Restriction map for
pF1KB8856
(Circular) (Six-base) MAPSORT of: pF1KB8856.seq Check: 7654 from: 1 to: 3383
pF1KB8856 3383 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 421 421
Size: 3383
Acc65I G'GTAC_C
Cuts at: 399 399
Size: 3383
AclI AA'CG_TT
Cuts at: 3223 3223
Size: 3383
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 1208 1640 2387 2655 229
Size: 979 432 747 268 957
Fragments arranged by size:
979 957 747 432 268
AflIII A'CryG_T
Cuts at: 735 1839 2816 735
Size: 1104 977 1302
Fragments arranged by size:
1302 1104 977
AgeI A'CCGG_T
Cuts at: 1771 1771
Size: 3383
AlwNI CAG_nnn'CTG
Cuts at: 688 2255 688
Size: 1567 1816
ApaLI G'TGCA_C
Cuts at: 2153 2153
Size: 3383
ApoI r'AATT_y
Cuts at: 387 2736 2992 387
Size: 2349 256 778
Fragments arranged by size:
2349 778 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3383
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3383
AvaI C'yCGr_G
Cuts at: 403 403
Size: 3383
BamHI G'GATC_C
Cuts at: 408 408
Size: 3383
BanI G'GyrC_C
Cuts at: 399 813 1061 1096 399
Size: 414 248 35 2686
Fragments arranged by size:
2686 414 248 35
BanII G_rGCy'C
Cuts at: 233 397 1427 233
Size: 164 1030 2189
Fragments arranged by size:
2189 1030 164
BbeI G_GCGC'C
Cuts at: 817 1065 817
Size: 248 3135
BbsI GAAGACnn'nnnn_
Cuts at: 287 287
Size: 3383
BciVI GTATCCnnnnn_n'
Cuts at: 1281 2042 3383 1281
Size: 761 1341 1281
Fragments arranged by size:
1341 1281 761
BclI T'GATC_A
Cuts at: 903 903
Size: 3383
BglII A'GATC_T
Cuts at: 898 2935 898
Size: 2037 1346
BlpI GC'TnA_GC
Cuts at: 495 495
Size: 3383
Bme1580I G_kGCm'C
Cuts at: 1008 1101 2157 1008
Size: 93 1056 2234
Fragments arranged by size:
2234 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 595 676 1011 3155 595
Size: 81 335 2144 823
Fragments arranged by size:
2144 823 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1750 1750
Size: 3383
Bpu10I CC'TnA_GC
Cuts at: 193 193
Size: 3383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 559 1438 1930 2228 355
Size: 204 879 492 298 1510
Fragments arranged by size:
1510 879 492 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 3087 3087
Size: 3383
BsaAI yAC'GTr
Cuts at: 736 736
Size: 3383
BsaBI GATnn'nnATC
Cuts at: 6 897 2934 6
Size: 891 2037 455
Fragments arranged by size:
2037 891 455
BsaHI Gr'CG_yC
Cuts at: 814 1062 1764 3260 814
Size: 248 702 1496 937
Fragments arranged by size:
1496 937 702 248
BsaWI w'CCGG_w
Cuts at: 290 572 1093 1771 2045 2192 2603 290
Size: 282 521 678 274 147 411 1070
Fragments arranged by size:
1070 678 521 411 282 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 148 178 148
Size: 30 3353
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 130
Size: 3383
BseYI C'CCAG_C
Cuts at: 808 1566 2143 2639 808
Size: 758 577 496 1552
Fragments arranged by size:
1552 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 925 971 2179 104
Size: 821 46 1208 1308
Fragments arranged by size:
1308 1208 821 46
BsiHKAI G_wGCw'C
Cuts at: 233 397 1175 1365 2157 233
Size: 164 778 190 792 1459
Fragments arranged by size:
1459 792 778 190 164
Bsp1286I G_dGCh'C
Cuts at: 233 397 1008 1101 1175 1365 1427 2157 233
Size: 164 611 93 74 190 62 730 1459
Fragments arranged by size:
1459 730 611 190 164 93 74 62
BspEI T'CCGG_A
Cuts at: 2603 2603
Size: 3383
BspHI T'CATG_A
Cuts at: 2559 2559
Size: 3383
BspMI ACCTGCnnnn'nnnn_
Cuts at: 433 647 949 1330 433
Size: 214 302 381 2486
Fragments arranged by size:
2486 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1675 1729 3213 3378 50
Size: 1625 54 1484 165 55
Fragments arranged by size:
1625 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1295 1295
Size: 3383
BsrFI r'CCGG_y
Cuts at: 1381 1562 1771 1381
Size: 181 209 2993
Fragments arranged by size:
2993 209 181
BssSI C'ACGA_G
Cuts at: 142 1654 2012 142
Size: 1512 358 1513
Fragments arranged by size:
1513 1512 358
BstAPI GCAn_nnn'nTGC
Cuts at: 760 760
Size: 3383
BstBI TT'CG_AA
Cuts at: 1744 1744
Size: 3383
BstYI r'GATC_y
Cuts at: 1 408 875 898 1233 1479 2491 2577 2589
Size: 407 467 23 335 246 1012 86 12
Cuts at: 2589 2935 1
Size: 346 449
Fragments arranged by size:
1012 467 449 407 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3383
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1333 1514 1677 1333
Size: 181 163 3039
Fragments arranged by size:
3039 181 163
BtsI GCAGTG_nn'
Cuts at: 626 626
Size: 3383
DraI TTT'AAA
Cuts at: 382 382
Size: 3383
DrdI GACnn_nn'nnGTC
Cuts at: 1089 1947 1089
Size: 858 2525
EaeI y'GGCC_r
Cuts at: 146 968 1142 1533 1560 2905 146
Size: 822 174 391 27 1345 624
Fragments arranged by size:
1345 822 624 391 174 27
EagI C'GGCC_G
Cuts at: 968 968
Size: 3383
EarI CTCTTCn'nnn_
Cuts at: 1406 1616 1406
Size: 210 3173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1591 1901 2047 3193 1591
Size: 310 146 1146 1781
Fragments arranged by size:
1781 1146 310 146
EcoICRI GAG'CTC
Cuts at: 231 395 231
Size: 164 3219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 1208 1640 2387 2655 229
Size: 979 432 747 268 957
Fragments arranged by size:
979 957 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 124 124
Size: 3383
EcoO109I rG'GnC_Cy
Cuts at: 341 522 341
Size: 181 3202
EcoRI G'AATT_C
Cuts at: 387 387
Size: 3383
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1402 1402
Size: 3383
FspI TGC'GCA
Cuts at: 1164 1164
Size: 3383
HaeII r_GCGC'y
Cuts at: 817 1065 2087 3066 817
Size: 248 1022 979 1134
Fragments arranged by size:
1134 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1225 1255 1257 1287 1225
Size: 30 2 30 3321
Fragments arranged by size:
3321 30 30 2
HincII GTy'rAC
Cuts at: 422 422
Size: 3383
KasI G'GCGC_C
Cuts at: 813 1061 813
Size: 248 3135
KpnI G_GTAC'C
Cuts at: 403 403
Size: 3383
MluI A'CGCG_T
Cuts at: 2816 2816
Size: 3383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2054 2238 2054
Size: 184 3199
MscI TGG'CCA
Cuts at: 148 1144 148
Size: 996 2387
MslI CAynn'nnrTG
Cuts at: 1372 1499 1372
Size: 127 3256
MspA1I CmG'CkG
Cuts at: 494 808 1168 2181 2426 494
Size: 314 360 1013 245 1451
Fragments arranged by size:
1451 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1564 1564
Size: 3383
NarI GG'CG_CC
Cuts at: 814 1062 814
Size: 248 3135
NcoI C'CATG_G
Cuts at: 107 107
Size: 3383
NgoMIV G'CCGG_C
Cuts at: 1562 1562
Size: 3383
NspI r_CATG'y
Cuts at: 116 436 936 1843 116
Size: 320 500 907 1656
Fragments arranged by size:
1656 907 500 320
PciI A'CATG_T
Cuts at: 1839 1839
Size: 3383
PfoI T'CCnGG_A
Cuts at: 317 317
Size: 3383
PmeI GTTT'AAAC
Cuts at: 382 382
Size: 3383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2522 2554 2522
Size: 32 3351
PstI C_TGCA'G
Cuts at: 254 430 1115 254
Size: 176 685 2522
Fragments arranged by size:
2522 685 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3383
PvuII CAG'CTG
Cuts at: 808 1168 808
Size: 360 3023
RsrII CG'GwC_CG
Cuts at: 1578 1578
Size: 3383
SacI G_AGCT'C
Cuts at: 233 397 233
Size: 164 3219
SalI G'TCGA_C
Cuts at: 420 420
Size: 3383
SapI GCTCTTCn'nnn_
Cuts at: 1406 1616 1406
Size: 210 3173
SbfI CC_TGCA'GG
Cuts at: 430 430
Size: 3383
ScaI AGT'ACT
Cuts at: 163 2900 163
Size: 2737 646
SfcI C'TryA_G
Cuts at: 33 250 426 1111 2104 2295 33
Size: 217 176 685 993 191 1121
Fragments arranged by size:
1121 993 685 217 191 176
SfoI GGC'GCC
Cuts at: 815 1063 815
Size: 248 3135
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3383
SmaI CCC'GGG
Cuts at: 405 405
Size: 3383
SmlI C'TyrA_G
Cuts at: 370 538 1453 1945 2207 370
Size: 168 915 492 262 1546
Fragments arranged by size:
1546 915 492 262 168
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3383
SphI G_CATG'C
Cuts at: 116 436 936 116
Size: 320 500 2563
Fragments arranged by size:
2563 500 320
StyI C'CwwG_G
Cuts at: 107 344 517 871 107
Size: 237 173 354 2619
Fragments arranged by size:
2619 354 237 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 966 1099 1558 1767 966
Size: 133 459 209 2582
Fragments arranged by size:
2582 459 209 133
TatI w'GTAC_w
Cuts at: 161 2898 161
Size: 2737 646
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 148 180 2900 2932 148
Size: 32 2720 32 599
Fragments arranged by size:
2720 599 32 32
Tth111I GACn'n_nGTC
Cuts at: 1180 1180
Size: 3383
XbaI T'CTAG_A
Cuts at: 414 414
Size: 3383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 878 878
Size: 3383
XmaI C'CCGG_G
Cuts at: 403 403
Size: 3383
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI