Restriction map for
pF1KB8871
(Circular) (Six-base) MAPSORT of: pF1KB8871.seq Check: 4329 from: 1 to: 3587
pF1KB8871 3587 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 523 523
Size: 3587
AccI GT'mk_AC
Cuts at: 625 625
Size: 3587
Acc65I G'GTAC_C
Cuts at: 603 603
Size: 3587
AclI AA'CG_TT
Cuts at: 3427 3427
Size: 3587
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 1412 1844 2591 2859 204
Size: 1208 432 747 268 932
Fragments arranged by size:
1208 932 747 432 268
AflII C'TTAA_G
Cuts at: 211 211
Size: 3587
AflIII A'CryG_T
Cuts at: 939 2043 3020 939
Size: 1104 977 1506
Fragments arranged by size:
1506 1104 977
AgeI A'CCGG_T
Cuts at: 1975 1975
Size: 3587
AlwNI CAG_nnn'CTG
Cuts at: 393 892 2459 393
Size: 499 1567 1521
Fragments arranged by size:
1567 1521 499
ApaLI G'TGCA_C
Cuts at: 2357 2357
Size: 3587
ApoI r'AATT_y
Cuts at: 591 2940 3196 591
Size: 2349 256 982
Fragments arranged by size:
2349 982 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3587
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3587
AvaI C'yCGr_G
Cuts at: 185 607 185
Size: 422 3165
BamHI G'GATC_C
Cuts at: 612 612
Size: 3587
BanI G'GyrC_C
Cuts at: 603 1017 1265 1300 603
Size: 414 248 35 2890
Fragments arranged by size:
2890 414 248 35
BanII G_rGCy'C
Cuts at: 295 509 601 1631 295
Size: 214 92 1030 2251
Fragments arranged by size:
2251 1030 214 92
BbeI G_GCGC'C
Cuts at: 1021 1269 1021
Size: 248 3339
BbsI GAAGACnn'nnnn_
Cuts at: 210 210
Size: 3587
BciVI GTATCCnnnnn_n'
Cuts at: 1485 2246 3587 1485
Size: 761 1341 1485
Fragments arranged by size:
1485 1341 761
BclI T'GATC_A
Cuts at: 1107 1107
Size: 3587
BglI GCCn_nnn'nGGC
Cuts at: 490 490
Size: 3587
BglII A'GATC_T
Cuts at: 1102 3139 1102
Size: 2037 1550
BlpI GC'TnA_GC
Cuts at: 699 699
Size: 3587
Bme1580I G_kGCm'C
Cuts at: 1212 1305 2361 1212
Size: 93 1056 2438
Fragments arranged by size:
2438 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 799 880 1215 3359 799
Size: 81 335 2144 1027
Fragments arranged by size:
2144 1027 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 474 1954 474
Size: 1480 2107
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 217 561 153
Size: 64 344 3179
Fragments arranged by size:
3179 344 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1642 2134 2432 763
Size: 879 492 298 1918
Fragments arranged by size:
1918 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3291 3291
Size: 3587
BsaAI yAC'GTr
Cuts at: 564 940 564
Size: 376 3211
BsaBI GATnn'nnATC
Cuts at: 6 1101 3138 6
Size: 1095 2037 455
Fragments arranged by size:
2037 1095 455
BsaHI Gr'CG_yC
Cuts at: 1018 1266 1968 3464 1018
Size: 248 702 1496 1141
Fragments arranged by size:
1496 1141 702 248
BsaWI w'CCGG_w
Cuts at: 776 1297 1975 2249 2396 2807 776
Size: 521 678 274 147 411 1556
Fragments arranged by size:
1556 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 493 499 493
Size: 6 3581
BseYI C'CCAG_C
Cuts at: 377 397 534 1012 1770 2347 2843 377
Size: 20 137 478 758 577 496 1121
Fragments arranged by size:
1121 758 577 496 478 137 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 367
Size: 3587
BsiEI CG_ry'CG
Cuts at: 104 1129 1175 2383 104
Size: 1025 46 1208 1308
Fragments arranged by size:
1308 1208 1025 46
BsiHKAI G_wGCw'C
Cuts at: 254 601 1379 1569 2361 254
Size: 347 778 190 792 1480
Fragments arranged by size:
1480 792 778 347 190
BsmI GAATG_Cn'
Cuts at: 308 308
Size: 3587
Bsp1286I G_dGCh'C
Cuts at: 254 295 509 601 1212 1305 1379 1569 1631
Size: 41 214 92 611 93 74 190 62
Cuts at: 1631 2361 254
Size: 730 1480
Fragments arranged by size:
1480 730 611 214 190 93 92 74
62 41
BspEI T'CCGG_A
Cuts at: 2807 2807
Size: 3587
BspHI T'CATG_A
Cuts at: 2763 2763
Size: 3587
BspMI ACCTGCnnnn'nnnn_
Cuts at: 523 637 851 1153 1534 523
Size: 114 214 302 381 2576
Fragments arranged by size:
2576 381 302 214 114
BsrBI CCG'CTC
Cuts at: 50 1879 1933 3417 3582 50
Size: 1829 54 1484 165 55
Fragments arranged by size:
1829 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1499 1499
Size: 3587
BsrFI r'CCGG_y
Cuts at: 1585 1766 1975 1585
Size: 181 209 3197
Fragments arranged by size:
3197 209 181
BssSI C'ACGA_G
Cuts at: 1858 2216 1858
Size: 358 3229
BstAPI GCAn_nnn'nTGC
Cuts at: 390 964 390
Size: 574 3013
BstBI TT'CG_AA
Cuts at: 1948 1948
Size: 3587
BstYI r'GATC_y
Cuts at: 1 612 1079 1102 1437 1683 2695 2781 2793
Size: 611 467 23 335 246 1012 86 12
Cuts at: 2793 3139 1
Size: 346 449
Fragments arranged by size:
1012 611 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 178 178
Size: 3587
BtgI C'CryG_G
Cuts at: 114 155 114
Size: 41 3546
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1537 1718 1881 1537
Size: 181 163 3243
Fragments arranged by size:
3243 181 163
BtsI GCAGTG_nn'
Cuts at: 830 830
Size: 3587
DraI TTT'AAA
Cuts at: 586 586
Size: 3587
DrdI GACnn_nn'nnGTC
Cuts at: 1293 2151 1293
Size: 858 2729
EaeI y'GGCC_r
Cuts at: 350 491 1172 1346 1737 1764 3109 350
Size: 141 681 174 391 27 1345 828
Fragments arranged by size:
1345 828 681 391 174 141 27
EagI C'GGCC_G
Cuts at: 1172 1172
Size: 3587
EarI CTCTTCn'nnn_
Cuts at: 1610 1820 1610
Size: 210 3377
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1795 2105 2251 3397 1795
Size: 310 146 1146 1985
Fragments arranged by size:
1985 1146 310 146
EcoICRI GAG'CTC
Cuts at: 599 599
Size: 3587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 204 217 561 1412 1844 2591 2859 153
Size: 51 13 344 851 432 747 268 881
Fragments arranged by size:
881 851 747 432 344 268 51 13
EcoO109I rG'GnC_Cy
Cuts at: 182 483 726 182
Size: 301 243 3043
Fragments arranged by size:
3043 301 243
EcoRI G'AATT_C
Cuts at: 591 591
Size: 3587
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1606 1606
Size: 3587
FspI TGC'GCA
Cuts at: 1368 1368
Size: 3587
HaeII r_GCGC'y
Cuts at: 232 1021 1269 2291 3270 232
Size: 789 248 1022 979 549
Fragments arranged by size:
1022 979 789 549 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1429 1459 1461 1491 1429
Size: 30 2 30 3525
Fragments arranged by size:
3525 30 30 2
HincII GTy'rAC
Cuts at: 626 626
Size: 3587
KasI G'GCGC_C
Cuts at: 1017 1265 1017
Size: 248 3339
KpnI G_GTAC'C
Cuts at: 607 607
Size: 3587
MluI A'CGCG_T
Cuts at: 3020 3020
Size: 3587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2258 2442 2258
Size: 184 3403
MscI TGG'CCA
Cuts at: 352 1348 352
Size: 996 2591
MslI CAynn'nnrTG
Cuts at: 1576 1703 1576
Size: 127 3460
MspA1I CmG'CkG
Cuts at: 224 305 381 390 698 1012 1372 2385 2630
Size: 81 76 9 308 314 360 1013 245
Cuts at: 2630 224
Size: 1181
Fragments arranged by size:
1181 1013 360 314 308 245 81 76
9
NaeI GCC'GGC
Cuts at: 1768 1768
Size: 3587
NarI GG'CG_CC
Cuts at: 1018 1266 1018
Size: 248 3339
NgoMIV G'CCGG_C
Cuts at: 1766 1766
Size: 3587
NspI r_CATG'y
Cuts at: 135 640 1140 2047 135
Size: 505 500 907 1675
Fragments arranged by size:
1675 907 505 500
PciI A'CATG_T
Cuts at: 2043 2043
Size: 3587
PflMI CCAn_nnn'nTGG
Cuts at: 327 327
Size: 3587
PfoI T'CCnGG_A
Cuts at: 137 233 137
Size: 96 3491
PmeI GTTT'AAAC
Cuts at: 586 586
Size: 3587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2726 2758 2726
Size: 32 3555
PpuMI rG'GwC_Cy
Cuts at: 182 182
Size: 3587
PstI C_TGCA'G
Cuts at: 634 1319 634
Size: 685 2902
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3587
PvuII CAG'CTG
Cuts at: 381 390 1012 1372 381
Size: 9 622 360 2596
Fragments arranged by size:
2596 622 360 9
RsrII CG'GwC_CG
Cuts at: 1782 1782
Size: 3587
SacI G_AGCT'C
Cuts at: 601 601
Size: 3587
SalI G'TCGA_C
Cuts at: 624 624
Size: 3587
SapI GCTCTTCn'nnn_
Cuts at: 1610 1820 1610
Size: 210 3377
SbfI CC_TGCA'GG
Cuts at: 634 634
Size: 3587
ScaI AGT'ACT
Cuts at: 3104 3104
Size: 3587
SfcI C'TryA_G
Cuts at: 33 630 1315 2308 2499 33
Size: 597 685 993 191 1121
Fragments arranged by size:
1121 993 685 597 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 490 490
Size: 3587
SfoI GGC'GCC
Cuts at: 1019 1267 1019
Size: 248 3339
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3587
SmaI CCC'GGG
Cuts at: 187 609 187
Size: 422 3165
SmlI C'TyrA_G
Cuts at: 211 742 1657 2149 2411 211
Size: 531 915 492 262 1387
Fragments arranged by size:
1387 915 531 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3587
SphI G_CATG'C
Cuts at: 640 1140 640
Size: 500 3087
StyI C'CwwG_G
Cuts at: 287 721 1075 287
Size: 434 354 2799
Fragments arranged by size:
2799 434 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 405 1170 1303 1762 1971 405
Size: 765 133 459 209 2021
Fragments arranged by size:
2021 765 459 209 133
TatI w'GTAC_w
Cuts at: 3102 3102
Size: 3587
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3104 3136 3104
Size: 32 3555
Tth111I GACn'n_nGTC
Cuts at: 1384 1384
Size: 3587
XbaI T'CTAG_A
Cuts at: 618 618
Size: 3587
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1082 1082
Size: 3587
XmaI C'CCGG_G
Cuts at: 185 607 185
Size: 422 3165
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI