Restriction map for
pF1KB8852
(Circular) (Six-base) MAPSORT of: pF1KB8852.seq Check: 9347 from: 1 to: 3338
pF1KB8852 3338 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AccI GT'mk_AC
Cuts at: 376 376
Size: 3338
Acc65I G'GTAC_C
Cuts at: 354 354
Size: 3338
AclI AA'CG_TT
Cuts at: 3178 3178
Size: 3338
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1595 2342 2610 1163
Size: 432 747 268 1891
Fragments arranged by size:
1891 747 432 268
AflII C'TTAA_G
Cuts at: 175 175
Size: 3338
AflIII A'CryG_T
Cuts at: 690 1794 2771 690
Size: 1104 977 1257
Fragments arranged by size:
1257 1104 977
AgeI A'CCGG_T
Cuts at: 1726 1726
Size: 3338
AleI CACnn'nnGTG
Cuts at: 254 277 254
Size: 23 3315
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 298 330 298
Size: 32 3306
AlwNI CAG_nnn'CTG
Cuts at: 643 2210 643
Size: 1567 1771
ApaLI G'TGCA_C
Cuts at: 2108 2108
Size: 3338
ApoI r'AATT_y
Cuts at: 342 2691 2947 342
Size: 2349 256 733
Fragments arranged by size:
2349 733 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3338
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3338
AvaI C'yCGr_G
Cuts at: 358 358
Size: 3338
BamHI G'GATC_C
Cuts at: 261 363 261
Size: 102 3236
BanI G'GyrC_C
Cuts at: 354 768 1016 1051 354
Size: 414 248 35 2641
Fragments arranged by size:
2641 414 248 35
BanII G_rGCy'C
Cuts at: 352 1382 352
Size: 1030 2308
BbeI G_GCGC'C
Cuts at: 772 1020 772
Size: 248 3090
BciVI GTATCCnnnnn_n'
Cuts at: 1236 1997 3338 1236
Size: 761 1341 1236
Fragments arranged by size:
1341 1236 761
BclI T'GATC_A
Cuts at: 858 858
Size: 3338
BglII A'GATC_T
Cuts at: 853 2890 853
Size: 2037 1301
BlpI GC'TnA_GC
Cuts at: 450 450
Size: 3338
Bme1580I G_kGCm'C
Cuts at: 284 963 1056 2112 284
Size: 679 93 1056 1510
Fragments arranged by size:
1510 1056 679 93
BmrI ACTGGGnnnn_n'
Cuts at: 550 631 966 3110 550
Size: 81 335 2144 778
Fragments arranged by size:
2144 778 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1705 1705
Size: 3338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 1393 1885 2183 514
Size: 879 492 298 1669
Fragments arranged by size:
1669 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 112 3042 112
Size: 2930 408
BsaAI yAC'GTr
Cuts at: 691 691
Size: 3338
BsaBI GATnn'nnATC
Cuts at: 6 852 2889 6
Size: 846 2037 455
Fragments arranged by size:
2037 846 455
BsaHI Gr'CG_yC
Cuts at: 769 1017 1719 3215 769
Size: 248 702 1496 892
Fragments arranged by size:
1496 892 702 248
BsaWI w'CCGG_w
Cuts at: 527 1048 1726 2000 2147 2558 527
Size: 521 678 274 147 411 1307
Fragments arranged by size:
1307 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 298 328 298
Size: 30 3308
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 319 319
Size: 3338
BseYI C'CCAG_C
Cuts at: 763 1521 2098 2594 763
Size: 758 577 496 1507
Fragments arranged by size:
1507 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 880 926 2134 104
Size: 776 46 1208 1308
Fragments arranged by size:
1308 1208 776 46
BsiHKAI G_wGCw'C
Cuts at: 327 352 1130 1320 2112 327
Size: 25 778 190 792 1553
Fragments arranged by size:
1553 792 778 190 25
Bsp1286I G_dGCh'C
Cuts at: 284 327 352 963 1056 1130 1320 1382 2112
Size: 43 25 611 93 74 190 62 730
Cuts at: 2112 284
Size: 1510
Fragments arranged by size:
1510 730 611 190 93 74 62 43
25
BspEI T'CCGG_A
Cuts at: 2558 2558
Size: 3338
BspHI T'CATG_A
Cuts at: 2514 2514
Size: 3338
BspMI ACCTGCnnnn'nnnn_
Cuts at: 388 602 904 1285 388
Size: 214 302 381 2441
Fragments arranged by size:
2441 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1630 1684 3168 3333 50
Size: 1580 54 1484 165 55
Fragments arranged by size:
1580 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1250 1250
Size: 3338
BsrFI r'CCGG_y
Cuts at: 1336 1517 1726 1336
Size: 181 209 2948
Fragments arranged by size:
2948 209 181
BssSI C'ACGA_G
Cuts at: 1609 1967 1609
Size: 358 2980
BstAPI GCAn_nnn'nTGC
Cuts at: 715 715
Size: 3338
BstBI TT'CG_AA
Cuts at: 1699 1699
Size: 3338
BstYI r'GATC_y
Cuts at: 1 261 363 830 853 1188 1434 2446 2532
Size: 260 102 467 23 335 246 1012 86
Cuts at: 2532 2544 2890 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 260 246 102
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1288 1469 1632 1288
Size: 181 163 2994
Fragments arranged by size:
2994 181 163
BtsI GCAGTG_nn'
Cuts at: 581 581
Size: 3338
DraI TTT'AAA
Cuts at: 337 337
Size: 3338
DrdI GACnn_nn'nnGTC
Cuts at: 1044 1902 1044
Size: 858 2480
EaeI y'GGCC_r
Cuts at: 923 1097 1488 1515 2860 923
Size: 174 391 27 1345 1401
Fragments arranged by size:
1401 1345 391 174 27
EagI C'GGCC_G
Cuts at: 923 923
Size: 3338
EarI CTCTTCn'nnn_
Cuts at: 1361 1571 1361
Size: 210 3128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1546 1856 2002 3148 1546
Size: 310 146 1146 1736
Fragments arranged by size:
1736 1146 310 146
EcoICRI GAG'CTC
Cuts at: 350 350
Size: 3338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1595 2342 2610 1163
Size: 432 747 268 1891
Fragments arranged by size:
1891 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 477 477
Size: 3338
EcoRI G'AATT_C
Cuts at: 342 342
Size: 3338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 333 1357 333
Size: 1024 2314
FspI TGC'GCA
Cuts at: 1119 1119
Size: 3338
HaeII r_GCGC'y
Cuts at: 772 1020 2042 3021 772
Size: 248 1022 979 1089
Fragments arranged by size:
1089 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1180 1210 1212 1242 1180
Size: 30 2 30 3276
Fragments arranged by size:
3276 30 30 2
HincII GTy'rAC
Cuts at: 377 377
Size: 3338
KasI G'GCGC_C
Cuts at: 768 1016 768
Size: 248 3090
KpnI G_GTAC'C
Cuts at: 358 358
Size: 3338
MluI A'CGCG_T
Cuts at: 2771 2771
Size: 3338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 178 2009 2193 178
Size: 1831 184 1323
Fragments arranged by size:
1831 1323 184
MscI TGG'CCA
Cuts at: 1099 1099
Size: 3338
MslI CAynn'nnrTG
Cuts at: 254 277 1327 1454 254
Size: 23 1050 127 2138
Fragments arranged by size:
2138 1050 127 23
MspA1I CmG'CkG
Cuts at: 449 763 1123 2136 2381 449
Size: 314 360 1013 245 1406
Fragments arranged by size:
1406 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1519 1519
Size: 3338
NarI GG'CG_CC
Cuts at: 769 1017 769
Size: 248 3090
NgoMIV G'CCGG_C
Cuts at: 1517 1517
Size: 3338
NspI r_CATG'y
Cuts at: 391 891 1798 391
Size: 500 907 1931
Fragments arranged by size:
1931 907 500
PciI A'CATG_T
Cuts at: 1794 1794
Size: 3338
PfoI T'CCnGG_A
Cuts at: 302 302
Size: 3338
PmeI GTTT'AAAC
Cuts at: 337 337
Size: 3338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 298 330 2477 2509 298
Size: 32 2147 32 1127
Fragments arranged by size:
2147 1127 32 32
PstI C_TGCA'G
Cuts at: 385 1070 385
Size: 685 2653
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3338
PvuII CAG'CTG
Cuts at: 763 1123 763
Size: 360 2978
RsrII CG'GwC_CG
Cuts at: 1533 1533
Size: 3338
SacI G_AGCT'C
Cuts at: 352 352
Size: 3338
SalI G'TCGA_C
Cuts at: 375 375
Size: 3338
SapI GCTCTTCn'nnn_
Cuts at: 1361 1571 1361
Size: 210 3128
SbfI CC_TGCA'GG
Cuts at: 385 385
Size: 3338
ScaI AGT'ACT
Cuts at: 2855 2855
Size: 3338
SfcI C'TryA_G
Cuts at: 33 381 1066 2059 2250 33
Size: 348 685 993 191 1121
Fragments arranged by size:
1121 993 685 348 191
SfoI GGC'GCC
Cuts at: 770 1018 770
Size: 248 3090
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3338
SmaI CCC'GGG
Cuts at: 360 360
Size: 3338
SmlI C'TyrA_G
Cuts at: 175 493 1408 1900 2162 175
Size: 318 915 492 262 1351
Fragments arranged by size:
1351 915 492 318 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3338
SphI G_CATG'C
Cuts at: 391 891 391
Size: 500 2838
StyI C'CwwG_G
Cuts at: 212 472 826 212
Size: 260 354 2724
Fragments arranged by size:
2724 354 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 921 1054 1513 1722 921
Size: 133 459 209 2537
Fragments arranged by size:
2537 459 209 133
TatI w'GTAC_w
Cuts at: 2853 2853
Size: 3338
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2855 2887 2855
Size: 32 3306
Tth111I GACn'n_nGTC
Cuts at: 1135 1135
Size: 3338
XbaI T'CTAG_A
Cuts at: 369 369
Size: 3338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 833 833
Size: 3338
XmaI C'CCGG_G
Cuts at: 358 358
Size: 3338
XmnI GAAnn'nnTTC
Cuts at: 298 313 316 298
Size: 15 3 3320
Fragments arranged by size:
3320 15 3
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI