Restriction map for
pF1KB8903
(Circular) (Six-base) MAPSORT of: pF1KB8903.seq Check: 5952 from: 1 to: 3818
pF1KB8903 3818 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AatII G_ACGT'C
Cuts at: 167 167
Size: 3818
AccI GT'mk_AC
Cuts at: 856 856
Size: 3818
Acc65I G'GTAC_C
Cuts at: 557 834 557
Size: 277 3541
AclI AA'CG_TT
Cuts at: 3658 3658
Size: 3818
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1643 2075 2822 3090 1643
Size: 432 747 268 2371
Fragments arranged by size:
2371 747 432 268
AflIII A'CryG_T
Cuts at: 1170 2274 3251 1170
Size: 1104 977 1737
Fragments arranged by size:
1737 1104 977
AgeI A'CCGG_T
Cuts at: 2206 2206
Size: 3818
AlwNI CAG_nnn'CTG
Cuts at: 567 1123 2690 567
Size: 556 1567 1695
Fragments arranged by size:
1695 1567 556
ApaLI G'TGCA_C
Cuts at: 2588 2588
Size: 3818
ApoI r'AATT_y
Cuts at: 111 495 583 822 3171 3427 111
Size: 384 88 239 2349 256 502
Fragments arranged by size:
2349 502 384 256 239 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 3818
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3818
AvaI C'yCGr_G
Cuts at: 726 838 726
Size: 112 3706
BamHI G'GATC_C
Cuts at: 843 843
Size: 3818
BanI G'GyrC_C
Cuts at: 557 611 834 1248 1496 1531 557
Size: 54 223 414 248 35 2844
Fragments arranged by size:
2844 414 248 223 54 35
BanII G_rGCy'C
Cuts at: 832 1862 832
Size: 1030 2788
BbeI G_GCGC'C
Cuts at: 615 1252 1500 615
Size: 637 248 2933
Fragments arranged by size:
2933 637 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 609 643 609
Size: 34 3784
BciVI GTATCCnnnnn_n'
Cuts at: 475 1716 2477 3818 475
Size: 1241 761 1341 475
Fragments arranged by size:
1341 1241 761 475
BclI T'GATC_A
Cuts at: 1338 1338
Size: 3818
BglII A'GATC_T
Cuts at: 545 1333 3370 545
Size: 788 2037 993
Fragments arranged by size:
2037 993 788
BlpI GC'TnA_GC
Cuts at: 930 930
Size: 3818
Bme1580I G_kGCm'C
Cuts at: 1443 1536 2592 1443
Size: 93 1056 2669
Fragments arranged by size:
2669 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1030 1111 1446 3590 1030
Size: 81 335 2144 1258
Fragments arranged by size:
2144 1258 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 3818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 595
Size: 3818
Bpu10I CC'TnA_GC
Cuts at: 410 410
Size: 3818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 1873 2365 2663 994
Size: 879 492 298 2149
Fragments arranged by size:
2149 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3522 3522
Size: 3818
BsaAI yAC'GTr
Cuts at: 1171 1171
Size: 3818
BsaBI GATnn'nnATC
Cuts at: 6 664 1332 3369 6
Size: 658 668 2037 455
Fragments arranged by size:
2037 668 658 455
BsaHI Gr'CG_yC
Cuts at: 164 612 1249 1497 2199 3695 164
Size: 448 637 248 702 1496 287
Fragments arranged by size:
1496 702 637 448 287 248
BsaWI w'CCGG_w
Cuts at: 248 527 1007 1528 2206 2480 2627 3038 248
Size: 279 480 521 678 274 147 411 1028
Fragments arranged by size:
1028 678 521 480 411 279 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 221 251 221
Size: 30 3788
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 811 811
Size: 3818
BseYI C'CCAG_C
Cuts at: 1243 2001 2578 3074 1243
Size: 758 577 496 1987
Fragments arranged by size:
1987 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 236 540 1360 1406 2614 104
Size: 132 304 820 46 1208 1308
Fragments arranged by size:
1308 1208 820 304 132 46
BsiHKAI G_wGCw'C
Cuts at: 832 1610 1800 2592 832
Size: 778 190 792 2058
Fragments arranged by size:
2058 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 832 1443 1536 1610 1800 1862 2592 832
Size: 611 93 74 190 62 730 2058
Fragments arranged by size:
2058 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3038 3038
Size: 3818
BspHI T'CATG_A
Cuts at: 2994 2994
Size: 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 868 1082 1384 1765 868
Size: 214 302 381 2921
Fragments arranged by size:
2921 381 302 214
BsrBI CCG'CTC
Cuts at: 50 232 523 2110 2164 3648 3813 50
Size: 182 291 1587 54 1484 165 55
Fragments arranged by size:
1587 1484 291 182 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1730 1730
Size: 3818
BsrFI r'CCGG_y
Cuts at: 614 1816 1997 2206 614
Size: 1202 181 209 2226
Fragments arranged by size:
2226 1202 209 181
BssSI C'ACGA_G
Cuts at: 2089 2447 2089
Size: 358 3460
BstAPI GCAn_nnn'nTGC
Cuts at: 1195 1195
Size: 3818
BstBI TT'CG_AA
Cuts at: 2179 2179
Size: 3818
BstYI r'GATC_y
Cuts at: 1 251 325 545 843 1310 1333 1668 1914
Size: 250 74 220 298 467 23 335 246
Cuts at: 1914 2926 3012 3024 3370 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 298 250 246
220 86 74 23 12
Bsu36I CC'TnA_GG
Cuts at: 381 381
Size: 3818
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1768 1949 2112 1768
Size: 181 163 3474
Fragments arranged by size:
3474 181 163
BtsI GCAGTG_nn'
Cuts at: 1061 1061
Size: 3818
DraI TTT'AAA
Cuts at: 817 817
Size: 3818
DrdI GACnn_nn'nnGTC
Cuts at: 1524 2382 1524
Size: 858 2960
EaeI y'GGCC_r
Cuts at: 233 537 1403 1577 1968 1995 3340 233
Size: 304 866 174 391 27 1345 711
Fragments arranged by size:
1345 866 711 391 304 174 27
EagI C'GGCC_G
Cuts at: 233 537 1403 233
Size: 304 866 2648
Fragments arranged by size:
2648 866 304
EarI CTCTTCn'nnn_
Cuts at: 779 788 1841 2051 779
Size: 9 1053 210 2546
Fragments arranged by size:
2546 1053 210 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 752 779 2026 2336 2482 3628 752
Size: 27 1247 310 146 1146 942
Fragments arranged by size:
1247 1146 942 310 146 27
EcoICRI GAG'CTC
Cuts at: 830 830
Size: 3818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 1643 2075 2822 3090 595
Size: 1048 432 747 268 1323
Fragments arranged by size:
1323 1048 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 957 957
Size: 3818
EcoRI G'AATT_C
Cuts at: 111 495 822 111
Size: 384 327 3107
Fragments arranged by size:
3107 384 327
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1837 1837
Size: 3818
FspI TGC'GCA
Cuts at: 1599 1599
Size: 3818
HaeII r_GCGC'y
Cuts at: 615 1252 1500 2522 3501 615
Size: 637 248 1022 979 932
Fragments arranged by size:
1022 979 932 637 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 310 342 343 375 1660 1690 1692 1722 310
Size: 32 1 32 1285 30 2 30 2406
Fragments arranged by size:
2406 1285 32 32 30 30 2 1
HincII GTy'rAC
Cuts at: 857 857
Size: 3818
KasI G'GCGC_C
Cuts at: 611 1248 1496 611
Size: 637 248 2933
Fragments arranged by size:
2933 637 248
KpnI G_GTAC'C
Cuts at: 561 838 561
Size: 277 3541
MluI A'CGCG_T
Cuts at: 3251 3251
Size: 3818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2489 2673 2489
Size: 184 3634
MscI TGG'CCA
Cuts at: 1579 1579
Size: 3818
MslI CAynn'nnrTG
Cuts at: 1807 1934 1807
Size: 127 3691
MspA1I CmG'CkG
Cuts at: 929 1243 1603 2616 2861 929
Size: 314 360 1013 245 1886
Fragments arranged by size:
1886 1013 360 314 245
NaeI GCC'GGC
Cuts at: 616 1999 616
Size: 1383 2435
NarI GG'CG_CC
Cuts at: 612 1249 1497 612
Size: 637 248 2933
Fragments arranged by size:
2933 637 248
NgoMIV G'CCGG_C
Cuts at: 614 1997 614
Size: 1383 2435
NotI GC'GGCC_GC
Cuts at: 537 537
Size: 3818
NspI r_CATG'y
Cuts at: 357 871 1371 2278 357
Size: 514 500 907 1897
Fragments arranged by size:
1897 907 514 500
PciI A'CATG_T
Cuts at: 2274 2274
Size: 3818
PflMI CCAn_nnn'nTGG
Cuts at: 567 567
Size: 3818
PmeI GTTT'AAAC
Cuts at: 817 817
Size: 3818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 2989 2957
Size: 32 3786
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 238 270 238
Size: 32 3786
PstI C_TGCA'G
Cuts at: 370 865 1550 370
Size: 495 685 2638
Fragments arranged by size:
2638 685 495
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3818
PvuII CAG'CTG
Cuts at: 1243 1603 1243
Size: 360 3458
RsrII CG'GwC_CG
Cuts at: 525 2013 525
Size: 1488 2330
SacI G_AGCT'C
Cuts at: 832 832
Size: 3818
SalI G'TCGA_C
Cuts at: 855 855
Size: 3818
SapI GCTCTTCn'nnn_
Cuts at: 1841 2051 1841
Size: 210 3608
SbfI CC_TGCA'GG
Cuts at: 865 865
Size: 3818
ScaI AGT'ACT
Cuts at: 3335 3335
Size: 3818
SfcI C'TryA_G
Cuts at: 33 366 861 1546 2539 2730 33
Size: 333 495 685 993 191 1121
Fragments arranged by size:
1121 993 685 495 333 191
SfoI GGC'GCC
Cuts at: 613 1250 1498 613
Size: 637 248 2933
Fragments arranged by size:
2933 637 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3818
SgrAI Cr'CCGG_yG
Cuts at: 614 614
Size: 3818
SmaI CCC'GGG
Cuts at: 840 840
Size: 3818
SmlI C'TyrA_G
Cuts at: 973 1888 2380 2642 973
Size: 915 492 262 2149
Fragments arranged by size:
2149 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3818
SphI G_CATG'C
Cuts at: 871 1371 871
Size: 500 3318
StyI C'CwwG_G
Cuts at: 952 1306 952
Size: 354 3464
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 664 1401 1534 1993 2202 664
Size: 737 133 459 209 2280
Fragments arranged by size:
2280 737 459 209 133
TatI w'GTAC_w
Cuts at: 3333 3333
Size: 3818
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3335 3367 3335
Size: 32 3786
Tth111I GACn'n_nGTC
Cuts at: 1615 1615
Size: 3818
XbaI T'CTAG_A
Cuts at: 849 849
Size: 3818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1313 1313
Size: 3818
XmaI C'CCGG_G
Cuts at: 838 838
Size: 3818
XmnI GAAnn'nnTTC
Cuts at: 495 495
Size: 3818
ZraI GAC'GTC
Cuts at: 165 165
Size: 3818
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NotI NspI PciI
PflMI PmeI PpiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XhoI