Restriction map for
pF1KB8843
(Circular) (Six-base) MAPSORT of: pF1KB8843.seq Check: 3269 from: 1 to: 4601
pF1KB8843 4601 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AatII G_ACGT'C
Cuts at: 975 975
Size: 4601
AccI GT'mk_AC
Cuts at: 954 1639 954
Size: 685 3916
Acc65I G'GTAC_C
Cuts at: 1617 1617
Size: 4601
AclI AA'CG_TT
Cuts at: 4441 4441
Size: 4601
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 914 1070 2426 2858 3605 3873 914
Size: 156 1356 432 747 268 1642
Fragments arranged by size:
1642 1356 747 432 268 156
AfeI AGC'GCT
Cuts at: 916 916
Size: 4601
AflIII A'CryG_T
Cuts at: 1953 3057 4034 1953
Size: 1104 977 2520
Fragments arranged by size:
2520 1104 977
AgeI A'CCGG_T
Cuts at: 949 2989 949
Size: 2040 2561
AlwNI CAG_nnn'CTG
Cuts at: 1104 1906 3473 1104
Size: 802 1567 2232
Fragments arranged by size:
2232 1567 802
ApaI G_GGCC'C
Cuts at: 252 252
Size: 4601
ApaLI G'TGCA_C
Cuts at: 3371 3371
Size: 4601
ApoI r'AATT_y
Cuts at: 751 928 1605 3954 4210 751
Size: 177 677 2349 256 1142
Fragments arranged by size:
2349 1142 677 256 177
AseI AT'TA_AT
Cuts at: 20 20
Size: 4601
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
AvaI C'yCGr_G
Cuts at: 639 1332 1621 639
Size: 693 289 3619
Fragments arranged by size:
3619 693 289
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 512 545 512
Size: 33 4568
BamHI G'GATC_C
Cuts at: 1626 1626
Size: 4601
BanI G'GyrC_C
Cuts at: 190 376 439 562 1446 1479 1617 2031 2279
Size: 186 63 123 884 33 138 414 248
Cuts at: 2279 2314 190
Size: 35 2477
Fragments arranged by size:
2477 884 414 248 186 138 123 63
35 33
BanII G_rGCy'C
Cuts at: 252 854 1333 1615 2645 252
Size: 602 479 282 1030 2208
Fragments arranged by size:
2208 1030 602 479 282
BbeI G_GCGC'C
Cuts at: 194 380 443 1450 1483 2035 2283 194
Size: 186 63 1007 33 552 248 2512
Fragments arranged by size:
2512 1007 552 248 186 63 33
BbsI GAAGACnn'nnnn_
Cuts at: 577 577
Size: 4601
BbvCI CC'TCA_GC
Cuts at: 1093 1093
Size: 4601
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 469 503 469
Size: 34 4567
BciVI GTATCCnnnnn_n'
Cuts at: 2499 3260 4601 2499
Size: 761 1341 2499
Fragments arranged by size:
2499 1341 761
BclI T'GATC_A
Cuts at: 2121 2121
Size: 4601
BglI GCCn_nnn'nGGC
Cuts at: 247 385 511 1234 247
Size: 138 126 723 3614
Fragments arranged by size:
3614 723 138 126
BglII A'GATC_T
Cuts at: 2116 4153 2116
Size: 2037 2564
BlpI GC'TnA_GC
Cuts at: 1288 1713 1288
Size: 425 4176
Bme1580I G_kGCm'C
Cuts at: 252 2226 2319 3375 252
Size: 1974 93 1056 1478
Fragments arranged by size:
1974 1478 1056 93
BmgBI CAC'GTC
Cuts at: 653 653
Size: 4601
BmrI ACTGGGnnnn_n'
Cuts at: 1813 1894 2229 4373 1813
Size: 81 335 2144 2041
Fragments arranged by size:
2144 2041 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2968 2968
Size: 4601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 319 704 1204 1208 1339 202
Size: 117 385 500 4 131 3464
Fragments arranged by size:
3464 500 385 131 117 4
Bpu10I CC'TnA_GC
Cuts at: 764 1067 1093 764
Size: 303 26 4272
Fragments arranged by size:
4272 303 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1777 2656 3148 3446 1777
Size: 879 492 298 2932
Fragments arranged by size:
2932 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 475 894 4305 475
Size: 419 3411 771
Fragments arranged by size:
3411 771 419
BsaAI yAC'GTr
Cuts at: 198 1954 198
Size: 1756 2845
BsaBI GATnn'nnATC
Cuts at: 6 2115 4152 6
Size: 2109 2037 455
Fragments arranged by size:
2109 2037 455
BsaHI Gr'CG_yC
Cuts at: 191 377 440 503 972 1447 1480 1518 2032
Size: 186 63 63 469 475 33 38 514
Cuts at: 2032 2280 2982 4478 191
Size: 248 702 1496 314
Fragments arranged by size:
1496 702 514 475 469 314 248 186
63 63 38 33
BsaWI w'CCGG_w
Cuts at: 949 1790 2311 2989 3263 3410 3821 949
Size: 841 521 678 274 147 411 1729
Fragments arranged by size:
1729 841 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 278 308 1189 1206 1219 1236 1290 1320 278
Size: 30 881 17 13 17 54 30 3559
Fragments arranged by size:
3559 881 54 30 30 17 17 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 701 704 745 863 701
Size: 3 41 118 4439
Fragments arranged by size:
4439 118 41 3
BseYI C'CCAG_C
Cuts at: 1551 2026 2784 3361 3857 1551
Size: 475 758 577 496 2295
Fragments arranged by size:
2295 758 577 496 475
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 633 633
Size: 4601
BsiEI CG_ry'CG
Cuts at: 104 954 1372 2143 2189 3397 104
Size: 850 418 771 46 1208 1308
Fragments arranged by size:
1308 1208 850 771 418 46
BsiHKAI G_wGCw'C
Cuts at: 1615 2393 2583 3375 1615
Size: 778 190 792 2841
Fragments arranged by size:
2841 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1509 1509
Size: 4601
Bsp1286I G_dGCh'C
Cuts at: 252 854 1333 1615 2226 2319 2393 2583 2645
Size: 602 479 282 611 93 74 190 62
Cuts at: 2645 3375 252
Size: 730 1478
Fragments arranged by size:
1478 730 611 602 479 282 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3821 3821
Size: 4601
BspHI T'CATG_A
Cuts at: 3777 3777
Size: 4601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1651 1865 2167 2548 1651
Size: 214 302 381 3704
Fragments arranged by size:
3704 381 302 214
BsrBI CCG'CTC
Cuts at: 50 798 1323 2893 2947 4431 4596 50
Size: 748 525 1570 54 1484 165 55
Fragments arranged by size:
1570 1484 748 525 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2513 2513
Size: 4601
BsrFI r'CCGG_y
Cuts at: 949 1228 2599 2780 2989 949
Size: 279 1371 181 209 2561
Fragments arranged by size:
2561 1371 279 209 181
BssSI C'ACGA_G
Cuts at: 2872 3230 2872
Size: 358 4243
BstAPI GCAn_nnn'nTGC
Cuts at: 1978 1978
Size: 4601
BstBI TT'CG_AA
Cuts at: 2962 2962
Size: 4601
BstXI CCAn_nnnn'nTGG
Cuts at: 498 498
Size: 4601
BstYI r'GATC_y
Cuts at: 1 1541 1626 2093 2116 2451 2697 3709 3795
Size: 1540 85 467 23 335 246 1012 86
Cuts at: 3795 3807 4153 1
Size: 12 346 449
Fragments arranged by size:
1540 1012 467 449 346 335 246 86
85 23 12
Bsu36I CC'TnA_GG
Cuts at: 558 992 558
Size: 434 4167
BtgI C'CryG_G
Cuts at: 380 405 468 1006 380
Size: 25 63 538 3975
Fragments arranged by size:
3975 538 63 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2551 2732 2895 2551
Size: 181 163 4257
Fragments arranged by size:
4257 181 163
BtsI GCAGTG_nn'
Cuts at: 493 1844 493
Size: 1351 3250
DraI TTT'AAA
Cuts at: 1600 1600
Size: 4601
DrdI GACnn_nn'nnGTC
Cuts at: 421 2307 3165 421
Size: 1886 858 1857
Fragments arranged by size:
1886 1857 858
EaeI y'GGCC_r
Cuts at: 1369 2186 2360 2751 2778 4123 1369
Size: 817 174 391 27 1345 1847
Fragments arranged by size:
1847 1345 817 391 174 27
EagI C'GGCC_G
Cuts at: 1369 2186 1369
Size: 817 3784
EarI CTCTTCn'nnn_
Cuts at: 137 722 764 2624 2834 137
Size: 585 42 1860 210 1904
Fragments arranged by size:
1904 1860 585 210 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1027 1317 1534 2809 3119 3265 4411 1027
Size: 290 217 1275 310 146 1146 1217
Fragments arranged by size:
1275 1217 1146 310 290 217 146
EcoICRI GAG'CTC
Cuts at: 1613 1613
Size: 4601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 319 704 914 1070 1204 1208 1339 2426
Size: 117 385 210 156 134 4 131 1087
Cuts at: 2426 2858 3605 3873 202
Size: 432 747 268 930
Fragments arranged by size:
1087 930 747 432 385 268 210 156
134 131 117 4
EcoO109I rG'GnC_Cy
Cuts at: 615 858 988 996 1033 1740 615
Size: 243 130 8 37 707 3476
Fragments arranged by size:
3476 707 243 130 37 8
EcoRI G'AATT_C
Cuts at: 751 928 1605 751
Size: 177 677 3747
Fragments arranged by size:
3747 677 177
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 180 2620 180
Size: 2440 2161
FspI TGC'GCA
Cuts at: 215 2382 215
Size: 2167 2434
HaeII r_GCGC'y
Cuts at: 194 349 380 443 918 1191 1205 1450 1483
Size: 155 31 63 475 273 14 245 33
Cuts at: 1483 2035 2283 3305 4284 194
Size: 552 248 1022 979 511
Fragments arranged by size:
1022 979 552 511 475 273 248 245
155 63 33 31 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 766 798 893 925 1206 1238 1290 1322 1342
Size: 32 95 32 281 32 52 32 20
Cuts at: 1342 1374 2443 2473 2475 2505 766
Size: 32 1069 30 2 30 2862
Fragments arranged by size:
2862 1069 281 95 52 32 32 32
32 32 30 30 20 2
HincII GTy'rAC
Cuts at: 955 1214 1640 955
Size: 259 426 3916
Fragments arranged by size:
3916 426 259
KasI G'GCGC_C
Cuts at: 190 376 439 1446 1479 2031 2279 190
Size: 186 63 1007 33 552 248 2512
Fragments arranged by size:
2512 1007 552 248 186 63 33
KpnI G_GTAC'C
Cuts at: 1621 1621
Size: 4601
MluI A'CGCG_T
Cuts at: 4034 4034
Size: 4601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 693 3272 3456 693
Size: 2579 184 1838
Fragments arranged by size:
2579 1838 184
MscI TGG'CCA
Cuts at: 2362 2362
Size: 4601
MslI CAynn'nnrTG
Cuts at: 2590 2717 2590
Size: 127 4474
MspA1I CmG'CkG
Cuts at: 382 470 682 1712 2026 2386 3399 3644 382
Size: 88 212 1030 314 360 1013 245 1339
Fragments arranged by size:
1339 1030 1013 360 314 245 212 88
NaeI GCC'GGC
Cuts at: 1230 2782 1230
Size: 1552 3049
NarI GG'CG_CC
Cuts at: 191 377 440 1447 1480 2032 2280 191
Size: 186 63 1007 33 552 248 2512
Fragments arranged by size:
2512 1007 552 248 186 63 33
NcoI C'CATG_G
Cuts at: 1006 1006
Size: 4601
NgoMIV G'CCGG_C
Cuts at: 1228 2780 1228
Size: 1552 3049
NspI r_CATG'y
Cuts at: 1654 2154 3061 1654
Size: 500 907 3194
Fragments arranged by size:
3194 907 500
PasI CC'CwG_GG
Cuts at: 604 854 604
Size: 250 4351
PciI A'CATG_T
Cuts at: 3057 3057
Size: 4601
PflMI CCAn_nnn'nTGG
Cuts at: 462 462
Size: 4601
PfoI T'CCnGG_A
Cuts at: 296 1346 296
Size: 1050 3551
PmeI GTTT'AAAC
Cuts at: 1600 1600
Size: 4601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 4569
PpuMI rG'GwC_Cy
Cuts at: 615 996 1033 615
Size: 381 37 4183
Fragments arranged by size:
4183 381 37
PspOMI G'GGCC_C
Cuts at: 248 248
Size: 4601
PstI C_TGCA'G
Cuts at: 677 1648 2333 677
Size: 971 685 2945
Fragments arranged by size:
2945 971 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4601
PvuII CAG'CTG
Cuts at: 2026 2386 2026
Size: 360 4241
RsrII CG'GwC_CG
Cuts at: 2796 2796
Size: 4601
SacI G_AGCT'C
Cuts at: 1615 1615
Size: 4601
SacII CC_GC'GG
Cuts at: 383 471 383
Size: 88 4513
SalI G'TCGA_C
Cuts at: 953 1638 953
Size: 685 3916
SapI GCTCTTCn'nnn_
Cuts at: 137 2624 2834 137
Size: 2487 210 1904
Fragments arranged by size:
2487 1904 210
SbfI CC_TGCA'GG
Cuts at: 1648 1648
Size: 4601
ScaI AGT'ACT
Cuts at: 4118 4118
Size: 4601
SfcI C'TryA_G
Cuts at: 33 673 1644 2329 3322 3513 33
Size: 640 971 685 993 191 1121
Fragments arranged by size:
1121 993 971 685 640 191
SfoI GGC'GCC
Cuts at: 192 378 441 1448 1481 2033 2281 192
Size: 186 63 1007 33 552 248 2512
Fragments arranged by size:
2512 1007 552 248 186 63 33
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
SmaI CCC'GGG
Cuts at: 641 1623 641
Size: 982 3619
SmlI C'TyrA_G
Cuts at: 1756 2671 3163 3425 1756
Size: 915 492 262 2932
Fragments arranged by size:
2932 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4601
SphI G_CATG'C
Cuts at: 1654 2154 1654
Size: 500 4101
StyI C'CwwG_G
Cuts at: 515 1006 1735 2089 515
Size: 491 729 354 3027
Fragments arranged by size:
3027 729 491 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 344 903 2184 2317 2776 2985 344
Size: 559 1281 133 459 209 1960
Fragments arranged by size:
1960 1281 559 459 209 133
TatI w'GTAC_w
Cuts at: 4116 4116
Size: 4601
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4118 4150 4118
Size: 32 4569
Tth111I GACn'n_nGTC
Cuts at: 959 2398 959
Size: 1439 3162
XbaI T'CTAG_A
Cuts at: 1632 1632
Size: 4601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1506 1570 2096 1506
Size: 64 526 4011
Fragments arranged by size:
4011 526 64
XmaI C'CCGG_G
Cuts at: 639 1621 639
Size: 982 3619
ZraI GAC'GTC
Cuts at: 973 973
Size: 4601
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI AscI AvrII
BfrBI BmtI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI