Restriction map for
pF1KB8869
(Circular) (Six-base) MAPSORT of: pF1KB8869.seq Check: 7535 from: 1 to: 3512
pF1KB8869 3512 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 550 550
Size: 3512
Acc65I G'GTAC_C
Cuts at: 370 528 370
Size: 158 3354
AclI AA'CG_TT
Cuts at: 3352 3352
Size: 3512
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 317 1337 1769 2516 2784 185
Size: 132 1020 432 747 268 913
Fragments arranged by size:
1020 913 747 432 268 132
AflIII A'CryG_T
Cuts at: 432 864 1968 2945 432
Size: 432 1104 977 999
Fragments arranged by size:
1104 999 977 432
AgeI A'CCGG_T
Cuts at: 1900 1900
Size: 3512
AlwNI CAG_nnn'CTG
Cuts at: 126 817 2384 126
Size: 691 1567 1254
Fragments arranged by size:
1567 1254 691
ApaLI G'TGCA_C
Cuts at: 2282 2282
Size: 3512
ApoI r'AATT_y
Cuts at: 264 304 516 2865 3121 264
Size: 40 212 2349 256 655
Fragments arranged by size:
2349 655 256 212 40
AseI AT'TA_AT
Cuts at: 20 347 20
Size: 327 3185
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3512
AvaI C'yCGr_G
Cuts at: 365 532 365
Size: 167 3345
AvrII C'CTAG_G
Cuts at: 258 258
Size: 3512
BamHI G'GATC_C
Cuts at: 537 537
Size: 3512
BanI G'GyrC_C
Cuts at: 370 528 942 1190 1225 370
Size: 158 414 248 35 2657
Fragments arranged by size:
2657 414 248 158 35
BanII G_rGCy'C
Cuts at: 416 526 1556 416
Size: 110 1030 2372
Fragments arranged by size:
2372 1030 110
BbeI G_GCGC'C
Cuts at: 946 1194 946
Size: 248 3264
BbsI GAAGACnn'nnnn_
Cuts at: 157 184 254 157
Size: 27 70 3415
Fragments arranged by size:
3415 70 27
BciVI GTATCCnnnnn_n'
Cuts at: 1410 2171 3512 1410
Size: 761 1341 1410
Fragments arranged by size:
1410 1341 761
BclI T'GATC_A
Cuts at: 281 1032 281
Size: 751 2761
BglII A'GATC_T
Cuts at: 1027 3064 1027
Size: 2037 1475
BlpI GC'TnA_GC
Cuts at: 624 624
Size: 3512
Bme1580I G_kGCm'C
Cuts at: 1137 1230 2286 1137
Size: 93 1056 2363
Fragments arranged by size:
2363 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 724 805 1140 3284 724
Size: 81 335 2144 952
Fragments arranged by size:
2144 952 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1879 1879
Size: 3512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 148
Size: 3512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1567 2059 2357 688
Size: 879 492 298 1843
Fragments arranged by size:
1843 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3216 3216
Size: 3512
BsaAI yAC'GTr
Cuts at: 865 865
Size: 3512
BsaBI GATnn'nnATC
Cuts at: 6 1026 3063 6
Size: 1020 2037 455
Fragments arranged by size:
2037 1020 455
BsaHI Gr'CG_yC
Cuts at: 943 1191 1893 3389 943
Size: 248 702 1496 1066
Fragments arranged by size:
1496 1066 702 248
BsaWI w'CCGG_w
Cuts at: 701 1222 1900 2174 2321 2732 701
Size: 521 678 274 147 411 1481
Fragments arranged by size:
1481 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 937 1695 2272 2768 937
Size: 758 577 496 1681
Fragments arranged by size:
1681 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1054 1100 2308 104
Size: 950 46 1208 1308
Fragments arranged by size:
1308 1208 950 46
BsiHKAI G_wGCw'C
Cuts at: 416 526 1304 1494 2286 416
Size: 110 778 190 792 1642
Fragments arranged by size:
1642 792 778 190 110
BsmI GAATG_Cn'
Cuts at: 356 356
Size: 3512
Bsp1286I G_dGCh'C
Cuts at: 416 526 1137 1230 1304 1494 1556 2286 416
Size: 110 611 93 74 190 62 730 1642
Fragments arranged by size:
1642 730 611 190 110 93 74 62
BspEI T'CCGG_A
Cuts at: 2732 2732
Size: 3512
BspHI T'CATG_A
Cuts at: 284 416 2688 284
Size: 132 2272 1108
Fragments arranged by size:
2272 1108 132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 562 776 1078 1459 562
Size: 214 302 381 2615
Fragments arranged by size:
2615 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1804 1858 3342 3507 50
Size: 1754 54 1484 165 55
Fragments arranged by size:
1754 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1424 1424
Size: 3512
BsrFI r'CCGG_y
Cuts at: 1510 1691 1900 1510
Size: 181 209 3122
Fragments arranged by size:
3122 209 181
BssSI C'ACGA_G
Cuts at: 241 1783 2141 241
Size: 1542 358 1612
Fragments arranged by size:
1612 1542 358
BstAPI GCAn_nnn'nTGC
Cuts at: 889 889
Size: 3512
BstBI TT'CG_AA
Cuts at: 1873 1873
Size: 3512
BstXI CCAn_nnnn'nTGG
Cuts at: 205 205
Size: 3512
BstYI r'GATC_y
Cuts at: 1 537 1004 1027 1362 1608 2620 2706 2718
Size: 536 467 23 335 246 1012 86 12
Cuts at: 2718 3064 1
Size: 346 449
Fragments arranged by size:
1012 536 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3512
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1462 1643 1806 1462
Size: 181 163 3168
Fragments arranged by size:
3168 181 163
BtsI GCAGTG_nn'
Cuts at: 755 755
Size: 3512
DraI TTT'AAA
Cuts at: 511 511
Size: 3512
DraIII CAC_nnn'GTG
Cuts at: 225 225
Size: 3512
DrdI GACnn_nn'nnGTC
Cuts at: 1218 2076 1218
Size: 858 2654
EaeI y'GGCC_r
Cuts at: 1097 1271 1662 1689 3034 1097
Size: 174 391 27 1345 1575
Fragments arranged by size:
1575 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1097 1097
Size: 3512
EarI CTCTTCn'nnn_
Cuts at: 107 1535 1745 107
Size: 1428 210 1874
Fragments arranged by size:
1874 1428 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1720 2030 2176 3322 1720
Size: 310 146 1146 1910
Fragments arranged by size:
1910 1146 310 146
EcoICRI GAG'CTC
Cuts at: 414 524 414
Size: 110 3402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 185 317 1337 1769 2516 2784 148
Size: 37 132 1020 432 747 268 876
Fragments arranged by size:
1020 876 747 432 268 132 37
EcoO109I rG'GnC_Cy
Cuts at: 651 651
Size: 3512
EcoRI G'AATT_C
Cuts at: 516 516
Size: 3512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1531 1531
Size: 3512
FspI TGC'GCA
Cuts at: 1293 1293
Size: 3512
HaeII r_GCGC'y
Cuts at: 946 1194 2216 3195 946
Size: 248 1022 979 1263
Fragments arranged by size:
1263 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1354 1384 1386 1416 1354
Size: 30 2 30 3450
Fragments arranged by size:
3450 30 30 2
HincII GTy'rAC
Cuts at: 551 551
Size: 3512
KasI G'GCGC_C
Cuts at: 942 1190 942
Size: 248 3264
KpnI G_GTAC'C
Cuts at: 374 532 374
Size: 158 3354
MluI A'CGCG_T
Cuts at: 2945 2945
Size: 3512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2183 2367 2183
Size: 184 3328
MscI TGG'CCA
Cuts at: 1273 1273
Size: 3512
MslI CAynn'nnrTG
Cuts at: 283 1501 1628 283
Size: 1218 127 2167
Fragments arranged by size:
2167 1218 127
MspA1I CmG'CkG
Cuts at: 205 382 623 937 1297 2310 2555 205
Size: 177 241 314 360 1013 245 1162
Fragments arranged by size:
1162 1013 360 314 245 241 177
NaeI GCC'GGC
Cuts at: 1693 1693
Size: 3512
NarI GG'CG_CC
Cuts at: 943 1191 943
Size: 248 3264
NcoI C'CATG_G
Cuts at: 107 107
Size: 3512
NgoMIV G'CCGG_C
Cuts at: 1691 1691
Size: 3512
NspI r_CATG'y
Cuts at: 436 565 1065 1972 436
Size: 129 500 907 1976
Fragments arranged by size:
1976 907 500 129
PciI A'CATG_T
Cuts at: 432 1968 432
Size: 1536 1976
PmeI GTTT'AAAC
Cuts at: 511 511
Size: 3512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2651 2683 2651
Size: 32 3480
PspXI vC'TCGA_Gb
Cuts at: 365 365
Size: 3512
PstI C_TGCA'G
Cuts at: 559 1244 559
Size: 685 2827
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3512
PvuII CAG'CTG
Cuts at: 205 382 937 1297 205
Size: 177 555 360 2420
Fragments arranged by size:
2420 555 360 177
RsrII CG'GwC_CG
Cuts at: 1707 1707
Size: 3512
SacI G_AGCT'C
Cuts at: 416 526 416
Size: 110 3402
SalI G'TCGA_C
Cuts at: 549 549
Size: 3512
SapI GCTCTTCn'nnn_
Cuts at: 1535 1745 1535
Size: 210 3302
SbfI CC_TGCA'GG
Cuts at: 559 559
Size: 3512
ScaI AGT'ACT
Cuts at: 3029 3029
Size: 3512
SfcI C'TryA_G
Cuts at: 33 555 1240 2233 2424 33
Size: 522 685 993 191 1121
Fragments arranged by size:
1121 993 685 522 191
SfoI GGC'GCC
Cuts at: 944 1192 944
Size: 248 3264
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3512
SmaI CCC'GGG
Cuts at: 534 534
Size: 3512
SmlI C'TyrA_G
Cuts at: 365 667 1582 2074 2336 365
Size: 302 915 492 262 1541
Fragments arranged by size:
1541 915 492 302 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3512
SphI G_CATG'C
Cuts at: 565 1065 565
Size: 500 3012
StuI AGG'CCT
Cuts at: 404 404
Size: 3512
StyI C'CwwG_G
Cuts at: 107 258 646 1000 107
Size: 151 388 354 2619
Fragments arranged by size:
2619 388 354 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1095 1228 1687 1896 1095
Size: 133 459 209 2711
Fragments arranged by size:
2711 459 209 133
TatI w'GTAC_w
Cuts at: 3027 3027
Size: 3512
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 3480
Tth111I GACn'n_nGTC
Cuts at: 1309 1309
Size: 3512
XbaI T'CTAG_A
Cuts at: 543 543
Size: 3512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1007 1007
Size: 3512
XhoI C'TCGA_G
Cuts at: 365 365
Size: 3512
XmaI C'CCGG_G
Cuts at: 532 532
Size: 3512
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XmnI ZraI