Restriction map for
pF1KB8824
(Circular) (Six-base) MAPSORT of: pF1KB8824.seq Check: 9249 from: 1 to: 4355
pF1KB8824 4355 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AccI GT'mk_AC
Cuts at: 1393 1393
Size: 4355
Acc65I G'GTAC_C
Cuts at: 1371 1371
Size: 4355
AclI AA'CG_TT
Cuts at: 4195 4195
Size: 4355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 402 1069 2180 2612 3359 3627 402
Size: 667 1111 432 747 268 1130
Fragments arranged by size:
1130 1111 747 667 432 268
AflIII A'CryG_T
Cuts at: 390 1707 2811 3788 390
Size: 1317 1104 977 957
Fragments arranged by size:
1317 1104 977 957
AgeI A'CCGG_T
Cuts at: 2743 2743
Size: 4355
AleI CACnn'nnGTG
Cuts at: 127 340 127
Size: 213 4142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 421 421
Size: 4355
AlwNI CAG_nnn'CTG
Cuts at: 951 1047 1225 1660 3227 951
Size: 96 178 435 1567 2079
Fragments arranged by size:
2079 1567 435 178 96
ApaI G_GGCC'C
Cuts at: 987 987
Size: 4355
ApaLI G'TGCA_C
Cuts at: 3125 3125
Size: 4355
ApoI r'AATT_y
Cuts at: 1359 3708 3964 1359
Size: 2349 256 1750
Fragments arranged by size:
2349 1750 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4355
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
AvaI C'yCGr_G
Cuts at: 1375 1375
Size: 4355
AvrII C'CTAG_G
Cuts at: 321 321
Size: 4355
BamHI G'GATC_C
Cuts at: 1380 1380
Size: 4355
BanI G'GyrC_C
Cuts at: 288 487 602 915 924 1371 1785 2033 2068
Size: 199 115 313 9 447 414 248 35
Cuts at: 2068 288
Size: 2575
Fragments arranged by size:
2575 447 414 313 248 199 115 35
9
BanII G_rGCy'C
Cuts at: 456 581 703 987 1250 1369 2399 456
Size: 125 122 284 263 119 1030 2412
Fragments arranged by size:
2412 1030 284 263 125 122 119
BbeI G_GCGC'C
Cuts at: 1789 2037 1789
Size: 248 4107
BbsI GAAGACnn'nnnn_
Cuts at: 606 850 606
Size: 244 4111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4321
BciVI GTATCCnnnnn_n'
Cuts at: 2253 3014 4355 2253
Size: 761 1341 2253
Fragments arranged by size:
2253 1341 761
BclI T'GATC_A
Cuts at: 1875 1875
Size: 4355
BglI GCCn_nnn'nGGC
Cuts at: 241 241
Size: 4355
BglII A'GATC_T
Cuts at: 1870 3907 1870
Size: 2037 2318
BlpI GC'TnA_GC
Cuts at: 1467 1467
Size: 4355
Bme1580I G_kGCm'C
Cuts at: 605 737 920 929 987 1980 2073 3129 605
Size: 132 183 9 58 993 93 1056 1831
Fragments arranged by size:
1831 1056 993 183 132 93 58 9
BmrI ACTGGGnnnn_n'
Cuts at: 961 1567 1648 1983 4127 961
Size: 606 81 335 2144 1189
Fragments arranged by size:
2144 1189 606 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1251 2722 1251
Size: 1471 2884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 1167 139
Size: 1028 3327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 741 1531 2410 2902 3200 709
Size: 32 790 879 492 298 1864
Fragments arranged by size:
1864 879 790 492 298 32
BsaI GGTCTCn'nnnn_
Cuts at: 4059 4059
Size: 4355
BsaAI yAC'GTr
Cuts at: 391 1708 391
Size: 1317 3038
BsaBI GATnn'nnATC
Cuts at: 6 1869 3906 6
Size: 1863 2037 455
Fragments arranged by size:
2037 1863 455
BsaHI Gr'CG_yC
Cuts at: 1786 2034 2736 4232 1786
Size: 248 702 1496 1909
Fragments arranged by size:
1909 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1544 2065 2743 3017 3164 3575 1544
Size: 521 678 274 147 411 2324
Fragments arranged by size:
2324 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1140 1170 1140
Size: 30 4325
BseYI C'CCAG_C
Cuts at: 1115 1179 1206 1336 1780 2538 3115 3611 1115
Size: 64 27 130 444 758 577 496 1859
Fragments arranged by size:
1859 758 577 496 444 130 64 27
BsiEI CG_ry'CG
Cuts at: 104 1897 1943 3151 104
Size: 1793 46 1208 1308
Fragments arranged by size:
1793 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 134 1250 1369 2147 2337 3129 134
Size: 1116 119 778 190 792 1360
Fragments arranged by size:
1360 1116 792 778 190 119
Bsp1286I G_dGCh'C
Cuts at: 134 456 581 605 703 737 920 929 987
Size: 322 125 24 98 34 183 9 58
Cuts at: 987 1250 1369 1980 2073 2147 2337 2399 3129
Size: 263 119 611 93 74 190 62 730
Cuts at: 3129 134
Size: 1360
Fragments arranged by size:
1360 730 611 322 263 190 183 125
119 98 93 74 62 58 34 24
9
BspEI T'CCGG_A
Cuts at: 3575 3575
Size: 4355
BspHI T'CATG_A
Cuts at: 3531 3531
Size: 4355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 701 1274 1405 1619 1921 2302 701
Size: 573 131 214 302 381 2754
Fragments arranged by size:
2754 573 381 302 214 131
BsrBI CCG'CTC
Cuts at: 50 2647 2701 4185 4350 50
Size: 2597 54 1484 165 55
Fragments arranged by size:
2597 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2267 2267
Size: 4355
BsrFI r'CCGG_y
Cuts at: 905 2353 2534 2743 905
Size: 1448 181 209 2517
Fragments arranged by size:
2517 1448 209 181
BssSI C'ACGA_G
Cuts at: 2626 2984 2626
Size: 358 3997
BstAPI GCAn_nnn'nTGC
Cuts at: 1158 1732 1158
Size: 574 3781
BstBI TT'CG_AA
Cuts at: 2716 2716
Size: 4355
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4355
BstYI r'GATC_y
Cuts at: 1 1221 1380 1847 1870 2205 2451 3463 3549
Size: 1220 159 467 23 335 246 1012 86
Cuts at: 3549 3561 3907 1
Size: 12 346 449
Fragments arranged by size:
1220 1012 467 449 346 335 246 159
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 806 806
Size: 4355
BtgI C'CryG_G
Cuts at: 957 1086 957
Size: 129 4226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2305 2486 2649 2305
Size: 181 163 4011
Fragments arranged by size:
4011 181 163
BtsI GCAGTG_nn'
Cuts at: 270 676 1007 1598 270
Size: 406 331 591 3027
Fragments arranged by size:
3027 591 406 331
DraI TTT'AAA
Cuts at: 1354 1354
Size: 4355
DrdI GACnn_nn'nnGTC
Cuts at: 2061 2919 2061
Size: 858 3497
EaeI y'GGCC_r
Cuts at: 242 551 872 988 1940 2114 2505 2532 3877
Size: 309 321 116 952 174 391 27 1345
Cuts at: 3877 242
Size: 720
Fragments arranged by size:
1345 952 720 391 321 309 174 116
27
EagI C'GGCC_G
Cuts at: 1940 1940
Size: 4355
EarI CTCTTCn'nnn_
Cuts at: 278 2378 2588 278
Size: 2100 210 2045
Fragments arranged by size:
2100 2045 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2563 2873 3019 4165 2563
Size: 310 146 1146 2753
Fragments arranged by size:
2753 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1248 1367 1248
Size: 119 4236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 402 1069 1167 2180 2612 3359 3627 139
Size: 263 667 98 1013 432 747 268 867
Fragments arranged by size:
1013 867 747 667 432 268 263 98
EcoO109I rG'GnC_Cy
Cuts at: 197 983 1494 197
Size: 786 511 3058
Fragments arranged by size:
3058 786 511
EcoRI G'AATT_C
Cuts at: 1359 1359
Size: 4355
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4355
FspI TGC'GCA
Cuts at: 2136 2136
Size: 4355
HaeII r_GCGC'y
Cuts at: 1203 1789 2037 3059 4038 1203
Size: 586 248 1022 979 1520
Fragments arranged by size:
1520 1022 979 586 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 730 762 953 985 2197 2227 2229 2259 730
Size: 32 191 32 1212 30 2 30 2826
Fragments arranged by size:
2826 1212 191 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1059 1394 1059
Size: 335 4020
KasI G'GCGC_C
Cuts at: 1785 2033 1785
Size: 248 4107
KpnI G_GTAC'C
Cuts at: 1375 1375
Size: 4355
MluI A'CGCG_T
Cuts at: 3788 3788
Size: 4355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3026 3210 3026
Size: 184 4171
MscI TGG'CCA
Cuts at: 553 874 2116 553
Size: 321 1242 2792
Fragments arranged by size:
2792 1242 321
MslI CAynn'nnrTG
Cuts at: 127 340 427 608 1072 2344 2471 127
Size: 213 87 181 464 1272 127 2011
Fragments arranged by size:
2011 1272 464 213 181 127 87
MspA1I CmG'CkG
Cuts at: 295 650 1119 1466 1780 2140 3153 3398 295
Size: 355 469 347 314 360 1013 245 1252
Fragments arranged by size:
1252 1013 469 360 355 347 314 245
NaeI GCC'GGC
Cuts at: 907 2536 907
Size: 1629 2726
NarI GG'CG_CC
Cuts at: 1786 2034 1786
Size: 248 4107
NcoI C'CATG_G
Cuts at: 957 957
Size: 4355
NgoMIV G'CCGG_C
Cuts at: 905 2534 905
Size: 1629 2726
NspI r_CATG'y
Cuts at: 408 432 1000 1408 1908 2815 408
Size: 24 568 408 500 907 1948
Fragments arranged by size:
1948 907 568 500 408 24
PasI CC'CwG_GG
Cuts at: 911 911
Size: 4355
PciI A'CATG_T
Cuts at: 2811 2811
Size: 4355
PflMI CCAn_nnn'nTGG
Cuts at: 626 626
Size: 4355
PfoI T'CCnGG_A
Cuts at: 560 857 560
Size: 297 4058
PmeI GTTT'AAAC
Cuts at: 1354 1354
Size: 4355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 4323
PpuMI rG'GwC_Cy
Cuts at: 197 197
Size: 4355
PspOMI G'GGCC_C
Cuts at: 983 983
Size: 4355
PstI C_TGCA'G
Cuts at: 698 1402 2087 698
Size: 704 685 2966
Fragments arranged by size:
2966 704 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4355
PvuII CAG'CTG
Cuts at: 295 1119 1780 2140 295
Size: 824 661 360 2510
Fragments arranged by size:
2510 824 661 360
RsrII CG'GwC_CG
Cuts at: 2550 2550
Size: 4355
SacI G_AGCT'C
Cuts at: 1250 1369 1250
Size: 119 4236
SalI G'TCGA_C
Cuts at: 1392 1392
Size: 4355
SapI GCTCTTCn'nnn_
Cuts at: 2378 2588 2378
Size: 210 4145
SbfI CC_TGCA'GG
Cuts at: 1402 1402
Size: 4355
ScaI AGT'ACT
Cuts at: 3872 3872
Size: 4355
SfcI C'TryA_G
Cuts at: 33 296 694 1398 2083 3076 3267 33
Size: 263 398 704 685 993 191 1121
Fragments arranged by size:
1121 993 704 685 398 263 191
SfoI GGC'GCC
Cuts at: 1787 2035 1787
Size: 248 4107
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
SmaI CCC'GGG
Cuts at: 1377 1377
Size: 4355
SmlI C'TyrA_G
Cuts at: 724 756 1510 2425 2917 3179 724
Size: 32 754 915 492 262 1900
Fragments arranged by size:
1900 915 754 492 262 32
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4355
SphI G_CATG'C
Cuts at: 1000 1408 1908 1000
Size: 408 500 3447
Fragments arranged by size:
3447 500 408
StuI AGG'CCT
Cuts at: 160 473 160
Size: 313 4042
StyI C'CwwG_G
Cuts at: 321 769 785 957 1489 1843 321
Size: 448 16 172 532 354 2833
Fragments arranged by size:
2833 532 448 354 172 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 903 1129 1171 1938 2071 2530 2739 903
Size: 226 42 767 133 459 209 2519
Fragments arranged by size:
2519 767 459 226 209 133 42
TatI w'GTAC_w
Cuts at: 476 3870 476
Size: 3394 961
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 627 659 1140 1172 3872 3904 627
Size: 32 481 32 2700 32 1078
Fragments arranged by size:
2700 1078 481 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2152 2152
Size: 4355
XbaI T'CTAG_A
Cuts at: 1386 1386
Size: 4355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 299 1850 299
Size: 1551 2804
XmaI C'CCGG_G
Cuts at: 1375 1375
Size: 4355
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI BaeI
BbvCI BfrBI BmgBI BmtI Bpu10I BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI