Restriction map for
pF1KB8828
(Circular) (Six-base) MAPSORT of: pF1KB8828.seq Check: 2879 from: 1 to: 4415
pF1KB8828 4415 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 590 590
Size: 4415
AatII G_ACGT'C
Cuts at: 666 666
Size: 4415
AccI GT'mk_AC
Cuts at: 1453 1453
Size: 4415
Acc65I G'GTAC_C
Cuts at: 136 1431 136
Size: 1295 3120
AclI AA'CG_TT
Cuts at: 4255 4255
Size: 4415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2240 2672 3419 3687 2240
Size: 432 747 268 2968
Fragments arranged by size:
2968 747 432 268
AflIII A'CryG_T
Cuts at: 1407 1767 2871 3848 1407
Size: 360 1104 977 1974
Fragments arranged by size:
1974 1104 977 360
AgeI A'CCGG_T
Cuts at: 2803 2803
Size: 4415
AlwNI CAG_nnn'CTG
Cuts at: 979 990 1720 3287 979
Size: 11 730 1567 2107
Fragments arranged by size:
2107 1567 730 11
ApaLI G'TGCA_C
Cuts at: 3185 3185
Size: 4415
ApoI r'AATT_y
Cuts at: 1419 3768 4024 1419
Size: 2349 256 1810
Fragments arranged by size:
2349 1810 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4415
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
AvaI C'yCGr_G
Cuts at: 608 1435 608
Size: 827 3588
BamHI G'GATC_C
Cuts at: 1440 1440
Size: 4415
BanI G'GyrC_C
Cuts at: 136 1239 1431 1845 2093 2128 136
Size: 1103 192 414 248 35 2423
Fragments arranged by size:
2423 1103 414 248 192 35
BanII G_rGCy'C
Cuts at: 637 964 1119 1429 2459 637
Size: 327 155 310 1030 2593
Fragments arranged by size:
2593 1030 327 310 155
BbeI G_GCGC'C
Cuts at: 1849 2097 1849
Size: 248 4167
BbsI GAAGACnn'nnnn_
Cuts at: 433 433
Size: 4415
BbvCI CC'TCA_GC
Cuts at: 451 451
Size: 4415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 728 762 90
Size: 34 604 34 3743
Fragments arranged by size:
3743 604 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2313 3074 4415 2313
Size: 761 1341 2313
Fragments arranged by size:
2313 1341 761
BclI T'GATC_A
Cuts at: 1935 1935
Size: 4415
BglI GCCn_nnn'nGGC
Cuts at: 190 1297 190
Size: 1107 3308
BglII A'GATC_T
Cuts at: 1930 3967 1930
Size: 2037 2378
BlpI GC'TnA_GC
Cuts at: 1527 1527
Size: 4415
Bme1580I G_kGCm'C
Cuts at: 1009 2040 2133 3189 1009
Size: 1031 93 1056 2235
Fragments arranged by size:
2235 1056 1031 93
BmrI ACTGGGnnnn_n'
Cuts at: 1627 1708 2043 4187 1627
Size: 81 335 2144 1855
Fragments arranged by size:
2144 1855 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 4415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 1103 1133 182
Size: 921 30 3464
Fragments arranged by size:
3464 921 30
Bpu10I CC'TnA_GC
Cuts at: 451 847 958 451
Size: 396 111 3908
Fragments arranged by size:
3908 396 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 523 1591 2470 2962 3260 264
Size: 259 1068 879 492 298 1419
Fragments arranged by size:
1419 1068 879 492 298 259
BsaI GGTCTCn'nnnn_
Cuts at: 4119 4119
Size: 4415
BsaAI yAC'GTr
Cuts at: 1768 1768
Size: 4415
BsaBI GATnn'nnATC
Cuts at: 6 1929 3966 6
Size: 1923 2037 455
Fragments arranged by size:
2037 1923 455
BsaHI Gr'CG_yC
Cuts at: 663 1846 2094 2796 4292 663
Size: 1183 248 702 1496 786
Fragments arranged by size:
1496 1183 786 702 248
BsaWI w'CCGG_w
Cuts at: 1604 2125 2803 3077 3224 3635 1604
Size: 521 678 274 147 411 2384
Fragments arranged by size:
2384 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 135 165 1335 1365 135
Size: 30 1170 30 3185
Fragments arranged by size:
3185 1170 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 526 572 632 844 847 877 193
Size: 333 46 60 212 3 30 3731
Fragments arranged by size:
3731 333 212 60 46 30 3
BseYI C'CCAG_C
Cuts at: 1088 1840 2598 3175 3671 1088
Size: 752 758 577 496 1832
Fragments arranged by size:
1832 758 752 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 837 953 1127 837
Size: 116 174 4125
Fragments arranged by size:
4125 174 116
BsiEI CG_ry'CG
Cuts at: 104 1957 2003 3211 104
Size: 1853 46 1208 1308
Fragments arranged by size:
1853 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 964 1119 1429 2207 2397 3189 964
Size: 155 310 778 190 792 2190
Fragments arranged by size:
2190 792 778 310 190 155
BsmI GAATG_Cn'
Cuts at: 843 843
Size: 4415
Bsp1286I G_dGCh'C
Cuts at: 637 964 1009 1119 1429 2040 2133 2207 2397
Size: 327 45 110 310 611 93 74 190
Cuts at: 2397 2459 3189 637
Size: 62 730 1863
Fragments arranged by size:
1863 730 611 327 310 190 110 93
74 62 45
BspEI T'CCGG_A
Cuts at: 3635 3635
Size: 4415
BspHI T'CATG_A
Cuts at: 666 3591 666
Size: 2925 1490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 590 867 896 1465 1679 1981 2362 590
Size: 277 29 569 214 302 381 2643
Fragments arranged by size:
2643 569 381 302 277 214 29
BsrBI CCG'CTC
Cuts at: 50 2707 2761 4245 4410 50
Size: 2657 54 1484 165 55
Fragments arranged by size:
2657 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1031 2327 1031
Size: 1296 3119
BsrFI r'CCGG_y
Cuts at: 486 734 2413 2594 2803 486
Size: 248 1679 181 209 2098
Fragments arranged by size:
2098 1679 248 209 181
BssSI C'ACGA_G
Cuts at: 2686 3044 2686
Size: 358 4057
BstAPI GCAn_nnn'nTGC
Cuts at: 272 1150 1792 272
Size: 878 642 2895
Fragments arranged by size:
2895 878 642
BstBI TT'CG_AA
Cuts at: 2776 2776
Size: 4415
BstXI CCAn_nnnn'nTGG
Cuts at: 1356 1356
Size: 4415
BstYI r'GATC_y
Cuts at: 1 1440 1907 1930 2265 2511 3523 3609 3621
Size: 1439 467 23 335 246 1012 86 12
Cuts at: 3621 3967 1
Size: 346 449
Fragments arranged by size:
1439 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 393 445 1026 1256 1355 393
Size: 52 581 230 99 3453
Fragments arranged by size:
3453 581 230 99 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2365 2546 2709 2365
Size: 181 163 4071
Fragments arranged by size:
4071 181 163
BtsI GCAGTG_nn'
Cuts at: 210 965 1658 210
Size: 755 693 2967
Fragments arranged by size:
2967 755 693
ClaI AT'CG_AT
Cuts at: 1306 1306
Size: 4415
DraI TTT'AAA
Cuts at: 1414 1414
Size: 4415
DrdI GACnn_nn'nnGTC
Cuts at: 2121 2979 2121
Size: 858 3557
EaeI y'GGCC_r
Cuts at: 893 1397 2000 2174 2565 2592 3937 893
Size: 504 603 174 391 27 1345 1371
Fragments arranged by size:
1371 1345 603 504 391 174 27
EagI C'GGCC_G
Cuts at: 2000 2000
Size: 4415
EarI CTCTTCn'nnn_
Cuts at: 2438 2648 2438
Size: 210 4205
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2623 2933 3079 4225 2623
Size: 310 146 1146 2813
Fragments arranged by size:
2813 1146 310 146
EcoICRI GAG'CTC
Cuts at: 962 1117 1427 962
Size: 155 310 3950
Fragments arranged by size:
3950 310 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 1103 1133 2240 2672 3419 3687 182
Size: 921 30 1107 432 747 268 910
Fragments arranged by size:
1107 921 910 747 432 268 30
EcoO109I rG'GnC_Cy
Cuts at: 157 516 1554 157
Size: 359 1038 3018
Fragments arranged by size:
3018 1038 359
EcoRI G'AATT_C
Cuts at: 1419 1419
Size: 4415
EcoRV GAT'ATC
Cuts at: 280 681 280
Size: 401 4014
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4415
FspI TGC'GCA
Cuts at: 1390 2196 1390
Size: 806 3609
FspAI rTGC'GCAy
Cuts at: 1390 1390
Size: 4415
HaeII r_GCGC'y
Cuts at: 1849 2097 3119 4098 1849
Size: 248 1022 979 2166
Fragments arranged by size:
2166 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2257 2287 2289 2319 2257
Size: 30 2 30 4353
Fragments arranged by size:
4353 30 30 2
HincII GTy'rAC
Cuts at: 1454 1454
Size: 4415
KasI G'GCGC_C
Cuts at: 1845 2093 1845
Size: 248 4167
KpnI G_GTAC'C
Cuts at: 140 1435 140
Size: 1295 3120
MfeI C'AATT_G
Cuts at: 1223 1223
Size: 4415
MluI A'CGCG_T
Cuts at: 3848 3848
Size: 4415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 332 3086 3270 332
Size: 2754 184 1477
Fragments arranged by size:
2754 1477 184
MscI TGG'CCA
Cuts at: 895 2176 895
Size: 1281 3134
MslI CAynn'nnrTG
Cuts at: 1354 1399 2404 2531 1354
Size: 45 1005 127 3238
Fragments arranged by size:
3238 1005 127 45
MspA1I CmG'CkG
Cuts at: 149 469 1123 1207 1526 1840 2200 3213 3458
Size: 320 654 84 319 314 360 1013 245
Cuts at: 3458 149
Size: 1106
Fragments arranged by size:
1106 1013 654 360 320 319 314 245
84
NaeI GCC'GGC
Cuts at: 736 2596 736
Size: 1860 2555
NarI GG'CG_CC
Cuts at: 1846 2094 1846
Size: 248 4167
NcoI C'CATG_G
Cuts at: 393 1026 1256 1355 393
Size: 633 230 99 3453
Fragments arranged by size:
3453 633 230 99
NdeI CA'TA_TG
Cuts at: 780 780
Size: 4415
NgoMIV G'CCGG_C
Cuts at: 734 2594 734
Size: 1860 2555
NspI r_CATG'y
Cuts at: 1389 1468 1968 2875 1389
Size: 79 500 907 2929
Fragments arranged by size:
2929 907 500 79
PasI CC'CwG_GG
Cuts at: 1230 1230
Size: 4415
PciI A'CATG_T
Cuts at: 2871 2871
Size: 4415
PflMI CCAn_nnn'nTGG
Cuts at: 1236 1320 1236
Size: 84 4331
PmeI GTTT'AAAC
Cuts at: 1414 1414
Size: 4415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4383
PpuMI rG'GwC_Cy
Cuts at: 516 516
Size: 4415
PstI C_TGCA'G
Cuts at: 1462 2147 1462
Size: 685 3730
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4415
PvuII CAG'CTG
Cuts at: 469 1123 1207 1840 2200 469
Size: 654 84 633 360 2684
Fragments arranged by size:
2684 654 633 360 84
RsrII CG'GwC_CG
Cuts at: 2610 2610
Size: 4415
SacI G_AGCT'C
Cuts at: 964 1119 1429 964
Size: 155 310 3950
Fragments arranged by size:
3950 310 155
SalI G'TCGA_C
Cuts at: 1452 1452
Size: 4415
SapI GCTCTTCn'nnn_
Cuts at: 2438 2648 2438
Size: 210 4205
SbfI CC_TGCA'GG
Cuts at: 1462 1462
Size: 4415
ScaI AGT'ACT
Cuts at: 3932 3932
Size: 4415
SfcI C'TryA_G
Cuts at: 33 231 1069 1458 2143 3136 3327 33
Size: 198 838 389 685 993 191 1121
Fragments arranged by size:
1121 993 838 685 389 198 191
SfoI GGC'GCC
Cuts at: 1847 2095 1847
Size: 248 4167
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
SgrAI Cr'CCGG_yG
Cuts at: 734 734
Size: 4415
SmaI CCC'GGG
Cuts at: 1437 1437
Size: 4415
SmlI C'TyrA_G
Cuts at: 243 538 1570 2485 2977 3239 243
Size: 295 1032 915 492 262 1419
Fragments arranged by size:
1419 1032 915 492 295 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4415
SphI G_CATG'C
Cuts at: 1389 1468 1968 1389
Size: 79 500 3836
Fragments arranged by size:
3836 500 79
StyI C'CwwG_G
Cuts at: 393 401 474 944 1026 1256 1319 1355 1549
Size: 8 73 470 82 230 63 36 194
Cuts at: 1549 1903 393
Size: 354 2905
Fragments arranged by size:
2905 470 354 230 194 82 73 63
36 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 302 1998 2131 2590 2799 302
Size: 1696 133 459 209 1918
Fragments arranged by size:
1918 1696 459 209 133
TatI w'GTAC_w
Cuts at: 3930 3930
Size: 4415
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 135 167 1335 1367 3932 3964 135
Size: 32 1168 32 2565 32 586
Fragments arranged by size:
2565 1168 586 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 662 2212 662
Size: 1550 2865
XbaI T'CTAG_A
Cuts at: 1446 1446
Size: 4415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1263 1353 1910 1263
Size: 90 557 3768
Fragments arranged by size:
3768 557 90
XmaI C'CCGG_G
Cuts at: 1435 1435
Size: 4415
ZraI GAC'GTC
Cuts at: 664 664
Size: 4415
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoNI FseI
HindIII HpaI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI
XmnI