Restriction map for
pF1KB8812
(Circular) (Six-base) MAPSORT of: pF1KB8812.seq Check: 6637 from: 1 to: 4205
pF1KB8812 4205 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AccI GT'mk_AC
Cuts at: 1243 1243
Size: 4205
Acc65I G'GTAC_C
Cuts at: 402 1135 1221 402
Size: 733 86 3386
Fragments arranged by size:
3386 733 86
AclI AA'CG_TT
Cuts at: 4045 4045
Size: 4205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 2462 3209 3477 2030
Size: 432 747 268 2758
Fragments arranged by size:
2758 747 432 268
AflIII A'CryG_T
Cuts at: 479 937 1557 2661 3638 479
Size: 458 620 1104 977 1046
Fragments arranged by size:
1104 1046 977 620 458
AgeI A'CCGG_T
Cuts at: 2593 2593
Size: 4205
AhdI GACnn_n'nnGTC
Cuts at: 204 204
Size: 4205
AlwNI CAG_nnn'CTG
Cuts at: 882 1510 3077 882
Size: 628 1567 2010
Fragments arranged by size:
2010 1567 628
ApaI G_GGCC'C
Cuts at: 139 908 139
Size: 769 3436
ApaLI G'TGCA_C
Cuts at: 2975 2975
Size: 4205
ApoI r'AATT_y
Cuts at: 1209 3558 3814 1209
Size: 2349 256 1600
Fragments arranged by size:
2349 1600 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4205
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
AvaI C'yCGr_G
Cuts at: 1225 1225
Size: 4205
BamHI G'GATC_C
Cuts at: 237 1230 237
Size: 993 3212
BanI G'GyrC_C
Cuts at: 122 402 421 539 1135 1221 1635 1883 1918
Size: 280 19 118 596 86 414 248 35
Cuts at: 1918 122
Size: 2409
Fragments arranged by size:
2409 596 414 280 248 118 86 35
19
BanII G_rGCy'C
Cuts at: 139 558 617 908 917 1219 2249 139
Size: 419 59 291 9 302 1030 2095
Fragments arranged by size:
2095 1030 419 302 291 59 9
BbeI G_GCGC'C
Cuts at: 543 1639 1887 543
Size: 1096 248 2861
Fragments arranged by size:
2861 1096 248
BbvCI CC'TCA_GC
Cuts at: 728 728
Size: 4205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 121 124 155 90
Size: 31 3 31 4140
Fragments arranged by size:
4140 31 31 3
BciVI GTATCCnnnnn_n'
Cuts at: 2103 2864 4205 2103
Size: 761 1341 2103
Fragments arranged by size:
2103 1341 761
BclI T'GATC_A
Cuts at: 1725 1725
Size: 4205
BglI GCCn_nnn'nGGC
Cuts at: 548 820 548
Size: 272 3933
BglII A'GATC_T
Cuts at: 1720 3757 1720
Size: 2037 2168
BlpI GC'TnA_GC
Cuts at: 1317 1317
Size: 4205
Bme1580I G_kGCm'C
Cuts at: 139 362 908 1830 1923 2979 139
Size: 223 546 922 93 1056 1365
Fragments arranged by size:
1365 1056 922 546 223 93
BmrI ACTGGGnnnn_n'
Cuts at: 913 923 1417 1498 1833 3977 913
Size: 10 494 81 335 2144 1141
Fragments arranged by size:
2144 1141 494 335 81 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 690 690
Size: 4205
Bpu10I CC'TnA_GC
Cuts at: 728 728
Size: 4205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1381 2260 2752 3050 616
Size: 765 879 492 298 1771
Fragments arranged by size:
1771 879 765 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1038 1109 1159 3909 1038
Size: 71 50 2750 1334
Fragments arranged by size:
2750 1334 71 50
BsaAI yAC'GTr
Cuts at: 1558 1558
Size: 4205
BsaBI GATnn'nnATC
Cuts at: 6 1719 3756 6
Size: 1713 2037 455
Fragments arranged by size:
2037 1713 455
BsaHI Gr'CG_yC
Cuts at: 205 540 1636 1884 2586 4082 205
Size: 335 1096 248 702 1496 328
Fragments arranged by size:
1496 1096 702 335 328 248
BsaWI w'CCGG_w
Cuts at: 1394 1915 2593 2867 3014 3425 1394
Size: 521 678 274 147 411 2174
Fragments arranged by size:
2174 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1000 1105 1108 1000
Size: 105 3 4097
Fragments arranged by size:
4097 105 3
BseYI C'CCAG_C
Cuts at: 355 367 398 464 468 977 1053 1630 2388
Size: 12 31 66 4 509 76 577 758
Cuts at: 2388 2965 3461 355
Size: 577 496 1099
Fragments arranged by size:
1099 758 577 577 509 496 76 66
31 12 4
BsiEI CG_ry'CG
Cuts at: 104 1747 1793 3001 104
Size: 1643 46 1208 1308
Fragments arranged by size:
1643 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 307 500 617 867 898 1219 1997 2187 2979
Size: 193 117 250 31 321 778 190 792
Cuts at: 2979 307
Size: 1533
Fragments arranged by size:
1533 792 778 321 250 193 190 117
31
BsmI GAATG_Cn'
Cuts at: 143 143
Size: 4205
BsmBI CGTCTCn'nnnn_
Cuts at: 212 212
Size: 4205
Bsp1286I G_dGCh'C
Cuts at: 139 307 362 500 558 617 867 898 908
Size: 168 55 138 58 59 250 31 10
Cuts at: 908 917 1219 1830 1923 1997 2187 2249 2979
Size: 9 302 611 93 74 190 62 730
Cuts at: 2979 139
Size: 1365
Fragments arranged by size:
1365 730 611 302 250 190 168 138
93 74 62 59 58 55 31 10
9
BspEI T'CCGG_A
Cuts at: 3425 3425
Size: 4205
BspHI T'CATG_A
Cuts at: 3381 3381
Size: 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 342 599 1255 1469 1771 2152 342
Size: 257 656 214 302 381 2395
Fragments arranged by size:
2395 656 381 302 257 214
BsrBI CCG'CTC
Cuts at: 50 252 2497 2551 4035 4200 50
Size: 202 2245 54 1484 165 55
Fragments arranged by size:
2245 1484 202 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2117 2117
Size: 4205
BsrFI r'CCGG_y
Cuts at: 119 2203 2384 2593 119
Size: 2084 181 209 1731
Fragments arranged by size:
2084 1731 209 181
BssSI C'ACGA_G
Cuts at: 113 2476 2834 113
Size: 2363 358 1484
Fragments arranged by size:
2363 1484 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1582 1582
Size: 4205
BstBI TT'CG_AA
Cuts at: 2566 2566
Size: 4205
BstEII G'GTnAC_C
Cuts at: 681 681
Size: 4205
BstYI r'GATC_y
Cuts at: 1 237 530 627 1230 1697 1720 2055 2301
Size: 236 293 97 603 467 23 335 246
Cuts at: 2301 3313 3399 3411 3757 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 603 467 449 346 335 293 246
236 97 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 535 1131 535
Size: 596 3609
BtgI C'CryG_G
Cuts at: 184 290 439 818 184
Size: 106 149 379 3571
Fragments arranged by size:
3571 379 149 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2155 2336 2499 2155
Size: 181 163 3861
Fragments arranged by size:
3861 181 163
BtsI GCAGTG_nn'
Cuts at: 1448 1448
Size: 4205
DraI TTT'AAA
Cuts at: 1204 1204
Size: 4205
DraIII CAC_nnn'GTG
Cuts at: 275 387 275
Size: 112 4093
DrdI GACnn_nn'nnGTC
Cuts at: 1911 2769 1911
Size: 858 3347
EaeI y'GGCC_r
Cuts at: 117 821 1790 1964 2355 2382 3727 117
Size: 704 969 174 391 27 1345 595
Fragments arranged by size:
1345 969 704 595 391 174 27
EagI C'GGCC_G
Cuts at: 1790 1790
Size: 4205
EarI CTCTTCn'nnn_
Cuts at: 295 622 978 2228 2438 295
Size: 327 356 1250 210 2062
Fragments arranged by size:
2062 1250 356 327 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2413 2723 2869 4015 2413
Size: 310 146 1146 2603
Fragments arranged by size:
2603 1146 310 146
EcoICRI GAG'CTC
Cuts at: 615 1217 615
Size: 602 3603
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 690 2030 2462 3209 3477 690
Size: 1340 432 747 268 1418
Fragments arranged by size:
1418 1340 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 135 136 346 588 771 1158 1344 135
Size: 1 210 242 183 387 186 2996
Fragments arranged by size:
2996 387 242 210 186 183 1
EcoRI G'AATT_C
Cuts at: 1209 1209
Size: 4205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 4205
FspI TGC'GCA
Cuts at: 1986 1986
Size: 4205
HaeII r_GCGC'y
Cuts at: 543 1639 1887 2909 3888 543
Size: 1096 248 1022 979 860
Fragments arranged by size:
1096 1022 979 860 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 466 498 2047 2077 2079 2109 190
Size: 32 244 32 1549 30 2 30 2286
Fragments arranged by size:
2286 1549 244 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1244 1244
Size: 4205
KasI G'GCGC_C
Cuts at: 539 1635 1883 539
Size: 1096 248 2861
Fragments arranged by size:
2861 1096 248
KpnI G_GTAC'C
Cuts at: 406 1139 1225 406
Size: 733 86 3386
Fragments arranged by size:
3386 733 86
MluI A'CGCG_T
Cuts at: 3638 3638
Size: 4205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 869 998 2876 3060 869
Size: 129 1878 184 2014
Fragments arranged by size:
2014 1878 184 129
MscI TGG'CCA
Cuts at: 823 1966 823
Size: 1143 3062
MslI CAynn'nnrTG
Cuts at: 183 2194 2321 183
Size: 2011 127 2067
Fragments arranged by size:
2067 2011 127
MspA1I CmG'CkG
Cuts at: 468 714 732 882 1316 1630 1990 3003 3248
Size: 246 18 150 434 314 360 1013 245
Cuts at: 3248 468
Size: 1425
Fragments arranged by size:
1425 1013 434 360 314 246 245 150
18
NaeI GCC'GGC
Cuts at: 2386 2386
Size: 4205
NarI GG'CG_CC
Cuts at: 540 1636 1884 540
Size: 1096 248 2861
Fragments arranged by size:
2861 1096 248
NcoI C'CATG_G
Cuts at: 184 290 818 184
Size: 106 528 3571
Fragments arranged by size:
3571 528 106
NgoMIV G'CCGG_C
Cuts at: 2384 2384
Size: 4205
NspI r_CATG'y
Cuts at: 366 483 941 1258 1758 2665 366
Size: 117 458 317 500 907 1906
Fragments arranged by size:
1906 907 500 458 317 117
PasI CC'CwG_GG
Cuts at: 901 901
Size: 4205
PciI A'CATG_T
Cuts at: 479 937 2661 479
Size: 458 1724 2023
Fragments arranged by size:
2023 1724 458
PflMI CCAn_nnn'nTGG
Cuts at: 387 678 387
Size: 291 3914
PfoI T'CCnGG_A
Cuts at: 1062 1062
Size: 4205
PmeI GTTT'AAAC
Cuts at: 1204 1204
Size: 4205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3344 3376 3344
Size: 32 4173
PpuMI rG'GwC_Cy
Cuts at: 346 588 346
Size: 242 3963
PspOMI G'GGCC_C
Cuts at: 135 904 135
Size: 769 3436
PstI C_TGCA'G
Cuts at: 339 1252 1937 339
Size: 913 685 2607
Fragments arranged by size:
2607 913 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4205
PvuII CAG'CTG
Cuts at: 468 714 732 882 1630 1990 468
Size: 246 18 150 748 360 2683
Fragments arranged by size:
2683 748 360 246 150 18
RsrII CG'GwC_CG
Cuts at: 2400 2400
Size: 4205
SacI G_AGCT'C
Cuts at: 617 1219 617
Size: 602 3603
SalI G'TCGA_C
Cuts at: 1242 1242
Size: 4205
SanDI GG'GwC_CC
Cuts at: 346 346
Size: 4205
SapI GCTCTTCn'nnn_
Cuts at: 295 622 2228 2438 295
Size: 327 1606 210 2062
Fragments arranged by size:
2062 1606 327 210
SbfI CC_TGCA'GG
Cuts at: 1252 1252
Size: 4205
ScaI AGT'ACT
Cuts at: 3722 3722
Size: 4205
SfcI C'TryA_G
Cuts at: 33 335 1248 1933 2926 3117 33
Size: 302 913 685 993 191 1121
Fragments arranged by size:
1121 993 913 685 302 191
SfoI GGC'GCC
Cuts at: 541 1637 1885 541
Size: 1096 248 2861
Fragments arranged by size:
2861 1096 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
SmaI CCC'GGG
Cuts at: 1227 1227
Size: 4205
SmlI C'TyrA_G
Cuts at: 631 1360 2275 2767 3029 631
Size: 729 915 492 262 1807
Fragments arranged by size:
1807 915 729 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4205
SphI G_CATG'C
Cuts at: 1258 1758 1258
Size: 500 3705
StuI AGG'CCT
Cuts at: 562 562
Size: 4205
StyI C'CwwG_G
Cuts at: 184 290 818 887 908 1339 1693 184
Size: 106 528 69 21 431 354 2696
Fragments arranged by size:
2696 528 431 354 106 69 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1788 1921 2380 2589 1788
Size: 133 459 209 3404
Fragments arranged by size:
3404 459 209 133
TatI w'GTAC_w
Cuts at: 3720 3720
Size: 4205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 259 291 3722 3754 259
Size: 32 3431 32 710
Fragments arranged by size:
3431 710 32 32
Tth111I GACn'n_nGTC
Cuts at: 2002 2002
Size: 4205
XbaI T'CTAG_A
Cuts at: 856 1236 856
Size: 380 3825
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1700 1700
Size: 4205
XmaI C'CCGG_G
Cuts at: 1225 1225
Size: 4205
XmnI GAAnn'nnTTC
Cuts at: 641 641
Size: 4205
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BmgBI BmtI BsaXI BsgI
BsiWI BsrGI BssHII BstXI BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI