Restriction map for
pF1KB8817
(Circular) (Six-base) MAPSORT of: pF1KB8817.seq Check: 5609 from: 1 to: 4256
pF1KB8817 4256 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 417 566 1294 417
Size: 149 728 3379
Fragments arranged by size:
3379 728 149
Acc65I G'GTAC_C
Cuts at: 1272 1272
Size: 4256
AclI AA'CG_TT
Cuts at: 4096 4096
Size: 4256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 2081 2513 3260 3528 1027
Size: 1054 432 747 268 1755
Fragments arranged by size:
1755 1054 747 432 268
AflIII A'CryG_T
Cuts at: 1608 2712 3689 1608
Size: 1104 977 2175
Fragments arranged by size:
2175 1104 977
AgeI A'CCGG_T
Cuts at: 2644 2644
Size: 4256
AlwNI CAG_nnn'CTG
Cuts at: 130 501 999 1561 3128 130
Size: 371 498 562 1567 1258
Fragments arranged by size:
1567 1258 562 498 371
ApaLI G'TGCA_C
Cuts at: 3026 3026
Size: 4256
ApoI r'AATT_y
Cuts at: 337 1260 3609 3865 337
Size: 923 2349 256 728
Fragments arranged by size:
2349 923 728 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4256
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
AvaI C'yCGr_G
Cuts at: 1276 1276
Size: 4256
BamHI G'GATC_C
Cuts at: 1281 1281
Size: 4256
BanI G'GyrC_C
Cuts at: 483 1272 1686 1934 1969 483
Size: 789 414 248 35 2770
Fragments arranged by size:
2770 789 414 248 35
BanII G_rGCy'C
Cuts at: 1270 2300 1270
Size: 1030 3226
BbeI G_GCGC'C
Cuts at: 1690 1938 1690
Size: 248 4008
BciVI GTATCCnnnnn_n'
Cuts at: 525 2154 2915 4256 525
Size: 1629 761 1341 525
Fragments arranged by size:
1629 1341 761 525
BclI T'GATC_A
Cuts at: 332 1776 332
Size: 1444 2812
BglI GCCn_nnn'nGGC
Cuts at: 382 643 382
Size: 261 3995
BglII A'GATC_T
Cuts at: 393 584 1771 3808 393
Size: 191 1187 2037 841
Fragments arranged by size:
2037 1187 841 191
BlpI GC'TnA_GC
Cuts at: 1368 1368
Size: 4256
Bme1580I G_kGCm'C
Cuts at: 488 639 1881 1974 3030 488
Size: 151 1242 93 1056 1714
Fragments arranged by size:
1714 1242 1056 151 93
BmrI ACTGGGnnnn_n'
Cuts at: 1468 1549 1884 4028 1468
Size: 81 335 2144 1696
Fragments arranged by size:
2144 1696 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 4256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 884 1216 884
Size: 332 3924
Bpu10I CC'TnA_GC
Cuts at: 215 215
Size: 4256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1432 2311 2803 3101 1432
Size: 879 492 298 2587
Fragments arranged by size:
2587 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3960 3960
Size: 4256
BsaAI yAC'GTr
Cuts at: 1609 1609
Size: 4256
BsaBI GATnn'nnATC
Cuts at: 6 685 1770 3807 6
Size: 679 1085 2037 455
Fragments arranged by size:
2037 1085 679 455
BsaHI Gr'CG_yC
Cuts at: 1687 1935 2637 4133 1687
Size: 248 702 1496 1810
Fragments arranged by size:
1810 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1445 1966 2644 2918 3065 3476 1445
Size: 521 678 274 147 411 2225
Fragments arranged by size:
2225 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 751 751
Size: 4256
BseYI C'CCAG_C
Cuts at: 1681 2439 3016 3512 1681
Size: 758 577 496 2425
Fragments arranged by size:
2425 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 143
Size: 4256
BsiEI CG_ry'CG
Cuts at: 104 1798 1844 3052 104
Size: 1694 46 1208 1308
Fragments arranged by size:
1694 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1057 1270 2048 2238 3030 1057
Size: 213 778 190 792 2283
Fragments arranged by size:
2283 792 778 213 190
Bsp1286I G_dGCh'C
Cuts at: 488 639 1057 1270 1881 1974 2048 2238 2300
Size: 151 418 213 611 93 74 190 62
Cuts at: 2300 3030 488
Size: 730 1714
Fragments arranged by size:
1714 730 611 418 213 190 151 93
74 62
BspEI T'CCGG_A
Cuts at: 3476 3476
Size: 4256
BspHI T'CATG_A
Cuts at: 3432 3432
Size: 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1306 1520 1822 2203 1306
Size: 214 302 381 3359
Fragments arranged by size:
3359 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2548 2602 4086 4251 50
Size: 2498 54 1484 165 55
Fragments arranged by size:
2498 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 371 2168 371
Size: 1797 2459
BsrFI r'CCGG_y
Cuts at: 548 2254 2435 2644 548
Size: 1706 181 209 2160
Fragments arranged by size:
2160 1706 209 181
BssSI C'ACGA_G
Cuts at: 2527 2885 2527
Size: 358 3898
BstAPI GCAn_nnn'nTGC
Cuts at: 1633 1633
Size: 4256
BstBI TT'CG_AA
Cuts at: 2617 2617
Size: 4256
BstXI CCAn_nnnn'nTGG
Cuts at: 915 915
Size: 4256
BstYI r'GATC_y
Cuts at: 1 393 584 1281 1748 1771 2106 2352 3364
Size: 392 191 697 467 23 335 246 1012
Cuts at: 3364 3450 3462 3808 1
Size: 86 12 346 449
Fragments arranged by size:
1012 697 467 449 392 346 335 246
191 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2206 2387 2550 2206
Size: 181 163 3912
Fragments arranged by size:
3912 181 163
BtsI GCAGTG_nn'
Cuts at: 1499 1499
Size: 4256
DraI TTT'AAA
Cuts at: 1255 1255
Size: 4256
DraIII CAC_nnn'GTG
Cuts at: 413 483 413
Size: 70 4186
DrdI GACnn_nn'nnGTC
Cuts at: 994 1962 2820 994
Size: 968 858 2430
Fragments arranged by size:
2430 968 858
EaeI y'GGCC_r
Cuts at: 656 1841 2015 2406 2433 3778 656
Size: 1185 174 391 27 1345 1134
Fragments arranged by size:
1345 1185 1134 391 174 27
EagI C'GGCC_G
Cuts at: 1841 1841
Size: 4256
EarI CTCTTCn'nnn_
Cuts at: 736 2279 2489 736
Size: 1543 210 2503
Fragments arranged by size:
2503 1543 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2464 2774 2920 4066 2464
Size: 310 146 1146 2654
Fragments arranged by size:
2654 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1268 1268
Size: 4256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 884 1027 1216 2081 2513 3260 3528 884
Size: 143 189 865 432 747 268 1612
Fragments arranged by size:
1612 865 747 432 268 189 143
EcoO109I rG'GnC_Cy
Cuts at: 463 498 996 1395 463
Size: 35 498 399 3324
Fragments arranged by size:
3324 498 399 35
EcoRI G'AATT_C
Cuts at: 1260 1260
Size: 4256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2275 2275
Size: 4256
FspI TGC'GCA
Cuts at: 352 2037 352
Size: 1685 2571
FspAI rTGC'GCAy
Cuts at: 352 352
Size: 4256
HaeII r_GCGC'y
Cuts at: 1690 1938 2960 3939 1690
Size: 248 1022 979 2007
Fragments arranged by size:
2007 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2098 2128 2130 2160 2098
Size: 30 2 30 4194
Fragments arranged by size:
4194 30 30 2
HincII GTy'rAC
Cuts at: 981 1295 981
Size: 314 3942
KasI G'GCGC_C
Cuts at: 1686 1934 1686
Size: 248 4008
KpnI G_GTAC'C
Cuts at: 1276 1276
Size: 4256
MluI A'CGCG_T
Cuts at: 3689 3689
Size: 4256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1124 2927 3111 1124
Size: 1803 184 2269
Fragments arranged by size:
2269 1803 184
MscI TGG'CCA
Cuts at: 658 2017 658
Size: 1359 2897
MslI CAynn'nnrTG
Cuts at: 910 2245 2372 910
Size: 1335 127 2794
Fragments arranged by size:
2794 1335 127
MspA1I CmG'CkG
Cuts at: 947 1130 1140 1367 1681 2041 3054 3299 947
Size: 183 10 227 314 360 1013 245 1904
Fragments arranged by size:
1904 1013 360 314 245 227 183 10
NaeI GCC'GGC
Cuts at: 2437 2437
Size: 4256
NarI GG'CG_CC
Cuts at: 1687 1935 1687
Size: 248 4008
NcoI C'CATG_G
Cuts at: 107 107
Size: 4256
NgoMIV G'CCGG_C
Cuts at: 2435 2435
Size: 4256
NspI r_CATG'y
Cuts at: 351 1309 1809 2716 351
Size: 958 500 907 1891
Fragments arranged by size:
1891 958 907 500
PasI CC'CwG_GG
Cuts at: 495 495
Size: 4256
PciI A'CATG_T
Cuts at: 2712 2712
Size: 4256
PflMI CCAn_nnn'nTGG
Cuts at: 147 501 999 147
Size: 354 498 3404
Fragments arranged by size:
3404 498 354
PfoI T'CCnGG_A
Cuts at: 665 692 861 998 665
Size: 27 169 137 3923
Fragments arranged by size:
3923 169 137 27
PmeI GTTT'AAAC
Cuts at: 1255 1255
Size: 4256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 4224
PpuMI rG'GwC_Cy
Cuts at: 463 996 463
Size: 533 3723
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 245 277 1084 1116 245
Size: 32 807 32 3385
Fragments arranged by size:
3385 807 32 32
PstI C_TGCA'G
Cuts at: 1303 1988 1303
Size: 685 3571
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4256
PvuII CAG'CTG
Cuts at: 947 1681 2041 947
Size: 734 360 3162
Fragments arranged by size:
3162 734 360
RsrII CG'GwC_CG
Cuts at: 2451 2451
Size: 4256
SacI G_AGCT'C
Cuts at: 1270 1270
Size: 4256
SalI G'TCGA_C
Cuts at: 1293 1293
Size: 4256
SapI GCTCTTCn'nnn_
Cuts at: 736 2279 2489 736
Size: 1543 210 2503
Fragments arranged by size:
2503 1543 210
SbfI CC_TGCA'GG
Cuts at: 1303 1303
Size: 4256
ScaI AGT'ACT
Cuts at: 3773 3773
Size: 4256
SexAI A'CCwGG_T
Cuts at: 479 479
Size: 4256
SfcI C'TryA_G
Cuts at: 33 762 1101 1299 1984 2977 3168 33
Size: 729 339 198 685 993 191 1121
Fragments arranged by size:
1121 993 729 685 339 198 191
SfoI GGC'GCC
Cuts at: 1688 1936 1688
Size: 248 4008
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
SmaI CCC'GGG
Cuts at: 1278 1278
Size: 4256
SmlI C'TyrA_G
Cuts at: 1411 2326 2818 3080 1411
Size: 915 492 262 2587
Fragments arranged by size:
2587 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4256
SphI G_CATG'C
Cuts at: 351 1309 1809 351
Size: 958 500 2798
Fragments arranged by size:
2798 958 500
StyI C'CwwG_G
Cuts at: 107 314 1390 1744 107
Size: 207 1076 354 2619
Fragments arranged by size:
2619 1076 354 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1839 1972 2431 2640 1839
Size: 133 459 209 3455
Fragments arranged by size:
3455 459 209 133
TatI w'GTAC_w
Cuts at: 507 715 1013 3771 507
Size: 208 298 2758 992
Fragments arranged by size:
2758 992 298 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3773 3805 3773
Size: 32 4224
Tth111I GACn'n_nGTC
Cuts at: 2053 2053
Size: 4256
XbaI T'CTAG_A
Cuts at: 1287 1287
Size: 4256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 384 859 912 1751 384
Size: 475 53 839 2889
Fragments arranged by size:
2889 839 475 53
XmaI C'CCGG_G
Cuts at: 1276 1276
Size: 4256
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspOMI PspXI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI