Restriction map for
pF1KB8822
(Circular) (Six-base) MAPSORT of: pF1KB8822.seq Check: 7691 from: 1 to: 4301
pF1KB8822 4301 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 365 616 365
Size: 251 4050
AccI GT'mk_AC
Cuts at: 1339 1339
Size: 4301
Acc65I G'GTAC_C
Cuts at: 591 1317 591
Size: 726 3575
AclI AA'CG_TT
Cuts at: 4141 4141
Size: 4301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 2126 2558 3305 3573 310
Size: 1816 432 747 268 1038
Fragments arranged by size:
1816 1038 747 432 268
AflIII A'CryG_T
Cuts at: 1653 2757 3734 1653
Size: 1104 977 2220
Fragments arranged by size:
2220 1104 977
AgeI A'CCGG_T
Cuts at: 370 2689 370
Size: 2319 1982
AlwNI CAG_nnn'CTG
Cuts at: 741 828 906 1606 3173 741
Size: 87 78 700 1567 1869
Fragments arranged by size:
1869 1567 700 87 78
ApaI G_GGCC'C
Cuts at: 1274 1274
Size: 4301
ApaLI G'TGCA_C
Cuts at: 3071 3071
Size: 4301
ApoI r'AATT_y
Cuts at: 1045 1305 3654 3910 1045
Size: 260 2349 256 1436
Fragments arranged by size:
2349 1436 260 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4301
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
AvaI C'yCGr_G
Cuts at: 1151 1321 1151
Size: 170 4131
BamHI G'GATC_C
Cuts at: 1326 1326
Size: 4301
BanI G'GyrC_C
Cuts at: 208 373 591 1317 1731 1979 2014 208
Size: 165 218 726 414 248 35 2495
Fragments arranged by size:
2495 726 414 248 218 165 35
BanII G_rGCy'C
Cuts at: 401 680 1029 1274 1315 2345 401
Size: 279 349 245 41 1030 2357
Fragments arranged by size:
2357 1030 349 279 245 41
BbeI G_GCGC'C
Cuts at: 1735 1983 1735
Size: 248 4053
BciVI GTATCCnnnnn_n'
Cuts at: 2199 2960 4301 2199
Size: 761 1341 2199
Fragments arranged by size:
2199 1341 761
BclI T'GATC_A
Cuts at: 1821 1821
Size: 4301
BglII A'GATC_T
Cuts at: 1816 3853 1816
Size: 2037 2264
BlpI GC'TnA_GC
Cuts at: 679 956 1413 679
Size: 277 457 3567
Fragments arranged by size:
3567 457 277
Bme1580I G_kGCm'C
Cuts at: 1274 1926 2019 3075 1274
Size: 652 93 1056 2500
Fragments arranged by size:
2500 1056 652 93
BmrI ACTGGGnnnn_n'
Cuts at: 463 851 1513 1594 1929 4073 463
Size: 388 662 81 335 2144 691
Fragments arranged by size:
2144 691 662 388 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 965 965
Size: 4301
Bpu10I CC'TnA_GC
Cuts at: 482 1096 482
Size: 614 3687
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1477 2356 2848 3146 1477
Size: 879 492 298 2632
Fragments arranged by size:
2632 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 926 4005 926
Size: 3079 1222
BsaAI yAC'GTr
Cuts at: 1654 1654
Size: 4301
BsaBI GATnn'nnATC
Cuts at: 6 1815 3852 6
Size: 1809 2037 455
Fragments arranged by size:
2037 1809 455
BsaHI Gr'CG_yC
Cuts at: 125 1732 1980 2682 4178 125
Size: 1607 248 702 1496 248
Fragments arranged by size:
1607 1496 702 248 248
BsaWI w'CCGG_w
Cuts at: 370 1029 1490 2011 2689 2963 3110 3521 370
Size: 659 461 521 678 274 147 411 1150
Fragments arranged by size:
1150 678 659 521 461 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 449 479 449
Size: 30 4271
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 298 391 457 298
Size: 93 66 4142
Fragments arranged by size:
4142 93 66
BseYI C'CCAG_C
Cuts at: 156 182 1001 1726 2484 3061 3557 156
Size: 26 819 725 758 577 496 900
Fragments arranged by size:
900 819 758 725 577 496 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 343 588 300
Size: 43 245 4013
Fragments arranged by size:
4013 245 43
BsiEI CG_ry'CG
Cuts at: 104 1843 1889 3097 104
Size: 1739 46 1208 1308
Fragments arranged by size:
1739 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 227 538 1029 1214 1315 2093 2283 3075 227
Size: 311 491 185 101 778 190 792 1453
Fragments arranged by size:
1453 792 778 491 311 190 185 101
Bsp1286I G_dGCh'C
Cuts at: 227 401 538 680 1029 1214 1274 1315 1926
Size: 174 137 142 349 185 60 41 611
Cuts at: 1926 2019 2093 2283 2345 3075 227
Size: 93 74 190 62 730 1453
Fragments arranged by size:
1453 730 611 349 190 185 174 142
137 93 74 62 60 41
BspEI T'CCGG_A
Cuts at: 1029 3521 1029
Size: 2492 1809
BspHI T'CATG_A
Cuts at: 3477 3477
Size: 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 284 365 616 827 1351 1565 1867 2248 284
Size: 81 251 211 524 214 302 381 2337
Fragments arranged by size:
2337 524 381 302 251 214 211 81
BsrBI CCG'CTC
Cuts at: 50 2593 2647 4131 4296 50
Size: 2543 54 1484 165 55
Fragments arranged by size:
2543 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 225 470 2213 225
Size: 245 1743 2313
Fragments arranged by size:
2313 1743 245
BsrFI r'CCGG_y
Cuts at: 370 632 2299 2480 2689 370
Size: 262 1667 181 209 1982
Fragments arranged by size:
1982 1667 262 209 181
BssSI C'ACGA_G
Cuts at: 2572 2930 2572
Size: 358 3943
BstAPI GCAn_nnn'nTGC
Cuts at: 1678 1678
Size: 4301
BstBI TT'CG_AA
Cuts at: 2662 2662
Size: 4301
BstXI CCAn_nnnn'nTGG
Cuts at: 654 786 1249 654
Size: 132 463 3706
Fragments arranged by size:
3706 463 132
BstYI r'GATC_y
Cuts at: 1 1326 1793 1816 2151 2397 3409 3495 3507
Size: 1325 467 23 335 246 1012 86 12
Cuts at: 3507 3853 1
Size: 346 449
Fragments arranged by size:
1325 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 212 212
Size: 4301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 432 2251 2432 2595 432
Size: 1819 181 163 2138
Fragments arranged by size:
2138 1819 181 163
BtsI GCAGTG_nn'
Cuts at: 690 1544 690
Size: 854 3447
DraI TTT'AAA
Cuts at: 1262 1300 1262
Size: 38 4263
DrdI GACnn_nn'nnGTC
Cuts at: 2007 2865 2007
Size: 858 3443
EaeI y'GGCC_r
Cuts at: 242 554 788 1886 2060 2451 2478 3823 242
Size: 312 234 1098 174 391 27 1345 720
Fragments arranged by size:
1345 1098 720 391 312 234 174 27
EagI C'GGCC_G
Cuts at: 1886 1886
Size: 4301
EarI CTCTTCn'nnn_
Cuts at: 1017 2324 2534 1017
Size: 1307 210 2784
Fragments arranged by size:
2784 1307 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1228 2509 2819 2965 4111 1228
Size: 1281 310 146 1146 1418
Fragments arranged by size:
1418 1281 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1027 1313 1027
Size: 286 4015
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 965 2126 2558 3305 3573 310
Size: 655 1161 432 747 268 1038
Fragments arranged by size:
1161 1038 747 655 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 384 384
Size: 4301
EcoO109I rG'GnC_Cy
Cuts at: 853 1270 1271 1440 853
Size: 417 1 169 3714
Fragments arranged by size:
3714 417 169 1
EcoRI G'AATT_C
Cuts at: 1305 1305
Size: 4301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 4301
FseI GG_CCGG'CC
Cuts at: 636 636
Size: 4301
FspI TGC'GCA
Cuts at: 2082 2082
Size: 4301
HaeII r_GCGC'y
Cuts at: 1735 1983 3005 3984 1735
Size: 248 1022 979 2052
Fragments arranged by size:
2052 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 449 481 923 955 1024 1056 2143 2173 2175
Size: 32 442 32 69 32 1087 30 2
Cuts at: 2175 2205 449
Size: 30 2545
Fragments arranged by size:
2545 1087 442 69 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1340 1340
Size: 4301
HindIII A'AGCT_T
Cuts at: 484 484
Size: 4301
KasI G'GCGC_C
Cuts at: 1731 1979 1731
Size: 248 4053
KpnI G_GTAC'C
Cuts at: 595 1321 595
Size: 726 3575
MluI A'CGCG_T
Cuts at: 3734 3734
Size: 4301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 803 2972 3156 803
Size: 2169 184 1948
Fragments arranged by size:
2169 1948 184
MscI TGG'CCA
Cuts at: 244 556 790 2062 244
Size: 312 234 1272 2483
Fragments arranged by size:
2483 1272 312 234
MslI CAynn'nnrTG
Cuts at: 652 2290 2417 652
Size: 1638 127 2536
Fragments arranged by size:
2536 1638 127
MspA1I CmG'CkG
Cuts at: 1412 1726 2086 3099 3344 1412
Size: 314 360 1013 245 2369
Fragments arranged by size:
2369 1013 360 314 245
NaeI GCC'GGC
Cuts at: 634 2482 634
Size: 1848 2453
NarI GG'CG_CC
Cuts at: 1732 1980 1732
Size: 248 4053
NdeI CA'TA_TG
Cuts at: 1248 1248
Size: 4301
NgoMIV G'CCGG_C
Cuts at: 632 2480 632
Size: 1848 2453
NspI r_CATG'y
Cuts at: 1167 1354 1854 2761 1167
Size: 187 500 907 2707
Fragments arranged by size:
2707 907 500 187
PciI A'CATG_T
Cuts at: 2757 2757
Size: 4301
PflMI CCAn_nnn'nTGG
Cuts at: 828 906 828
Size: 78 4223
PfoI T'CCnGG_A
Cuts at: 821 821
Size: 4301
PmeI GTTT'AAAC
Cuts at: 1300 1300
Size: 4301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 4269
PpuMI rG'GwC_Cy
Cuts at: 853 853
Size: 4301
PspOMI G'GGCC_C
Cuts at: 1270 1270
Size: 4301
PstI C_TGCA'G
Cuts at: 388 613 1348 2033 388
Size: 225 735 685 2656
Fragments arranged by size:
2656 735 685 225
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4301
PvuII CAG'CTG
Cuts at: 1726 2086 1726
Size: 360 3941
RsrII CG'GwC_CG
Cuts at: 2496 2496
Size: 4301
SacI G_AGCT'C
Cuts at: 1029 1315 1029
Size: 286 4015
SalI G'TCGA_C
Cuts at: 1338 1338
Size: 4301
SapI GCTCTTCn'nnn_
Cuts at: 1017 2324 2534 1017
Size: 1307 210 2784
Fragments arranged by size:
2784 1307 210
SbfI CC_TGCA'GG
Cuts at: 1348 1348
Size: 4301
ScaI AGT'ACT
Cuts at: 3818 3818
Size: 4301
SfcI C'TryA_G
Cuts at: 33 384 596 609 1344 2029 3022 3213 33
Size: 351 212 13 735 685 993 191 1121
Fragments arranged by size:
1121 993 735 685 351 212 191 13
SfoI GGC'GCC
Cuts at: 1733 1981 1733
Size: 248 4053
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
SgrAI Cr'CCGG_yG
Cuts at: 370 370
Size: 4301
SmaI CCC'GGG
Cuts at: 1153 1323 1153
Size: 170 4131
SmlI C'TyrA_G
Cuts at: 1456 2371 2863 3125 1456
Size: 915 492 262 2632
Fragments arranged by size:
2632 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4301
SphI G_CATG'C
Cuts at: 1354 1854 1354
Size: 500 3801
StyI C'CwwG_G
Cuts at: 239 509 1435 1789 239
Size: 270 926 354 2751
Fragments arranged by size:
2751 926 354 270
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 913 1884 2017 2476 2685 913
Size: 971 133 459 209 2529
Fragments arranged by size:
2529 971 459 209 133
TatI w'GTAC_w
Cuts at: 3816 3816
Size: 4301
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3818 3850 3818
Size: 32 4269
Tth111I GACn'n_nGTC
Cuts at: 2098 2098
Size: 4301
XbaI T'CTAG_A
Cuts at: 1332 1332
Size: 4301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 294 651 903 1796 294
Size: 357 252 893 2799
Fragments arranged by size:
2799 893 357 252
XmaI C'CCGG_G
Cuts at: 1151 1321 1151
Size: 170 4131
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FspAI HpaI MfeI
NcoI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI