Restriction map for
pF1KB8820
(Circular) (Six-base) MAPSORT of: pF1KB8820.seq Check: 5617 from: 1 to: 4265
pF1KB8820 4265 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1303 1303
Size: 4265
Acc65I G'GTAC_C
Cuts at: 1281 1281
Size: 4265
AclI AA'CG_TT
Cuts at: 4105 4105
Size: 4265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 2090 2522 3269 3537 1096
Size: 994 432 747 268 1824
Fragments arranged by size:
1824 994 747 432 268
AflIII A'CryG_T
Cuts at: 1617 2721 3698 1617
Size: 1104 977 2184
Fragments arranged by size:
2184 1104 977
AgeI A'CCGG_T
Cuts at: 2653 2653
Size: 4265
AlwNI CAG_nnn'CTG
Cuts at: 1074 1570 3137 1074
Size: 496 1567 2202
Fragments arranged by size:
2202 1567 496
ApaLI G'TGCA_C
Cuts at: 3035 3035
Size: 4265
ApoI r'AATT_y
Cuts at: 1269 3618 3874 1269
Size: 2349 256 1660
Fragments arranged by size:
2349 1660 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4265
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
AvaI C'yCGr_G
Cuts at: 578 1285 578
Size: 707 3558
AvrII C'CTAG_G
Cuts at: 756 756
Size: 4265
BamHI G'GATC_C
Cuts at: 1290 1290
Size: 4265
BanI G'GyrC_C
Cuts at: 1106 1113 1281 1695 1943 1978 1106
Size: 7 168 414 248 35 3393
Fragments arranged by size:
3393 414 248 168 35 7
BanII G_rGCy'C
Cuts at: 1279 2309 1279
Size: 1030 3235
BbeI G_GCGC'C
Cuts at: 1699 1947 1699
Size: 248 4017
BbsI GAAGACnn'nnnn_
Cuts at: 240 252 432 240
Size: 12 180 4073
Fragments arranged by size:
4073 180 12
BbvCI CC'TCA_GC
Cuts at: 289 571 826 289
Size: 282 255 3728
Fragments arranged by size:
3728 282 255
BciVI GTATCCnnnnn_n'
Cuts at: 2163 2924 4265 2163
Size: 761 1341 2163
Fragments arranged by size:
2163 1341 761
BclI T'GATC_A
Cuts at: 1785 1785
Size: 4265
BglI GCCn_nnn'nGGC
Cuts at: 339 339
Size: 4265
BglII A'GATC_T
Cuts at: 353 1780 3817 353
Size: 1427 2037 801
Fragments arranged by size:
2037 1427 801
BlpI GC'TnA_GC
Cuts at: 554 1377 554
Size: 823 3442
Bme1580I G_kGCm'C
Cuts at: 1890 1983 3039 1890
Size: 93 1056 3116
Fragments arranged by size:
3116 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 250 1477 1558 1893 4037 250
Size: 1227 81 335 2144 478
Fragments arranged by size:
2144 1227 478 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2632 2632
Size: 4265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 973 1035 559
Size: 414 62 3789
Fragments arranged by size:
3789 414 62
Bpu10I CC'TnA_GC
Cuts at: 289 571 826 289
Size: 282 255 3728
Fragments arranged by size:
3728 282 255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1441 2320 2812 3110 1441
Size: 879 492 298 2596
Fragments arranged by size:
2596 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3969 3969
Size: 4265
BsaAI yAC'GTr
Cuts at: 330 1618 330
Size: 1288 2977
BsaBI GATnn'nnATC
Cuts at: 6 1779 3816 6
Size: 1773 2037 455
Fragments arranged by size:
2037 1773 455
BsaHI Gr'CG_yC
Cuts at: 1696 1944 2646 4142 1696
Size: 248 702 1496 1819
Fragments arranged by size:
1819 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1454 1975 2653 2927 3074 3485 1454
Size: 521 678 274 147 411 2234
Fragments arranged by size:
2234 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 108 138 108
Size: 30 4235
BseYI C'CCAG_C
Cuts at: 1690 2448 3025 3521 1690
Size: 758 577 496 2434
Fragments arranged by size:
2434 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1807 1853 3061 104
Size: 1703 46 1208 1308
Fragments arranged by size:
1703 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 472 1279 2057 2247 3039 472
Size: 807 778 190 792 1698
Fragments arranged by size:
1698 807 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 636 636
Size: 4265
Bsp1286I G_dGCh'C
Cuts at: 472 1279 1890 1983 2057 2247 2309 3039 472
Size: 807 611 93 74 190 62 730 1698
Fragments arranged by size:
1698 807 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3485 3485
Size: 4265
BspHI T'CATG_A
Cuts at: 3441 3441
Size: 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 947 1315 1529 1831 2212 947
Size: 368 214 302 381 3000
Fragments arranged by size:
3000 381 368 302 214
BsrBI CCG'CTC
Cuts at: 50 2557 2611 4095 4260 50
Size: 2507 54 1484 165 55
Fragments arranged by size:
2507 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2177 2177
Size: 4265
BsrFI r'CCGG_y
Cuts at: 2263 2444 2653 2263
Size: 181 209 3875
Fragments arranged by size:
3875 209 181
BsrGI T'GTAC_A
Cuts at: 654 654
Size: 4265
BssSI C'ACGA_G
Cuts at: 2536 2894 2536
Size: 358 3907
BstAPI GCAn_nnn'nTGC
Cuts at: 1642 1642
Size: 4265
BstBI TT'CG_AA
Cuts at: 2626 2626
Size: 4265
BstYI r'GATC_y
Cuts at: 1 353 1290 1757 1780 2115 2361 3373 3459
Size: 352 937 467 23 335 246 1012 86
Cuts at: 3459 3471 3817 1
Size: 12 346 449
Fragments arranged by size:
1012 937 467 449 352 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 422 107
Size: 315 3950
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 990 2215 2396 2559 990
Size: 1225 181 163 2696
Fragments arranged by size:
2696 1225 181 163
BtsI GCAGTG_nn'
Cuts at: 116 233 400 1017 1121 1508 116
Size: 117 167 617 104 387 2873
Fragments arranged by size:
2873 617 387 167 117 104
DraI TTT'AAA
Cuts at: 854 1264 854
Size: 410 3855
DrdI GACnn_nn'nnGTC
Cuts at: 1971 2829 1971
Size: 858 3407
EaeI y'GGCC_r
Cuts at: 110 371 626 1850 2024 2415 2442 3787 110
Size: 261 255 1224 174 391 27 1345 588
Fragments arranged by size:
1345 1224 588 391 261 255 174 27
EagI C'GGCC_G
Cuts at: 1850 1850
Size: 4265
EarI CTCTTCn'nnn_
Cuts at: 736 2288 2498 736
Size: 1552 210 2503
Fragments arranged by size:
2503 1552 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 357 1054 2473 2783 2929 4075 357
Size: 697 1419 310 146 1146 547
Fragments arranged by size:
1419 1146 697 547 310 146
EcoICRI GAG'CTC
Cuts at: 1277 1277
Size: 4265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 973 1035 1096 2090 2522 3269 3537 559
Size: 414 62 61 994 432 747 268 1287
Fragments arranged by size:
1287 994 747 432 414 268 62 61
EcoNI CCTnn'n_nnAGG
Cuts at: 564 564
Size: 4265
EcoO109I rG'GnC_Cy
Cuts at: 575 1404 575
Size: 829 3436
EcoRI G'AATT_C
Cuts at: 1269 1269
Size: 4265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 4265
FspI TGC'GCA
Cuts at: 2046 2046
Size: 4265
HaeII r_GCGC'y
Cuts at: 1699 1947 2969 3948 1699
Size: 248 1022 979 2016
Fragments arranged by size:
2016 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1198 1230 2107 2137 2139 2169 1198
Size: 32 877 30 2 30 3294
Fragments arranged by size:
3294 877 32 30 30 2
HincII GTy'rAC
Cuts at: 1304 1304
Size: 4265
KasI G'GCGC_C
Cuts at: 1695 1943 1695
Size: 248 4017
KpnI G_GTAC'C
Cuts at: 1285 1285
Size: 4265
MluI A'CGCG_T
Cuts at: 3698 3698
Size: 4265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 404 914 2936 3120 404
Size: 510 2022 184 1549
Fragments arranged by size:
2022 1549 510 184
MscI TGG'CCA
Cuts at: 373 2026 373
Size: 1653 2612
MslI CAynn'nnrTG
Cuts at: 421 2254 2381 421
Size: 1833 127 2305
Fragments arranged by size:
2305 1833 127
MspA1I CmG'CkG
Cuts at: 293 1145 1376 1690 2050 3063 3308 293
Size: 852 231 314 360 1013 245 1250
Fragments arranged by size:
1250 1013 852 360 314 245 231
NaeI GCC'GGC
Cuts at: 2446 2446
Size: 4265
NarI GG'CG_CC
Cuts at: 1696 1944 1696
Size: 248 4017
NcoI C'CATG_G
Cuts at: 107 107
Size: 4265
NgoMIV G'CCGG_C
Cuts at: 2444 2444
Size: 4265
NspI r_CATG'y
Cuts at: 1318 1818 2725 1318
Size: 500 907 2858
Fragments arranged by size:
2858 907 500
PciI A'CATG_T
Cuts at: 2721 2721
Size: 4265
PflMI CCAn_nnn'nTGG
Cuts at: 611 611
Size: 4265
PmeI GTTT'AAAC
Cuts at: 1264 1264
Size: 4265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 4233
PpuMI rG'GwC_Cy
Cuts at: 575 575
Size: 4265
PstI C_TGCA'G
Cuts at: 217 867 1312 1997 217
Size: 650 445 685 2485
Fragments arranged by size:
2485 685 650 445
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4265
PvuII CAG'CTG
Cuts at: 1145 1690 2050 1145
Size: 545 360 3360
Fragments arranged by size:
3360 545 360
RsrII CG'GwC_CG
Cuts at: 2460 2460
Size: 4265
SacI G_AGCT'C
Cuts at: 1279 1279
Size: 4265
SalI G'TCGA_C
Cuts at: 1302 1302
Size: 4265
SapI GCTCTTCn'nnn_
Cuts at: 736 2288 2498 736
Size: 1552 210 2503
Fragments arranged by size:
2503 1552 210
SbfI CC_TGCA'GG
Cuts at: 1312 1312
Size: 4265
ScaI AGT'ACT
Cuts at: 3782 3782
Size: 4265
SfcI C'TryA_G
Cuts at: 33 213 264 863 1308 1993 2986 3177 33
Size: 180 51 599 445 685 993 191 1121
Fragments arranged by size:
1121 993 685 599 445 191 180 51
SfoI GGC'GCC
Cuts at: 1697 1945 1697
Size: 248 4017
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
SmaI CCC'GGG
Cuts at: 580 1287 580
Size: 707 3558
SmlI C'TyrA_G
Cuts at: 1420 2335 2827 3089 1420
Size: 915 492 262 2596
Fragments arranged by size:
2596 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4265
SphI G_CATG'C
Cuts at: 1318 1818 1318
Size: 500 3765
StuI AGG'CCT
Cuts at: 1132 1132
Size: 4265
StyI C'CwwG_G
Cuts at: 107 674 756 869 1200 1399 1753 107
Size: 567 82 113 331 199 354 2619
Fragments arranged by size:
2619 567 354 331 199 113 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1848 1981 2440 2649 1848
Size: 133 459 209 3464
Fragments arranged by size:
3464 459 209 133
TatI w'GTAC_w
Cuts at: 654 3780 654
Size: 3126 1139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 108 140 3782 3814 108
Size: 32 3642 32 559
Fragments arranged by size:
3642 559 32 32
Tth111I GACn'n_nGTC
Cuts at: 2062 2062
Size: 4265
XbaI T'CTAG_A
Cuts at: 975 1296 975
Size: 321 3944
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 642 1760 642
Size: 1118 3147
XmaI C'CCGG_G
Cuts at: 578 1285 578
Size: 707 3558
XmnI GAAnn'nnTTC
Cuts at: 730 1081 730
Size: 351 3914
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BcgI BfrBI BmgBI BmtI BseRI
BsgI BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI