Restriction map for
pF1KB8823
(Circular) (Six-base) MAPSORT of: pF1KB8823.seq Check: 2531 from: 1 to: 4325
pF1KB8823 4325 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1004 1004
Size: 4325
AccI GT'mk_AC
Cuts at: 554 1363 554
Size: 809 3516
Acc65I G'GTAC_C
Cuts at: 1341 1341
Size: 4325
AclI AA'CG_TT
Cuts at: 4165 4165
Size: 4325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2150 2582 3329 3597 2150
Size: 432 747 268 2878
Fragments arranged by size:
2878 747 432 268
AflIII A'CryG_T
Cuts at: 515 1677 2781 3758 515
Size: 1162 1104 977 1082
Fragments arranged by size:
1162 1104 1082 977
AgeI A'CCGG_T
Cuts at: 2713 2713
Size: 4325
AleI CACnn'nnGTG
Cuts at: 157 157
Size: 4325
AlwNI CAG_nnn'CTG
Cuts at: 746 1630 3197 746
Size: 884 1567 1874
Fragments arranged by size:
1874 1567 884
ApaI G_GGCC'C
Cuts at: 700 753 700
Size: 53 4272
ApaLI G'TGCA_C
Cuts at: 3095 3095
Size: 4325
ApoI r'AATT_y
Cuts at: 1329 3678 3934 1329
Size: 2349 256 1720
Fragments arranged by size:
2349 1720 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4325
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
AvaI C'yCGr_G
Cuts at: 1345 1345
Size: 4325
BamHI G'GATC_C
Cuts at: 1350 1350
Size: 4325
BanI G'GyrC_C
Cuts at: 723 869 1341 1755 2003 2038 723
Size: 146 472 414 248 35 3010
Fragments arranged by size:
3010 472 414 248 146 35
BanII G_rGCy'C
Cuts at: 248 700 707 753 1339 2369 248
Size: 452 7 46 586 1030 2204
Fragments arranged by size:
2204 1030 586 452 46 7
BbeI G_GCGC'C
Cuts at: 873 1759 2007 873
Size: 886 248 3191
Fragments arranged by size:
3191 886 248
BbsI GAAGACnn'nnnn_
Cuts at: 807 807
Size: 4325
BciVI GTATCCnnnnn_n'
Cuts at: 2223 2984 4325 2223
Size: 761 1341 2223
Fragments arranged by size:
2223 1341 761
BclI T'GATC_A
Cuts at: 1845 1845
Size: 4325
BglI GCCn_nnn'nGGC
Cuts at: 898 898
Size: 4325
BglII A'GATC_T
Cuts at: 1840 3877 1840
Size: 2037 2288
BlpI GC'TnA_GC
Cuts at: 247 1437 247
Size: 1190 3135
Bme1580I G_kGCm'C
Cuts at: 700 753 1150 1950 2043 3099 700
Size: 53 397 800 93 1056 1926
Fragments arranged by size:
1926 1056 800 397 93 53
BmrI ACTGGGnnnn_n'
Cuts at: 1537 1618 1953 4097 1537
Size: 81 335 2144 1765
Fragments arranged by size:
2144 1765 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 460
Size: 4325
Bpu10I CC'TnA_GC
Cuts at: 351 701 1238 1249 351
Size: 350 537 11 3427
Fragments arranged by size:
3427 537 350 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 2380 2872 3170 1501
Size: 879 492 298 2656
Fragments arranged by size:
2656 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4029 4029
Size: 4325
BsaAI yAC'GTr
Cuts at: 240 897 1678 240
Size: 657 781 2887
Fragments arranged by size:
2887 781 657
BsaBI GATnn'nnATC
Cuts at: 6 1839 3876 6
Size: 1833 2037 455
Fragments arranged by size:
2037 1833 455
BsaHI Gr'CG_yC
Cuts at: 870 1756 2004 2706 4202 870
Size: 886 248 702 1496 993
Fragments arranged by size:
1496 993 886 702 248
BsaWI w'CCGG_w
Cuts at: 1514 2035 2713 2987 3134 3545 1514
Size: 521 678 274 147 411 2294
Fragments arranged by size:
2294 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1015 1045 1015
Size: 30 4295
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1149 1149
Size: 4325
BseYI C'CCAG_C
Cuts at: 121 125 1178 1315 1750 2508 3085 3581 121
Size: 4 1053 137 435 758 577 496 865
Fragments arranged by size:
1053 865 758 577 496 435 137 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1024 650
Size: 374 3951
BsiEI CG_ry'CG
Cuts at: 104 558 1867 1913 3121 104
Size: 454 1309 46 1208 1308
Fragments arranged by size:
1309 1308 1208 454 46
BsiHKAI G_wGCw'C
Cuts at: 248 259 1339 2117 2307 3099 248
Size: 11 1080 778 190 792 1474
Fragments arranged by size:
1474 1080 792 778 190 11
BsmI GAATG_Cn'
Cuts at: 1110 1182 1110
Size: 72 4253
Bsp1286I G_dGCh'C
Cuts at: 248 259 700 707 753 1150 1339 1950 2043
Size: 11 441 7 46 397 189 611 93
Cuts at: 2043 2117 2307 2369 3099 248
Size: 74 190 62 730 1474
Fragments arranged by size:
1474 730 611 441 397 190 189 93
74 62 46 11 7
BspEI T'CCGG_A
Cuts at: 3545 3545
Size: 4325
BspHI T'CATG_A
Cuts at: 3501 3501
Size: 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1004 1375 1589 1891 2272 1004
Size: 371 214 302 381 3057
Fragments arranged by size:
3057 381 371 302 214
BsrBI CCG'CTC
Cuts at: 50 2617 2671 4155 4320 50
Size: 2567 54 1484 165 55
Fragments arranged by size:
2567 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2237 2237
Size: 4325
BsrFI r'CCGG_y
Cuts at: 836 866 1094 2323 2504 2713 836
Size: 30 228 1229 181 209 2448
Fragments arranged by size:
2448 1229 228 209 181 30
BssSI C'ACGA_G
Cuts at: 2596 2954 2596
Size: 358 3967
BstAPI GCAn_nnn'nTGC
Cuts at: 612 1702 612
Size: 1090 3235
BstBI TT'CG_AA
Cuts at: 2686 2686
Size: 4325
BstEII G'GTnAC_C
Cuts at: 150 150
Size: 4325
BstXI CCAn_nnnn'nTGG
Cuts at: 267 300 780 267
Size: 33 480 3812
Fragments arranged by size:
3812 480 33
BstYI r'GATC_y
Cuts at: 1 182 1350 1817 1840 2175 2421 3433 3519
Size: 181 1168 467 23 335 246 1012 86
Cuts at: 3519 3531 3877 1
Size: 12 346 449
Fragments arranged by size:
1168 1012 467 449 346 335 246 181
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 668 1127 668
Size: 459 3866
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 381 2275 2456 2619 381
Size: 1894 181 163 2087
Fragments arranged by size:
2087 1894 181 163
BtsI GCAGTG_nn'
Cuts at: 164 873 952 978 1568 164
Size: 709 79 26 590 2921
Fragments arranged by size:
2921 709 590 79 26
DraI TTT'AAA
Cuts at: 1324 1324
Size: 4325
DraIII CAC_nnn'GTG
Cuts at: 612 612
Size: 4325
DrdI GACnn_nn'nnGTC
Cuts at: 562 2031 2889 562
Size: 1469 858 1998
Fragments arranged by size:
1998 1469 858
EaeI y'GGCC_r
Cuts at: 290 392 650 899 1910 2084 2475 2502 3847
Size: 102 258 249 1011 174 391 27 1345
Cuts at: 3847 290
Size: 768
Fragments arranged by size:
1345 1011 768 391 258 249 174 102
27
EagI C'GGCC_G
Cuts at: 1910 1910
Size: 4325
EarI CTCTTCn'nnn_
Cuts at: 217 721 1167 2348 2558 217
Size: 504 446 1181 210 1984
Fragments arranged by size:
1984 1181 504 446 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2533 2843 2989 4135 2533
Size: 310 146 1146 2723
Fragments arranged by size:
2723 1146 310 146
EcoICRI GAG'CTC
Cuts at: 246 1337 246
Size: 1091 3234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 2150 2582 3329 3597 460
Size: 1690 432 747 268 1188
Fragments arranged by size:
1690 1188 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1126 1126
Size: 4325
EcoO109I rG'GnC_Cy
Cuts at: 130 642 696 697 750 962 1464 130
Size: 512 54 1 53 212 502 2991
Fragments arranged by size:
2991 512 502 212 54 53 1
EcoRI G'AATT_C
Cuts at: 1329 1329
Size: 4325
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 4325
FspI TGC'GCA
Cuts at: 2106 2106
Size: 4325
HaeII r_GCGC'y
Cuts at: 581 873 1759 2007 3029 4008 581
Size: 292 886 248 1022 979 898
Fragments arranged by size:
1022 979 898 886 292 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1015 1047 2167 2197 2199 2229 1015
Size: 32 1120 30 2 30 3111
Fragments arranged by size:
3111 1120 32 30 30 2
HincII GTy'rAC
Cuts at: 339 513 555 1364 339
Size: 174 42 809 3300
Fragments arranged by size:
3300 809 174 42
KasI G'GCGC_C
Cuts at: 869 1755 2003 869
Size: 886 248 3191
Fragments arranged by size:
3191 886 248
KpnI G_GTAC'C
Cuts at: 1345 1345
Size: 4325
MluI A'CGCG_T
Cuts at: 3758 3758
Size: 4325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2996 3180 2996
Size: 184 4141
MscI TGG'CCA
Cuts at: 292 394 2086 292
Size: 102 1692 2531
Fragments arranged by size:
2531 1692 102
MslI CAynn'nnrTG
Cuts at: 112 157 298 2314 2441 112
Size: 45 141 2016 127 1996
Fragments arranged by size:
2016 1996 141 127 45
MspA1I CmG'CkG
Cuts at: 125 663 687 1436 1750 2110 3123 3368 125
Size: 538 24 749 314 360 1013 245 1082
Fragments arranged by size:
1082 1013 749 538 360 314 245 24
NaeI GCC'GGC
Cuts at: 838 868 1096 2506 838
Size: 30 228 1410 2657
Fragments arranged by size:
2657 1410 228 30
NarI GG'CG_CC
Cuts at: 870 1756 2004 870
Size: 886 248 3191
Fragments arranged by size:
3191 886 248
NgoMIV G'CCGG_C
Cuts at: 836 866 1094 2504 836
Size: 30 228 1410 2657
Fragments arranged by size:
2657 1410 228 30
NspI r_CATG'y
Cuts at: 519 1154 1378 1878 2785 519
Size: 635 224 500 907 2059
Fragments arranged by size:
2059 907 635 500 224
PciI A'CATG_T
Cuts at: 515 2781 515
Size: 2266 2059
PflMI CCAn_nnn'nTGG
Cuts at: 690 690
Size: 4325
PmeI GTTT'AAAC
Cuts at: 1324 1324
Size: 4325
PmlI CAC'GTG
Cuts at: 240 240
Size: 4325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 4293
PpuMI rG'GwC_Cy
Cuts at: 642 962 642
Size: 320 4005
PspOMI G'GGCC_C
Cuts at: 696 749 696
Size: 53 4272
PstI C_TGCA'G
Cuts at: 1224 1372 2057 1224
Size: 148 685 3492
Fragments arranged by size:
3492 685 148
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4325
PvuII CAG'CTG
Cuts at: 125 687 1750 2110 125
Size: 562 1063 360 2340
Fragments arranged by size:
2340 1063 562 360
RsrII CG'GwC_CG
Cuts at: 2520 2520
Size: 4325
SacI G_AGCT'C
Cuts at: 248 1339 248
Size: 1091 3234
SalI G'TCGA_C
Cuts at: 553 1362 553
Size: 809 3516
SanDI GG'GwC_CC
Cuts at: 962 962
Size: 4325
SapI GCTCTTCn'nnn_
Cuts at: 2348 2558 2348
Size: 210 4115
SbfI CC_TGCA'GG
Cuts at: 1372 1372
Size: 4325
ScaI AGT'ACT
Cuts at: 3842 3842
Size: 4325
SexAI A'CCwGG_T
Cuts at: 986 986
Size: 4325
SfcI C'TryA_G
Cuts at: 33 1109 1220 1368 2053 3046 3237 33
Size: 1076 111 148 685 993 191 1121
Fragments arranged by size:
1121 1076 993 685 191 148 111
SfoI GGC'GCC
Cuts at: 871 1757 2005 871
Size: 886 248 3191
Fragments arranged by size:
3191 886 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
SmaI CCC'GGG
Cuts at: 1347 1347
Size: 4325
SmlI C'TyrA_G
Cuts at: 1480 2395 2887 3149 1480
Size: 915 492 262 2656
Fragments arranged by size:
2656 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4325
SphI G_CATG'C
Cuts at: 1378 1878 1378
Size: 500 3825
StyI C'CwwG_G
Cuts at: 174 1139 1199 1459 1813 174
Size: 965 60 260 354 2686
Fragments arranged by size:
2686 965 354 260 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 211 1192 1329 1908 2041 2500 2709 211
Size: 981 137 579 133 459 209 1827
Fragments arranged by size:
1827 981 579 459 209 137 133
TatI w'GTAC_w
Cuts at: 3840 3840
Size: 4325
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3842 3874 3842
Size: 32 4293
Tth111I GACn'n_nGTC
Cuts at: 2122 2122
Size: 4325
XbaI T'CTAG_A
Cuts at: 1356 1356
Size: 4325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 402 777 1820 402
Size: 375 1043 2907
Fragments arranged by size:
2907 1043 375
XmaI C'CCGG_G
Cuts at: 1345 1345
Size: 4325
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstZ17I BtgI ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PshAI PsiI PspXI
PsrI SacII SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI