Restriction map for
pF1KB8863
(Circular) (Six-base) MAPSORT of: pF1KB8863.seq Check: 9074 from: 1 to: 3416
pF1KB8863 3416 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 454 454
Size: 3416
Acc65I G'GTAC_C
Cuts at: 432 432
Size: 3416
AclI AA'CG_TT
Cuts at: 3256 3256
Size: 3416
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1241 1673 2420 2688 1241
Size: 432 747 268 1969
Fragments arranged by size:
1969 747 432 268
AflIII A'CryG_T
Cuts at: 768 1872 2849 768
Size: 1104 977 1335
Fragments arranged by size:
1335 1104 977
AgeI A'CCGG_T
Cuts at: 1804 1804
Size: 3416
AlwNI CAG_nnn'CTG
Cuts at: 279 721 2288 279
Size: 442 1567 1407
Fragments arranged by size:
1567 1407 442
ApaLI G'TGCA_C
Cuts at: 2186 2186
Size: 3416
ApoI r'AATT_y
Cuts at: 370 420 2769 3025 370
Size: 50 2349 256 761
Fragments arranged by size:
2349 761 256 50
AseI AT'TA_AT
Cuts at: 20 20
Size: 3416
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3416
AvaI C'yCGr_G
Cuts at: 165 224 436 165
Size: 59 212 3145
Fragments arranged by size:
3145 212 59
BamHI G'GATC_C
Cuts at: 441 441
Size: 3416
BanI G'GyrC_C
Cuts at: 432 846 1094 1129 432
Size: 414 248 35 2719
Fragments arranged by size:
2719 414 248 35
BanII G_rGCy'C
Cuts at: 430 1460 430
Size: 1030 2386
BbeI G_GCGC'C
Cuts at: 850 1098 850
Size: 248 3168
BbsI GAAGACnn'nnnn_
Cuts at: 330 330
Size: 3416
BciVI GTATCCnnnnn_n'
Cuts at: 1314 2075 3416 1314
Size: 761 1341 1314
Fragments arranged by size:
1341 1314 761
BclI T'GATC_A
Cuts at: 936 936
Size: 3416
BglII A'GATC_T
Cuts at: 931 2968 931
Size: 2037 1379
BlpI GC'TnA_GC
Cuts at: 528 528
Size: 3416
Bme1580I G_kGCm'C
Cuts at: 118 1041 1134 2190 118
Size: 923 93 1056 1344
Fragments arranged by size:
1344 1056 923 93
BmrI ACTGGGnnnn_n'
Cuts at: 628 709 1044 3188 628
Size: 81 335 2144 856
Fragments arranged by size:
2144 856 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1783 1783
Size: 3416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 142
Size: 3416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 1471 1963 2261 592
Size: 879 492 298 1747
Fragments arranged by size:
1747 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3120 3120
Size: 3416
BsaAI yAC'GTr
Cuts at: 769 769
Size: 3416
BsaBI GATnn'nnATC
Cuts at: 6 930 2967 6
Size: 924 2037 455
Fragments arranged by size:
2037 924 455
BsaHI Gr'CG_yC
Cuts at: 847 1095 1797 3293 847
Size: 248 702 1496 970
Fragments arranged by size:
1496 970 702 248
BsaWI w'CCGG_w
Cuts at: 605 1126 1804 2078 2225 2636 605
Size: 521 678 274 147 411 1385
Fragments arranged by size:
1385 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 235 841 1599 2176 2672 235
Size: 606 758 577 496 979
Fragments arranged by size:
979 758 606 577 496
BsiEI CG_ry'CG
Cuts at: 104 958 1004 2212 104
Size: 854 46 1208 1308
Fragments arranged by size:
1308 1208 854 46
BsiHKAI G_wGCw'C
Cuts at: 430 1208 1398 2190 430
Size: 778 190 792 1656
Fragments arranged by size:
1656 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 118 430 1041 1134 1208 1398 1460 2190 118
Size: 312 611 93 74 190 62 730 1344
Fragments arranged by size:
1344 730 611 312 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2636 2636
Size: 3416
BspHI T'CATG_A
Cuts at: 2592 2592
Size: 3416
BspMI ACCTGCnnnn'nnnn_
Cuts at: 466 680 982 1363 466
Size: 214 302 381 2519
Fragments arranged by size:
2519 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1708 1762 3246 3411 50
Size: 1658 54 1484 165 55
Fragments arranged by size:
1658 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1328 1328
Size: 3416
BsrFI r'CCGG_y
Cuts at: 1414 1595 1804 1414
Size: 181 209 3026
Fragments arranged by size:
3026 209 181
BssSI C'ACGA_G
Cuts at: 1687 2045 1687
Size: 358 3058
BstAPI GCAn_nnn'nTGC
Cuts at: 793 793
Size: 3416
BstBI TT'CG_AA
Cuts at: 1777 1777
Size: 3416
BstXI CCAn_nnnn'nTGG
Cuts at: 243 243
Size: 3416
BstYI r'GATC_y
Cuts at: 1 441 908 931 1266 1512 2524 2610 2622
Size: 440 467 23 335 246 1012 86 12
Cuts at: 2622 2968 1
Size: 346 449
Fragments arranged by size:
1012 467 449 440 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3416
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 336 1366 1547 1710 336
Size: 1030 181 163 2042
Fragments arranged by size:
2042 1030 181 163
BtsI GCAGTG_nn'
Cuts at: 659 659
Size: 3416
DraI TTT'AAA
Cuts at: 415 415
Size: 3416
DrdI GACnn_nn'nnGTC
Cuts at: 1122 1980 1122
Size: 858 2558
EaeI y'GGCC_r
Cuts at: 1001 1175 1566 1593 2938 1001
Size: 174 391 27 1345 1479
Fragments arranged by size:
1479 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1001 1001
Size: 3416
EarI CTCTTCn'nnn_
Cuts at: 1439 1649 1439
Size: 210 3206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1624 1934 2080 3226 1624
Size: 310 146 1146 1814
Fragments arranged by size:
1814 1146 310 146
EcoICRI GAG'CTC
Cuts at: 428 428
Size: 3416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1241 1673 2420 2688 142
Size: 1099 432 747 268 870
Fragments arranged by size:
1099 870 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 129 555 129
Size: 426 2990
EcoRI G'AATT_C
Cuts at: 370 420 370
Size: 50 3366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1435 1435
Size: 3416
FspI TGC'GCA
Cuts at: 1197 1197
Size: 3416
HaeII r_GCGC'y
Cuts at: 850 1098 2120 3099 850
Size: 248 1022 979 1167
Fragments arranged by size:
1167 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1258 1288 1290 1320 1258
Size: 30 2 30 3354
Fragments arranged by size:
3354 30 30 2
HincII GTy'rAC
Cuts at: 455 455
Size: 3416
KasI G'GCGC_C
Cuts at: 846 1094 846
Size: 248 3168
KpnI G_GTAC'C
Cuts at: 436 436
Size: 3416
MluI A'CGCG_T
Cuts at: 2849 2849
Size: 3416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2087 2271 2087
Size: 184 3232
MscI TGG'CCA
Cuts at: 1177 1177
Size: 3416
MslI CAynn'nnrTG
Cuts at: 355 1405 1532 355
Size: 1050 127 2239
Fragments arranged by size:
2239 1050 127
MspA1I CmG'CkG
Cuts at: 527 841 1201 2214 2459 527
Size: 314 360 1013 245 1484
Fragments arranged by size:
1484 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1597 1597
Size: 3416
NarI GG'CG_CC
Cuts at: 847 1095 847
Size: 248 3168
NcoI C'CATG_G
Cuts at: 107 107
Size: 3416
NgoMIV G'CCGG_C
Cuts at: 1595 1595
Size: 3416
NspI r_CATG'y
Cuts at: 469 969 1876 469
Size: 500 907 2009
Fragments arranged by size:
2009 907 500
PciI A'CATG_T
Cuts at: 1872 1872
Size: 3416
PmeI GTTT'AAAC
Cuts at: 415 415
Size: 3416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2555 2587 2555
Size: 32 3384
PstI C_TGCA'G
Cuts at: 463 1148 463
Size: 685 2731
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3416
PvuII CAG'CTG
Cuts at: 841 1201 841
Size: 360 3056
RsrII CG'GwC_CG
Cuts at: 1611 1611
Size: 3416
SacI G_AGCT'C
Cuts at: 430 430
Size: 3416
SalI G'TCGA_C
Cuts at: 453 453
Size: 3416
SapI GCTCTTCn'nnn_
Cuts at: 1439 1649 1439
Size: 210 3206
SbfI CC_TGCA'GG
Cuts at: 463 463
Size: 3416
ScaI AGT'ACT
Cuts at: 163 331 2933 163
Size: 168 2602 646
Fragments arranged by size:
2602 646 168
SfcI C'TryA_G
Cuts at: 33 459 1144 2137 2328 33
Size: 426 685 993 191 1121
Fragments arranged by size:
1121 993 685 426 191
SfoI GGC'GCC
Cuts at: 848 1096 848
Size: 248 3168
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3416
SmaI CCC'GGG
Cuts at: 226 438 226
Size: 212 3204
SmlI C'TyrA_G
Cuts at: 571 1486 1978 2240 571
Size: 915 492 262 1747
Fragments arranged by size:
1747 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3416
SphI G_CATG'C
Cuts at: 469 969 469
Size: 500 2916
StyI C'CwwG_G
Cuts at: 107 550 904 107
Size: 443 354 2619
Fragments arranged by size:
2619 443 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 999 1132 1591 1800 999
Size: 133 459 209 2615
Fragments arranged by size:
2615 459 209 133
TatI w'GTAC_w
Cuts at: 161 329 2931 161
Size: 168 2602 646
Fragments arranged by size:
2602 646 168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2933 2965 2933
Size: 32 3384
Tth111I GACn'n_nGTC
Cuts at: 1213 1213
Size: 3416
XbaI T'CTAG_A
Cuts at: 447 447
Size: 3416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 911 911
Size: 3416
XmaI C'CCGG_G
Cuts at: 224 436 224
Size: 212 3204
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI