Restriction map for
pF1KB8825
(Circular) (Six-base) MAPSORT of: pF1KB8825.seq Check: 6751 from: 1 to: 4358
pF1KB8825 4358 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 619 769 619
Size: 150 4208
AccI GT'mk_AC
Cuts at: 1136 1396 1136
Size: 260 4098
Acc65I G'GTAC_C
Cuts at: 1374 1374
Size: 4358
AclI AA'CG_TT
Cuts at: 4198 4198
Size: 4358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 372 2183 2615 3362 3630 300
Size: 72 1811 432 747 268 1028
Fragments arranged by size:
1811 1028 747 432 268 72
AflIII A'CryG_T
Cuts at: 1710 2814 3791 1710
Size: 1104 977 2277
Fragments arranged by size:
2277 1104 977
AgeI A'CCGG_T
Cuts at: 2746 2746
Size: 4358
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 907 907
Size: 4358
AlwNI CAG_nnn'CTG
Cuts at: 1663 3230 1663
Size: 1567 2791
ApaI G_GGCC'C
Cuts at: 740 740
Size: 4358
ApaLI G'TGCA_C
Cuts at: 3128 3128
Size: 4358
ApoI r'AATT_y
Cuts at: 1362 3711 3967 1362
Size: 2349 256 1753
Fragments arranged by size:
2349 1753 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4358
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
AvaI C'yCGr_G
Cuts at: 1344 1378 1344
Size: 34 4324
BamHI G'GATC_C
Cuts at: 1383 1383
Size: 4358
BanI G'GyrC_C
Cuts at: 631 995 1374 1788 2036 2071 631
Size: 364 379 414 248 35 2918
Fragments arranged by size:
2918 414 379 364 248 35
BanII G_rGCy'C
Cuts at: 666 740 782 1280 1372 2402 666
Size: 74 42 498 92 1030 2622
Fragments arranged by size:
2622 1030 498 92 74 42
BbeI G_GCGC'C
Cuts at: 999 1792 2040 999
Size: 793 248 3317
Fragments arranged by size:
3317 793 248
BbsI GAAGACnn'nnnn_
Cuts at: 141 1289 141
Size: 1148 3210
BciVI GTATCCnnnnn_n'
Cuts at: 2256 3017 4358 2256
Size: 761 1341 2256
Fragments arranged by size:
2256 1341 761
BclI T'GATC_A
Cuts at: 1878 1878
Size: 4358
BglII A'GATC_T
Cuts at: 1873 3910 1873
Size: 2037 2321
BlpI GC'TnA_GC
Cuts at: 484 1470 484
Size: 986 3372
Bme1580I G_kGCm'C
Cuts at: 636 740 1983 2076 3132 636
Size: 104 1243 93 1056 1862
Fragments arranged by size:
1862 1243 1056 104 93
BmgBI CAC'GTC
Cuts at: 1140 1140
Size: 4358
BmrI ACTGGGnnnn_n'
Cuts at: 1570 1651 1986 4130 1570
Size: 81 335 2144 1798
Fragments arranged by size:
2144 1798 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 369 2725 369
Size: 2356 2002
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 1264 328
Size: 936 3422
Bpu10I CC'TnA_GC
Cuts at: 502 502
Size: 4358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1534 2413 2905 3203 1534
Size: 879 492 298 2689
Fragments arranged by size:
2689 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1318 4062 1318
Size: 2744 1614
BsaAI yAC'GTr
Cuts at: 1711 1711
Size: 4358
BsaBI GATnn'nnATC
Cuts at: 6 1872 3909 6
Size: 1866 2037 455
Fragments arranged by size:
2037 1866 455
BsaHI Gr'CG_yC
Cuts at: 287 996 1789 2037 2739 4235 287
Size: 709 793 248 702 1496 410
Fragments arranged by size:
1496 793 709 702 410 248
BsaWI w'CCGG_w
Cuts at: 1547 2068 2746 3020 3167 3578 1547
Size: 521 678 274 147 411 2327
Fragments arranged by size:
2327 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1219 1249 1219
Size: 30 4328
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 173 424 173
Size: 251 4107
BseYI C'CCAG_C
Cuts at: 444 453 719 1783 2541 3118 3614 444
Size: 9 266 1064 758 577 496 1188
Fragments arranged by size:
1188 1064 758 577 496 266 9
BsiEI CG_ry'CG
Cuts at: 104 405 1900 1946 3154 104
Size: 301 1495 46 1208 1308
Fragments arranged by size:
1495 1308 1208 301 46
BsiHKAI G_wGCw'C
Cuts at: 1372 2150 2340 3132 1372
Size: 778 190 792 2598
Fragments arranged by size:
2598 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 636 666 740 782 1280 1372 1983 2076 2150
Size: 30 74 42 498 92 611 93 74
Cuts at: 2150 2340 2402 3132 636
Size: 190 62 730 1862
Fragments arranged by size:
1862 730 611 498 190 93 92 74
74 62 42 30
BspEI T'CCGG_A
Cuts at: 3578 3578
Size: 4358
BspHI T'CATG_A
Cuts at: 3534 3534
Size: 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 619 769 1408 1622 1924 2305 619
Size: 150 639 214 302 381 2672
Fragments arranged by size:
2672 639 381 302 214 150
BsrBI CCG'CTC
Cuts at: 50 570 1230 2650 2704 4188 4353 50
Size: 520 660 1420 54 1484 165 55
Fragments arranged by size:
1484 1420 660 520 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2270 2270
Size: 4358
BsrFI r'CCGG_y
Cuts at: 488 2356 2537 2746 488
Size: 1868 181 209 2100
Fragments arranged by size:
2100 1868 209 181
BssSI C'ACGA_G
Cuts at: 354 2629 2987 354
Size: 2275 358 1725
Fragments arranged by size:
2275 1725 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1735 1735
Size: 4358
BstBI TT'CG_AA
Cuts at: 2719 2719
Size: 4358
BstEII G'GTnAC_C
Cuts at: 1152 1152
Size: 4358
BstXI CCAn_nnnn'nTGG
Cuts at: 1014 1014
Size: 4358
BstYI r'GATC_y
Cuts at: 1 1383 1850 1873 2208 2454 3466 3552 3564
Size: 1382 467 23 335 246 1012 86 12
Cuts at: 3564 3910 1
Size: 346 449
Fragments arranged by size:
1382 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1158 1158
Size: 4358
BtgI C'CryG_G
Cuts at: 107 107
Size: 4358
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2308 2489 2652 2308
Size: 181 163 4014
Fragments arranged by size:
4014 181 163
BtsI GCAGTG_nn'
Cuts at: 562 584 852 1176 1601 562
Size: 22 268 324 425 3319
Fragments arranged by size:
3319 425 324 268 22
DraI TTT'AAA
Cuts at: 1357 1357
Size: 4358
DraIII CAC_nnn'GTG
Cuts at: 276 1068 276
Size: 792 3566
DrdI GACnn_nn'nnGTC
Cuts at: 589 2064 2922 589
Size: 1475 858 2025
Fragments arranged by size:
2025 1475 858
EaeI y'GGCC_r
Cuts at: 965 1119 1142 1943 2117 2508 2535 3880 965
Size: 154 23 801 174 391 27 1345 1443
Fragments arranged by size:
1443 1345 801 391 174 154 27 23
EagI C'GGCC_G
Cuts at: 1943 1943
Size: 4358
EarI CTCTTCn'nnn_
Cuts at: 327 643 2381 2591 327
Size: 316 1738 210 2094
Fragments arranged by size:
2094 1738 316 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 507 2566 2876 3022 4168 507
Size: 2059 310 146 1146 697
Fragments arranged by size:
2059 1146 697 310 146
EcoICRI GAG'CTC
Cuts at: 1370 1370
Size: 4358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 328 372 1264 2183 2615 3362 3630 300
Size: 28 44 892 919 432 747 268 1028
Fragments arranged by size:
1028 919 892 747 432 268 44 28
EcoO109I rG'GnC_Cy
Cuts at: 736 737 1497 736
Size: 1 760 3597
Fragments arranged by size:
3597 760 1
EcoRI G'AATT_C
Cuts at: 1362 1362
Size: 4358
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4358
FspI TGC'GCA
Cuts at: 2139 2139
Size: 4358
HaeII r_GCGC'y
Cuts at: 840 999 1103 1792 2040 3062 4041 840
Size: 159 104 689 248 1022 979 1157
Fragments arranged by size:
1157 1022 979 689 248 159 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1044 1076 2200 2230 2232 2262 1044
Size: 32 1124 30 2 30 3140
Fragments arranged by size:
3140 1124 32 30 30 2
HincII GTy'rAC
Cuts at: 1397 1397
Size: 4358
KasI G'GCGC_C
Cuts at: 995 1788 2036 995
Size: 793 248 3317
Fragments arranged by size:
3317 793 248
KpnI G_GTAC'C
Cuts at: 1378 1378
Size: 4358
MluI A'CGCG_T
Cuts at: 3791 3791
Size: 4358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3029 3213 3029
Size: 184 4174
MscI TGG'CCA
Cuts at: 1144 2119 1144
Size: 975 3383
MslI CAynn'nnrTG
Cuts at: 1087 2347 2474 1087
Size: 1260 127 2971
Fragments arranged by size:
2971 1260 127
MspA1I CmG'CkG
Cuts at: 906 993 1194 1469 1783 2143 3156 3401 906
Size: 87 201 275 314 360 1013 245 1863
Fragments arranged by size:
1863 1013 360 314 275 245 201 87
NaeI GCC'GGC
Cuts at: 490 2539 490
Size: 2049 2309
NarI GG'CG_CC
Cuts at: 996 1789 2037 996
Size: 793 248 3317
Fragments arranged by size:
3317 793 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4358
NgoMIV G'CCGG_C
Cuts at: 488 2537 488
Size: 2049 2309
NspI r_CATG'y
Cuts at: 1176 1411 1911 2818 1176
Size: 235 500 907 2716
Fragments arranged by size:
2716 907 500 235
PciI A'CATG_T
Cuts at: 2814 2814
Size: 4358
PflMI CCAn_nnn'nTGG
Cuts at: 963 963
Size: 4358
PfoI T'CCnGG_A
Cuts at: 305 1223 305
Size: 918 3440
PmeI GTTT'AAAC
Cuts at: 1357 1357
Size: 4358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 304 336 3497 3529 304
Size: 32 3161 32 1133
Fragments arranged by size:
3161 1133 32 32
PspOMI G'GGCC_C
Cuts at: 736 736
Size: 4358
PstI C_TGCA'G
Cuts at: 905 1405 2090 905
Size: 500 685 3173
Fragments arranged by size:
3173 685 500
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4358
PvuII CAG'CTG
Cuts at: 906 1783 2143 906
Size: 877 360 3121
Fragments arranged by size:
3121 877 360
RsrII CG'GwC_CG
Cuts at: 2553 2553
Size: 4358
SacI G_AGCT'C
Cuts at: 1372 1372
Size: 4358
SalI G'TCGA_C
Cuts at: 1395 1395
Size: 4358
SapI GCTCTTCn'nnn_
Cuts at: 327 2381 2591 327
Size: 2054 210 2094
Fragments arranged by size:
2094 2054 210
SbfI CC_TGCA'GG
Cuts at: 1405 1405
Size: 4358
ScaI AGT'ACT
Cuts at: 3875 3875
Size: 4358
SexAI A'CCwGG_T
Cuts at: 1064 1064
Size: 4358
SfcI C'TryA_G
Cuts at: 33 426 536 901 1401 2086 3079 3270 33
Size: 393 110 365 500 685 993 191 1121
Fragments arranged by size:
1121 993 685 500 393 365 191 110
SfoI GGC'GCC
Cuts at: 997 1790 2038 997
Size: 793 248 3317
Fragments arranged by size:
3317 793 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
SmaI CCC'GGG
Cuts at: 1380 1380
Size: 4358
SmlI C'TyrA_G
Cuts at: 1513 2428 2920 3182 1513
Size: 915 492 262 2689
Fragments arranged by size:
2689 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4358
SphI G_CATG'C
Cuts at: 1411 1911 1411
Size: 500 3858
StuI AGG'CCT
Cuts at: 221 1163 1336 221
Size: 942 173 3243
Fragments arranged by size:
3243 942 173
StyI C'CwwG_G
Cuts at: 107 1492 1846 107
Size: 1385 354 2619
Fragments arranged by size:
2619 1385 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 733 865 1140 1941 2074 2533 2742 733
Size: 132 275 801 133 459 209 2349
Fragments arranged by size:
2349 801 459 275 209 133 132
TatI w'GTAC_w
Cuts at: 3873 3873
Size: 4358
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1219 1251 3875 3907 1219
Size: 32 2624 32 1670
Fragments arranged by size:
2624 1670 32 32
Tth111I GACn'n_nGTC
Cuts at: 2155 2155
Size: 4358
XbaI T'CTAG_A
Cuts at: 1389 1389
Size: 4358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 601 1014 1853 601
Size: 413 839 3106
Fragments arranged by size:
3106 839 413
XmaI C'CCGG_G
Cuts at: 1378 1378
Size: 4358
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI