Restriction map for
pF1KB8886
(Circular) (Six-base) MAPSORT of: pF1KB8886.seq Check: 5044 from: 1 to: 3692
pF1KB8886 3692 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 730 730
Size: 3692
Acc65I G'GTAC_C
Cuts at: 708 708
Size: 3692
AclI AA'CG_TT
Cuts at: 3532 3532
Size: 3692
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 1517 1949 2696 2964 540
Size: 977 432 747 268 1268
Fragments arranged by size:
1268 977 747 432 268
AflIII A'CryG_T
Cuts at: 1044 2148 3125 1044
Size: 1104 977 1611
Fragments arranged by size:
1611 1104 977
AgeI A'CCGG_T
Cuts at: 2080 2080
Size: 3692
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 286 318 286
Size: 32 3660
AlwNI CAG_nnn'CTG
Cuts at: 997 2564 997
Size: 1567 2125
ApaLI G'TGCA_C
Cuts at: 2462 2462
Size: 3692
ApoI r'AATT_y
Cuts at: 273 696 3045 3301 273
Size: 423 2349 256 664
Fragments arranged by size:
2349 664 423 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3692
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
AvaI C'yCGr_G
Cuts at: 712 712
Size: 3692
BamHI G'GATC_C
Cuts at: 717 717
Size: 3692
BanI G'GyrC_C
Cuts at: 708 1122 1370 1405 708
Size: 414 248 35 2995
Fragments arranged by size:
2995 414 248 35
BanII G_rGCy'C
Cuts at: 407 706 1736 407
Size: 299 1030 2363
Fragments arranged by size:
2363 1030 299
BbeI G_GCGC'C
Cuts at: 1126 1374 1126
Size: 248 3444
BbvCI CC'TCA_GC
Cuts at: 616 616
Size: 3692
BciVI GTATCCnnnnn_n'
Cuts at: 1590 2351 3692 1590
Size: 761 1341 1590
Fragments arranged by size:
1590 1341 761
BclI T'GATC_A
Cuts at: 1212 1212
Size: 3692
BglII A'GATC_T
Cuts at: 1207 3244 1207
Size: 2037 1655
BlpI GC'TnA_GC
Cuts at: 804 804
Size: 3692
Bme1580I G_kGCm'C
Cuts at: 1317 1410 2466 1317
Size: 93 1056 2543
Fragments arranged by size:
2543 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 219 904 985 1320 3464 219
Size: 685 81 335 2144 447
Fragments arranged by size:
2144 685 447 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 389 558 2059 389
Size: 169 1501 2022
Fragments arranged by size:
2022 1501 169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 310
Size: 3692
Bpu10I CC'TnA_GC
Cuts at: 616 621 616
Size: 5 3687
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 868 1747 2239 2537 359
Size: 509 879 492 298 1514
Fragments arranged by size:
1514 879 509 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3396 3396
Size: 3692
BsaAI yAC'GTr
Cuts at: 435 1045 435
Size: 610 3082
BsaBI GATnn'nnATC
Cuts at: 6 1206 3243 6
Size: 1200 2037 455
Fragments arranged by size:
2037 1200 455
BsaHI Gr'CG_yC
Cuts at: 1123 1371 2073 3569 1123
Size: 248 702 1496 1246
Fragments arranged by size:
1496 1246 702 248
BsaWI w'CCGG_w
Cuts at: 881 1402 2080 2354 2501 2912 881
Size: 521 678 274 147 411 1661
Fragments arranged by size:
1661 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 579 579
Size: 3692
BseYI C'CCAG_C
Cuts at: 1117 1875 2452 2948 1117
Size: 758 577 496 1861
Fragments arranged by size:
1861 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1234 1280 2488 104
Size: 1130 46 1208 1308
Fragments arranged by size:
1308 1208 1130 46
BsiHKAI G_wGCw'C
Cuts at: 706 1484 1674 2466 706
Size: 778 190 792 1932
Fragments arranged by size:
1932 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 407 706 1317 1410 1484 1674 1736 2466 407
Size: 299 611 93 74 190 62 730 1633
Fragments arranged by size:
1633 730 611 299 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2912 2912
Size: 3692
BspHI T'CATG_A
Cuts at: 2868 2868
Size: 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 742 956 1258 1639 742
Size: 214 302 381 2795
Fragments arranged by size:
2795 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1984 2038 3522 3687 50
Size: 1934 54 1484 165 55
Fragments arranged by size:
1934 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1604 1604
Size: 3692
BsrFI r'CCGG_y
Cuts at: 1690 1871 2080 1690
Size: 181 209 3302
Fragments arranged by size:
3302 209 181
BssSI C'ACGA_G
Cuts at: 1963 2321 1963
Size: 358 3334
BstAPI GCAn_nnn'nTGC
Cuts at: 1069 1069
Size: 3692
BstBI TT'CG_AA
Cuts at: 2053 2053
Size: 3692
BstYI r'GATC_y
Cuts at: 1 717 1184 1207 1542 1788 2800 2886 2898
Size: 716 467 23 335 246 1012 86 12
Cuts at: 2898 3244 1
Size: 346 449
Fragments arranged by size:
1012 716 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3692
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1642 1823 1986 1642
Size: 181 163 3348
Fragments arranged by size:
3348 181 163
BtsI GCAGTG_nn'
Cuts at: 935 935
Size: 3692
DraI TTT'AAA
Cuts at: 691 691
Size: 3692
DrdI GACnn_nn'nnGTC
Cuts at: 1398 2256 1398
Size: 858 2834
EaeI y'GGCC_r
Cuts at: 143 1277 1451 1842 1869 3214 143
Size: 1134 174 391 27 1345 621
Fragments arranged by size:
1345 1134 621 391 174 27
EagI C'GGCC_G
Cuts at: 1277 1277
Size: 3692
EarI CTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1900 2210 2356 3502 1900
Size: 310 146 1146 2090
Fragments arranged by size:
2090 1146 310 146
EcoICRI GAG'CTC
Cuts at: 704 704
Size: 3692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 540 1517 1949 2696 2964 310
Size: 230 977 432 747 268 1038
Fragments arranged by size:
1038 977 747 432 268 230
EcoNI CCTnn'n_nnAGG
Cuts at: 241 241
Size: 3692
EcoO109I rG'GnC_Cy
Cuts at: 339 831 339
Size: 492 3200
EcoRI G'AATT_C
Cuts at: 696 696
Size: 3692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1711 1711
Size: 3692
FspI TGC'GCA
Cuts at: 1473 1473
Size: 3692
HaeII r_GCGC'y
Cuts at: 1126 1374 2396 3375 1126
Size: 248 1022 979 1443
Fragments arranged by size:
1443 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1534 1564 1566 1596 1534
Size: 30 2 30 3630
Fragments arranged by size:
3630 30 30 2
HincII GTy'rAC
Cuts at: 731 731
Size: 3692
KasI G'GCGC_C
Cuts at: 1122 1370 1122
Size: 248 3444
KpnI G_GTAC'C
Cuts at: 712 712
Size: 3692
MluI A'CGCG_T
Cuts at: 3125 3125
Size: 3692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2363 2547 2363
Size: 184 3508
MscI TGG'CCA
Cuts at: 1453 1453
Size: 3692
MslI CAynn'nnrTG
Cuts at: 1681 1808 1681
Size: 127 3565
MspA1I CmG'CkG
Cuts at: 803 1117 1477 2490 2735 803
Size: 314 360 1013 245 1760
Fragments arranged by size:
1760 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1873 1873
Size: 3692
NarI GG'CG_CC
Cuts at: 1123 1371 1123
Size: 248 3444
NcoI C'CATG_G
Cuts at: 107 107
Size: 3692
NgoMIV G'CCGG_C
Cuts at: 1871 1871
Size: 3692
NspI r_CATG'y
Cuts at: 745 1245 2152 745
Size: 500 907 2285
Fragments arranged by size:
2285 907 500
PciI A'CATG_T
Cuts at: 2148 2148
Size: 3692
PflMI CCAn_nnn'nTGG
Cuts at: 276 276
Size: 3692
PmeI GTTT'AAAC
Cuts at: 691 691
Size: 3692
PmlI CAC'GTG
Cuts at: 435 435
Size: 3692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 221 253 2831 2863 221
Size: 32 2578 32 1050
Fragments arranged by size:
2578 1050 32 32
PpuMI rG'GwC_Cy
Cuts at: 339 339
Size: 3692
PstI C_TGCA'G
Cuts at: 739 1424 739
Size: 685 3007
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3692
PvuII CAG'CTG
Cuts at: 1117 1477 1117
Size: 360 3332
RsrII CG'GwC_CG
Cuts at: 1887 1887
Size: 3692
SacI G_AGCT'C
Cuts at: 706 706
Size: 3692
SalI G'TCGA_C
Cuts at: 729 729
Size: 3692
SapI GCTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
SbfI CC_TGCA'GG
Cuts at: 739 739
Size: 3692
ScaI AGT'ACT
Cuts at: 3209 3209
Size: 3692
SfcI C'TryA_G
Cuts at: 33 735 1420 2413 2604 33
Size: 702 685 993 191 1121
Fragments arranged by size:
1121 993 702 685 191
SfoI GGC'GCC
Cuts at: 1124 1372 1124
Size: 248 3444
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
SmaI CCC'GGG
Cuts at: 714 714
Size: 3692
SmlI C'TyrA_G
Cuts at: 374 847 1762 2254 2516 374
Size: 473 915 492 262 1550
Fragments arranged by size:
1550 915 492 473 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3692
SphI G_CATG'C
Cuts at: 745 1245 745
Size: 500 3192
SspI AAT'ATT
Cuts at: 251 251
Size: 3692
StyI C'CwwG_G
Cuts at: 107 826 1180 107
Size: 719 354 2619
Fragments arranged by size:
2619 719 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 194 1275 1408 1867 2076 194
Size: 1081 133 459 209 1810
Fragments arranged by size:
1810 1081 459 209 133
TatI w'GTAC_w
Cuts at: 3207 3207
Size: 3692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 468 500 656 688 3209 3241 468
Size: 32 156 32 2521 32 919
Fragments arranged by size:
2521 919 156 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1489 1489
Size: 3692
XbaI T'CTAG_A
Cuts at: 723 723
Size: 3692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1187 1187
Size: 3692
XmaI C'CCGG_G
Cuts at: 712 712
Size: 3692
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI