI CC'TCA_GC
Cuts at: 283 787 283
Size: 504 3695
BciVI GTATCCnnnnn_n'
Cuts at: 2097 2858 4199 2097
Size: 761 1341 2097
Fragments arranged by size:
2097 1341 761
BclI T'GATC_A
Cuts at: 1719 1719
Size: 4199
BglI GCCn_nnn'nGGC
Cuts at: 556 556
Size: 4199
BglII A'GATC_T
Cuts at: 1714 3751 1714
Size: 2037 2162
BlpI GC'TnA_GC
Cuts at: 1311 1311
Size: 4199
Bme1580I G_kGCm'C
Cuts at: 1824 1917 2973 1824
Size: 93 1056 3050
Fragments arranged by size:
3050 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1411 1492 1827 3971 1411
Size: 81 335 2144 1639
Fragments arranged by size:
2144 1639 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 4199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1096
Size: 4199
Bpu10I CC'TnA_GC
Cuts at: 283 787 283
Size: 504 3695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1375 2254 2746 3044 1375
Size: 879 492 298 2530
Fragments arranged by size:
2530 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 581 1072 3903 581
Size: 491 2831 877
Fragments arranged by size:
2831 877 491
BsaAI yAC'GTr
Cuts at: 122 138 831 1552 122
Size: 16 693 721 2769
Fragments arranged by size:
2769 721 693 16
BsaBI GATnn'nnATC
Cuts at: 6 1713 3750 6
Size: 1707 2037 455
Fragments arranged by size:
2037 1707 455
BsaHI Gr'CG_yC
Cuts at: 266 548 559 983 1630 1878 2580 4076 266
Size: 282 11 424 647 248 702 1496 389
Fragments arranged by size:
1496 702 647 424 389 282 248 11
BsaWI w'CCGG_w
Cuts at: 1388 1909 2587 2861 3008 3419 1388
Size: 521 678 274 147 411 2168
Fragments arranged by size:
2168 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 904 904
Size: 4199
BseYI C'CCAG_C
Cuts at: 261 543 1130 1624 2382 2959 3455 261
Size: 282 587 494 758 577 496 1005
Fragments arranged by size:
1005 758 587 577 496 494 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 325 994 1186 294
Size: 31 669 192 3307
Fragments arranged by size:
3307 669 192 31
BsiEI CG_ry'CG
Cuts at: 104 1741 1787 2995 104
Size: 1637 46 1208 1308
Fragments arranged by size:
1637 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 523 581 836 1213 1991 2181 2973 523
Size: 58 255 377 778 190 792 1749
Fragments arranged by size:
1749 792 778 377 255 190 58
Bsp1286I G_dGCh'C
Cuts at: 523 581 836 1213 1824 1917 1991 2181 2243
Size: 58 255 377 611 93 74 190 62
Cuts at: 2243 2973 523
Size: 730 1749
Fragments arranged by size:
1749 730 611 377 255 190 93 74
62 58
BspEI T'CCGG_A
Cuts at: 3419 3419
Size: 4199
BspHI T'CATG_A
Cuts at: 3375 3375
Size: 4199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 305 1025 1249 1463 1765 2146 305
Size: 720 224 214 302 381 2358
Fragments arranged by size:
2358 720 381 302 224 214
BsrBI CCG'CTC
Cuts at: 50 2491 2545 4029 4194 50
Size: 2441 54 1484 165 55
Fragments arranged by size:
2441 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2111 2111
Size: 4199
BsrFI r'CCGG_y
Cuts at: 2197 2378 2587 2197
Size: 181 209 3809
Fragments arranged by size:
3809 209 181
BsrGI T'GTAC_A
Cuts at: 302 302
Size: 4199
BssSI C'ACGA_G
Cuts at: 2470 2828 2470
Size: 358 3841
BstAPI GCAn_nnn'nTGC
Cuts at: 1017 1576 1017
Size: 559 3640
BstBI TT'CG_AA
Cuts at: 2560 2560
Size: 4199
BstXI CCAn_nnnn'nTGG
Cuts at: 216 543 807 861 216
Size: 327 264 54 3554
Fragments arranged by size:
3554 327 264 54
BstYI r'GATC_y
Cuts at: 1 356 1224 1691 1714 2049 2295 3307 3393
Size: 355 868 467 23 335 246 1012 86
Cuts at: 3393 3405 3751 1
Size: 12 346 449
Fragments arranged by size:
1012 868 467 449 355 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 360 509 524 536 626 685 107
Size: 253 149 15 12 90 59 3621
Fragments arranged by size:
3621 253 149 90 59 15 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 273 405 780 1007 2149 2330 2493 273
Size: 132 375 227 1142 181 163 1979
Fragments arranged by size:
1979 1142 375 227 181 163 132
BtsI GCAGTG_nn'
Cuts at: 1442 1442
Size: 4199
DraI TTT'AAA
Cuts at: 1198 1198
Size: 4199
DrdI GACnn_nn'nnGTC
Cuts at: 1905 2763 1905
Size: 858 3341
EaeI y'GGCC_r
Cuts at: 512 998 1031 1055 1136 1784 1958 2349 2376
Size: 486 33 24 81 648 174 391 27
Cuts at: 2376 3721 512
Size: 1345 990
Fragments arranged by size:
1345 990 648 486 391 174 81 33
27 24
EagI C'GGCC_G
Cuts at: 1784 1784
Size: 4199
EarI CTCTTCn'nnn_
Cuts at: 586 685 2222 2432 586
Size: 99 1537 210 2353
Fragments arranged by size:
2353 1537 210 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 155 964 1133 2407 2717 2863 4009 155
Size: 809 169 1274 310 146 1146 345
Fragments arranged by size:
1274 1146 809 345 310 169 146
EcoICRI GAG'CTC
Cuts at: 521 579 1211 521
Size: 58 632 3509
Fragments arranged by size:
3509 632 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1192 2024 2456 3203 3471 1096
Size: 96 832 432 747 268 1824
Fragments arranged by size:
1824 832 747 432 268 96
EcoNI CCTnn'n_nnAGG
Cuts at: 1095 1095
Size: 4199
EcoO109I rG'GnC_Cy
Cuts at: 371 1005 1338 371
Size: 634 333 3232
Fragments arranged by size:
3232 634 333
EcoRI G'AATT_C
Cuts at: 1203 1203
Size: 4199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 4199
FspI TGC'GCA
Cuts at: 1980 1980
Size: 4199
HaeII r_GCGC'y
Cuts at: 551 562 694 756 854 986 1633 1881 2903
Size: 11 132 62 98 132 647 248 1022
Cuts at: 2903 3882 551
Size: 979 868
Fragments arranged by size:
1022 979 868 647 248 132 132 98
62 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2041 2071 2073 2103 2041
Size: 30 2 30 4137
Fragments arranged by size:
4137 30 30 2
HincII GTy'rAC
Cuts at: 975 1238 975
Size: 263 3936
KasI G'GCGC_C
Cuts at: 547 558 982 1629 1877 547
Size: 11 424 647 248 2869
Fragments arranged by size:
2869 647 424 248 11
KpnI G_GTAC'C
Cuts at: 1219 1219
Size: 4199
MluI A'CGCG_T
Cuts at: 3632 3632
Size: 4199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2870 3054 2870
Size: 184 4015
MscI TGG'CCA
Cuts at: 1000 1033 1057 1960 1000
Size: 33 24 903 3239
Fragments arranged by size:
3239 903 33 24
MslI CAynn'nnrTG
Cuts at: 193 442 2188 2315 193
Size: 249 1746 127 2077
Fragments arranged by size:
2077 1746 249 127
MspA1I CmG'CkG
Cuts at: 321 1130 1170 1310 1624 1984 2997 3242 321
Size: 809 40 140 314 360 1013 245 1278
Fragments arranged by size:
1278 1013 809 360 314 245 140 40
NaeI GCC'GGC
Cuts at: 2380 2380
Size: 4199
NarI GG'CG_CC
Cuts at: 548 559 983 1630 1878 548
Size: 11 424 647 248 2869
Fragments arranged by size:
2869 647 424 248 11
NcoI C'CATG_G
Cuts at: 107 626 107
Size: 519 3680
NgoMIV G'CCGG_C
Cuts at: 2378 2378
Size: 4199
NspI r_CATG'y
Cuts at: 1252 1752 2659 1252
Size: 500 907 2792
Fragments arranged by size:
2792 907 500
PasI CC'CwG_GG
Cuts at: 872 1152 872
Size: 280 3919
PciI A'CATG_T
Cuts at: 2655 2655
Size: 4199
PflMI CCAn_nnn'nTGG
Cuts at: 1053 1053
Size: 4199
PmeI GTTT'AAAC
Cuts at: 1198 1198
Size: 4199
PmlI CAC'GTG
Cuts at: 122 138 831 122
Size: 16 693 3490
Fragments arranged by size:
3490 693 16
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 3338
Size: 32 4167
PpuMI rG'GwC_Cy
Cuts at: 371 371
Size: 4199
PstI C_TGCA'G
Cuts at: 1246 1931 1246
Size: 685 3514
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4199
PvuII CAG'CTG
Cuts at: 1130 1170 1624 1984 1130
Size: 40 454 360 3345
Fragments arranged by size:
3345 454 360 40
RsrII CG'GwC_CG
Cuts at: 2394 2394
Size: 4199
SacI G_AGCT'C
Cuts at: 523 581 1213 523
Size: 58 632 3509
Fragments arranged by size:
3509 632 58
SalI G'TCGA_C
Cuts at: 1236 1236
Size: 4199
SapI GCTCTTCn'nnn_
Cuts at: 586 2222 2432 586
Size: 1636 210 2353
Fragments arranged by size:
2353 1636 210
SbfI CC_TGCA'GG
Cuts at: 1246 1246
Size: 4199
ScaI AGT'ACT
Cuts at: 3716 3716
Size: 4199
SexAI A'CCwGG_T
Cuts at: 1160 1160
Size: 4199
SfcI C'TryA_G
Cuts at: 33 1242 1927 2920 3111 33
Size: 1209 685 993 191 1121
Fragments arranged by size:
1209 1121 993 685 191
SfoI GGC'GCC
Cuts at: 549 560 984 1631 1879 549
Size: 11 424 647 248 2869
Fragments arranged by size:
2869 647 424 248 11
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
SmaI CCC'GGG
Cuts at: 369 1221 369
Size: 852 3347
SmlI C'TyrA_G
Cuts at: 1354 2269 2761 3023 1354
Size: 915 492 262 2530
Fragments arranged by size:
2530 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4199
SphI G_CATG'C
Cuts at: 1252 1752 1252
Size: 500 3699
StyI C'CwwG_G
Cuts at: 107 626 1001 1333 1687 107
Size: 519 375 332 354 2619
Fragments arranged by size:
2619 519 375 354 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 315 485 627 1782 1915 2374 2583 315
Size: 170 142 1155 133 459 209 1931
Fragments arranged by size:
1931 1155 459 209 170 142 133
TatI w'GTAC_w
Cuts at: 302 3714 302
Size: 3412 787
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3716 3748 3716
Size: 32 4167
Tth111I GACn'n_nGTC
Cuts at: 1996 1996
Size: 4199
XbaI T'CTAG_A
Cuts at: 1230 1230
Size: 4199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 213 1694 213
Size: 1481 2718
XmaI C'CCGG_G
Cuts at: 367 1219 367
Size: 852 3347
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI