Restriction map for
pF1KB8876
(Circular) (Six-base) MAPSORT of: pF1KB8876.seq Check: 3359 from: 1 to: 3614
pF1KB8876 3614 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 350 652 350
Size: 302 3312
Acc65I G'GTAC_C
Cuts at: 630 630
Size: 3614
AclI AA'CG_TT
Cuts at: 3454 3454
Size: 3614
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 1439 1871 2618 2886 503
Size: 936 432 747 268 1231
Fragments arranged by size:
1231 936 747 432 268
AflIII A'CryG_T
Cuts at: 966 2070 3047 966
Size: 1104 977 1533
Fragments arranged by size:
1533 1104 977
AgeI A'CCGG_T
Cuts at: 280 2002 280
Size: 1722 1892
AleI CACnn'nnGTG
Cuts at: 535 535
Size: 3614
AlwNI CAG_nnn'CTG
Cuts at: 342 919 2486 342
Size: 577 1567 1470
Fragments arranged by size:
1567 1470 577
ApaLI G'TGCA_C
Cuts at: 2384 2384
Size: 3614
ApoI r'AATT_y
Cuts at: 618 2967 3223 618
Size: 2349 256 1009
Fragments arranged by size:
2349 1009 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3614
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3614
AvaI C'yCGr_G
Cuts at: 634 634
Size: 3614
BamHI G'GATC_C
Cuts at: 639 639
Size: 3614
BanI G'GyrC_C
Cuts at: 630 1044 1292 1327 630
Size: 414 248 35 2917
Fragments arranged by size:
2917 414 248 35
BanII G_rGCy'C
Cuts at: 305 628 1658 305
Size: 323 1030 2261
Fragments arranged by size:
2261 1030 323
BbeI G_GCGC'C
Cuts at: 1048 1296 1048
Size: 248 3366
BbvCI CC'TCA_GC
Cuts at: 595 595
Size: 3614
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3580
BciVI GTATCCnnnnn_n'
Cuts at: 1512 2273 3614 1512
Size: 761 1341 1512
Fragments arranged by size:
1512 1341 761
BclI T'GATC_A
Cuts at: 1134 1134
Size: 3614
BfrBI ATG'CAT
Cuts at: 111 111
Size: 3614
BglII A'GATC_T
Cuts at: 1129 3166 1129
Size: 2037 1577
BlpI GC'TnA_GC
Cuts at: 726 726
Size: 3614
Bme1580I G_kGCm'C
Cuts at: 176 1239 1332 2388 176
Size: 1063 93 1056 1402
Fragments arranged by size:
1402 1063 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 826 907 1242 3386 826
Size: 81 335 2144 1054
Fragments arranged by size:
2144 1054 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1981 1981
Size: 3614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 502 430
Size: 72 3542
Bpu10I CC'TnA_GC
Cuts at: 547 595 547
Size: 48 3566
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 1669 2161 2459 790
Size: 879 492 298 1945
Fragments arranged by size:
1945 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 478 569 3318 478
Size: 91 2749 774
Fragments arranged by size:
2749 774 91
BsaAI yAC'GTr
Cuts at: 435 967 435
Size: 532 3082
BsaBI GATnn'nnATC
Cuts at: 6 1128 3165 6
Size: 1122 2037 455
Fragments arranged by size:
2037 1122 455
BsaHI Gr'CG_yC
Cuts at: 1045 1293 1995 3491 1045
Size: 248 702 1496 1168
Fragments arranged by size:
1496 1168 702 248
BsaWI w'CCGG_w
Cuts at: 280 803 1324 2002 2276 2423 2834 280
Size: 523 521 678 274 147 411 1060
Fragments arranged by size:
1060 678 523 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 252 252
Size: 3614
BseYI C'CCAG_C
Cuts at: 1039 1797 2374 2870 1039
Size: 758 577 496 1783
Fragments arranged by size:
1783 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1156 1202 2410 104
Size: 1052 46 1208 1308
Fragments arranged by size:
1308 1208 1052 46
BsiHKAI G_wGCw'C
Cuts at: 628 1406 1596 2388 628
Size: 778 190 792 1854
Fragments arranged by size:
1854 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 176 305 628 1239 1332 1406 1596 1658 2388
Size: 129 323 611 93 74 190 62 730
Cuts at: 2388 176
Size: 1402
Fragments arranged by size:
1402 730 611 323 190 129 93 74
62
BspEI T'CCGG_A
Cuts at: 2834 2834
Size: 3614
BspHI T'CATG_A
Cuts at: 2790 2790
Size: 3614
BspMI ACCTGCnnnn'nnnn_
Cuts at: 664 878 1180 1561 664
Size: 214 302 381 2717
Fragments arranged by size:
2717 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1906 1960 3444 3609 50
Size: 1856 54 1484 165 55
Fragments arranged by size:
1856 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1526 1526
Size: 3614
BsrFI r'CCGG_y
Cuts at: 280 1612 1793 2002 280
Size: 1332 181 209 1892
Fragments arranged by size:
1892 1332 209 181
BssSI C'ACGA_G
Cuts at: 1885 2243 1885
Size: 358 3256
BstAPI GCAn_nnn'nTGC
Cuts at: 576 991 576
Size: 415 3199
BstBI TT'CG_AA
Cuts at: 1975 1975
Size: 3614
BstXI CCAn_nnnn'nTGG
Cuts at: 408 480 537 408
Size: 72 57 3485
Fragments arranged by size:
3485 72 57
BstYI r'GATC_y
Cuts at: 1 359 423 639 1106 1129 1464 1710 2722
Size: 358 64 216 467 23 335 246 1012
Cuts at: 2722 2808 2820 3166 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 358 346 335 246 216
86 64 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1564 1745 1908 1564
Size: 181 163 3270
Fragments arranged by size:
3270 181 163
BtsI GCAGTG_nn'
Cuts at: 857 857
Size: 3614
DraI TTT'AAA
Cuts at: 613 613
Size: 3614
DrdI GACnn_nn'nnGTC
Cuts at: 1320 2178 1320
Size: 858 2756
EaeI y'GGCC_r
Cuts at: 1199 1373 1764 1791 3136 1199
Size: 174 391 27 1345 1677
Fragments arranged by size:
1677 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1199 1199
Size: 3614
EarI CTCTTCn'nnn_
Cuts at: 1637 1847 1637
Size: 210 3404
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1822 2132 2278 3424 1822
Size: 310 146 1146 2012
Fragments arranged by size:
2012 1146 310 146
EcoICRI GAG'CTC
Cuts at: 626 626
Size: 3614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 502 503 1439 1871 2618 2886 430
Size: 72 1 936 432 747 268 1158
Fragments arranged by size:
1158 936 747 432 268 72 1
EcoO109I rG'GnC_Cy
Cuts at: 753 753
Size: 3614
EcoRI G'AATT_C
Cuts at: 618 618
Size: 3614
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1633 1633
Size: 3614
FspI TGC'GCA
Cuts at: 1395 1395
Size: 3614
HaeII r_GCGC'y
Cuts at: 1048 1296 2318 3297 1048
Size: 248 1022 979 1365
Fragments arranged by size:
1365 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1456 1486 1488 1518 1456
Size: 30 2 30 3552
Fragments arranged by size:
3552 30 30 2
HincII GTy'rAC
Cuts at: 653 653
Size: 3614
HindIII A'AGCT_T
Cuts at: 515 515
Size: 3614
KasI G'GCGC_C
Cuts at: 1044 1292 1044
Size: 248 3366
KpnI G_GTAC'C
Cuts at: 634 634
Size: 3614
MfeI C'AATT_G
Cuts at: 218 218
Size: 3614
MluI A'CGCG_T
Cuts at: 3047 3047
Size: 3614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 425 2285 2469 425
Size: 1860 184 1570
Fragments arranged by size:
1860 1570 184
MscI TGG'CCA
Cuts at: 1375 1375
Size: 3614
MslI CAynn'nnrTG
Cuts at: 370 535 1603 1730 370
Size: 165 1068 127 2254
Fragments arranged by size:
2254 1068 165 127
MspA1I CmG'CkG
Cuts at: 588 725 1039 1399 2412 2657 588
Size: 137 314 360 1013 245 1545
Fragments arranged by size:
1545 1013 360 314 245 137
NaeI GCC'GGC
Cuts at: 1795 1795
Size: 3614
NarI GG'CG_CC
Cuts at: 1045 1293 1045
Size: 248 3366
NgoMIV G'CCGG_C
Cuts at: 1793 1793
Size: 3614
NsiI A_TGCA'T
Cuts at: 113 113
Size: 3614
NspI r_CATG'y
Cuts at: 579 667 1167 2074 579
Size: 88 500 907 2119
Fragments arranged by size:
2119 907 500 88
PasI CC'CwG_GG
Cuts at: 467 601 467
Size: 134 3480
PciI A'CATG_T
Cuts at: 2070 2070
Size: 3614
PflMI CCAn_nnn'nTGG
Cuts at: 113 342 113
Size: 229 3385
PfoI T'CCnGG_A
Cuts at: 419 509 419
Size: 90 3524
PmeI GTTT'AAAC
Cuts at: 613 613
Size: 3614
PmlI CAC'GTG
Cuts at: 435 435
Size: 3614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2753 2785 2753
Size: 32 3582
PshAI GACnn'nnGTC
Cuts at: 220 220
Size: 3614
PstI C_TGCA'G
Cuts at: 560 572 661 1346 560
Size: 12 89 685 2828
Fragments arranged by size:
2828 685 89 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3614
PvuII CAG'CTG
Cuts at: 588 1039 1399 588
Size: 451 360 2803
Fragments arranged by size:
2803 451 360
RsrII CG'GwC_CG
Cuts at: 1809 1809
Size: 3614
SacI G_AGCT'C
Cuts at: 628 628
Size: 3614
SalI G'TCGA_C
Cuts at: 651 651
Size: 3614
SapI GCTCTTCn'nnn_
Cuts at: 1637 1847 1637
Size: 210 3404
SbfI CC_TGCA'GG
Cuts at: 661 661
Size: 3614
ScaI AGT'ACT
Cuts at: 3131 3131
Size: 3614
SfcI C'TryA_G
Cuts at: 33 556 568 657 1342 2335 2526 33
Size: 523 12 89 685 993 191 1121
Fragments arranged by size:
1121 993 685 523 191 89 12
SfoI GGC'GCC
Cuts at: 1046 1294 1046
Size: 248 3366
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3614
SmaI CCC'GGG
Cuts at: 636 636
Size: 3614
SmlI C'TyrA_G
Cuts at: 769 1684 2176 2438 769
Size: 915 492 262 1945
Fragments arranged by size:
1945 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3614
SphI G_CATG'C
Cuts at: 667 1167 667
Size: 500 3114
StyI C'CwwG_G
Cuts at: 488 748 1102 488
Size: 260 354 3000
Fragments arranged by size:
3000 354 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 593 1197 1330 1789 1998 593
Size: 604 133 459 209 2209
Fragments arranged by size:
2209 604 459 209 133
TatI w'GTAC_w
Cuts at: 3129 3129
Size: 3614
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 565 597 3131 3163 565
Size: 32 2534 32 1016
Fragments arranged by size:
2534 1016 32 32
Tth111I GACn'n_nGTC
Cuts at: 1411 1411
Size: 3614
XbaI T'CTAG_A
Cuts at: 645 645
Size: 3614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 537 1109 537
Size: 572 3042
XmaI C'CCGG_G
Cuts at: 634 634
Size: 3614
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI NcoI NdeI NheI NotI NruI PacI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI