Restriction map for
pF1KB8894
(Circular) (Six-base) MAPSORT of: pF1KB8894.seq Check: 6927 from: 1 to: 3764
pF1KB8894 3764 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 802 802
Size: 3764
Acc65I G'GTAC_C
Cuts at: 780 780
Size: 3764
AclI AA'CG_TT
Cuts at: 3604 3604
Size: 3764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1589 2021 2768 3036 1589
Size: 432 747 268 2317
Fragments arranged by size:
2317 747 432 268
AflIII A'CryG_T
Cuts at: 1116 2220 3197 1116
Size: 1104 977 1683
Fragments arranged by size:
1683 1104 977
AgeI A'CCGG_T
Cuts at: 2152 2152
Size: 3764
AlwNI CAG_nnn'CTG
Cuts at: 209 1069 2636 209
Size: 860 1567 1337
Fragments arranged by size:
1567 1337 860
ApaI G_GGCC'C
Cuts at: 374 744 374
Size: 370 3394
ApaLI G'TGCA_C
Cuts at: 145 2534 145
Size: 2389 1375
ApoI r'AATT_y
Cuts at: 768 3117 3373 768
Size: 2349 256 1159
Fragments arranged by size:
2349 1159 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 259 304 375 784 259
Size: 45 71 409 3239
Fragments arranged by size:
3239 409 71 45
BamHI G'GATC_C
Cuts at: 789 789
Size: 3764
BanI G'GyrC_C
Cuts at: 319 354 364 412 780 1194 1442 1477 319
Size: 35 10 48 368 414 248 35 2606
Fragments arranged by size:
2606 414 368 248 48 35 35 10
BanII G_rGCy'C
Cuts at: 374 744 752 778 1808 374
Size: 370 8 26 1030 2330
Fragments arranged by size:
2330 1030 370 26 8
BbeI G_GCGC'C
Cuts at: 323 368 1198 1446 323
Size: 45 830 248 2641
Fragments arranged by size:
2641 830 248 45
BbsI GAAGACnn'nnnn_
Cuts at: 631 631
Size: 3764
BciVI GTATCCnnnnn_n'
Cuts at: 1662 2423 3764 1662
Size: 761 1341 1662
Fragments arranged by size:
1662 1341 761
BclI T'GATC_A
Cuts at: 1284 1284
Size: 3764
BglI GCCn_nnn'nGGC
Cuts at: 610 719 610
Size: 109 3655
BglII A'GATC_T
Cuts at: 1279 3316 1279
Size: 2037 1727
BlpI GC'TnA_GC
Cuts at: 876 876
Size: 3764
Bme1580I G_kGCm'C
Cuts at: 149 374 744 1389 1482 2538 149
Size: 225 370 645 93 1056 1375
Fragments arranged by size:
1375 1056 645 370 225 93
BmgBI CAC'GTC
Cuts at: 633 735 633
Size: 102 3662
BmrI ACTGGGnnnn_n'
Cuts at: 976 1057 1392 3536 976
Size: 81 335 2144 1204
Fragments arranged by size:
2144 1204 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 3764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 766
Size: 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 940 1819 2311 2609 736
Size: 204 879 492 298 1891
Fragments arranged by size:
1891 879 492 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 3468 3468
Size: 3764
BsaAI yAC'GTr
Cuts at: 1117 1117
Size: 3764
BsaBI GATnn'nnATC
Cuts at: 6 1278 3315 6
Size: 1272 2037 455
Fragments arranged by size:
2037 1272 455
BsaHI Gr'CG_yC
Cuts at: 320 365 1195 1443 2145 3641 320
Size: 45 830 248 702 1496 443
Fragments arranged by size:
1496 830 702 443 248 45
BsaWI w'CCGG_w
Cuts at: 953 1474 2152 2426 2573 2984 953
Size: 521 678 274 147 411 1733
Fragments arranged by size:
1733 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 655 235
Size: 420 3344
BseYI C'CCAG_C
Cuts at: 1189 1947 2524 3020 1189
Size: 758 577 496 1933
Fragments arranged by size:
1933 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1306 1352 2560 104
Size: 1202 46 1208 1308
Fragments arranged by size:
1308 1208 1202 46
BsiHKAI G_wGCw'C
Cuts at: 149 752 778 1556 1746 2538 149
Size: 603 26 778 190 792 1375
Fragments arranged by size:
1375 792 778 603 190 26
Bsp1286I G_dGCh'C
Cuts at: 149 374 744 752 778 1389 1482 1556 1746
Size: 225 370 8 26 611 93 74 190
Cuts at: 1746 1808 2538 149
Size: 62 730 1375
Fragments arranged by size:
1375 730 611 370 225 190 93 74
62 26 8
BspEI T'CCGG_A
Cuts at: 2984 2984
Size: 3764
BspHI T'CATG_A
Cuts at: 2940 2940
Size: 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 575 747 814 1028 1330 1711 575
Size: 172 67 214 302 381 2628
Fragments arranged by size:
2628 381 302 214 172 67
BsrBI CCG'CTC
Cuts at: 50 432 2056 2110 3594 3759 50
Size: 382 1624 54 1484 165 55
Fragments arranged by size:
1624 1484 382 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1676 1676
Size: 3764
BsrFI r'CCGG_y
Cuts at: 325 1762 1943 2152 325
Size: 1437 181 209 1937
Fragments arranged by size:
1937 1437 209 181
BssSI C'ACGA_G
Cuts at: 151 2035 2393 151
Size: 1884 358 1522
Fragments arranged by size:
1884 1522 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1141 1141
Size: 3764
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3764
BstYI r'GATC_y
Cuts at: 1 789 1256 1279 1614 1860 2872 2958 2970
Size: 788 467 23 335 246 1012 86 12
Cuts at: 2970 3316 1
Size: 346 449
Fragments arranged by size:
1012 788 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 237 237
Size: 3764
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 534 1714 1895 2058 534
Size: 1180 181 163 2240
Fragments arranged by size:
2240 1180 181 163
BtsI GCAGTG_nn'
Cuts at: 1007 1007
Size: 3764
DraI TTT'AAA
Cuts at: 763 763
Size: 3764
DraIII CAC_nnn'GTG
Cuts at: 141 141
Size: 3764
DrdI GACnn_nn'nnGTC
Cuts at: 1470 2328 1470
Size: 858 2906
EaeI y'GGCC_r
Cuts at: 252 536 572 724 1349 1523 1914 1941 3286
Size: 284 36 152 625 174 391 27 1345
Cuts at: 3286 252
Size: 730
Fragments arranged by size:
1345 730 625 391 284 174 152 36
27
EagI C'GGCC_G
Cuts at: 1349 1349
Size: 3764
EarI CTCTTCn'nnn_
Cuts at: 640 1787 1997 640
Size: 1147 210 2407
Fragments arranged by size:
2407 1147 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 613 1972 2282 2428 3574 613
Size: 1359 310 146 1146 803
Fragments arranged by size:
1359 1146 803 310 146
EcoICRI GAG'CTC
Cuts at: 750 776 750
Size: 26 3738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1589 2021 2768 3036 766
Size: 823 432 747 268 1494
Fragments arranged by size:
1494 823 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 371 741 903 371
Size: 370 162 3232
Fragments arranged by size:
3232 370 162
EcoRI G'AATT_C
Cuts at: 768 768
Size: 3764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1783 1783
Size: 3764
FspI TGC'GCA
Cuts at: 1545 1545
Size: 3764
HaeII r_GCGC'y
Cuts at: 323 368 408 461 1198 1446 2468 3447 323
Size: 45 40 53 737 248 1022 979 640
Fragments arranged by size:
1022 979 737 640 248 53 45 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1606 1636 1638 1668 1606
Size: 30 2 30 3702
Fragments arranged by size:
3702 30 30 2
HincII GTy'rAC
Cuts at: 803 803
Size: 3764
KasI G'GCGC_C
Cuts at: 319 364 1194 1442 319
Size: 45 830 248 2641
Fragments arranged by size:
2641 830 248 45
KpnI G_GTAC'C
Cuts at: 784 784
Size: 3764
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2435 2619 2435
Size: 184 3580
MscI TGG'CCA
Cuts at: 538 574 1525 538
Size: 36 951 2777
Fragments arranged by size:
2777 951 36
MslI CAynn'nnrTG
Cuts at: 1753 1880 1753
Size: 127 3637
MspA1I CmG'CkG
Cuts at: 187 209 317 875 1189 1549 2562 2807 187
Size: 22 108 558 314 360 1013 245 1144
Fragments arranged by size:
1144 1013 558 360 314 245 108 22
NaeI GCC'GGC
Cuts at: 327 1945 327
Size: 1618 2146
NarI GG'CG_CC
Cuts at: 320 365 1195 1443 320
Size: 45 830 248 2641
Fragments arranged by size:
2641 830 248 45
NcoI C'CATG_G
Cuts at: 237 237
Size: 3764
NgoMIV G'CCGG_C
Cuts at: 325 1943 325
Size: 1618 2146
NspI r_CATG'y
Cuts at: 817 1317 2224 817
Size: 500 907 2357
Fragments arranged by size:
2357 907 500
PciI A'CATG_T
Cuts at: 2220 2220
Size: 3764
PflMI CCAn_nnn'nTGG
Cuts at: 141 141
Size: 3764
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 3732
PspOMI G'GGCC_C
Cuts at: 370 740 370
Size: 370 3394
PstI C_TGCA'G
Cuts at: 214 811 1496 214
Size: 597 685 2482
Fragments arranged by size:
2482 685 597
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 187 1189 1549 187
Size: 1002 360 2402
Fragments arranged by size:
2402 1002 360
RsrII CG'GwC_CG
Cuts at: 1959 1959
Size: 3764
SacI G_AGCT'C
Cuts at: 752 778 752
Size: 26 3738
SalI G'TCGA_C
Cuts at: 801 801
Size: 3764
SapI GCTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
SbfI CC_TGCA'GG
Cuts at: 811 811
Size: 3764
ScaI AGT'ACT
Cuts at: 3281 3281
Size: 3764
SfcI C'TryA_G
Cuts at: 33 210 279 297 807 1492 2485 2676 33
Size: 177 69 18 510 685 993 191 1121
Fragments arranged by size:
1121 993 685 510 191 177 69 18
SfoI GGC'GCC
Cuts at: 321 366 1196 1444 321
Size: 45 830 248 2641
Fragments arranged by size:
2641 830 248 45
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SmaI CCC'GGG
Cuts at: 377 786 377
Size: 409 3355
SmlI C'TyrA_G
Cuts at: 751 919 1834 2326 2588 751
Size: 168 915 492 262 1927
Fragments arranged by size:
1927 915 492 262 168
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 817 1317 817
Size: 500 3264
StuI AGG'CCT
Cuts at: 604 604
Size: 3764
StyI C'CwwG_G
Cuts at: 237 575 898 1252 237
Size: 338 323 354 2749
Fragments arranged by size:
2749 354 338 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 643 1347 1480 1939 2148 643
Size: 704 133 459 209 2259
Fragments arranged by size:
2259 704 459 209 133
TatI w'GTAC_w
Cuts at: 3279 3279
Size: 3764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 3732
Tth111I GACn'n_nGTC
Cuts at: 1561 1561
Size: 3764
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1259 1259
Size: 3764
XmaI C'CCGG_G
Cuts at: 375 784 375
Size: 409 3355
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI