Restriction map for
pF1KB8887
(Circular) (Six-base) MAPSORT of: pF1KB8887.seq Check: 8871 from: 1 to: 3707
pF1KB8887 3707 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 480 480
Size: 3707
AatII G_ACGT'C
Cuts at: 560 560
Size: 3707
AccI GT'mk_AC
Cuts at: 745 745
Size: 3707
Acc65I G'GTAC_C
Cuts at: 723 723
Size: 3707
AclI AA'CG_TT
Cuts at: 3547 3547
Size: 3707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 329 1532 1964 2711 2979 329
Size: 1203 432 747 268 1057
Fragments arranged by size:
1203 1057 747 432 268
AflII C'TTAA_G
Cuts at: 147 147
Size: 3707
AflIII A'CryG_T
Cuts at: 1059 2163 3140 1059
Size: 1104 977 1626
Fragments arranged by size:
1626 1104 977
AgeI A'CCGG_T
Cuts at: 2095 2095
Size: 3707
AlwNI CAG_nnn'CTG
Cuts at: 399 1012 2579 399
Size: 613 1567 1527
Fragments arranged by size:
1567 1527 613
ApaI G_GGCC'C
Cuts at: 340 340
Size: 3707
ApaLI G'TGCA_C
Cuts at: 2477 2477
Size: 3707
ApoI r'AATT_y
Cuts at: 711 3060 3316 711
Size: 2349 256 1102
Fragments arranged by size:
2349 1102 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3707
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
AvaI C'yCGr_G
Cuts at: 114 185 610 727 114
Size: 71 425 117 3094
Fragments arranged by size:
3094 425 117 71
BamHI G'GATC_C
Cuts at: 732 732
Size: 3707
BanI G'GyrC_C
Cuts at: 438 723 1137 1385 1420 438
Size: 285 414 248 35 2725
Fragments arranged by size:
2725 414 285 248 35
BanII G_rGCy'C
Cuts at: 128 340 721 1751 128
Size: 212 381 1030 2084
Fragments arranged by size:
2084 1030 381 212
BbeI G_GCGC'C
Cuts at: 442 1141 1389 442
Size: 699 248 2760
Fragments arranged by size:
2760 699 248
BbsI GAAGACnn'nnnn_
Cuts at: 414 414
Size: 3707
BciVI GTATCCnnnnn_n'
Cuts at: 1605 2366 3707 1605
Size: 761 1341 1605
Fragments arranged by size:
1605 1341 761
BclI T'GATC_A
Cuts at: 1227 1227
Size: 3707
BglI GCCn_nnn'nGGC
Cuts at: 650 650
Size: 3707
BglII A'GATC_T
Cuts at: 1222 3259 1222
Size: 2037 1670
BlpI GC'TnA_GC
Cuts at: 600 819 600
Size: 219 3488
Bme1580I G_kGCm'C
Cuts at: 340 1332 1425 2481 340
Size: 992 93 1056 1566
Fragments arranged by size:
1566 1056 992 93
BmrI ACTGGGnnnn_n'
Cuts at: 919 1000 1335 3479 919
Size: 81 335 2144 1147
Fragments arranged by size:
2144 1147 335 81
BmtI G_CTAG'C
Cuts at: 463 463
Size: 3707
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 541 2074 541
Size: 1533 2174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 421 427 605 271
Size: 150 6 178 3373
Fragments arranged by size:
3373 178 150 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 883 1762 2254 2552 511
Size: 372 879 492 298 1666
Fragments arranged by size:
1666 879 492 372 298
BsaI GGTCTCn'nnnn_
Cuts at: 3411 3411
Size: 3707
BsaAI yAC'GTr
Cuts at: 1060 1060
Size: 3707
BsaBI GATnn'nnATC
Cuts at: 6 1221 3258 6
Size: 1215 2037 455
Fragments arranged by size:
2037 1215 455
BsaHI Gr'CG_yC
Cuts at: 439 557 1138 1386 2088 3584 439
Size: 118 581 248 702 1496 562
Fragments arranged by size:
1496 702 581 562 248 118
BsaWI w'CCGG_w
Cuts at: 324 896 1417 2095 2369 2516 2927 324
Size: 572 521 678 274 147 411 1104
Fragments arranged by size:
1104 678 572 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 556 697 268
Size: 288 141 3278
Fragments arranged by size:
3278 288 141
BseYI C'CCAG_C
Cuts at: 333 1132 1890 2467 2963 333
Size: 799 758 577 496 1077
Fragments arranged by size:
1077 799 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 615 615
Size: 3707
BsiEI CG_ry'CG
Cuts at: 104 354 1249 1295 2503 104
Size: 250 895 46 1208 1308
Fragments arranged by size:
1308 1208 895 250 46
BsiHKAI G_wGCw'C
Cuts at: 192 721 1499 1689 2481 192
Size: 529 778 190 792 1418
Fragments arranged by size:
1418 792 778 529 190
BsmBI CGTCTCn'nnnn_
Cuts at: 446 446
Size: 3707
Bsp1286I G_dGCh'C
Cuts at: 128 192 340 721 1332 1425 1499 1689 1751
Size: 64 148 381 611 93 74 190 62
Cuts at: 1751 2481 128
Size: 730 1354
Fragments arranged by size:
1354 730 611 381 190 148 93 74
64 62
BspEI T'CCGG_A
Cuts at: 324 2927 324
Size: 2603 1104
BspHI T'CATG_A
Cuts at: 2883 2883
Size: 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 310 480 757 971 1273 1654 310
Size: 170 277 214 302 381 2363
Fragments arranged by size:
2363 381 302 277 214 170
BsrBI CCG'CTC
Cuts at: 50 411 1999 2053 3537 3702 50
Size: 361 1588 54 1484 165 55
Fragments arranged by size:
1588 1484 361 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1619 1619
Size: 3707
BsrFI r'CCGG_y
Cuts at: 512 649 1705 1886 2095 512
Size: 137 1056 181 209 2124
Fragments arranged by size:
2124 1056 209 181 137
BssHII G'CGCG_C
Cuts at: 245 289 291 549 245
Size: 44 2 258 3403
Fragments arranged by size:
3403 258 44 2
BssSI C'ACGA_G
Cuts at: 1978 2336 1978
Size: 358 3349
BstAPI GCAn_nnn'nTGC
Cuts at: 196 396 1084 196
Size: 200 688 2819
Fragments arranged by size:
2819 688 200
BstBI TT'CG_AA
Cuts at: 2068 2068
Size: 3707
BstYI r'GATC_y
Cuts at: 1 624 732 1199 1222 1557 1803 2815 2901
Size: 623 108 467 23 335 246 1012 86
Cuts at: 2901 2913 3259 1
Size: 12 346 449
Fragments arranged by size:
1012 623 467 449 346 335 246 108
86 23 12
BtgI C'CryG_G
Cuts at: 276 276
Size: 3707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 138 548 1657 1838 2001 138
Size: 410 1109 181 163 1844
Fragments arranged by size:
1844 1109 410 181 163
BtsI GCAGTG_nn'
Cuts at: 374 604 950 374
Size: 230 346 3131
Fragments arranged by size:
3131 346 230
DraI TTT'AAA
Cuts at: 706 706
Size: 3707
DrdI GACnn_nn'nnGTC
Cuts at: 1413 2271 1413
Size: 858 2849
EaeI y'GGCC_r
Cuts at: 351 479 1292 1466 1857 1884 3229 351
Size: 128 813 174 391 27 1345 829
Fragments arranged by size:
1345 829 813 391 174 128 27
EagI C'GGCC_G
Cuts at: 351 1292 351
Size: 941 2766
EarI CTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1915 2225 2371 3517 1915
Size: 310 146 1146 2105
Fragments arranged by size:
2105 1146 310 146
EcoICRI GAG'CTC
Cuts at: 719 719
Size: 3707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 329 421 427 605 1532 1964 2711 2979
Size: 58 92 6 178 927 432 747 268
Cuts at: 2979 271
Size: 999
Fragments arranged by size:
999 927 747 432 268 178 92 58
6
EcoO109I rG'GnC_Cy
Cuts at: 337 846 337
Size: 509 3198
EcoRI G'AATT_C
Cuts at: 711 711
Size: 3707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1726 1726
Size: 3707
FspI TGC'GCA
Cuts at: 1488 1488
Size: 3707
HaeII r_GCGC'y
Cuts at: 417 442 620 1141 1389 2411 3390 417
Size: 25 178 521 248 1022 979 734
Fragments arranged by size:
1022 979 734 521 248 178 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 547 579 1549 1579 1581 1611 547
Size: 32 970 30 2 30 2643
Fragments arranged by size:
2643 970 32 30 30 2
HincII GTy'rAC
Cuts at: 746 746
Size: 3707
KasI G'GCGC_C
Cuts at: 438 1137 1385 438
Size: 699 248 2760
Fragments arranged by size:
2760 699 248
KpnI G_GTAC'C
Cuts at: 727 727
Size: 3707
MluI A'CGCG_T
Cuts at: 3140 3140
Size: 3707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 253 2378 2562 253
Size: 2125 184 1398
Fragments arranged by size:
2125 1398 184
MscI TGG'CCA
Cuts at: 481 1468 481
Size: 987 2720
MslI CAynn'nnrTG
Cuts at: 1696 1823 1696
Size: 127 3580
MspA1I CmG'CkG
Cuts at: 240 278 818 1132 1492 2505 2750 240
Size: 38 540 314 360 1013 245 1197
Fragments arranged by size:
1197 1013 540 360 314 245 38
NaeI GCC'GGC
Cuts at: 651 1888 651
Size: 1237 2470
NarI GG'CG_CC
Cuts at: 439 1138 1386 439
Size: 699 248 2760
Fragments arranged by size:
2760 699 248
NgoMIV G'CCGG_C
Cuts at: 649 1886 649
Size: 1237 2470
NheI G'CTAG_C
Cuts at: 459 459
Size: 3707
NspI r_CATG'y
Cuts at: 760 1260 2167 760
Size: 500 907 2300
Fragments arranged by size:
2300 907 500
PciI A'CATG_T
Cuts at: 2163 2163
Size: 3707
PmeI GTTT'AAAC
Cuts at: 706 706
Size: 3707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 3675
PspOMI G'GGCC_C
Cuts at: 336 336
Size: 3707
PstI C_TGCA'G
Cuts at: 302 754 1439 302
Size: 452 685 2570
Fragments arranged by size:
2570 685 452
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3707
PvuII CAG'CTG
Cuts at: 1132 1492 1132
Size: 360 3347
RsrII CG'GwC_CG
Cuts at: 1902 1902
Size: 3707
SacI G_AGCT'C
Cuts at: 721 721
Size: 3707
SacII CC_GC'GG
Cuts at: 279 279
Size: 3707
SalI G'TCGA_C
Cuts at: 744 744
Size: 3707
SapI GCTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
SbfI CC_TGCA'GG
Cuts at: 754 754
Size: 3707
ScaI AGT'ACT
Cuts at: 3224 3224
Size: 3707
SfcI C'TryA_G
Cuts at: 33 298 750 1435 2428 2619 33
Size: 265 452 685 993 191 1121
Fragments arranged by size:
1121 993 685 452 265 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 650 650
Size: 3707
SfoI GGC'GCC
Cuts at: 440 1139 1387 440
Size: 699 248 2760
Fragments arranged by size:
2760 699 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
SmaI CCC'GGG
Cuts at: 729 729
Size: 3707
SmlI C'TyrA_G
Cuts at: 147 526 862 1777 2269 2531 147
Size: 379 336 915 492 262 1323
Fragments arranged by size:
1323 915 492 379 336 262
SpeI A'CTAG_T
Cuts at: 67 262 67
Size: 195 3512
SphI G_CATG'C
Cuts at: 760 1260 760
Size: 500 3207
StyI C'CwwG_G
Cuts at: 841 1195 841
Size: 354 3353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1290 1423 1882 2091 1290
Size: 133 459 209 2906
Fragments arranged by size:
2906 459 209 133
TatI w'GTAC_w
Cuts at: 3222 3222
Size: 3707
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 577 609 3224 3256 577
Size: 32 2615 32 1028
Fragments arranged by size:
2615 1028 32 32
Tth111I GACn'n_nGTC
Cuts at: 1504 1504
Size: 3707
XbaI T'CTAG_A
Cuts at: 738 738
Size: 3707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 489 1202 489
Size: 713 2994
XmaI C'CCGG_G
Cuts at: 727 727
Size: 3707
ZraI GAC'GTC
Cuts at: 558 558
Size: 3707
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PciI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AhdI AleI AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI Bpu10I BsaXI BsiWI BsmI
BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SanDI SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI