Restriction map for
pF1KB8904
(Circular) (Six-base) MAPSORT of: pF1KB8904.seq Check: 8297 from: 1 to: 3824
pF1KB8904 3824 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 862 862
Size: 3824
Acc65I G'GTAC_C
Cuts at: 840 840
Size: 3824
AclI AA'CG_TT
Cuts at: 3664 3664
Size: 3824
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 675 733 1649 2081 2828 3096 675
Size: 58 916 432 747 268 1403
Fragments arranged by size:
1403 916 747 432 268 58
AflIII A'CryG_T
Cuts at: 1176 2280 3257 1176
Size: 1104 977 1743
Fragments arranged by size:
1743 1104 977
AgeI A'CCGG_T
Cuts at: 2212 2212
Size: 3824
AleI CACnn'nnGTG
Cuts at: 475 475
Size: 3824
AlwNI CAG_nnn'CTG
Cuts at: 708 1129 2696 708
Size: 421 1567 1836
Fragments arranged by size:
1836 1567 421
ApaLI G'TGCA_C
Cuts at: 2594 2594
Size: 3824
ApoI r'AATT_y
Cuts at: 828 3177 3433 828
Size: 2349 256 1219
Fragments arranged by size:
2349 1219 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3824
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3824
AvaI C'yCGr_G
Cuts at: 844 844
Size: 3824
BamHI G'GATC_C
Cuts at: 849 849
Size: 3824
BanI G'GyrC_C
Cuts at: 840 1254 1502 1537 840
Size: 414 248 35 3127
Fragments arranged by size:
3127 414 248 35
BanII G_rGCy'C
Cuts at: 838 1868 838
Size: 1030 2794
BbeI G_GCGC'C
Cuts at: 1258 1506 1258
Size: 248 3576
BbvCI CC'TCA_GC
Cuts at: 617 617
Size: 3824
BciVI GTATCCnnnnn_n'
Cuts at: 1722 2483 3824 1722
Size: 761 1341 1722
Fragments arranged by size:
1722 1341 761
BclI T'GATC_A
Cuts at: 1344 1344
Size: 3824
BglII A'GATC_T
Cuts at: 1339 3376 1339
Size: 2037 1787
BlpI GC'TnA_GC
Cuts at: 262 936 262
Size: 674 3150
Bme1580I G_kGCm'C
Cuts at: 1449 1542 2598 1449
Size: 93 1056 2675
Fragments arranged by size:
2675 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1036 1117 1452 3596 1036
Size: 81 335 2144 1264
Fragments arranged by size:
2144 1264 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 3824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 580
Size: 3824
Bpu10I CC'TnA_GC
Cuts at: 617 617
Size: 3824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1879 2371 2669 1000
Size: 879 492 298 2155
Fragments arranged by size:
2155 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3528 3528
Size: 3824
BsaAI yAC'GTr
Cuts at: 550 1177 550
Size: 627 3197
BsaBI GATnn'nnATC
Cuts at: 6 1338 3375 6
Size: 1332 2037 455
Fragments arranged by size:
2037 1332 455
BsaHI Gr'CG_yC
Cuts at: 1255 1503 2205 3701 1255
Size: 248 702 1496 1378
Fragments arranged by size:
1496 1378 702 248
BsaWI w'CCGG_w
Cuts at: 1013 1534 2212 2486 2633 3044 1013
Size: 521 678 274 147 411 1793
Fragments arranged by size:
1793 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 547 577 547
Size: 30 3794
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 133
Size: 3824
BseYI C'CCAG_C
Cuts at: 462 1249 2007 2584 3080 462
Size: 787 758 577 496 1206
Fragments arranged by size:
1206 787 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 766
Size: 3824
BsiEI CG_ry'CG
Cuts at: 104 1366 1412 2620 104
Size: 1262 46 1208 1308
Fragments arranged by size:
1308 1262 1208 46
BsiHKAI G_wGCw'C
Cuts at: 482 499 838 1616 1806 2598 482
Size: 17 339 778 190 792 1708
Fragments arranged by size:
1708 792 778 339 190 17
Bsp1286I G_dGCh'C
Cuts at: 482 499 838 1449 1542 1616 1806 1868 2598
Size: 17 339 611 93 74 190 62 730
Cuts at: 2598 482
Size: 1708
Fragments arranged by size:
1708 730 611 339 190 93 74 62
17
BspEI T'CCGG_A
Cuts at: 3044 3044
Size: 3824
BspHI T'CATG_A
Cuts at: 3000 3000
Size: 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 188 874 1088 1390 1771 188
Size: 686 214 302 381 2241
Fragments arranged by size:
2241 686 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2116 2170 3654 3819 50
Size: 2066 54 1484 165 55
Fragments arranged by size:
2066 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1736 1736
Size: 3824
BsrFI r'CCGG_y
Cuts at: 1822 2003 2212 1822
Size: 181 209 3434
Fragments arranged by size:
3434 209 181
BssSI C'ACGA_G
Cuts at: 2095 2453 2095
Size: 358 3466
BstAPI GCAn_nnn'nTGC
Cuts at: 1201 1201
Size: 3824
BstBI TT'CG_AA
Cuts at: 2185 2185
Size: 3824
BstEII G'GTnAC_C
Cuts at: 579 579
Size: 3824
BstXI CCAn_nnnn'nTGG
Cuts at: 576 576
Size: 3824
BstYI r'GATC_y
Cuts at: 1 200 849 1316 1339 1674 1920 2932 3018
Size: 199 649 467 23 335 246 1012 86
Cuts at: 3018 3030 3376 1
Size: 12 346 449
Fragments arranged by size:
1012 649 467 449 346 335 246 199
86 23 12
BtgI C'CryG_G
Cuts at: 393 426 393
Size: 33 3791
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1774 1955 2118 1774
Size: 181 163 3480
Fragments arranged by size:
3480 181 163
BtsI GCAGTG_nn'
Cuts at: 1067 1067
Size: 3824
DraI TTT'AAA
Cuts at: 823 823
Size: 3824
DrdI GACnn_nn'nnGTC
Cuts at: 285 1530 2388 285
Size: 1245 858 1721
Fragments arranged by size:
1721 1245 858
EaeI y'GGCC_r
Cuts at: 1409 1583 1974 2001 3346 1409
Size: 174 391 27 1345 1887
Fragments arranged by size:
1887 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1409 1409
Size: 3824
EarI CTCTTCn'nnn_
Cuts at: 649 1847 2057 649
Size: 1198 210 2416
Fragments arranged by size:
2416 1198 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2032 2342 2488 3634 2032
Size: 310 146 1146 2222
Fragments arranged by size:
2222 1146 310 146
EcoICRI GAG'CTC
Cuts at: 836 836
Size: 3824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 675 733 1649 2081 2828 3096 580
Size: 95 58 916 432 747 268 1308
Fragments arranged by size:
1308 916 747 432 268 95 58
EcoNI CCTnn'n_nnAGG
Cuts at: 184 184
Size: 3824
EcoO109I rG'GnC_Cy
Cuts at: 762 963 762
Size: 201 3623
EcoRI G'AATT_C
Cuts at: 828 828
Size: 3824
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1843 1843
Size: 3824
FspI TGC'GCA
Cuts at: 1605 1605
Size: 3824
HaeII r_GCGC'y
Cuts at: 724 1258 1506 2528 3507 724
Size: 534 248 1022 979 1041
Fragments arranged by size:
1041 1022 979 534 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 547 579 1666 1696 1698 1728 547
Size: 32 1087 30 2 30 2643
Fragments arranged by size:
2643 1087 32 30 30 2
HincII GTy'rAC
Cuts at: 863 863
Size: 3824
KasI G'GCGC_C
Cuts at: 1254 1502 1254
Size: 248 3576
KpnI G_GTAC'C
Cuts at: 844 844
Size: 3824
MluI A'CGCG_T
Cuts at: 3257 3257
Size: 3824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 2495 2679 92
Size: 2403 184 1237
Fragments arranged by size:
2403 1237 184
MscI TGG'CCA
Cuts at: 1585 1585
Size: 3824
MslI CAynn'nnrTG
Cuts at: 475 1813 1940 475
Size: 1338 127 2359
Fragments arranged by size:
2359 1338 127
MspA1I CmG'CkG
Cuts at: 395 705 935 1249 1609 2622 2867 395
Size: 310 230 314 360 1013 245 1352
Fragments arranged by size:
1352 1013 360 314 310 245 230
NaeI GCC'GGC
Cuts at: 2005 2005
Size: 3824
NarI GG'CG_CC
Cuts at: 1255 1503 1255
Size: 248 3576
NgoMIV G'CCGG_C
Cuts at: 2003 2003
Size: 3824
NspI r_CATG'y
Cuts at: 348 877 1377 2284 348
Size: 529 500 907 1888
Fragments arranged by size:
1888 907 529 500
PciI A'CATG_T
Cuts at: 2280 2280
Size: 3824
PflMI CCAn_nnn'nTGG
Cuts at: 708 708
Size: 3824
PfoI T'CCnGG_A
Cuts at: 206 206
Size: 3824
PmeI GTTT'AAAC
Cuts at: 823 823
Size: 3824
PmlI CAC'GTG
Cuts at: 550 550
Size: 3824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 3792
PpuMI rG'GwC_Cy
Cuts at: 762 762
Size: 3824
PstI C_TGCA'G
Cuts at: 871 1556 871
Size: 685 3139
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3824
PvuII CAG'CTG
Cuts at: 705 1249 1609 705
Size: 544 360 2920
Fragments arranged by size:
2920 544 360
RsrII CG'GwC_CG
Cuts at: 2019 2019
Size: 3824
SacI G_AGCT'C
Cuts at: 838 838
Size: 3824
SacII CC_GC'GG
Cuts at: 396 396
Size: 3824
SalI G'TCGA_C
Cuts at: 861 861
Size: 3824
SapI GCTCTTCn'nnn_
Cuts at: 1847 2057 1847
Size: 210 3614
SbfI CC_TGCA'GG
Cuts at: 871 871
Size: 3824
ScaI AGT'ACT
Cuts at: 3341 3341
Size: 3824
SfcI C'TryA_G
Cuts at: 33 724 867 1552 2545 2736 33
Size: 691 143 685 993 191 1121
Fragments arranged by size:
1121 993 691 685 191 143
SfoI GGC'GCC
Cuts at: 1256 1504 1256
Size: 248 3576
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3824
SmaI CCC'GGG
Cuts at: 846 846
Size: 3824
SmlI C'TyrA_G
Cuts at: 979 1894 2386 2648 979
Size: 915 492 262 2155
Fragments arranged by size:
2155 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3824
SphI G_CATG'C
Cuts at: 877 1377 877
Size: 500 3324
StuI AGG'CCT
Cuts at: 140 406 140
Size: 266 3558
StyI C'CwwG_G
Cuts at: 185 958 1312 185
Size: 773 354 2697
Fragments arranged by size:
2697 773 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 675 1407 1540 1999 2208 675
Size: 732 133 459 209 2291
Fragments arranged by size:
2291 732 459 209 133
TatI w'GTAC_w
Cuts at: 218 3339 218
Size: 3121 703
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 3792
Tth111I GACn'n_nGTC
Cuts at: 1621 1621
Size: 3824
XbaI T'CTAG_A
Cuts at: 855 855
Size: 3824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1319 1319
Size: 3824
XmaI C'CCGG_G
Cuts at: 844 844
Size: 3824
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI