Restriction map for
pF1KB8819
(Circular) (Six-base) MAPSORT of: pF1KB8819.seq Check: 7720 from: 1 to: 4259
pF1KB8819 4259 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1238 1238
Size: 4259
AccI GT'mk_AC
Cuts at: 1297 1297
Size: 4259
Acc65I G'GTAC_C
Cuts at: 1275 1275
Size: 4259
AclI AA'CG_TT
Cuts at: 4099 4099
Size: 4259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1081 2084 2516 3263 3531 772
Size: 309 1003 432 747 268 1500
Fragments arranged by size:
1500 1003 747 432 309 268
AflII C'TTAA_G
Cuts at: 405 405
Size: 4259
AflIII A'CryG_T
Cuts at: 1611 2715 3692 1611
Size: 1104 977 2178
Fragments arranged by size:
2178 1104 977
AgeI A'CCGG_T
Cuts at: 2647 2647
Size: 4259
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 490 522 490
Size: 32 4227
AlwNI CAG_nnn'CTG
Cuts at: 1564 3131 1564
Size: 1567 2692
ApaI G_GGCC'C
Cuts at: 471 471
Size: 4259
ApaLI G'TGCA_C
Cuts at: 3029 3029
Size: 4259
ApoI r'AATT_y
Cuts at: 345 649 1069 1263 3612 3868 345
Size: 304 420 194 2349 256 736
Fragments arranged by size:
2349 736 420 304 256 194
AseI AT'TA_AT
Cuts at: 20 359 20
Size: 339 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
AvaI C'yCGr_G
Cuts at: 1279 1279
Size: 4259
BamHI G'GATC_C
Cuts at: 1284 1284
Size: 4259
BanI G'GyrC_C
Cuts at: 1011 1275 1689 1937 1972 1011
Size: 264 414 248 35 3298
Fragments arranged by size:
3298 414 264 248 35
BanII G_rGCy'C
Cuts at: 471 1210 1273 2303 471
Size: 739 63 1030 2427
Fragments arranged by size:
2427 1030 739 63
BbeI G_GCGC'C
Cuts at: 1693 1941 1693
Size: 248 4011
BbvCI CC'TCA_GC
Cuts at: 671 979 671
Size: 308 3951
BciVI GTATCCnnnnn_n'
Cuts at: 2157 2918 4259 2157
Size: 761 1341 2157
Fragments arranged by size:
2157 1341 761
BclI T'GATC_A
Cuts at: 1779 1779
Size: 4259
BglI GCCn_nnn'nGGC
Cuts at: 284 868 284
Size: 584 3675
BglII A'GATC_T
Cuts at: 1774 3811 1774
Size: 2037 2222
BlpI GC'TnA_GC
Cuts at: 1371 1371
Size: 4259
Bme1580I G_kGCm'C
Cuts at: 471 1884 1977 3033 471
Size: 1413 93 1056 1697
Fragments arranged by size:
1697 1413 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1471 1552 1887 4031 1471
Size: 81 335 2144 1699
Fragments arranged by size:
2144 1699 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 631 2626 631
Size: 1995 2264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 437
Size: 4259
Bpu10I CC'TnA_GC
Cuts at: 671 979 671
Size: 308 3951
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 1435 2314 2806 3104 139
Size: 1296 879 492 298 1294
Fragments arranged by size:
1296 1294 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 392 3963 392
Size: 3571 688
BsaAI yAC'GTr
Cuts at: 171 1612 171
Size: 1441 2818
BsaBI GATnn'nnATC
Cuts at: 6 1773 3810 6
Size: 1767 2037 455
Fragments arranged by size:
2037 1767 455
BsaHI Gr'CG_yC
Cuts at: 1690 1938 2640 4136 1690
Size: 248 702 1496 1813
Fragments arranged by size:
1813 1496 702 248
BsaWI w'CCGG_w
Cuts at: 264 1448 1969 2647 2921 3068 3479 264
Size: 1184 521 678 274 147 411 1044
Fragments arranged by size:
1184 1044 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1684 2442 3019 3515 1684
Size: 758 577 496 2428
Fragments arranged by size:
2428 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1801 1847 3055 104
Size: 1697 46 1208 1308
Fragments arranged by size:
1697 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 930 1273 2051 2241 3033 930
Size: 343 778 190 792 2156
Fragments arranged by size:
2156 792 778 343 190
BsmBI CGTCTCn'nnnn_
Cuts at: 722 722
Size: 4259
Bsp1286I G_dGCh'C
Cuts at: 471 930 1210 1273 1884 1977 2051 2241 2303
Size: 459 280 63 611 93 74 190 62
Cuts at: 2303 3033 471
Size: 730 1697
Fragments arranged by size:
1697 730 611 459 280 190 93 74
63 62
BspEI T'CCGG_A
Cuts at: 3479 3479
Size: 4259
BspHI T'CATG_A
Cuts at: 3435 3435
Size: 4259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1238 1309 1523 1825 2206 1238
Size: 71 214 302 381 3291
Fragments arranged by size:
3291 381 302 214 71
BsrBI CCG'CTC
Cuts at: 50 116 163 663 2551 2605 4089 4254 50
Size: 66 47 500 1888 54 1484 165 55
Fragments arranged by size:
1888 1484 500 165 66 55 54 47
BsrDI GCAATG_nn'
Cuts at: 2171 2171
Size: 4259
BsrFI r'CCGG_y
Cuts at: 2257 2438 2647 2257
Size: 181 209 3869
Fragments arranged by size:
3869 209 181
BssSI C'ACGA_G
Cuts at: 853 2530 2888 853
Size: 1677 358 2224
Fragments arranged by size:
2224 1677 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1636 1636
Size: 4259
BstBI TT'CG_AA
Cuts at: 2620 2620
Size: 4259
BstEII G'GTnAC_C
Cuts at: 267 267
Size: 4259
BstXI CCAn_nnnn'nTGG
Cuts at: 1179 1179
Size: 4259
BstYI r'GATC_y
Cuts at: 1 1284 1751 1774 2109 2355 3367 3453 3465
Size: 1283 467 23 335 246 1012 86 12
Cuts at: 3465 3811 1
Size: 346 449
Fragments arranged by size:
1283 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 314 107
Size: 207 4052
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 539 2209 2390 2553 539
Size: 1670 181 163 2245
Fragments arranged by size:
2245 1670 181 163
BtsI GCAGTG_nn'
Cuts at: 1184 1502 1184
Size: 318 3941
ClaI AT'CG_AT
Cuts at: 899 899
Size: 4259
DraI TTT'AAA
Cuts at: 1258 1258
Size: 4259
DrdI GACnn_nn'nnGTC
Cuts at: 190 1965 2823 190
Size: 1775 858 1626
Fragments arranged by size:
1775 1626 858
EaeI y'GGCC_r
Cuts at: 572 1194 1844 2018 2409 2436 3781 572
Size: 622 650 174 391 27 1345 1050
Fragments arranged by size:
1345 1050 650 622 391 174 27
EagI C'GGCC_G
Cuts at: 1844 1844
Size: 4259
EarI CTCTTCn'nnn_
Cuts at: 653 1157 2282 2492 653
Size: 504 1125 210 2420
Fragments arranged by size:
2420 1125 504 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 364 978 2467 2777 2923 4069 364
Size: 614 1489 310 146 1146 554
Fragments arranged by size:
1489 1146 614 554 310 146
EcoICRI GAG'CTC
Cuts at: 1271 1271
Size: 4259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 772 1081 2084 2516 3263 3531 437
Size: 335 309 1003 432 747 268 1165
Fragments arranged by size:
1165 1003 747 432 335 309 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1234 1234
Size: 4259
EcoO109I rG'GnC_Cy
Cuts at: 467 468 1398 467
Size: 1 930 3328
Fragments arranged by size:
3328 930 1
EcoRI G'AATT_C
Cuts at: 1263 1263
Size: 4259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 4259
FspI TGC'GCA
Cuts at: 2040 2040
Size: 4259
HaeII r_GCGC'y
Cuts at: 1693 1941 2963 3942 1693
Size: 248 1022 979 2010
Fragments arranged by size:
2010 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 520 552 2101 2131 2133 2163 520
Size: 32 1549 30 2 30 2616
Fragments arranged by size:
2616 1549 32 30 30 2
HincII GTy'rAC
Cuts at: 300 1298 300
Size: 998 3261
HpaI GTT'AAC
Cuts at: 300 300
Size: 4259
KasI G'GCGC_C
Cuts at: 1689 1937 1689
Size: 248 4011
KpnI G_GTAC'C
Cuts at: 1279 1279
Size: 4259
MluI A'CGCG_T
Cuts at: 3692 3692
Size: 4259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2930 3114 2930
Size: 184 4075
MscI TGG'CCA
Cuts at: 2020 2020
Size: 4259
MslI CAynn'nnrTG
Cuts at: 602 2248 2375 602
Size: 1646 127 2486
Fragments arranged by size:
2486 1646 127
MspA1I CmG'CkG
Cuts at: 316 577 1370 1684 2044 3057 3302 316
Size: 261 793 314 360 1013 245 1273
Fragments arranged by size:
1273 1013 793 360 314 261 245
NaeI GCC'GGC
Cuts at: 2440 2440
Size: 4259
NarI GG'CG_CC
Cuts at: 1690 1938 1690
Size: 248 4011
NcoI C'CATG_G
Cuts at: 107 107
Size: 4259
NgoMIV G'CCGG_C
Cuts at: 2438 2438
Size: 4259
NspI r_CATG'y
Cuts at: 207 1225 1312 1812 2719 207
Size: 1018 87 500 907 1747
Fragments arranged by size:
1747 1018 907 500 87
PasI CC'CwG_GG
Cuts at: 282 1006 1230 282
Size: 724 224 3311
Fragments arranged by size:
3311 724 224
PciI A'CATG_T
Cuts at: 2715 2715
Size: 4259
PmeI GTTT'AAAC
Cuts at: 1258 1258
Size: 4259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 3398
Size: 32 4227
PspOMI G'GGCC_C
Cuts at: 467 467
Size: 4259
PstI C_TGCA'G
Cuts at: 1306 1991 1306
Size: 685 3574
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4259
PvuII CAG'CTG
Cuts at: 1684 2044 1684
Size: 360 3899
RsrII CG'GwC_CG
Cuts at: 2454 2454
Size: 4259
SacI G_AGCT'C
Cuts at: 1273 1273
Size: 4259
SacII CC_GC'GG
Cuts at: 317 317
Size: 4259
SalI G'TCGA_C
Cuts at: 1296 1296
Size: 4259
SapI GCTCTTCn'nnn_
Cuts at: 653 2282 2492 653
Size: 1629 210 2420
Fragments arranged by size:
2420 1629 210
SbfI CC_TGCA'GG
Cuts at: 1306 1306
Size: 4259
ScaI AGT'ACT
Cuts at: 3776 3776
Size: 4259
SfcI C'TryA_G
Cuts at: 33 1302 1987 2980 3171 33
Size: 1269 685 993 191 1121
Fragments arranged by size:
1269 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1691 1939 1691
Size: 248 4011
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
SmaI CCC'GGG
Cuts at: 1281 1281
Size: 4259
SmlI C'TyrA_G
Cuts at: 154 405 1414 2329 2821 3083 154
Size: 251 1009 915 492 262 1330
Fragments arranged by size:
1330 1009 915 492 262 251
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4259
SphI G_CATG'C
Cuts at: 1312 1812 1312
Size: 500 3759
StuI AGG'CCT
Cuts at: 225 225
Size: 4259
StyI C'CwwG_G
Cuts at: 107 1393 1747 107
Size: 1286 354 2619
Fragments arranged by size:
2619 1286 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 163 1842 1975 2434 2643 163
Size: 1679 133 459 209 1779
Fragments arranged by size:
1779 1679 459 209 133
TatI w'GTAC_w
Cuts at: 3774 3774
Size: 4259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 310 342 1124 1156 3776 3808 310
Size: 32 782 32 2620 32 761
Fragments arranged by size:
2620 782 761 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2056 2056
Size: 4259
XbaI T'CTAG_A
Cuts at: 1290 1290
Size: 4259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 634 1107 1754 634
Size: 473 647 3139
Fragments arranged by size:
3139 647 473
XmaI C'CCGG_G
Cuts at: 1279 1279
Size: 4259
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AscI AvrII BaeI
BbsI BcgI BfrBI BmgBI BmtI BsaXI BseRI BsgI
BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I CspCI DraIII
EcoRV FseI FspAI HindIII MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI