Restriction map for
pF1KB8878
(Circular) (Six-base) MAPSORT of: pF1KB8878.seq Check: 3083 from: 1 to: 3620
pF1KB8878 3620 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AccI GT'mk_AC
Cuts at: 658 658
Size: 3620
Acc65I G'GTAC_C
Cuts at: 214 636 214
Size: 422 3198
AclI AA'CG_TT
Cuts at: 3460 3460
Size: 3620
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 533 1445 1877 2624 2892 182
Size: 351 912 432 747 268 910
Fragments arranged by size:
912 910 747 432 351 268
AflIII A'CryG_T
Cuts at: 972 2076 3053 972
Size: 1104 977 1539
Fragments arranged by size:
1539 1104 977
AgeI A'CCGG_T
Cuts at: 2008 2008
Size: 3620
AlwNI CAG_nnn'CTG
Cuts at: 925 2492 925
Size: 1567 2053
ApaLI G'TGCA_C
Cuts at: 2390 2390
Size: 3620
ApoI r'AATT_y
Cuts at: 624 2973 3229 624
Size: 2349 256 1015
Fragments arranged by size:
2349 1015 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3620
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3620
AvaI C'yCGr_G
Cuts at: 446 640 446
Size: 194 3426
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 206 239 206
Size: 33 3587
BamHI G'GATC_C
Cuts at: 645 645
Size: 3620
BanI G'GyrC_C
Cuts at: 214 636 1050 1298 1333 214
Size: 422 414 248 35 2501
Fragments arranged by size:
2501 422 414 248 35
BanII G_rGCy'C
Cuts at: 346 373 582 634 1664 346
Size: 27 209 52 1030 2302
Fragments arranged by size:
2302 1030 209 52 27
BbeI G_GCGC'C
Cuts at: 1054 1302 1054
Size: 248 3372
BbsI GAAGACnn'nnnn_
Cuts at: 188 404 188
Size: 216 3404
BciVI GTATCCnnnnn_n'
Cuts at: 1518 2279 3620 1518
Size: 761 1341 1518
Fragments arranged by size:
1518 1341 761
BclI T'GATC_A
Cuts at: 1140 1140
Size: 3620
BglII A'GATC_T
Cuts at: 1135 3172 1135
Size: 2037 1583
BlpI GC'TnA_GC
Cuts at: 732 732
Size: 3620
Bme1580I G_kGCm'C
Cuts at: 1245 1338 2394 1245
Size: 93 1056 2471
Fragments arranged by size:
2471 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 832 913 1248 3392 832
Size: 81 335 2144 1060
Fragments arranged by size:
2144 1060 335 81
BmtI G_CTAG'C
Cuts at: 511 511
Size: 3620
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1987 1987
Size: 3620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 338
Size: 3620
Bpu10I CC'TnA_GC
Cuts at: 380 380
Size: 3620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 796 1675 2167 2465 514
Size: 282 879 492 298 1669
Fragments arranged by size:
1669 879 492 298 282
BsaI GGTCTCn'nnnn_
Cuts at: 3324 3324
Size: 3620
BsaAI yAC'GTr
Cuts at: 973 973
Size: 3620
BsaBI GATnn'nnATC
Cuts at: 6 1134 3171 6
Size: 1128 2037 455
Fragments arranged by size:
2037 1128 455
BsaHI Gr'CG_yC
Cuts at: 1051 1299 2001 3497 1051
Size: 248 702 1496 1174
Fragments arranged by size:
1496 1174 702 248
BsaWI w'CCGG_w
Cuts at: 809 1330 2008 2282 2429 2840 809
Size: 521 678 274 147 411 1589
Fragments arranged by size:
1589 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 316 319 406 316
Size: 3 87 3530
Fragments arranged by size:
3530 87 3
BseYI C'CCAG_C
Cuts at: 152 441 1045 1803 2380 2876 152
Size: 289 604 758 577 496 896
Fragments arranged by size:
896 758 604 577 496 289
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 589
Size: 3620
BsiEI CG_ry'CG
Cuts at: 104 1162 1208 2416 104
Size: 1058 46 1208 1308
Fragments arranged by size:
1308 1208 1058 46
BsiHKAI G_wGCw'C
Cuts at: 373 582 634 1412 1602 2394 373
Size: 209 52 778 190 792 1599
Fragments arranged by size:
1599 792 778 209 190 52
Bsp1286I G_dGCh'C
Cuts at: 346 373 582 634 1245 1338 1412 1602 1664
Size: 27 209 52 611 93 74 190 62
Cuts at: 1664 2394 346
Size: 730 1572
Fragments arranged by size:
1572 730 611 209 190 93 74 62
52 27
BspEI T'CCGG_A
Cuts at: 2840 2840
Size: 3620
BspHI T'CATG_A
Cuts at: 2796 2796
Size: 3620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 188 670 884 1186 1567 188
Size: 482 214 302 381 2241
Fragments arranged by size:
2241 482 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1912 1966 3450 3615 50
Size: 1862 54 1484 165 55
Fragments arranged by size:
1862 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 121 1532 121
Size: 1411 2209
BsrFI r'CCGG_y
Cuts at: 1618 1799 2008 1618
Size: 181 209 3230
Fragments arranged by size:
3230 209 181
BssSI C'ACGA_G
Cuts at: 1891 2249 1891
Size: 358 3262
BstAPI GCAn_nnn'nTGC
Cuts at: 997 997
Size: 3620
BstBI TT'CG_AA
Cuts at: 1981 1981
Size: 3620
BstYI r'GATC_y
Cuts at: 1 645 1112 1135 1470 1716 2728 2814 2826
Size: 644 467 23 335 246 1012 86 12
Cuts at: 2826 3172 1
Size: 346 449
Fragments arranged by size:
1012 644 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3620
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1570 1751 1914 1570
Size: 181 163 3276
Fragments arranged by size:
3276 181 163
BtsI GCAGTG_nn'
Cuts at: 863 863
Size: 3620
DraI TTT'AAA
Cuts at: 619 619
Size: 3620
DrdI GACnn_nn'nnGTC
Cuts at: 1326 2184 1326
Size: 858 2762
EaeI y'GGCC_r
Cuts at: 1205 1379 1770 1797 3142 1205
Size: 174 391 27 1345 1683
Fragments arranged by size:
1683 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1205 1205
Size: 3620
EarI CTCTTCn'nnn_
Cuts at: 236 383 509 1643 1853 236
Size: 147 126 1134 210 2003
Fragments arranged by size:
2003 1134 210 147 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1828 2138 2284 3430 1828
Size: 310 146 1146 2018
Fragments arranged by size:
2018 1146 310 146
EcoICRI GAG'CTC
Cuts at: 371 580 632 371
Size: 209 52 3359
Fragments arranged by size:
3359 209 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 338 533 1445 1877 2624 2892 182
Size: 156 195 912 432 747 268 910
Fragments arranged by size:
912 910 747 432 268 195 156
EcoNI CCTnn'n_nnAGG
Cuts at: 184 379 184
Size: 195 3425
EcoO109I rG'GnC_Cy
Cuts at: 177 189 759 177
Size: 12 570 3038
Fragments arranged by size:
3038 570 12
EcoRI G'AATT_C
Cuts at: 624 624
Size: 3620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1639 1639
Size: 3620
FspI TGC'GCA
Cuts at: 1401 1401
Size: 3620
HaeII r_GCGC'y
Cuts at: 549 1054 1302 2324 3303 549
Size: 505 248 1022 979 866
Fragments arranged by size:
1022 979 866 505 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 235 267 1462 1492 1494 1524 178
Size: 32 25 32 1195 30 2 30 2274
Fragments arranged by size:
2274 1195 32 32 30 30 25 2
HincII GTy'rAC
Cuts at: 659 659
Size: 3620
KasI G'GCGC_C
Cuts at: 1050 1298 1050
Size: 248 3372
KpnI G_GTAC'C
Cuts at: 218 640 218
Size: 422 3198
MluI A'CGCG_T
Cuts at: 3053 3053
Size: 3620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2291 2475 2291
Size: 184 3436
MscI TGG'CCA
Cuts at: 1381 1381
Size: 3620
MslI CAynn'nnrTG
Cuts at: 1609 1736 1609
Size: 127 3493
MspA1I CmG'CkG
Cuts at: 115 152 731 1045 1405 2418 2663 115
Size: 37 579 314 360 1013 245 1072
Fragments arranged by size:
1072 1013 579 360 314 245 37
NaeI GCC'GGC
Cuts at: 1801 1801
Size: 3620
NarI GG'CG_CC
Cuts at: 1051 1299 1051
Size: 248 3372
NcoI C'CATG_G
Cuts at: 107 107
Size: 3620
NgoMIV G'CCGG_C
Cuts at: 1799 1799
Size: 3620
NheI G'CTAG_C
Cuts at: 507 507
Size: 3620
NspI r_CATG'y
Cuts at: 673 1173 2080 673
Size: 500 907 2213
Fragments arranged by size:
2213 907 500
PciI A'CATG_T
Cuts at: 2076 2076
Size: 3620
PfoI T'CCnGG_A
Cuts at: 231 231
Size: 3620
PmeI GTTT'AAAC
Cuts at: 619 619
Size: 3620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2759 2791 2759
Size: 32 3588
PpuMI rG'GwC_Cy
Cuts at: 177 189 177
Size: 12 3608
PstI C_TGCA'G
Cuts at: 667 1352 667
Size: 685 2935
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3620
PvuII CAG'CTG
Cuts at: 115 152 1045 1405 115
Size: 37 893 360 2330
Fragments arranged by size:
2330 893 360 37
RsrII CG'GwC_CG
Cuts at: 1815 1815
Size: 3620
SacI G_AGCT'C
Cuts at: 373 582 634 373
Size: 209 52 3359
Fragments arranged by size:
3359 209 52
SalI G'TCGA_C
Cuts at: 657 657
Size: 3620
SapI GCTCTTCn'nnn_
Cuts at: 1643 1853 1643
Size: 210 3410
SbfI CC_TGCA'GG
Cuts at: 667 667
Size: 3620
ScaI AGT'ACT
Cuts at: 3137 3137
Size: 3620
SfcI C'TryA_G
Cuts at: 33 663 1348 2341 2532 33
Size: 630 685 993 191 1121
Fragments arranged by size:
1121 993 685 630 191
SfoI GGC'GCC
Cuts at: 1052 1300 1052
Size: 248 3372
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3620
SmaI CCC'GGG
Cuts at: 448 642 448
Size: 194 3426
SmlI C'TyrA_G
Cuts at: 529 775 1690 2182 2444 529
Size: 246 915 492 262 1705
Fragments arranged by size:
1705 915 492 262 246
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3620
SphI G_CATG'C
Cuts at: 673 1173 673
Size: 500 3120
SrfI GCCC'GGGC
Cuts at: 448 448
Size: 3620
StyI C'CwwG_G
Cuts at: 107 402 754 1108 107
Size: 295 352 354 2619
Fragments arranged by size:
2619 354 352 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 605 1203 1336 1795 2004 605
Size: 598 133 459 209 2221
Fragments arranged by size:
2221 598 459 209 133
TatI w'GTAC_w
Cuts at: 3135 3135
Size: 3620
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 414 446 3137 3169 414
Size: 32 2691 32 865
Fragments arranged by size:
2691 865 32 32
Tth111I GACn'n_nGTC
Cuts at: 144 1417 144
Size: 1273 2347
XbaI T'CTAG_A
Cuts at: 651 651
Size: 3620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 156 1115 156
Size: 959 2661
XmaI C'CCGG_G
Cuts at: 446 640 446
Size: 194 3426
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BfrBI BglI BmgBI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SspI StuI
SwaI XhoI XmnI ZraI