Restriction map for
pF1KB8848
(Circular) (Six-base) MAPSORT of: pF1KB8848.seq Check: 5498 from: 1 to: 3314
pF1KB8848 3314 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 352 352
Size: 3314
Acc65I G'GTAC_C
Cuts at: 330 330
Size: 3314
AclI AA'CG_TT
Cuts at: 3154 3154
Size: 3314
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1139 1571 2318 2586 247
Size: 892 432 747 268 975
Fragments arranged by size:
975 892 747 432 268
AfeI AGC'GCT
Cuts at: 232 232
Size: 3314
AflIII A'CryG_T
Cuts at: 666 1770 2747 666
Size: 1104 977 1233
Fragments arranged by size:
1233 1104 977
AgeI A'CCGG_T
Cuts at: 1702 1702
Size: 3314
AlwNI CAG_nnn'CTG
Cuts at: 619 2186 619
Size: 1567 1747
ApaLI G'TGCA_C
Cuts at: 2084 2084
Size: 3314
ApoI r'AATT_y
Cuts at: 318 2667 2923 318
Size: 2349 256 709
Fragments arranged by size:
2349 709 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3314
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3314
AvaI C'yCGr_G
Cuts at: 334 334
Size: 3314
BamHI G'GATC_C
Cuts at: 339 339
Size: 3314
BanI G'GyrC_C
Cuts at: 330 744 992 1027 330
Size: 414 248 35 2617
Fragments arranged by size:
2617 414 248 35
BanII G_rGCy'C
Cuts at: 328 1358 328
Size: 1030 2284
BbeI G_GCGC'C
Cuts at: 748 996 748
Size: 248 3066
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 129 163 187 221 129
Size: 34 24 34 3222
Fragments arranged by size:
3222 34 34 24
BciVI GTATCCnnnnn_n'
Cuts at: 1212 1973 3314 1212
Size: 761 1341 1212
Fragments arranged by size:
1341 1212 761
BclI T'GATC_A
Cuts at: 110 834 110
Size: 724 2590
BglII A'GATC_T
Cuts at: 829 2866 829
Size: 2037 1277
BlpI GC'TnA_GC
Cuts at: 426 426
Size: 3314
Bme1580I G_kGCm'C
Cuts at: 939 1032 2088 939
Size: 93 1056 2165
Fragments arranged by size:
2165 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 526 607 942 3086 526
Size: 81 335 2144 754
Fragments arranged by size:
2144 754 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1681 1681
Size: 3314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 322
Size: 3314
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 1369 1861 2159 490
Size: 879 492 298 1645
Fragments arranged by size:
1645 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3018 3018
Size: 3314
BsaAI yAC'GTr
Cuts at: 264 667 264
Size: 403 2911
BsaBI GATnn'nnATC
Cuts at: 6 828 2865 6
Size: 822 2037 455
Fragments arranged by size:
2037 822 455
BsaHI Gr'CG_yC
Cuts at: 745 993 1695 3191 745
Size: 248 702 1496 868
Fragments arranged by size:
1496 868 702 248
BsaWI w'CCGG_w
Cuts at: 503 1024 1702 1976 2123 2534 503
Size: 521 678 274 147 411 1283
Fragments arranged by size:
1283 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 739 1497 2074 2570 739
Size: 758 577 496 1483
Fragments arranged by size:
1483 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 856 902 2110 104
Size: 752 46 1208 1308
Fragments arranged by size:
1308 1208 752 46
BsiHKAI G_wGCw'C
Cuts at: 328 1106 1296 2088 328
Size: 778 190 792 1554
Fragments arranged by size:
1554 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 328 939 1032 1106 1296 1358 2088 328
Size: 611 93 74 190 62 730 1554
Fragments arranged by size:
1554 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2534 2534
Size: 3314
BspHI T'CATG_A
Cuts at: 2490 2490
Size: 3314
BspMI ACCTGCnnnn'nnnn_
Cuts at: 364 578 880 1261 364
Size: 214 302 381 2417
Fragments arranged by size:
2417 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1606 1660 3144 3309 50
Size: 1556 54 1484 165 55
Fragments arranged by size:
1556 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1226 1226
Size: 3314
BsrFI r'CCGG_y
Cuts at: 1312 1493 1702 1312
Size: 181 209 2924
Fragments arranged by size:
2924 209 181
BssSI C'ACGA_G
Cuts at: 1585 1943 1585
Size: 358 2956
BstAPI GCAn_nnn'nTGC
Cuts at: 691 691
Size: 3314
BstBI TT'CG_AA
Cuts at: 1675 1675
Size: 3314
BstYI r'GATC_y
Cuts at: 1 339 806 829 1164 1410 2422 2508 2520
Size: 338 467 23 335 246 1012 86 12
Cuts at: 2520 2866 1
Size: 346 449
Fragments arranged by size:
1012 467 449 346 338 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1264 1445 1608 1264
Size: 181 163 2970
Fragments arranged by size:
2970 181 163
BtsI GCAGTG_nn'
Cuts at: 557 557
Size: 3314
DraI TTT'AAA
Cuts at: 313 313
Size: 3314
DrdI GACnn_nn'nnGTC
Cuts at: 1020 1878 1020
Size: 858 2456
EaeI y'GGCC_r
Cuts at: 899 1073 1464 1491 2836 899
Size: 174 391 27 1345 1377
Fragments arranged by size:
1377 1345 391 174 27
EagI C'GGCC_G
Cuts at: 899 899
Size: 3314
EarI CTCTTCn'nnn_
Cuts at: 1337 1547 1337
Size: 210 3104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1522 1832 1978 3124 1522
Size: 310 146 1146 1712
Fragments arranged by size:
1712 1146 310 146
EcoICRI GAG'CTC
Cuts at: 326 326
Size: 3314
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 322 1139 1571 2318 2586 247
Size: 75 817 432 747 268 975
Fragments arranged by size:
975 817 747 432 268 75
EcoNI CCTnn'n_nnAGG
Cuts at: 304 304
Size: 3314
EcoO109I rG'GnC_Cy
Cuts at: 453 453
Size: 3314
EcoRI G'AATT_C
Cuts at: 318 318
Size: 3314
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1333 1333
Size: 3314
FspI TGC'GCA
Cuts at: 1095 1095
Size: 3314
HaeII r_GCGC'y
Cuts at: 234 748 996 2018 2997 234
Size: 514 248 1022 979 551
Fragments arranged by size:
1022 979 551 514 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1156 1186 1188 1218 1156
Size: 30 2 30 3252
Fragments arranged by size:
3252 30 30 2
HincII GTy'rAC
Cuts at: 353 353
Size: 3314
KasI G'GCGC_C
Cuts at: 744 992 744
Size: 248 3066
KpnI G_GTAC'C
Cuts at: 334 334
Size: 3314
MluI A'CGCG_T
Cuts at: 2747 2747
Size: 3314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1985 2169 1985
Size: 184 3130
MscI TGG'CCA
Cuts at: 1075 1075
Size: 3314
MslI CAynn'nnrTG
Cuts at: 1303 1430 1303
Size: 127 3187
MspA1I CmG'CkG
Cuts at: 425 739 1099 2112 2357 425
Size: 314 360 1013 245 1382
Fragments arranged by size:
1382 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1495 1495
Size: 3314
NarI GG'CG_CC
Cuts at: 745 993 745
Size: 248 3066
NgoMIV G'CCGG_C
Cuts at: 1493 1493
Size: 3314
NspI r_CATG'y
Cuts at: 367 867 1774 367
Size: 500 907 1907
Fragments arranged by size:
1907 907 500
PasI CC'CwG_GG
Cuts at: 219 219
Size: 3314
PciI A'CATG_T
Cuts at: 1770 1770
Size: 3314
PmeI GTTT'AAAC
Cuts at: 313 313
Size: 3314
PmlI CAC'GTG
Cuts at: 264 264
Size: 3314
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2453 2485 2453
Size: 32 3282
PstI C_TGCA'G
Cuts at: 185 361 1046 185
Size: 176 685 2453
Fragments arranged by size:
2453 685 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3314
PvuII CAG'CTG
Cuts at: 739 1099 739
Size: 360 2954
RsrII CG'GwC_CG
Cuts at: 1509 1509
Size: 3314
SacI G_AGCT'C
Cuts at: 328 328
Size: 3314
SalI G'TCGA_C
Cuts at: 351 351
Size: 3314
SapI GCTCTTCn'nnn_
Cuts at: 1337 1547 1337
Size: 210 3104
SbfI CC_TGCA'GG
Cuts at: 361 361
Size: 3314
ScaI AGT'ACT
Cuts at: 2831 2831
Size: 3314
SfcI C'TryA_G
Cuts at: 33 181 357 1042 2035 2226 33
Size: 148 176 685 993 191 1121
Fragments arranged by size:
1121 993 685 191 176 148
SfoI GGC'GCC
Cuts at: 746 994 746
Size: 248 3066
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3314
SmaI CCC'GGG
Cuts at: 336 336
Size: 3314
SmlI C'TyrA_G
Cuts at: 469 1384 1876 2138 469
Size: 915 492 262 1645
Fragments arranged by size:
1645 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3314
SphI G_CATG'C
Cuts at: 367 867 367
Size: 500 2814
StyI C'CwwG_G
Cuts at: 448 802 448
Size: 354 2960
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 897 1030 1489 1698 897
Size: 133 459 209 2513
Fragments arranged by size:
2513 459 209 133
TatI w'GTAC_w
Cuts at: 191 2829 191
Size: 2638 676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2831 2863 2831
Size: 32 3282
Tth111I GACn'n_nGTC
Cuts at: 1111 1111
Size: 3314
XbaI T'CTAG_A
Cuts at: 345 345
Size: 3314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 809 809
Size: 3314
XmaI C'CCGG_G
Cuts at: 334 334
Size: 3314
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI